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The map label for this gene is pyrI [H]

Identifier: 29346151

GI number: 29346151

Start: 918977

End: 919438

Strand: Reverse

Name: pyrI [H]

Synonym: BT_0741

Alternate gene names: 29346151

Gene position: 919438-918977 (Counterclockwise)

Preceding gene: 29346152

Following gene: 29346150

Centisome position: 14.69

GC content: 43.29

Gene sequence:

>462_bases
ATGAGCGAAAATAAACAAGCATTGCAAGTAGCTGCCCTGAAAAACGGGACAGTGATTGACCATATCCCATCAGAAAAACT
CTTTACTGTCGTACAGCTACTGGGCGTGGAGCAAATGAAGTGTAACATCACCATCGGCTTCAACCTCGACAGCAAAAAGC
TAGGGAAAAAAGGCATTATCAAAATCGCCGACAAGTTTTTCTGCGACGAAGAAATCAACCGCATATCGGTAGTAGCACCT
TACGTAAAACTGAACATTATCCGCGACTATGAAGTCGTTGAAAAGAAAGAAGTCAGAATGCCGGACGAACTGCACGGCAT
CGTAAAATGCGCCAATCCGAAATGTATCACGAACAATGAACCTATGCCTACGTTATTTCATGTGATAGACAAGGATAATT
GCATCGTGAAGTGCCATTATTGCGAGAAAGAACAGAAACGCGAGGAAATCACCATCCTCTAG

Upstream 100 bases:

>100_bases
ATTATTTCCAACAGGCGCAAAACGGTCTGTATGCCCGTGAAGCCATCCTTTGCGACGTATTAGGTATCACATTAGAGGAC
GTTAAAAACGATATTTTATT

Downstream 100 bases:

>100_bases
TTTCTCCATTCCTCATTCTTAATTTTTTATTAAATTGAAACAAGACTGGAAACCGGGAACGATGATCTATCCGCTGCCTG
CCATATTGGTGAGTTGCGGA

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate

Alternate protein names: NA

Number of amino acids: Translated: 153; Mature: 152

Protein sequence:

>153_residues
MSENKQALQVAALKNGTVIDHIPSEKLFTVVQLLGVEQMKCNITIGFNLDSKKLGKKGIIKIADKFFCDEEINRISVVAP
YVKLNIIRDYEVVEKKEVRMPDELHGIVKCANPKCITNNEPMPTLFHVIDKDNCIVKCHYCEKEQKREEITIL

Sequences:

>Translated_153_residues
MSENKQALQVAALKNGTVIDHIPSEKLFTVVQLLGVEQMKCNITIGFNLDSKKLGKKGIIKIADKFFCDEEINRISVVAP
YVKLNIIRDYEVVEKKEVRMPDELHGIVKCANPKCITNNEPMPTLFHVIDKDNCIVKCHYCEKEQKREEITIL
>Mature_152_residues
SENKQALQVAALKNGTVIDHIPSEKLFTVVQLLGVEQMKCNITIGFNLDSKKLGKKGIIKIADKFFCDEEINRISVVAPY
VKLNIIRDYEVVEKKEVRMPDELHGIVKCANPKCITNNEPMPTLFHVIDKDNCIVKCHYCEKEQKREEITIL

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase [H]

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family [H]

Homologues:

Organism=Escherichia coli, GI1790692, Length=138, Percent_Identity=39.8550724637681, Blast_Score=112, Evalue=7e-27,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- ProDom:   PD006194 [H]

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C [H]

EC number: 2.1.3.2

Molecular weight: Translated: 17472; Mature: 17341

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
4.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENKQALQVAALKNGTVIDHIPSEKLFTVVQLLGVEQMKCNITIGFNLDSKKLGKKGII
CCCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECCCHHHCCCCCHH
KIADKFFCDEEINRISVVAPYVKLNIIRDYEVVEKKEVRMPDELHGIVKCANPKCITNNE
HHHHHHHCCHHCCEEEEEECEEEEEEEECCHHHHHHHCCCCHHHHHHEEECCCEEECCCC
PMPTLFHVIDKDNCIVKCHYCEKEQKREEITIL
CCCCEEEEECCCCEEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SENKQALQVAALKNGTVIDHIPSEKLFTVVQLLGVEQMKCNITIGFNLDSKKLGKKGII
CCCCCCEEEEEECCCEEEECCCCHHHHHHHHHHCCCEEEEEEEEEECCCHHHCCCCCHH
KIADKFFCDEEINRISVVAPYVKLNIIRDYEVVEKKEVRMPDELHGIVKCANPKCITNNE
HHHHHHHCCHHCCEEEEEECEEEEEEEECCHHHHHHHCCCCHHHHHHEEECCCEEECCCC
PMPTLFHVIDKDNCIVKCHYCEKEQKREEITIL
CCCCEEEEECCCCEEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA