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The map label for this gene is queF [H]

Identifier: 29346974

GI number: 29346974

Start: 1929521

End: 1929976

Strand: Reverse

Name: queF [H]

Synonym: BT_1564

Alternate gene names: 29346974

Gene position: 1929976-1929521 (Counterclockwise)

Preceding gene: 29346976

Following gene: 29346973

Centisome position: 30.83

GC content: 41.89

Gene sequence:

>456_bases
ATGGCTGAATTAAAAGACCAACTTTCCTTATTAGGAAGAAAAACGGAGTATAAACAAGACTATGCTCCTGAAGTATTGGA
GGCTTTCGATAATAAGCATCCGGAAAATGACTACTGGGTACGTTTCAACTGTCCCGAATTTACAAGCCTGTGCCCCATAA
CCGGACAACCTGACTTTGCAGAAATACGTATCAGCTACATCCCCGACATTAAGATGGTGGAAAGCAAGAGTTTGAAACTT
TATCTGTTCAGCTTCCGTAATCATGGTGCATTTCATGAAGACTGTGTGAACATCATCATGAAAGATTTGATAAAACTGAT
GAATCCCAAATATATCGAGGTTACGGGTATCTTTACTCCACGTGGCGGTATTTCGATTTATCCGTATGCCAATTATGGCC
GTCCGGGAACGAAGTTCGAACAGATGGCAGAGCACCGGTTGATGAATCGGGAATAA

Upstream 100 bases:

>100_bases
TGAACCATCGATGGATAAGCCGTTTCAGAGAATATGGTGACAGGTGAAAGATGTTTTGCGTTTTTTTCTATATGAAGAGA
GTTTAAAACTATATTAAACA

Downstream 100 bases:

>100_bases
CCAGATATAAAAATAATAATACGAGCAGAATATGACTCATAATATACCTACTGAATTGCAGAGATACGTGCAGGAAAGGA
TTATCCCGCAATATGCCGGC

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 151; Mature: 150

Protein sequence:

>151_residues
MAELKDQLSLLGRKTEYKQDYAPEVLEAFDNKHPENDYWVRFNCPEFTSLCPITGQPDFAEIRISYIPDIKMVESKSLKL
YLFSFRNHGAFHEDCVNIIMKDLIKLMNPKYIEVTGIFTPRGGISIYPYANYGRPGTKFEQMAEHRLMNRE

Sequences:

>Translated_151_residues
MAELKDQLSLLGRKTEYKQDYAPEVLEAFDNKHPENDYWVRFNCPEFTSLCPITGQPDFAEIRISYIPDIKMVESKSLKL
YLFSFRNHGAFHEDCVNIIMKDLIKLMNPKYIEVTGIFTPRGGISIYPYANYGRPGTKFEQMAEHRLMNRE
>Mature_150_residues
AELKDQLSLLGRKTEYKQDYAPEVLEAFDNKHPENDYWVRFNCPEFTSLCPITGQPDFAEIRISYIPDIKMVESKSLKLY
LFSFRNHGAFHEDCVNIIMKDLIKLMNPKYIEVTGIFTPRGGISIYPYANYGRPGTKFEQMAEHRLMNRE

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=81, Percent_Identity=39.5061728395062, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016856
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 17680; Mature: 17548

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELKDQLSLLGRKTEYKQDYAPEVLEAFDNKHPENDYWVRFNCPEFTSLCPITGQPDFA
CCCHHHHHHHHCCCCHHCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
EIRISYIPDIKMVESKSLKLYLFSFRNHGAFHEDCVNIIMKDLIKLMNPKYIEVTGIFTP
EEEEEECCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECC
RGGISIYPYANYGRPGTKFEQMAEHRLMNRE
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
AELKDQLSLLGRKTEYKQDYAPEVLEAFDNKHPENDYWVRFNCPEFTSLCPITGQPDFA
CCHHHHHHHHCCCCHHCCCCCHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
EIRISYIPDIKMVESKSLKLYLFSFRNHGAFHEDCVNIIMKDLIKLMNPKYIEVTGIFTP
EEEEEECCCEEEECCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECC
RGGISIYPYANYGRPGTKFEQMAEHRLMNRE
CCCEEEEECCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA