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The map label for this gene is leuB [H]

Identifier: 29347267

GI number: 29347267

Start: 2329929

End: 2330990

Strand: Reverse

Name: leuB [H]

Synonym: BT_1857

Alternate gene names: 29347267

Gene position: 2330990-2329929 (Counterclockwise)

Preceding gene: 29347268

Following gene: 29347266

Centisome position: 37.23

GC content: 45.2

Gene sequence:

>1062_bases
ATGGATTTTAAAATTGCTGTATTAGCAGGCGACGGTATCGGACCGGAGATTTCGGTACAAGGTGTAGATGTAATGAGTGC
CGTTTGCGAGAAGTTTGGCCACAAAGTAAGTTACGAATATGCAATCTGCGGTGCAGATGCGATTGATAAAGTAGGTGATC
CGTTCCCGGAAGAAACATATGAGGTTTGCAAGAATGCAGATGCTGTTTTGTTTTCGGCCGTAGGTGATCCGAAGTTTGAT
AATGACCCTACTGCGAAAGTTCGTCCGGAGCAGGGATTGCTGGCAATGCGTAAGAAATTGGGATTGTTTGCTAATATTCG
TCCGGTACAGACATTCAAATGCCTGATCCATAAATCTCCGTTGCGTGCGGAACTGGTTGAGAATGCGGATTTCATCTGCA
TCCGTGAGCTGACCGGCGGTATGTACTTCGGTGAGAAATATCAGGATAATGATAAAGCATACGATACAAACTACTACACT
CGTCCGGAAATTGAACGTATCCTGAAAGTTGCTTTTGAATATGCAATGAAACGCAGAAAGCACCTGACTGTGGTAGACAA
GGCAAATGTTCTGGCTTCTTCCCGTCTGTGGCGTCAGATTGCACAGGAAATGGCTCCCAATTATCCGGAGGTGACAACTG
ACTATATGTTTGTGGATAATGCTGCCATGAAGATGATCCAAGAACCAGCTTTCTTTGACGTGATGGTGACTGAAAATACA
TTCGGGGATATTCTGACTGACGAAGGTTCTGTTATCAGCGGCTCTATGGGATTGCTTCCTTCAGCTTCTACCGGTGAAAG
TACTCCAGTATTCGAACCGATTCATGGTTCATGGCCACAGGCGAAGGGCTTGAATATTGCGAACCCGTTGGCACAGATTC
TGTCTGTAGCTATGTTATTTGAATACTTCGACTGCAAGGAAGAAGGTGCGCTGATTCGTAAGGCGGTTGATGCATCACTG
GATGAAAACGTACGTACACCTGAAATTCAAGTGGCCGATGGTGCCAAATACGGTACAAAGGAAGTAGGACAGTGGATTGT
TGACTATATCAAGAAGGCTTAA

Upstream 100 bases:

>100_bases
TGAAGAATAAGTTGATTTTATATTAACATGCATTTGATGCTTCCGATATGACAGAGATTAATTAACTTTGAGTAGTATAA
AAGATAAAAAGAAAAGTAAC

Downstream 100 bases:

>100_bases
ACAGATATTCTGAATAAGATATATGGAGGCTGGATTCTTTAAATGGAGTTCAGCCTCTTTTTGTTCATTCCGTTTCTTTG
TTTTTTTACTGAAGAAAATG

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MDFKIAVLAGDGIGPEISVQGVDVMSAVCEKFGHKVSYEYAICGADAIDKVGDPFPEETYEVCKNADAVLFSAVGDPKFD
NDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLIHKSPLRAELVENADFICIRELTGGMYFGEKYQDNDKAYDTNYYT
RPEIERILKVAFEYAMKRRKHLTVVDKANVLASSRLWRQIAQEMAPNYPEVTTDYMFVDNAAMKMIQEPAFFDVMVTENT
FGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLFEYFDCKEEGALIRKAVDASL
DENVRTPEIQVADGAKYGTKEVGQWIVDYIKKA

Sequences:

>Translated_353_residues
MDFKIAVLAGDGIGPEISVQGVDVMSAVCEKFGHKVSYEYAICGADAIDKVGDPFPEETYEVCKNADAVLFSAVGDPKFD
NDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLIHKSPLRAELVENADFICIRELTGGMYFGEKYQDNDKAYDTNYYT
RPEIERILKVAFEYAMKRRKHLTVVDKANVLASSRLWRQIAQEMAPNYPEVTTDYMFVDNAAMKMIQEPAFFDVMVTENT
FGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLFEYFDCKEEGALIRKAVDASL
DENVRTPEIQVADGAKYGTKEVGQWIVDYIKKA
>Mature_353_residues
MDFKIAVLAGDGIGPEISVQGVDVMSAVCEKFGHKVSYEYAICGADAIDKVGDPFPEETYEVCKNADAVLFSAVGDPKFD
NDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLIHKSPLRAELVENADFICIRELTGGMYFGEKYQDNDKAYDTNYYT
RPEIERILKVAFEYAMKRRKHLTVVDKANVLASSRLWRQIAQEMAPNYPEVTTDYMFVDNAAMKMIQEPAFFDVMVTENT
FGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLFEYFDCKEEGALIRKAVDASL
DENVRTPEIQVADGAKYGTKEVGQWIVDYIKKA

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=324, Percent_Identity=30.2469135802469, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI28178816, Length=321, Percent_Identity=27.7258566978193, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI28178821, Length=321, Percent_Identity=27.7258566978193, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI4758582, Length=354, Percent_Identity=27.4011299435028, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI28178838, Length=335, Percent_Identity=27.7611940298507, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI28178819, Length=159, Percent_Identity=34.5911949685535, Blast_Score=99, Evalue=7e-21,
Organism=Escherichia coli, GI87081683, Length=361, Percent_Identity=45.4293628808864, Blast_Score=311, Evalue=3e-86,
Organism=Escherichia coli, GI1788101, Length=319, Percent_Identity=35.1097178683386, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1787381, Length=384, Percent_Identity=23.1770833333333, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI71986051, Length=330, Percent_Identity=30, Blast_Score=142, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17550882, Length=342, Percent_Identity=28.9473684210526, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=358, Percent_Identity=27.0949720670391, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17505779, Length=334, Percent_Identity=28.7425149700599, Blast_Score=114, Evalue=7e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=365, Percent_Identity=40.8219178082192, Blast_Score=261, Evalue=2e-70,
Organism=Saccharomyces cerevisiae, GI6322097, Length=359, Percent_Identity=32.5905292479109, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6324709, Length=366, Percent_Identity=30.327868852459, Blast_Score=135, Evalue=9e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=331, Percent_Identity=29.607250755287, Blast_Score=128, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24643268, Length=323, Percent_Identity=30.030959752322, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24643270, Length=323, Percent_Identity=30.030959752322, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24661184, Length=332, Percent_Identity=27.4096385542169, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI281362242, Length=348, Percent_Identity=27.8735632183908, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24648872, Length=348, Percent_Identity=27.8735632183908, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI161078639, Length=241, Percent_Identity=28.2157676348548, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161078637, Length=334, Percent_Identity=26.3473053892216, Blast_Score=102, Evalue=3e-22,
Organism=Drosophila melanogaster, GI161078633, Length=334, Percent_Identity=26.3473053892216, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24650122, Length=334, Percent_Identity=26.3473053892216, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI161078635, Length=334, Percent_Identity=26.3473053892216, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI20130355, Length=230, Percent_Identity=25.2173913043478, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39179; Mature: 39179

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKIAVLAGDGIGPEISVQGVDVMSAVCEKFGHKVSYEYAICGADAIDKVGDPFPEETY
CCEEEEEEECCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCCHHHH
EVCKNADAVLFSAVGDPKFDNDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLIHKSP
HHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCHHCCCCHHHHHHHHHHCCC
LRAELVENADFICIRELTGGMYFGEKYQDNDKAYDTNYYTRPEIERILKVAFEYAMKRRK
HHHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HLTVVDKANVLASSRLWRQIAQEMAPNYPEVTTDYMFVDNAAMKMIQEPAFFDVMVTENT
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCCCEEEEEEECCC
FGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLF
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EYFDCKEEGALIRKAVDASLDENVRTPEIQVADGAKYGTKEVGQWIVDYIKKA
HHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDFKIAVLAGDGIGPEISVQGVDVMSAVCEKFGHKVSYEYAICGADAIDKVGDPFPEETY
CCEEEEEEECCCCCCCEEECCHHHHHHHHHHHCCCCEEEEEEECCHHHHHCCCCCCHHHH
EVCKNADAVLFSAVGDPKFDNDPTAKVRPEQGLLAMRKKLGLFANIRPVQTFKCLIHKSP
HHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCHHCCCCHHHHHHHHHHCCC
LRAELVENADFICIRELTGGMYFGEKYQDNDKAYDTNYYTRPEIERILKVAFEYAMKRRK
HHHHHHCCCCEEEEEECCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
HLTVVDKANVLASSRLWRQIAQEMAPNYPEVTTDYMFVDNAAMKMIQEPAFFDVMVTENT
CEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHCCCCEEEEEEECCC
FGDILTDEGSVISGSMGLLPSASTGESTPVFEPIHGSWPQAKGLNIANPLAQILSVAMLF
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
EYFDCKEEGALIRKAVDASLDENVRTPEIQVADGAKYGTKEVGQWIVDYIKKA
HHHCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA