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The map label for this gene is fumB [H]

Identifier: 29347666

GI number: 29347666

Start: 2828639

End: 2830273

Strand: Reverse

Name: fumB [H]

Synonym: BT_2256

Alternate gene names: 29347666

Gene position: 2830273-2828639 (Counterclockwise)

Preceding gene: 29347667

Following gene: 29347665

Centisome position: 45.21

GC content: 46.54

Gene sequence:

>1635_bases
ATGGCAACACCTCCGTTTAAGTATCAACCCATGTTTGAAAAGGGAAAAGATACCACTGAGTATTATCTGCTTACAAAAGA
CTATGTATCGGTAAGCGAGTTTGAAGGAAATCCTATCCTGAAAATTGAGAAAGAAGGCTTGACTGCGATGGCAAATGCAG
CTTTTCGTGATGTGTCATTCATGCTTCGCCGTTCGCACAATGAACAGGTTGCTAAGATTCTGAGTGATCCGGAAGCCAGC
GAGAACGATAAGTACGTAGCACTGACTTTCCTGCGTAATGCGGAAGTGGCTGCCAAAGGTGTACTTCCATTCTGTCAGGA
TACCGGTACTGCTATTATCCACGGTGAAAAGGGACAGCAAGTGTGGACCGGCTATACCGACGAGGAAGCTCTGTCACTGG
GTGTTTACAAAACATATACCGAAGAAAACCTGCGTTACTCTCAGAATGCGCCTCTGAATATGTATGATGAGGTAAATACA
AAATGTAACCTGCCTGCACAGATTGACATCGAAGCTACCGAAGGGATGGAATATGAATTCCTCTGCGTAACGAAAGGTGG
TGGTTCTGCCAACAAAACATATCTGTATCAGGAAACAAAAGCAATTTTGAACCCCGGTACACTGGTTCCGTTCCTGGTGG
AAAAAATGAAGACTCTGGGAACTGCCGCTTGTCCTCCTTATCATATCGCTTTTGTGATCGGCGGTACTTCTGCTGAGAAG
AACCTGCTGACAGTGAAACTGGCGTCTACTCACTTCTACGATAATCTGCCGACTACGGGAAATGAATTTGGCCGTGCTTT
CCGTGATATCGAACTGGAAAAACAAGTACTGGAAGAAGCTCACAAGATCGGTCTGGGTGCACAATTCGGCGGTAAATATC
TGGCACATGATGTTCGTATCATTCGTCTGCCTCGTCACGGTGCTTCTTGTCCGGTAGGTCTGGGCGTGTCTTGTTCTGCC
GACCGTAACATCAAATGTAAAATCAATAAAGATGGTATCTGGATTGAGAAACTGGATTCAAATCCGGGTAGCCTGATTCC
GGCAGAATTGCGTCAGGCCGGTGAAGGTGATGTAGTGAAGATTGATCTGAACCGTCCGATGGCTGAGATTCTGAAAGAAC
TGACCAAATATCCTGTATCTACTCGTCTGTCACTGAACGGTACTATCATCGTTGGCCGTGATATCGCTCACGCTAAACTG
AAAGAACGTCTGGATCGTGGAGAAGATTTGCCTCAGTATATCAAGGATCATCCTATTTACTATGCTGGTCCGGCCAAGAC
TCCGGCTGGTATGGCTTGCGGTTCAATGGGCCCGACAACTGCCGGACGTATGGACTCGTATGTTGAGCTGTTCCAGAGCC
ACGGTGGCAGCATGGTTATGTTGGCGAAAGGTAACCGTAGCCAGCAGGTTACAGATGCTTGTCAGAAATATGGCGGCTTC
TATCTGGGTTCTATCGGCGGACCGGCTGCAATCCTTGCTCAGAACAACATCAAGAGCATTGAGTGCGTAGAATATCCGGA
ACTGGGCATGGAAGCAATCTGGAAAATTGAAGTGGAAGATTTCCCGGCATTTATTCTGGTAGATGACAAAGGAAATGACT
TCTTCAAACAGATTAAACCACGTTGTGCCAAATAA

Upstream 100 bases:

>100_bases
CGTTATTCATTCTTAATTCTTCATTCTTCCGTTATCATTCTTTATTTTTAATTACTACCTTTGCCTCACTAACTAACATT
ACGTAATAAAATAAGAAATT

Downstream 100 bases:

>100_bases
CAAATAACAAGTAATAAGTAACAAGTAATAAGTAATGGCAAGTTATTCTTGCGGTTGATTACATAAACATTCATTTGGGA
TACCTGTCAGTTTTCGTATG

Product: fumarate hydratase class I, anaerobic

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MATPPFKYQPMFEKGKDTTEYYLLTKDYVSVSEFEGNPILKIEKEGLTAMANAAFRDVSFMLRRSHNEQVAKILSDPEAS
ENDKYVALTFLRNAEVAAKGVLPFCQDTGTAIIHGEKGQQVWTGYTDEEALSLGVYKTYTEENLRYSQNAPLNMYDEVNT
KCNLPAQIDIEATEGMEYEFLCVTKGGGSANKTYLYQETKAILNPGTLVPFLVEKMKTLGTAACPPYHIAFVIGGTSAEK
NLLTVKLASTHFYDNLPTTGNEFGRAFRDIELEKQVLEEAHKIGLGAQFGGKYLAHDVRIIRLPRHGASCPVGLGVSCSA
DRNIKCKINKDGIWIEKLDSNPGSLIPAELRQAGEGDVVKIDLNRPMAEILKELTKYPVSTRLSLNGTIIVGRDIAHAKL
KERLDRGEDLPQYIKDHPIYYAGPAKTPAGMACGSMGPTTAGRMDSYVELFQSHGGSMVMLAKGNRSQQVTDACQKYGGF
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFKQIKPRCAK

Sequences:

>Translated_544_residues
MATPPFKYQPMFEKGKDTTEYYLLTKDYVSVSEFEGNPILKIEKEGLTAMANAAFRDVSFMLRRSHNEQVAKILSDPEAS
ENDKYVALTFLRNAEVAAKGVLPFCQDTGTAIIHGEKGQQVWTGYTDEEALSLGVYKTYTEENLRYSQNAPLNMYDEVNT
KCNLPAQIDIEATEGMEYEFLCVTKGGGSANKTYLYQETKAILNPGTLVPFLVEKMKTLGTAACPPYHIAFVIGGTSAEK
NLLTVKLASTHFYDNLPTTGNEFGRAFRDIELEKQVLEEAHKIGLGAQFGGKYLAHDVRIIRLPRHGASCPVGLGVSCSA
DRNIKCKINKDGIWIEKLDSNPGSLIPAELRQAGEGDVVKIDLNRPMAEILKELTKYPVSTRLSLNGTIIVGRDIAHAKL
KERLDRGEDLPQYIKDHPIYYAGPAKTPAGMACGSMGPTTAGRMDSYVELFQSHGGSMVMLAKGNRSQQVTDACQKYGGF
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFKQIKPRCAK
>Mature_543_residues
ATPPFKYQPMFEKGKDTTEYYLLTKDYVSVSEFEGNPILKIEKEGLTAMANAAFRDVSFMLRRSHNEQVAKILSDPEASE
NDKYVALTFLRNAEVAAKGVLPFCQDTGTAIIHGEKGQQVWTGYTDEEALSLGVYKTYTEENLRYSQNAPLNMYDEVNTK
CNLPAQIDIEATEGMEYEFLCVTKGGGSANKTYLYQETKAILNPGTLVPFLVEKMKTLGTAACPPYHIAFVIGGTSAEKN
LLTVKLASTHFYDNLPTTGNEFGRAFRDIELEKQVLEEAHKIGLGAQFGGKYLAHDVRIIRLPRHGASCPVGLGVSCSAD
RNIKCKINKDGIWIEKLDSNPGSLIPAELRQAGEGDVVKIDLNRPMAEILKELTKYPVSTRLSLNGTIIVGRDIAHAKLK
ERLDRGEDLPQYIKDHPIYYAGPAKTPAGMACGSMGPTTAGRMDSYVELFQSHGGSMVMLAKGNRSQQVTDACQKYGGFY
LGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFKQIKPRCAK

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=544, Percent_Identity=77.3897058823529, Blast_Score=887, Evalue=0.0,
Organism=Escherichia coli, GI1787897, Length=548, Percent_Identity=76.0948905109489, Blast_Score=876, Evalue=0.0,
Organism=Escherichia coli, GI1789443, Length=172, Percent_Identity=30.8139534883721, Blast_Score=79, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 59985; Mature: 59854

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPPFKYQPMFEKGKDTTEYYLLTKDYVSVSEFEGNPILKIEKEGLTAMANAAFRDVSF
CCCCCCCCCCHHHCCCCCEEEEEEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHH
MLRRSHNEQVAKILSDPEASENDKYVALTFLRNAEVAAKGVLPFCQDTGTAIIHGEKGQQ
HHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHCCCCCCCCCCCEEEECCCCCE
VWTGYTDEEALSLGVYKTYTEENLRYSQNAPLNMYDEVNTKCNLPAQIDIEATEGMEYEF
EECCCCCHHHHEECEEEEECCCCCEECCCCCCCCHHCCCCCCCCCEEEEEEECCCCCEEE
LCVTKGGGSANKTYLYQETKAILNPGTLVPFLVEKMKTLGTAACPPYHIAFVIGGTSAEK
EEEECCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLLTVKLASTHFYDNLPTTGNEFGRAFRDIELEKQVLEEAHKIGLGAQFGGKYLAHDVRI
CEEEEEEECEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEE
IRLPRHGASCPVGLGVSCSADRNIKCKINKDGIWIEKLDSNPGSLIPAELRQAGEGDVVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHCCCCCCEEE
IDLNRPMAEILKELTKYPVSTRLSLNGTIIVGRDIAHAKLKERLDRGEDLPQYIKDHPIY
EECCCCHHHHHHHHHCCCCEEEEEECEEEEEECCHHHHHHHHHHHCCCCCHHHHHHCCEE
YAGPAKTPAGMACGSMGPTTAGRMDSYVELFQSHGGSMVMLAKGNRSQQVTDACQKYGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCE
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFKQIKP
EEECCCCCEEEEECCCCCCEEEECCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHCCC
RCAK
CCCC
>Mature Secondary Structure 
ATPPFKYQPMFEKGKDTTEYYLLTKDYVSVSEFEGNPILKIEKEGLTAMANAAFRDVSF
CCCCCCCCCHHHCCCCCEEEEEEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHH
MLRRSHNEQVAKILSDPEASENDKYVALTFLRNAEVAAKGVLPFCQDTGTAIIHGEKGQQ
HHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHCCCCCCCCCCCEEEECCCCCE
VWTGYTDEEALSLGVYKTYTEENLRYSQNAPLNMYDEVNTKCNLPAQIDIEATEGMEYEF
EECCCCCHHHHEECEEEEECCCCCEECCCCCCCCHHCCCCCCCCCEEEEEEECCCCCEEE
LCVTKGGGSANKTYLYQETKAILNPGTLVPFLVEKMKTLGTAACPPYHIAFVIGGTSAEK
EEEECCCCCCCCEEEEEHHHHHCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
NLLTVKLASTHFYDNLPTTGNEFGRAFRDIELEKQVLEEAHKIGLGAQFGGKYLAHDVRI
CEEEEEEECEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCEEE
IRLPRHGASCPVGLGVSCSADRNIKCKINKDGIWIEKLDSNPGSLIPAELRQAGEGDVVK
EEECCCCCCCCCCCCEECCCCCCEEEEECCCCEEEEEECCCCCCCCCHHHHCCCCCCEEE
IDLNRPMAEILKELTKYPVSTRLSLNGTIIVGRDIAHAKLKERLDRGEDLPQYIKDHPIY
EECCCCHHHHHHHHHCCCCEEEEEECEEEEEECCHHHHHHHHHHHCCCCCHHHHHHCCEE
YAGPAKTPAGMACGSMGPTTAGRMDSYVELFQSHGGSMVMLAKGNRSQQVTDACQKYGGF
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCE
YLGSIGGPAAILAQNNIKSIECVEYPELGMEAIWKIEVEDFPAFILVDDKGNDFFKQIKP
EEECCCCCEEEEECCCCCCEEEECCCCCCCEEEEEEEECCCCEEEEEECCCCHHHHHCCC
RCAK
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]