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The map label for this gene is rsmF [H]

Identifier: 29347452

GI number: 29347452

Start: 2564143

End: 2565621

Strand: Reverse

Name: rsmF [H]

Synonym: BT_2042

Alternate gene names: 29347452

Gene position: 2565621-2564143 (Counterclockwise)

Preceding gene: 29347455

Following gene: 29347444

Centisome position: 40.98

GC content: 49.56

Gene sequence:

>1479_bases
ATGAATTTACCCACTTCTTTTATCGACTATACCCGTGCCTTACTGGGCAATGAAGAATATGAAAAACTGGCTGCCGCCTT
GCAGCAGGAGCCTCCCGTCAGCATTCGGATCAATAAATTGAGAATGAAGGAGGAAGGATTAAGTTCGCTGACAGACAGTT
CTGCTCATTTTTCCTTCAACAAAGTTCCCTGGGCATCCGATGGCTACTATCTTGACGAGCGTCTGACTTTTACGTTCGAC
CCGTTGTTTCATGCCGGTTGCTACTATGTTCAGGAGGCTTCGTCCATGTTTGTGGAGCAGGTGCTGCGGCAGTATGTCGA
GTCTCCAGTGGTGATGCTCGATCTTTGTGCGGCGCCTGGAGGAAAATCAACCCATGCGCGTAGCGTGCTTTCGGAAGGGA
GTCTGCTGGTGGCGAATGAAGTGATCCGGAACCGTTCACAGGTGCTGGCGGAGAATCTGACGAAGTGGGGGCATCCGGAT
GTGGTGGTTACCAACAATGATCCGGCTGATTTCTCGGCACTTCCATCTTTCTTCGATGTCATTCTGACCGATGTGCCTTG
TTCGGGCGAGGGAATGTTTCGTAAAGACCCTGTGGCTGTGGAGGAATGGAGCCCGGAGAATGTAGAGATCTGCTGGCAGC
GTCAGCGGCGGATTATTGCGGATATTTGGCCCTGTCTGAAACCGGGCGGCATATTAATATATAGTACGTGTACCTATAAT
AGCAAAGAAGATGAAGAGAACGTCTGCTGGATTCAGCAGGAATTCGGTGCGGAGCTCTTGCCTCTGGAGGTGCGGAACGA
GTGGAATATAACAGGGAATTTACTCGATGGAGAGGATGAGTCTCAGGGAAGTTTGTCTGTTTGTCATTTTCTTCCTCATA
AGACAAAAGGCGAAGGATTCTTTCTGGCGGCACTTCGCAAACCGGATTCGGAAGATGAACCGGCAGCCTATTCATTCTCA
AAAGCAAAGTCGTCCAAGAAAAAAGATAAGAAAGGTGGAGCGGCTGCTTCTCCCGTTTCCAAAGAACACATGGGGATGGC
ACTGAACTGGCTGAAGCAGGAAAATGTCGAAAAATACACCCTGTCTGCGGAAGGCGCGGGCATCGTCGCATTTCCGCAAC
GATATACCGATGAACTGGCTGCCATGAAACAGCATCTGAAAGTGATACAGGCAGGCGTTCTGACTGGTGAAGTGAAAGGG
AGAGATTTGATTCCCGCTCATGCGTTGGCAATGAGCGCTACGCTCTTGCGGCAGGATGCTTTTGATACGGAAGAGGTGAG
TTATGAACAGGCTATCGCTTATTTACGAAAAGAGGCTATTACCTTGTCGGAAACAGCCCCTCGTGGTTATATCTTGCTTA
CTTACCGGAATATTCCTCTCGGATTTGTGAAGAATATAGGGAATCGTGCCAATAATCTTTATCCGCAGGAGTGGCGTATC
CGCAGCGGGTATTTGCCGGAGGAGATACGTACGCTGTAA

Upstream 100 bases:

>100_bases
TCTTTTATTTGTTTCTTTGTCTGTTAGACGTAGTTCGTATGTCAGAAAGTTGCAAAGAGACGAAATATTTTTGATATTTG
CAGGAATTAATAAATGAAAA

Downstream 100 bases:

>100_bases
AGATTATTTCCGAAAGAAACGGACAACGGATAGAATGCTTATCTTGATAGCTCGGCTAATTTATCATCCATAATTTAGGG
AAGTATTCCAGTGGGTATTT

Product: tRNA and rRNA cytosine-C5-methylase

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MNLPTSFIDYTRALLGNEEYEKLAAALQQEPPVSIRINKLRMKEEGLSSLTDSSAHFSFNKVPWASDGYYLDERLTFTFD
PLFHAGCYYVQEASSMFVEQVLRQYVESPVVMLDLCAAPGGKSTHARSVLSEGSLLVANEVIRNRSQVLAENLTKWGHPD
VVVTNNDPADFSALPSFFDVILTDVPCSGEGMFRKDPVAVEEWSPENVEICWQRQRRIIADIWPCLKPGGILIYSTCTYN
SKEDEENVCWIQQEFGAELLPLEVRNEWNITGNLLDGEDESQGSLSVCHFLPHKTKGEGFFLAALRKPDSEDEPAAYSFS
KAKSSKKKDKKGGAAASPVSKEHMGMALNWLKQENVEKYTLSAEGAGIVAFPQRYTDELAAMKQHLKVIQAGVLTGEVKG
RDLIPAHALAMSATLLRQDAFDTEEVSYEQAIAYLRKEAITLSETAPRGYILLTYRNIPLGFVKNIGNRANNLYPQEWRI
RSGYLPEEIRTL

Sequences:

>Translated_492_residues
MNLPTSFIDYTRALLGNEEYEKLAAALQQEPPVSIRINKLRMKEEGLSSLTDSSAHFSFNKVPWASDGYYLDERLTFTFD
PLFHAGCYYVQEASSMFVEQVLRQYVESPVVMLDLCAAPGGKSTHARSVLSEGSLLVANEVIRNRSQVLAENLTKWGHPD
VVVTNNDPADFSALPSFFDVILTDVPCSGEGMFRKDPVAVEEWSPENVEICWQRQRRIIADIWPCLKPGGILIYSTCTYN
SKEDEENVCWIQQEFGAELLPLEVRNEWNITGNLLDGEDESQGSLSVCHFLPHKTKGEGFFLAALRKPDSEDEPAAYSFS
KAKSSKKKDKKGGAAASPVSKEHMGMALNWLKQENVEKYTLSAEGAGIVAFPQRYTDELAAMKQHLKVIQAGVLTGEVKG
RDLIPAHALAMSATLLRQDAFDTEEVSYEQAIAYLRKEAITLSETAPRGYILLTYRNIPLGFVKNIGNRANNLYPQEWRI
RSGYLPEEIRTL
>Mature_492_residues
MNLPTSFIDYTRALLGNEEYEKLAAALQQEPPVSIRINKLRMKEEGLSSLTDSSAHFSFNKVPWASDGYYLDERLTFTFD
PLFHAGCYYVQEASSMFVEQVLRQYVESPVVMLDLCAAPGGKSTHARSVLSEGSLLVANEVIRNRSQVLAENLTKWGHPD
VVVTNNDPADFSALPSFFDVILTDVPCSGEGMFRKDPVAVEEWSPENVEICWQRQRRIIADIWPCLKPGGILIYSTCTYN
SKEDEENVCWIQQEFGAELLPLEVRNEWNITGNLLDGEDESQGSLSVCHFLPHKTKGEGFFLAALRKPDSEDEPAAYSFS
KAKSSKKKDKKGGAAASPVSKEHMGMALNWLKQENVEKYTLSAEGAGIVAFPQRYTDELAAMKQHLKVIQAGVLTGEVKG
RDLIPAHALAMSATLLRQDAFDTEEVSYEQAIAYLRKEAITLSETAPRGYILLTYRNIPLGFVKNIGNRANNLYPQEWRI
RSGYLPEEIRTL

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=284, Percent_Identity=27.112676056338, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI76150623, Length=284, Percent_Identity=27.112676056338, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI32698918, Length=182, Percent_Identity=32.967032967033, Blast_Score=86, Evalue=9e-17,
Organism=Homo sapiens, GI301336155, Length=266, Percent_Identity=28.9473684210526, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI39995082, Length=200, Percent_Identity=31.5, Blast_Score=80, Evalue=5e-15,
Organism=Escherichia coli, GI87081985, Length=495, Percent_Identity=28.8888888888889, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI2367212, Length=295, Percent_Identity=29.4915254237288, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17536757, Length=283, Percent_Identity=28.2685512367491, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI25143471, Length=266, Percent_Identity=29.3233082706767, Blast_Score=84, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324268, Length=310, Percent_Identity=26.7741935483871, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6319447, Length=385, Percent_Identity=26.7532467532467, Blast_Score=81, Evalue=4e-16,
Organism=Drosophila melanogaster, GI22024126, Length=284, Percent_Identity=27.112676056338, Blast_Score=115, Evalue=9e-26,
Organism=Drosophila melanogaster, GI21355201, Length=292, Percent_Identity=26.3698630136986, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI21356513, Length=217, Percent_Identity=30.4147465437788, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24668781, Length=217, Percent_Identity=30.4147465437788, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 55095; Mature: 55095

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLPTSFIDYTRALLGNEEYEKLAAALQQEPPVSIRINKLRMKEEGLSSLTDSSAHFSFN
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCCCEEEC
KVPWASDGYYLDERLTFTFDPLFHAGCYYVQEASSMFVEQVLRQYVESPVVMLDLCAAPG
CCCCCCCCEEECCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
GKSTHARSVLSEGSLLVANEVIRNRSQVLAENLTKWGHPDVVVTNNDPADFSALPSFFDV
CCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
ILTDVPCSGEGMFRKDPVAVEEWSPENVEICWQRQRRIIADIWPCLKPGGILIYSTCTYN
HHHCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
SKEDEENVCWIQQEFGAELLPLEVRNEWNITGNLLDGEDESQGSLSVCHFLPHKTKGEGF
CCCCCCCEEEEEHHCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEE
FLAALRKPDSEDEPAAYSFSKAKSSKKKDKKGGAAASPVSKEHMGMALNWLKQENVEKYT
EEEEECCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
LSAEGAGIVAFPQRYTDELAAMKQHLKVIQAGVLTGEVKGRDLIPAHALAMSATLLRQDA
EECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHCC
FDTEEVSYEQAIAYLRKEAITLSETAPRGYILLTYRNIPLGFVKNIGNRANNLYPQEWRI
CCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEE
RSGYLPEEIRTL
CCCCCCHHHHCC
>Mature Secondary Structure
MNLPTSFIDYTRALLGNEEYEKLAAALQQEPPVSIRINKLRMKEEGLSSLTDSSAHFSFN
CCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHCCCCCCEEEC
KVPWASDGYYLDERLTFTFDPLFHAGCYYVQEASSMFVEQVLRQYVESPVVMLDLCAAPG
CCCCCCCCEEECCEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
GKSTHARSVLSEGSLLVANEVIRNRSQVLAENLTKWGHPDVVVTNNDPADFSALPSFFDV
CCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHH
ILTDVPCSGEGMFRKDPVAVEEWSPENVEICWQRQRRIIADIWPCLKPGGILIYSTCTYN
HHHCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCC
SKEDEENVCWIQQEFGAELLPLEVRNEWNITGNLLDGEDESQGSLSVCHFLPHKTKGEGF
CCCCCCCEEEEEHHCCCEEEEEEECCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCEE
FLAALRKPDSEDEPAAYSFSKAKSSKKKDKKGGAAASPVSKEHMGMALNWLKQENVEKYT
EEEEECCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEEE
LSAEGAGIVAFPQRYTDELAAMKQHLKVIQAGVLTGEVKGRDLIPAHALAMSATLLRQDA
EECCCCEEEECCHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHCC
FDTEEVSYEQAIAYLRKEAITLSETAPRGYILLTYRNIPLGFVKNIGNRANNLYPQEWRI
CCHHHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCEEE
RSGYLPEEIRTL
CCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA