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The map label for this gene is zraS [H]

Identifier: 29346265

GI number: 29346265

Start: 1043365

End: 1044642

Strand: Reverse

Name: zraS [H]

Synonym: BT_0855

Alternate gene names: 29346265

Gene position: 1044642-1043365 (Counterclockwise)

Preceding gene: 29346266

Following gene: 29346263

Centisome position: 16.69

GC content: 39.67

Gene sequence:

>1278_bases
ATGAAACGATTTGCATTCAGAGTAATTTTACATATTTTACTGATAGTATTGTTTTCTATCGGCAGCTACCTGCTATTTCA
AAAGCAGTTATGGTTCAGTACTACCATCTGCCTTATATTACTGATAACCATTGGAATACATCTTTACCGTATGCAGTTCA
AACAAATTGCTCTGTTGCGACGATTGACAGACGGCCTCCGCTATAATGACATGATGCAAACTTTCCATCCACCTTTCAAT
AACAAAATAATGAATGAATGGGCGGAAGAACTTTCGGATACTTTAAAAGATTTTCGGGGAAGATTGCTGGCAGAAGAGAT
CAAGCACCAATACTACGAGAACTTATTGAACAAAGTTGATACGGCAGTGCTTGTTGCCGATAAAGCCGGACATATAGAAT
GGATGAATCAGGCGGCCGTTACCCACTTAGGACAAATATCACAATTGCCGGAAACATTGCTGAAAGCTTCTGTTGCCCAT
GACACCCCCGTCATCCGTATCGAACAAAATAGCACTGTGCTGGAAATGGCCATATCACGCACTACATTTGCCACTCAAGG
TAGAGAACAGCAGTTAATCAGTTTAAAAAACATTCATTCTGTATTGGAACGCAATGAAATGGAAGCCTGGCAAAAGCTGA
TACGCGTGCTGACTCATGAAATCATGAACTCTATCACACCAATCATTTCTCTTTCGGAAACATTAAGCGAGAGAGGAATC
CCCAGTCAACTGGGAGAAAAAGAATATTCTGTTATGCTGCAAGCCATGCAGACCATTCACAGAAGAAGCAAAGGATTACT
GGAATTTGTAGAGAACTATCGTCGGCTCACACGAATCCCTGCTCCTATTCGTACACAAATTTCTATTGCGGAATTATTCA
CGGATTTAAAGAAATTATTTCCGGAAGAAGAGTTTCAGTTTGAGGTTCCCTCACCCGAACTGAAATTAAATGTGGACCGA
ACACAAATAGAACAGATTCTTATTAATCTGCTGAAAAATGCCCGTGAAGCATGCAGTCGAAAATCAGATAAGAAGATTCA
GGTAAAAGCAAGAAAACTTTCTGCCGGAAATACGACATTAACGATATCTGATAATGGAGAAGGTATTCTGCCCGATGTGC
TGGATAAAATATTTGTTCCTTTCTTTACCACCAAAACATCCGGTTCGGGTATCGGATTAAGTTTATGCAAGCAAATCATG
ACTTTGCATGAAGGAAGTATTAATGTTAAGTCGGAAGTGGGTAAAGGAAGCAGTTTTATCCTTACTTTTCCTAAATAA

Upstream 100 bases:

>100_bases
AAAAAGCAATGAAACTTAGTGAAGGGAATATCTCACGTGCTGCTGACTATTTAGGTATTACCCGCTTTGCCTTATATCGC
AAACTTGAAAAACTAGGCTT

Downstream 100 bases:

>100_bases
GAGCCTTTGATATTTGCATCTTGATGTGCTATTCTTAAGATTACGTCGAAAAATTATTGTACCAAAAGCAACAAACCAGA
AAATAAACTGCATAAATAAA

Product: two-component system sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MKRFAFRVILHILLIVLFSIGSYLLFQKQLWFSTTICLILLITIGIHLYRMQFKQIALLRRLTDGLRYNDMMQTFHPPFN
NKIMNEWAEELSDTLKDFRGRLLAEEIKHQYYENLLNKVDTAVLVADKAGHIEWMNQAAVTHLGQISQLPETLLKASVAH
DTPVIRIEQNSTVLEMAISRTTFATQGREQQLISLKNIHSVLERNEMEAWQKLIRVLTHEIMNSITPIISLSETLSERGI
PSQLGEKEYSVMLQAMQTIHRRSKGLLEFVENYRRLTRIPAPIRTQISIAELFTDLKKLFPEEEFQFEVPSPELKLNVDR
TQIEQILINLLKNAREACSRKSDKKIQVKARKLSAGNTTLTISDNGEGILPDVLDKIFVPFFTTKTSGSGIGLSLCKQIM
TLHEGSINVKSEVGKGSSFILTFPK

Sequences:

>Translated_425_residues
MKRFAFRVILHILLIVLFSIGSYLLFQKQLWFSTTICLILLITIGIHLYRMQFKQIALLRRLTDGLRYNDMMQTFHPPFN
NKIMNEWAEELSDTLKDFRGRLLAEEIKHQYYENLLNKVDTAVLVADKAGHIEWMNQAAVTHLGQISQLPETLLKASVAH
DTPVIRIEQNSTVLEMAISRTTFATQGREQQLISLKNIHSVLERNEMEAWQKLIRVLTHEIMNSITPIISLSETLSERGI
PSQLGEKEYSVMLQAMQTIHRRSKGLLEFVENYRRLTRIPAPIRTQISIAELFTDLKKLFPEEEFQFEVPSPELKLNVDR
TQIEQILINLLKNAREACSRKSDKKIQVKARKLSAGNTTLTISDNGEGILPDVLDKIFVPFFTTKTSGSGIGLSLCKQIM
TLHEGSINVKSEVGKGSSFILTFPK
>Mature_425_residues
MKRFAFRVILHILLIVLFSIGSYLLFQKQLWFSTTICLILLITIGIHLYRMQFKQIALLRRLTDGLRYNDMMQTFHPPFN
NKIMNEWAEELSDTLKDFRGRLLAEEIKHQYYENLLNKVDTAVLVADKAGHIEWMNQAAVTHLGQISQLPETLLKASVAH
DTPVIRIEQNSTVLEMAISRTTFATQGREQQLISLKNIHSVLERNEMEAWQKLIRVLTHEIMNSITPIISLSETLSERGI
PSQLGEKEYSVMLQAMQTIHRRSKGLLEFVENYRRLTRIPAPIRTQISIAELFTDLKKLFPEEEFQFEVPSPELKLNVDR
TQIEQILINLLKNAREACSRKSDKKIQVKARKLSAGNTTLTISDNGEGILPDVLDKIFVPFFTTKTSGSGIGLSLCKQIM
TLHEGSINVKSEVGKGSSFILTFPK

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: COG5000

COG function: function code T; Signal transduction histidine kinase involved in nitrogen fixation and metabolism regulation

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=230, Percent_Identity=30.8695652173913, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788549, Length=220, Percent_Identity=33.1818181818182, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1786600, Length=220, Percent_Identity=24.5454545454545, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=111, Percent_Identity=36.036036036036, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 48636; Mature: 48636

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFAFRVILHILLIVLFSIGSYLLFQKQLWFSTTICLILLITIGIHLYRMQFKQIALLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLTDGLRYNDMMQTFHPPFNNKIMNEWAEELSDTLKDFRGRLLAEEIKHQYYENLLNKVD
HHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAVLVADKAGHIEWMNQAAVTHLGQISQLPETLLKASVAHDTPVIRIEQNSTVLEMAISR
HHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
TTFATQGREQQLISLKNIHSVLERNEMEAWQKLIRVLTHEIMNSITPIISLSETLSERGI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSQLGEKEYSVMLQAMQTIHRRSKGLLEFVENYRRLTRIPAPIRTQISIAELFTDLKKLF
CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
PEEEFQFEVPSPELKLNVDRTQIEQILINLLKNAREACSRKSDKKIQVKARKLSAGNTTL
CCCCCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEE
TISDNGEGILPDVLDKIFVPFFTTKTSGSGIGLSLCKQIMTLHEGSINVKSEVGKGSSFI
EECCCCCCCCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCEE
LTFPK
EECCC
>Mature Secondary Structure
MKRFAFRVILHILLIVLFSIGSYLLFQKQLWFSTTICLILLITIGIHLYRMQFKQIALLR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RLTDGLRYNDMMQTFHPPFNNKIMNEWAEELSDTLKDFRGRLLAEEIKHQYYENLLNKVD
HHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAVLVADKAGHIEWMNQAAVTHLGQISQLPETLLKASVAHDTPVIRIEQNSTVLEMAISR
HHEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHH
TTFATQGREQQLISLKNIHSVLERNEMEAWQKLIRVLTHEIMNSITPIISLSETLSERGI
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSQLGEKEYSVMLQAMQTIHRRSKGLLEFVENYRRLTRIPAPIRTQISIAELFTDLKKLF
CHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
PEEEFQFEVPSPELKLNVDRTQIEQILINLLKNAREACSRKSDKKIQVKARKLSAGNTTL
CCCCCEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCEEE
TISDNGEGILPDVLDKIFVPFFTTKTSGSGIGLSLCKQIMTLHEGSINVKSEVGKGSSFI
EECCCCCCCCHHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCHHHCCCCCCEE
LTFPK
EECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]