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The map label for this gene is lacZ [H]

Identifier: 29347600

GI number: 29347600

Start: 2747782

End: 2748618

Strand: Reverse

Name: lacZ [H]

Synonym: BT_2190

Alternate gene names: 29347600

Gene position: 2748618-2747782 (Counterclockwise)

Preceding gene: 29347601

Following gene: 29347599

Centisome position: 43.91

GC content: 44.44

Gene sequence:

>837_bases
GTGGTGGAAGAGGAGAAGCGTTATTTGTTCTTCCGCCGTCCCGGTGTAGAAATCACCTTTGATAAGAAAGAAGGCAAGTT
GACCGGTATCCGTTATAATGGCGATAATATGCTTCATCTGCGTGAAGGATGGTCGCTGAATACGTTCCGTTTTATCAATA
ACGATGTGCGTAAATGGCAGGATACCCAAACGGAGGTAATCAGTTTCGACTGGAAATGGTCGGAAGATAATCAGTCGGCA
ATTGTAACGATTCAGTTGCAGGAAACCGTCGGAGATGTGAAAGTGCCTTATACATTGATTTACCGTTTGTATGGTAATGG
TGAAATAGATGTGGATGCTTCTTTTACAACGAATGATGACTTTAATCTTCCCCGTCTGTCGTTACAGGCCTTCTTTAATC
CTTCGTTGGAGCAGTTGGAATGGTATGGTCGCGGTCCGATAGAAAATTATCGGGATCGGAAGAATGCGGCTTATGTCGGC
AAATACCAGTCTGCTGTCAATGATATGAAAGAGAGTTATGCCCGTTCTCAGACGATGGGTGGGCGTTGTGATACCCGCTG
GCTGACTTTGACCAATAAAGCAGGTAAAGGAATCAAGATTACTGCTGCCGATACATTTGATTTCAGTGCTTTGCACTATA
CGGATAAGGACTTGTTTGAAATCAAGTACGGTCATGCCTTGCCTGATATTTATCGTGCGGAGGTGGTGCTGAATCTGGAT
TGCATACAGCGTGGTCTGGGTAATGCCAGTTGCGGACCGGGTCCCCGTCCCGCTTATGAAATTCAGAAGAATACAGTTTA
TAAATATGCTTTCCGCATGTCTCCTTTTTCAAAATAA

Upstream 100 bases:

>100_bases
ACATATCGTAGCTACGGAACAACTTTTGCTTCAAAAATCCCCGAATACCGGGCTATAGCCGGTAGCAGTTTCCGCCTCAT
CTAAAGAATCTCTGTTTAAG

Downstream 100 bases:

>100_bases
GGAAAGATTGACAGGATAACAAGGTCTTAATAGTTTGTAGAAAGAGTGAGCAGCCAAATCTTATAAAAGATGGCAACTGC
TTACTCTTTTTTTGTATTTA

Product: beta-galactosidase

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MVEEEKRYLFFRRPGVEITFDKKEGKLTGIRYNGDNMLHLREGWSLNTFRFINNDVRKWQDTQTEVISFDWKWSEDNQSA
IVTIQLQETVGDVKVPYTLIYRLYGNGEIDVDASFTTNDDFNLPRLSLQAFFNPSLEQLEWYGRGPIENYRDRKNAAYVG
KYQSAVNDMKESYARSQTMGGRCDTRWLTLTNKAGKGIKITAADTFDFSALHYTDKDLFEIKYGHALPDIYRAEVVLNLD
CIQRGLGNASCGPGPRPAYEIQKNTVYKYAFRMSPFSK

Sequences:

>Translated_278_residues
MVEEEKRYLFFRRPGVEITFDKKEGKLTGIRYNGDNMLHLREGWSLNTFRFINNDVRKWQDTQTEVISFDWKWSEDNQSA
IVTIQLQETVGDVKVPYTLIYRLYGNGEIDVDASFTTNDDFNLPRLSLQAFFNPSLEQLEWYGRGPIENYRDRKNAAYVG
KYQSAVNDMKESYARSQTMGGRCDTRWLTLTNKAGKGIKITAADTFDFSALHYTDKDLFEIKYGHALPDIYRAEVVLNLD
CIQRGLGNASCGPGPRPAYEIQKNTVYKYAFRMSPFSK
>Mature_278_residues
MVEEEKRYLFFRRPGVEITFDKKEGKLTGIRYNGDNMLHLREGWSLNTFRFINNDVRKWQDTQTEVISFDWKWSEDNQSA
IVTIQLQETVGDVKVPYTLIYRLYGNGEIDVDASFTTNDDFNLPRLSLQAFFNPSLEQLEWYGRGPIENYRDRKNAAYVG
KYQSAVNDMKESYARSQTMGGRCDTRWLTLTNKAGKGIKITAADTFDFSALHYTDKDLFEIKYGHALPDIYRAEVVLNLD
CIQRGLGNASCGPGPRPAYEIQKNTVYKYAFRMSPFSK

Specific function: The Wild-Type Enzyme Is An Ineffective Lactase. Two Classes Of Point Mutations Dramatically Improve Activity Of The Enzyme. [C]

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Escherichia coli, GI48994920, Length=288, Percent_Identity=27.7777777777778, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR011013
- InterPro:   IPR014718
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR004199
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02929 Bgal_small_N; PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 32202; Mature: 32202

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEEEKRYLFFRRPGVEITFDKKEGKLTGIRYNGDNMLHLREGWSLNTFRFINNDVRKWQ
CCCCCCEEEEEECCCCEEEEECCCCEEEEEEECCCCEEEEECCCCCEEEHHHHHHHHHHC
DTQTEVISFDWKWSEDNQSAIVTIQLQETVGDVKVPYTLIYRLYGNGEIDVDASFTTNDD
CCCEEEEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEEEEEECCCEEEEEEEEECCCC
FNLPRLSLQAFFNPSLEQLEWYGRGPIENYRDRKNAAYVGKYQSAVNDMKESYARSQTMG
CCCCEEEEEECCCCCHHHHHCCCCCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCC
GRCDTRWLTLTNKAGKGIKITAADTFDFSALHYTDKDLFEIKYGHALPDIYRAEVVLNLD
CCCCCEEEEEECCCCCCEEEEEECCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEEEHH
CIQRGLGNASCGPGPRPAYEIQKNTVYKYAFRMSPFSK
HHHHCCCCCCCCCCCCCCEEECCCEEEEEEEECCCCCC
>Mature Secondary Structure
MVEEEKRYLFFRRPGVEITFDKKEGKLTGIRYNGDNMLHLREGWSLNTFRFINNDVRKWQ
CCCCCCEEEEEECCCCEEEEECCCCEEEEEEECCCCEEEEECCCCCEEEHHHHHHHHHHC
DTQTEVISFDWKWSEDNQSAIVTIQLQETVGDVKVPYTLIYRLYGNGEIDVDASFTTNDD
CCCEEEEEEEEEECCCCCEEEEEEEEECCCCCCCCCEEEEEEEECCCEEEEEEEEECCCC
FNLPRLSLQAFFNPSLEQLEWYGRGPIENYRDRKNAAYVGKYQSAVNDMKESYARSQTMG
CCCCEEEEEECCCCCHHHHHCCCCCCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCC
GRCDTRWLTLTNKAGKGIKITAADTFDFSALHYTDKDLFEIKYGHALPDIYRAEVVLNLD
CCCCCEEEEEECCCCCCEEEEEECCCCCEEEEECCCCEEEEECCCCCCCCEEEEEEEEHH
CIQRGLGNASCGPGPRPAYEIQKNTVYKYAFRMSPFSK
HHHHCCCCCCCCCCCCCCEEECCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8088532; 10360571; 9741105 [H]