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The map label for this gene is cimA [H]

Identifier: 29347268

GI number: 29347268

Start: 2331081

End: 2332580

Strand: Reverse

Name: cimA [H]

Synonym: BT_1858

Alternate gene names: 29347268

Gene position: 2332580-2331081 (Counterclockwise)

Preceding gene: 29347269

Following gene: 29347267

Centisome position: 37.26

GC content: 45.6

Gene sequence:

>1500_bases
ATGGACACAACGCTCCGTGACGGTGAACAAACCAGCGGAGTATCTTTTGTGCCCCATGAGAAACTAATGATTGCCCGTTT
ACTATTGGAGGATTTGAAGGTAGACCGGGTAGAGGTTGCTTCAGCACGTGTATCGGAAGGTGAGTTTGAAGCCGTGAAGA
TGATTTGTGATTGGGCAGCCCGCCGCAATCTGCTTCAGAAAGTGGAAGTGTTGGGATTTGTGGACGGTCATACTTCGGTG
GACTGGATACAACGTACCGGGTGCCGTGTCATCAATCTTCTTTGTAAGGGGTCATTGAAACATTGCACCCAACAATTGAA
AAAGACTCCCGAAGAACATATCGCAGATATTATCAACGTAGTACATTACGCAGATGAACAGGATATCGGAGTGAATGTAT
ATCTGGAAGACTGGAGTAACGGAATGAAAGATTCTCCCGAATATGTGTTTCAGTTGATGGATGGCTTGAAGCAGACAAGC
ATCAGACGTTATATGTTGCCCGATACATTGGGAATCCTGAACCCGCTGCAGGTGATAGAATATATGCGGAAGATGAAGAA
GCGTTATCCGAATACCCATTTTGATTTTCACGCGCATAATGATTATGATCTGGCGGTAAGTAATGTGCTGGCTGCCGTGT
TGAGCGGTGTCAGAGGACTGCATACTACGATTAACGGACTTGGCGAACGGGCCGGTAATGCACCCCTTTCCAGTGTGCAG
GCTATTCTGAAAGATCATTTCAATGCAATGACCAATATTGACGAAAGCCGCCTGAATGATGTCAGTCGGGTGGTAGAGTC
CTATTCCGGTATTGTGATTCCTGCCAATAAACCGATTGTGGGAGAGAATGTCTTTACACAAGTTGCAGGCGTTCACGCCG
ACGGCGATAATAAGAATAATTTGTATTGTAATGATTTACTTCCCGAACGTTTCGGACGTAAGCGTGAGTATGCTTTAGGA
AAAACAAGTGGAAAGGCAAATATCCGTAAGAATCTCGAAGATCTGGGACTGGAACTCGATGAAGATGCCATGCGCAAGGT
AACGGAGCGAATCATTGAACTGGGGGACAAGAAAGAATTGGTGACTCAGGAGGATTTGCCGTATATTGTATCGGATGTAT
TGAAACACGGGGCGATAGGCGAGAAGGTCAAGCTGAAGAGTTATTTTGTAAATCTGGCTCACGGTCTGAAACCGATGGCG
ACTTTAAAGATCGAAATAAACGGAAAGGAATACGAAGAAAGTTCAAGTGGCGATGGTCAGTATGATGCTTTCGTACGTGC
GTTGCGCAAAATTTATAAAGTGACGTTAGGACGTAAATTCCCCATGTTGACGAATTATGCGGTATCTATCCCACCGGGTG
GGCGTACGGATGCATTTGTGCAGACAGTAATTACCTGGAACTATGACGAACAGGTGTTCCGTACCCGTGGACTTGATGCC
GACCAGACGGAGGCTGCCATCAAAGCAACAATGAAGATGTTGAATCTACTTGAAGAATAA

Upstream 100 bases:

>100_bases
AGAAACTTTGTCTGATGAACGGACTGGATGACATAGACTTCTTGCTAAGCAATAAAAATAAAATAGAAGAATGGGAAAAC
AAGGCGTCAAAATAGAGATC

Downstream 100 bases:

>100_bases
GTTGATTTTATATTAACATGCATTTGATGCTTCCGATATGACAGAGATTAATTAACTTTGAGTAGTATAAAAGATAAAAA
GAAAAGTAACATGGATTTTA

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 499; Mature: 499

Protein sequence:

>499_residues
MDTTLRDGEQTSGVSFVPHEKLMIARLLLEDLKVDRVEVASARVSEGEFEAVKMICDWAARRNLLQKVEVLGFVDGHTSV
DWIQRTGCRVINLLCKGSLKHCTQQLKKTPEEHIADIINVVHYADEQDIGVNVYLEDWSNGMKDSPEYVFQLMDGLKQTS
IRRYMLPDTLGILNPLQVIEYMRKMKKRYPNTHFDFHAHNDYDLAVSNVLAAVLSGVRGLHTTINGLGERAGNAPLSSVQ
AILKDHFNAMTNIDESRLNDVSRVVESYSGIVIPANKPIVGENVFTQVAGVHADGDNKNNLYCNDLLPERFGRKREYALG
KTSGKANIRKNLEDLGLELDEDAMRKVTERIIELGDKKELVTQEDLPYIVSDVLKHGAIGEKVKLKSYFVNLAHGLKPMA
TLKIEINGKEYEESSSGDGQYDAFVRALRKIYKVTLGRKFPMLTNYAVSIPPGGRTDAFVQTVITWNYDEQVFRTRGLDA
DQTEAAIKATMKMLNLLEE

Sequences:

>Translated_499_residues
MDTTLRDGEQTSGVSFVPHEKLMIARLLLEDLKVDRVEVASARVSEGEFEAVKMICDWAARRNLLQKVEVLGFVDGHTSV
DWIQRTGCRVINLLCKGSLKHCTQQLKKTPEEHIADIINVVHYADEQDIGVNVYLEDWSNGMKDSPEYVFQLMDGLKQTS
IRRYMLPDTLGILNPLQVIEYMRKMKKRYPNTHFDFHAHNDYDLAVSNVLAAVLSGVRGLHTTINGLGERAGNAPLSSVQ
AILKDHFNAMTNIDESRLNDVSRVVESYSGIVIPANKPIVGENVFTQVAGVHADGDNKNNLYCNDLLPERFGRKREYALG
KTSGKANIRKNLEDLGLELDEDAMRKVTERIIELGDKKELVTQEDLPYIVSDVLKHGAIGEKVKLKSYFVNLAHGLKPMA
TLKIEINGKEYEESSSGDGQYDAFVRALRKIYKVTLGRKFPMLTNYAVSIPPGGRTDAFVQTVITWNYDEQVFRTRGLDA
DQTEAAIKATMKMLNLLEE
>Mature_499_residues
MDTTLRDGEQTSGVSFVPHEKLMIARLLLEDLKVDRVEVASARVSEGEFEAVKMICDWAARRNLLQKVEVLGFVDGHTSV
DWIQRTGCRVINLLCKGSLKHCTQQLKKTPEEHIADIINVVHYADEQDIGVNVYLEDWSNGMKDSPEYVFQLMDGLKQTS
IRRYMLPDTLGILNPLQVIEYMRKMKKRYPNTHFDFHAHNDYDLAVSNVLAAVLSGVRGLHTTINGLGERAGNAPLSSVQ
AILKDHFNAMTNIDESRLNDVSRVVESYSGIVIPANKPIVGENVFTQVAGVHADGDNKNNLYCNDLLPERFGRKREYALG
KTSGKANIRKNLEDLGLELDEDAMRKVTERIIELGDKKELVTQEDLPYIVSDVLKHGAIGEKVKLKSYFVNLAHGLKPMA
TLKIEINGKEYEESSSGDGQYDAFVRALRKIYKVTLGRKFPMLTNYAVSIPPGGRTDAFVQTVITWNYDEQVFRTRGLDA
DQTEAAIKATMKMLNLLEE

Specific function: Catalyzes the condensation of pyruvate and acetyl- coenzyme A to form (R)-citramalate [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=505, Percent_Identity=29.5049504950495, Blast_Score=164, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6320071, Length=379, Percent_Identity=27.4406332453826, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6320019, Length=367, Percent_Identity=27.5204359673025, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR011830
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.1.182 [H]

Molecular weight: Translated: 56120; Mature: 56120

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTTLRDGEQTSGVSFVPHEKLMIARLLLEDLKVDRVEVASARVSEGEFEAVKMICDWAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHH
RRNLLQKVEVLGFVDGHTSVDWIQRTGCRVINLLCKGSLKHCTQQLKKTPEEHIADIINV
HHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHH
VHYADEQDIGVNVYLEDWSNGMKDSPEYVFQLMDGLKQTSIRRYMLPDTLGILNPLQVIE
HHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHH
YMRKMKKRYPNTHFDFHAHNDYDLAVSNVLAAVLSGVRGLHTTINGLGERAGNAPLSSVQ
HHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AILKDHFNAMTNIDESRLNDVSRVVESYSGIVIPANKPIVGENVFTQVAGVHADGDNKNN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHCEECCCCCCCC
LYCNDLLPERFGRKREYALGKTSGKANIRKNLEDLGLELDEDAMRKVTERIIELGDKKEL
EEECCCCHHHHCCCHHHHCCCCCCCHHHHHCHHHCCCCCCHHHHHHHHHHHHHCCCCHHH
VTQEDLPYIVSDVLKHGAIGEKVKLKSYFVNLAHGLKPMATLKIEINGKEYEESSSGDGQ
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCH
YDAFVRALRKIYKVTLGRKFPMLTNYAVSIPPGGRTDAFVQTVITWNYDEQVFRTRGLDA
HHHHHHHHHHHHHHHHCCCCCCHHCCEEECCCCCCHHHHHHHHHEECCHHHHHHHCCCCC
DQTEAAIKATMKMLNLLEE
CHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDTTLRDGEQTSGVSFVPHEKLMIARLLLEDLKVDRVEVASARVSEGEFEAVKMICDWAA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHHH
RRNLLQKVEVLGFVDGHTSVDWIQRTGCRVINLLCKGSLKHCTQQLKKTPEEHIADIINV
HHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHH
VHYADEQDIGVNVYLEDWSNGMKDSPEYVFQLMDGLKQTSIRRYMLPDTLGILNPLQVIE
HHHCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHH
YMRKMKKRYPNTHFDFHAHNDYDLAVSNVLAAVLSGVRGLHTTINGLGERAGNAPLSSVQ
HHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
AILKDHFNAMTNIDESRLNDVSRVVESYSGIVIPANKPIVGENVFTQVAGVHADGDNKNN
HHHHHHHHHHHCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHHHHCEECCCCCCCC
LYCNDLLPERFGRKREYALGKTSGKANIRKNLEDLGLELDEDAMRKVTERIIELGDKKEL
EEECCCCHHHHCCCHHHHCCCCCCCHHHHHCHHHCCCCCCHHHHHHHHHHHHHCCCCHHH
VTQEDLPYIVSDVLKHGAIGEKVKLKSYFVNLAHGLKPMATLKIEINGKEYEESSSGDGQ
CCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCCCCH
YDAFVRALRKIYKVTLGRKFPMLTNYAVSIPPGGRTDAFVQTVITWNYDEQVFRTRGLDA
HHHHHHHHHHHHHHHHCCCCCCHHCCEEECCCCCCHHHHHHHHHEECCHHHHHHHCCCCC
DQTEAAIKATMKMLNLLEE
CHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]