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The map label for this gene is hisF

Identifier: 29346788

GI number: 29346788

Start: 1706167

End: 1706922

Strand: Reverse

Name: hisF

Synonym: BT_1378

Alternate gene names: 29346788

Gene position: 1706922-1706167 (Counterclockwise)

Preceding gene: 29346789

Following gene: 29346787

Centisome position: 27.27

GC content: 48.68

Gene sequence:

>756_bases
GTGTTAGCAAAAAGAATCATACCCTGTCTGGATATAAAAGACGGACAGACCGTAAAAGGAACGAACTTTGTCAATTTGCG
CCAGGCCGGTGATCCGGTAGAACTGGGGCGGGCTTACAGCGAACAAGGAGCAGATGAACTTGTTTTTTTGGATATTACTG
CAAGTCACGAAGGACGTAAGACTTTCACGGAACTGGTAAAGCGGATTGCTGCCAATATCAATATCCCGTTTACCGTGGGC
GGTGGCATCAATGAATTGAGCGATGTAGATCGCTTGCTGAATGCCGGAGCGGATAAGATCTCAATCAATTCTTCCGCCAT
TCGCAACCCTCAGCTGATTGATGATATAGCCAAGAACTTCGGCTCTCAGGTTTGCGTTCTGGCGGTAGATGCCAAGCAAA
CAGAAAACGGCTGGAAGTGTTATCTGAACGGCGGGCGTATCGAAACCGATAAAGAATTGCTGGCATGGACTAAAGAGGCT
CAGGAGCGTGGAGCAGGCGAAATCCTGTTTACGAGCATGAACCATGATGGGGTGAAGACCGGTTATGCGAACGAGGCGCT
TGCTTCCTTGGCCGATCAACTCTCTATTCCCGTGATCGCTTCGGGCGGGGCAGGGCTGAAAGAACACTTCCGTGATGCTT
TTTTAGTAGGAAAAGCAGATGCGGCGTTGGCTGCCAGCGTTTTTCATTTCGGAGAAATTAAAATTCCCGAATTAAAATCG
TATCTTTGCGGCGAAGGAATAACCATCAGAGGGTGA

Upstream 100 bases:

>100_bases
GCTAAAATGAAATGGAGCGCCCTGTTATAATGAAAAAATAAAGAAGCAGGTTCTCGGATTAGTAGAATCTAAATTGTCAA
ATAGTAAATAAGATAGAATT

Downstream 100 bases:

>100_bases
GGCGGCATGGGCTCACACAAACGGTATAGTAAATTGAAATAGTAATTAGAACTTAATATAAGATGGAATTGGATTTCGAT
AAGATGAACGGACTTGTTCC

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRAYSEQGADELVFLDITASHEGRKTFTELVKRIAANINIPFTVG
GGINELSDVDRLLNAGADKISINSSAIRNPQLIDDIAKNFGSQVCVLAVDAKQTENGWKCYLNGGRIETDKELLAWTKEA
QERGAGEILFTSMNHDGVKTGYANEALASLADQLSIPVIASGGAGLKEHFRDAFLVGKADAALAASVFHFGEIKIPELKS
YLCGEGITIRG

Sequences:

>Translated_251_residues
MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRAYSEQGADELVFLDITASHEGRKTFTELVKRIAANINIPFTVG
GGINELSDVDRLLNAGADKISINSSAIRNPQLIDDIAKNFGSQVCVLAVDAKQTENGWKCYLNGGRIETDKELLAWTKEA
QERGAGEILFTSMNHDGVKTGYANEALASLADQLSIPVIASGGAGLKEHFRDAFLVGKADAALAASVFHFGEIKIPELKS
YLCGEGITIRG
>Mature_251_residues
MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRAYSEQGADELVFLDITASHEGRKTFTELVKRIAANINIPFTVG
GGINELSDVDRLLNAGADKISINSSAIRNPQLIDDIAKNFGSQVCVLAVDAKQTENGWKCYLNGGRIETDKELLAWTKEA
QERGAGEILFTSMNHDGVKTGYANEALASLADQLSIPVIASGGAGLKEHFRDAFLVGKADAALAASVFHFGEIKIPELKS
YLCGEGITIRG

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=49.8054474708171, Blast_Score=234, Evalue=5e-63,
Organism=Escherichia coli, GI87082028, Length=180, Percent_Identity=30, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=313, Percent_Identity=33.5463258785943, Blast_Score=158, Evalue=8e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_BACTN (Q8A7Z6)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_810291.1
- ProteinModelPortal:   Q8A7Z6
- SMR:   Q8A7Z6
- GeneID:   1072960
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_1378
- NMPDR:   fig|226186.1.peg.1378
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q8A7Z6
- ProtClustDB:   PRK02083
- BioCyc:   BTHE226186:BT_1378-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26934; Mature: 26934

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRAYSEQGADELVFLDITASHEGRK
CCHHHCCCCEECCCCCEECCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHH
TFTELVKRIAANINIPFTVGGGINELSDVDRLLNAGADKISINSSAIRNPQLIDDIAKNF
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHHHHHHC
GSQVCVLAVDAKQTENGWKCYLNGGRIETDKELLAWTKEAQERGAGEILFTSMNHDGVKT
CCCEEEEEEECCCCCCCCEEEEECCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYANEALASLADQLSIPVIASGGAGLKEHFRDAFLVGKADAALAASVFHFGEIKIPELKS
CCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHEECCCHHHHHHHHHHCCCEECHHHHH
YLCGEGITIRG
HHCCCCCEECC
>Mature Secondary Structure
MLAKRIIPCLDIKDGQTVKGTNFVNLRQAGDPVELGRAYSEQGADELVFLDITASHEGRK
CCHHHCCCCEECCCCCEECCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEEEECCCCCHH
TFTELVKRIAANINIPFTVGGGINELSDVDRLLNAGADKISINSSAIRNPQLIDDIAKNF
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCEEEECHHHCCCCHHHHHHHHHC
GSQVCVLAVDAKQTENGWKCYLNGGRIETDKELLAWTKEAQERGAGEILFTSMNHDGVKT
CCCEEEEEEECCCCCCCCEEEEECCEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCC
GYANEALASLADQLSIPVIASGGAGLKEHFRDAFLVGKADAALAASVFHFGEIKIPELKS
CCHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHEECCCHHHHHHHHHHCCCEECHHHHH
YLCGEGITIRG
HHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928