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The map label for this gene is spsK [H]

Identifier: 29346135

GI number: 29346135

Start: 899030

End: 899938

Strand: Reverse

Name: spsK [H]

Synonym: BT_0725

Alternate gene names: 29346135

Gene position: 899938-899030 (Counterclockwise)

Preceding gene: 29346137

Following gene: 29346134

Centisome position: 14.38

GC content: 45.54

Gene sequence:

>909_bases
ATGAAGAATATAATAATTATCGGTGCCAATGGATTCACCGGACGCCAAATACTGAATGACCTGTCGAGCAAGGCACAATA
TAAGGTAACAGGATGTTCCCTGCATCCGGATATCCTTCCGAAGAATGGAGGCAACTATCATTTCATCACCACAGATATAC
GGGATGAAGCAGTCGTAAAGCAACTTTTTAAAGACGTACAACCCGATGTAGTCATCAACTGTTCCGCCTTATCCGTCCCC
GATTATTGCGAAACGCATCACGAAGAAGCCTGGCTAACAAATGTTACGGCAGTAGAACAATTGGCACATCTTTGCGAAAG
TTACAAAAGCCGGTTCATTCATTTATCTACAGACTTCGTCTTTGACGGAAAGATAGACGAGAAGTCCGGACAGCTTTATA
CAGAAAAAAGTCTCCCTGCTCCCGTCAACTATTACGGTTTCACCAAATGGAAAGGAGAAGAGAAAGTGACTGAAATCTGT
AGTAACTATGCTATTGCCCGCGTAGAGATTGTCTACGGAAAAGCATTGCCGGGACAACATGGAAACATCGTGCAACTGGT
GATGAATCGTTTGAATGCAGGACAAGAAATCCGTGTCGTATCAGATCAATGGCGCACGCCCACCTATGTAGGTGACGTAT
CAGCCGGAGTACAACACCTGGTAGAAAATGCCGCAAACGGAATTTTTCATATCTGTGGCGAGGAATGCCTGACCATCGCA
GAAATTGCTTTTCAGGTAGCAGACTACATGAAGCTGGACCGTTCGCTCGTTCACCCTGCCACAACAGAAGAAATGCAAGA
AGCTACTCCCCGTCCCCGTTTCAGTGGGATGAGTATAGCAAAAGCCCGGACTATACTTGGTTACCAGCCACGAAAGCTGA
AAGACATCCTCATGGAGTGGAAACATTAA

Upstream 100 bases:

>100_bases
TTTGTGAAAATAAATATAGAAAAGCCATTTCTGAACCGAAACTCACTCATGTGACTGAGAAAAACTCATATGACTGAAAC
GAACGACTATAAAACAGCCC

Downstream 100 bases:

>100_bases
CCGACAAATCCTATTAATCAGATTGTTTGCACAAATAAAAGTATGTGCTCTACACAACATAGAAATTTGACTCAGGCATC
GAACTGGCAACTAAATAAAA

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-L-rhamnose; Nicotinamide adenine dinucleotide phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKNIIIIGANGFTGRQILNDLSSKAQYKVTGCSLHPDILPKNGGNYHFITTDIRDEAVVKQLFKDVQPDVVINCSALSVP
DYCETHHEEAWLTNVTAVEQLAHLCESYKSRFIHLSTDFVFDGKIDEKSGQLYTEKSLPAPVNYYGFTKWKGEEKVTEIC
SNYAIARVEIVYGKALPGQHGNIVQLVMNRLNAGQEIRVVSDQWRTPTYVGDVSAGVQHLVENAANGIFHICGEECLTIA
EIAFQVADYMKLDRSLVHPATTEEMQEATPRPRFSGMSIAKARTILGYQPRKLKDILMEWKH

Sequences:

>Translated_302_residues
MKNIIIIGANGFTGRQILNDLSSKAQYKVTGCSLHPDILPKNGGNYHFITTDIRDEAVVKQLFKDVQPDVVINCSALSVP
DYCETHHEEAWLTNVTAVEQLAHLCESYKSRFIHLSTDFVFDGKIDEKSGQLYTEKSLPAPVNYYGFTKWKGEEKVTEIC
SNYAIARVEIVYGKALPGQHGNIVQLVMNRLNAGQEIRVVSDQWRTPTYVGDVSAGVQHLVENAANGIFHICGEECLTIA
EIAFQVADYMKLDRSLVHPATTEEMQEATPRPRFSGMSIAKARTILGYQPRKLKDILMEWKH
>Mature_302_residues
MKNIIIIGANGFTGRQILNDLSSKAQYKVTGCSLHPDILPKNGGNYHFITTDIRDEAVVKQLFKDVQPDVVINCSALSVP
DYCETHHEEAWLTNVTAVEQLAHLCESYKSRFIHLSTDFVFDGKIDEKSGQLYTEKSLPAPVNYYGFTKWKGEEKVTEIC
SNYAIARVEIVYGKALPGQHGNIVQLVMNRLNAGQEIRVVSDQWRTPTYVGDVSAGVQHLVENAANGIFHICGEECLTIA
EIAFQVADYMKLDRSLVHPATTEEMQEATPRPRFSGMSIAKARTILGYQPRKLKDILMEWKH

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=289, Percent_Identity=28.3737024221453, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI11034825, Length=289, Percent_Identity=28.3737024221453, Blast_Score=115, Evalue=5e-26,
Organism=Escherichia coli, GI1788352, Length=192, Percent_Identity=28.6458333333333, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=311, Percent_Identity=25.4019292604502, Blast_Score=71, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133 [C]

Molecular weight: Translated: 33904; Mature: 33904

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIIIIGANGFTGRQILNDLSSKAQYKVTGCSLHPDILPKNGGNYHFITTDIRDEAVVK
CCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHH
QLFKDVQPDVVINCSALSVPDYCETHHEEAWLTNVTAVEQLAHLCESYKSRFIHLSTDFV
HHHHHCCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECEE
FDGKIDEKSGQLYTEKSLPAPVNYYGFTKWKGEEKVTEICSNYAIARVEIVYGKALPGQH
ECCCCCCCCCCEEECCCCCCCCCCCCCEEECCHHHHHHHHCCCCEEEEEEEEECCCCCCC
GNIVQLVMNRLNAGQEIRVVSDQWRTPTYVGDVSAGVQHLVENAANGIFHICGEECLTIA
CCHHHHHHHHHCCCCEEEEECCCCCCCCEECHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
EIAFQVADYMKLDRSLVHPATTEEMQEATPRPRFSGMSIAKARTILGYQPRKLKDILMEW
HHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHC
KH
CC
>Mature Secondary Structure
MKNIIIIGANGFTGRQILNDLSSKAQYKVTGCSLHPDILPKNGGNYHFITTDIRDEAVVK
CCCEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEECCCHHHHHH
QLFKDVQPDVVINCSALSVPDYCETHHEEAWLTNVTAVEQLAHLCESYKSRFIHLSTDFV
HHHHHCCCCEEEEEECCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCEEEEEECEE
FDGKIDEKSGQLYTEKSLPAPVNYYGFTKWKGEEKVTEICSNYAIARVEIVYGKALPGQH
ECCCCCCCCCCEEECCCCCCCCCCCCCEEECCHHHHHHHHCCCCEEEEEEEEECCCCCCC
GNIVQLVMNRLNAGQEIRVVSDQWRTPTYVGDVSAGVQHLVENAANGIFHICGEECLTIA
CCHHHHHHHHHCCCCEEEEECCCCCCCCEECHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
EIAFQVADYMKLDRSLVHPATTEEMQEATPRPRFSGMSIAKARTILGYQPRKLKDILMEW
HHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHC
KH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-L-mannose; H+; Nicotinamide adenine dinucleotide phosphate - reduced [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-L-mannose + H+ + Nicotinamide adenine dinucleotide phosphate - reduced --> dTDP-L-rhamnose + Nicotinamide adenine dinucleotide phosphate [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]