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The map label for this gene is moeZ [H]

Identifier: 29346058

GI number: 29346058

Start: 798328

End: 799020

Strand: Reverse

Name: moeZ [H]

Synonym: BT_0648

Alternate gene names: 29346058

Gene position: 799020-798328 (Counterclockwise)

Preceding gene: 29346059

Following gene: 29346057

Centisome position: 12.76

GC content: 45.6

Gene sequence:

>693_bases
ATGCGGTACGACAGGCAAATGCTCCTTCCCGAAATCGGAGAAGACGGTCAGCAGAAACTGAAGCAAGCAAAGGTTCTTAT
TGTAGGCGTGGGAGGGCTAGGCTCTCCCATAGCCCTCTATCTGACCGGAGCAGGAGTAGGCTGCATTGGATTGGTAGACG
ACGATGTAGTCAGTATCAGTAATCTGCAACGGCAAGTGCTCTATTCGGAGAAAGAATTAGGTAAACCCAAGGCAATATGT
GCCGCCGAACGACTTTCCGCACTTAACAGCGAGATAACTATCCGGACTTATCCAACCCGTTTGACAGAGGAAAACGCACA
GGAAATTATCAGTCAGTATGATATAGTAGTAGATGGCTGCGACAATTTCTCTACCCGTTATCTTATCAATGACATCTGTG
CGGAAATGGGGAAAGTGTATGTCTATGGAGCAATCTGCGGCTTTGAAGGTCAGGTATCCGTTTTCCATTACGGAGAAGAG
AAAAAAAGTTATCGGGACTTATATCCGGATGAAGAAGAAATGAGACGAATGCCCCCGCCTCCCAAAGGTGTAATGGGCAT
TACTCCTGCGGTAACAGGAAGCATCGAAGCAACGGAAGTGCTGAAAATTATCTGTGGTTTTGGCGAAGTATTATCGGGTA
AACTATGGACTATTGACTTGCGTACATTGCAATCTAACAAATTTTCACTATAA

Upstream 100 bases:

>100_bases
GAACGGAAAGCGGTAGAAGTGGAACGCGACCTGAGGAAATTAGGACGTGAACCGGTCTGGAAGGATTGGGACCAGTCTTT
TGACTTTAAAAGATAAAACG

Downstream 100 bases:

>100_bases
ACGTTGGTTTCTGAAATAGTTAATTAGTAACTTTGCTAAACTTTACAACTTTACAAAGGAAATGAAACTAATTGTAGTTA
CCACGCCCACGTTCTTCGTT

Product: molybdopterin biosynthesis protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRYDRQMLLPEIGEDGQQKLKQAKVLIVGVGGLGSPIALYLTGAGVGCIGLVDDDVVSISNLQRQVLYSEKELGKPKAIC
AAERLSALNSEITIRTYPTRLTEENAQEIISQYDIVVDGCDNFSTRYLINDICAEMGKVYVYGAICGFEGQVSVFHYGEE
KKSYRDLYPDEEEMRRMPPPPKGVMGITPAVTGSIEATEVLKIICGFGEVLSGKLWTIDLRTLQSNKFSL

Sequences:

>Translated_230_residues
MRYDRQMLLPEIGEDGQQKLKQAKVLIVGVGGLGSPIALYLTGAGVGCIGLVDDDVVSISNLQRQVLYSEKELGKPKAIC
AAERLSALNSEITIRTYPTRLTEENAQEIISQYDIVVDGCDNFSTRYLINDICAEMGKVYVYGAICGFEGQVSVFHYGEE
KKSYRDLYPDEEEMRRMPPPPKGVMGITPAVTGSIEATEVLKIICGFGEVLSGKLWTIDLRTLQSNKFSL
>Mature_230_residues
MRYDRQMLLPEIGEDGQQKLKQAKVLIVGVGGLGSPIALYLTGAGVGCIGLVDDDVVSISNLQRQVLYSEKELGKPKAIC
AAERLSALNSEITIRTYPTRLTEENAQEIISQYDIVVDGCDNFSTRYLINDICAEMGKVYVYGAICGFEGQVSVFHYGEE
KKSYRDLYPDEEEMRRMPPPPKGVMGITPAVTGSIEATEVLKIICGFGEVLSGKLWTIDLRTLQSNKFSL

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=227, Percent_Identity=44.0528634361233, Blast_Score=171, Evalue=5e-43,
Organism=Homo sapiens, GI150417996, Length=186, Percent_Identity=30.6451612903226, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI4885649, Length=136, Percent_Identity=29.4117647058824, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI38045948, Length=163, Percent_Identity=32.5153374233129, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI38045944, Length=117, Percent_Identity=34.1880341880342, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI38045942, Length=117, Percent_Identity=34.1880341880342, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87082356, Length=230, Percent_Identity=39.5652173913043, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1787048, Length=230, Percent_Identity=37.8260869565217, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1789177, Length=122, Percent_Identity=31.1475409836066, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=237, Percent_Identity=41.3502109704641, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI193203301, Length=144, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71984476, Length=174, Percent_Identity=28.1609195402299, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=214, Percent_Identity=41.588785046729, Blast_Score=155, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6320598, Length=148, Percent_Identity=29.7297297297297, Blast_Score=80, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6321965, Length=178, Percent_Identity=29.2134831460674, Blast_Score=66, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6322639, Length=179, Percent_Identity=31.2849162011173, Blast_Score=65, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24582879, Length=226, Percent_Identity=42.0353982300885, Blast_Score=172, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24660640, Length=143, Percent_Identity=30.0699300699301, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI28573937, Length=140, Percent_Identity=30, Blast_Score=72, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 25355; Mature: 25355

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYDRQMLLPEIGEDGQQKLKQAKVLIVGVGGLGSPIALYLTGAGVGCIGLVDDDVVSIS
CCCCCCCCCCCCCCHHHHHHHHCEEEEEECCCCCCCEEEEEECCCCEEEEECCCCHHHHH
NLQRQVLYSEKELGKPKAICAAERLSALNSEITIRTYPTRLTEENAQEIISQYDIVVDGC
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCHHCHHHHHHHHHHCCEEEECC
DNFSTRYLINDICAEMGKVYVYGAICGFEGQVSVFHYGEEKKSYRDLYPDEEEMRRMPPP
CCCCHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEEECCHHHHHHCCCCCHHHHHCCCCC
PKGVMGITPAVTGSIEATEVLKIICGFGEVLSGKLWTIDLRTLQSNKFSL
CCCCEECCCCCCCCCHHHHHHHHHHCCHHHHCCCEEEEEEEEECCCCCCC
>Mature Secondary Structure
MRYDRQMLLPEIGEDGQQKLKQAKVLIVGVGGLGSPIALYLTGAGVGCIGLVDDDVVSIS
CCCCCCCCCCCCCCHHHHHHHHCEEEEEECCCCCCCEEEEEECCCCEEEEECCCCHHHHH
NLQRQVLYSEKELGKPKAICAAERLSALNSEITIRTYPTRLTEENAQEIISQYDIVVDGC
HHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCHHCHHHHHHHHHHCCEEEECC
DNFSTRYLINDICAEMGKVYVYGAICGFEGQVSVFHYGEEKKSYRDLYPDEEEMRRMPPP
CCCCHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEEECCHHHHHHCCCCCHHHHHCCCCC
PKGVMGITPAVTGSIEATEVLKIICGFGEVLSGKLWTIDLRTLQSNKFSL
CCCCEECCCCCCCCCHHHHHHHHHHCCHHHHCCCEEEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]