Click here to switch to the map view.

The map label for this gene is trpB

Identifier: 29345943

GI number: 29345943

Start: 656402

End: 657586

Strand: Reverse

Name: trpB

Synonym: BT_0533

Alternate gene names: 29345943

Gene position: 657586-656402 (Counterclockwise)

Preceding gene: 29345944

Following gene: 29345942

Centisome position: 10.5

GC content: 47.76

Gene sequence:

>1185_bases
ATGAAAAGTTTTTTAGTTGATCAGGATGGTTACTACGGAGAGTTCGGCGGCGCTTATGTTCCGGAAATTCTCCACAAATG
TGTAGAGGAACTGAAGAATACGTACCTCGGAGTGCTCGAAAGCGAAGATTTCAAAAAGGAGTTTGACCAGTTGCTGCGTG
ACTACGTAGGCCGCCCTTCCCCACTCTATCTCGCCCGCAGACTTTCAGAAAAGTATGGTTGCAAAATGTATCTGAAACGG
GAAGACCTGAACCATACAGGTGCCCACAAAATCAATAATACTATCGGACAGATTTTGCTGGCACGCCGCATGGGAAAGAA
ACGTATTATCGCTGAAACCGGAGCCGGACAACACGGCGTTGCAACGGCTACCGTATGTGCTTTGATGGATATGGAATGTA
TTGTCTATATGGGTAAAACGGACGTTGAGCGCCAGCACATCAATGTCGAAAAAATGAAGATGCTGGGAGCTACCGTAATC
CCAGTCACTTCCGGTAACATGACACTGAAAGATGCGACCAACGAAGCAATCCGCGACTGGTGCTGTCATCCTGCCGACAC
TTATTATATCATCGGCTCAACAGTAGGTCCCCATCCTTATCCCGACATGGTGGCACGCCTGCAATCCGTAATCAGCGAAG
AAATCAAAAAACAACTTATGGAGAAAGAAGGACGTGATCACCCTGATTATCTGATAGCCTGTGTAGGTGGAGGAAGCAAT
GCCGCCGGAACGATTTATCATTATATCAATGACGGACGGGTAGGCATCATTCTGGCAGAAGCAGGAGGAAAAGGAATAGA
AACCGGAATGACCGCCGCCACTATACAGCTTGGGAAAATGGGCATTATCCATGGAGCCCGTACTTACGTTATCCAGAACG
AAGACGGACAGATTGAAGAACCCTACTCTATCTCCGCCGGACTGGACTATCCGGGAATCGGACCGATACACGCCAATCTT
GCCGCTCAAAGACGGGCTACCGTATTGGCTGTCAATGATGACGAAGCAATAGAAGCCGCTTATGAACTGACCAAACTTGA
AGGAATCATTCCCGCACTGGAATCTGCCCACGCACTGGGTGCGCTGAAAAAATTGAAGTTCAAACCGGAAGATGTGGTTG
TACTGACCGTATCGGGACGTGGCGACAAAGACATTGAAACTTATTTGTCTTTCAATGAAAAGTGA

Upstream 100 bases:

>100_bases
ATAACAAATTTGAAAGAGCCCTGTCGCAACCTGCGACAGGGCTCTTTTTATTTGCCCGTTTTTGAAAATCGAATAAATAA
TAATAAATAAAAATAGGATT

Downstream 100 bases:

>100_bases
TTATCAACATCTAGTTTTGTAGTTTGAATTTAGAAATTATAAAAATGAAAACATTCAGTTATACAGCCCATAGCAAACAG
GTACTCGGAGATATGCACAC

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MKSFLVDQDGYYGEFGGAYVPEILHKCVEELKNTYLGVLESEDFKKEFDQLLRDYVGRPSPLYLARRLSEKYGCKMYLKR
EDLNHTGAHKINNTIGQILLARRMGKKRIIAETGAGQHGVATATVCALMDMECIVYMGKTDVERQHINVEKMKMLGATVI
PVTSGNMTLKDATNEAIRDWCCHPADTYYIIGSTVGPHPYPDMVARLQSVISEEIKKQLMEKEGRDHPDYLIACVGGGSN
AAGTIYHYINDGRVGIILAEAGGKGIETGMTAATIQLGKMGIIHGARTYVIQNEDGQIEEPYSISAGLDYPGIGPIHANL
AAQRRATVLAVNDDEAIEAAYELTKLEGIIPALESAHALGALKKLKFKPEDVVVLTVSGRGDKDIETYLSFNEK

Sequences:

>Translated_394_residues
MKSFLVDQDGYYGEFGGAYVPEILHKCVEELKNTYLGVLESEDFKKEFDQLLRDYVGRPSPLYLARRLSEKYGCKMYLKR
EDLNHTGAHKINNTIGQILLARRMGKKRIIAETGAGQHGVATATVCALMDMECIVYMGKTDVERQHINVEKMKMLGATVI
PVTSGNMTLKDATNEAIRDWCCHPADTYYIIGSTVGPHPYPDMVARLQSVISEEIKKQLMEKEGRDHPDYLIACVGGGSN
AAGTIYHYINDGRVGIILAEAGGKGIETGMTAATIQLGKMGIIHGARTYVIQNEDGQIEEPYSISAGLDYPGIGPIHANL
AAQRRATVLAVNDDEAIEAAYELTKLEGIIPALESAHALGALKKLKFKPEDVVVLTVSGRGDKDIETYLSFNEK
>Mature_394_residues
MKSFLVDQDGYYGEFGGAYVPEILHKCVEELKNTYLGVLESEDFKKEFDQLLRDYVGRPSPLYLARRLSEKYGCKMYLKR
EDLNHTGAHKINNTIGQILLARRMGKKRIIAETGAGQHGVATATVCALMDMECIVYMGKTDVERQHINVEKMKMLGATVI
PVTSGNMTLKDATNEAIRDWCCHPADTYYIIGSTVGPHPYPDMVARLQSVISEEIKKQLMEKEGRDHPDYLIACVGGGSN
AAGTIYHYINDGRVGIILAEAGGKGIETGMTAATIQLGKMGIIHGARTYVIQNEDGQIEEPYSISAGLDYPGIGPIHANL
AAQRRATVLAVNDDEAIEAAYELTKLEGIIPALESAHALGALKKLKFKPEDVVVLTVSGRGDKDIETYLSFNEK

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG0133

COG function: function code E; Tryptophan synthase beta chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=380, Percent_Identity=52.1052631578947, Blast_Score=396, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6321412, Length=379, Percent_Identity=49.868073878628, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_BACTN (Q8AAD2)

Other databases:

- EMBL:   AE015928
- RefSeq:   NP_809446.1
- ProteinModelPortal:   Q8AAD2
- SMR:   Q8AAD2
- GeneID:   1071998
- GenomeReviews:   AE015928_GR
- KEGG:   bth:BT_0533
- NMPDR:   fig|226186.1.peg.533
- HOGENOM:   HBG303148
- OMA:   LKREDLC
- ProtClustDB:   PRK04346
- BioCyc:   BTHE226186:BT_0533-MONOMER
- BRENDA:   4.2.1.20
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006653
- InterPro:   IPR006654
- InterPro:   IPR023026
- TIGRFAMs:   TIGR00263

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 43275; Mature: 43275

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSFLVDQDGYYGEFGGAYVPEILHKCVEELKNTYLGVLESEDFKKEFDQLLRDYVGRPS
CCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCC
PLYLARRLSEKYGCKMYLKREDLNHTGAHKINNTIGQILLARRMGKKRIIAETGAGQHGV
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
ATATVCALMDMECIVYMGKTDVERQHINVEKMKMLGATVIPVTSGNMTLKDATNEAIRDW
HHHHHHHHHCCEEEEEECCCCCCHHHCCHHHHHHHCCEEEEEECCCEEEECCCHHHHHHH
CCHPADTYYIIGSTVGPHPYPDMVARLQSVISEEIKKQLMEKEGRDHPDYLIACVGGGSN
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
AAGTIYHYINDGRVGIILAEAGGKGIETGMTAATIQLGKMGIIHGARTYVIQNEDGQIEE
CCEEEEEEECCCEEEEEEECCCCCCCCCCCEEEEEEECCEEEEECCEEEEEECCCCCCCC
PYSISAGLDYPGIGPIHANLAAQRRATVLAVNDDEAIEAAYELTKLEGIIPALESAHALG
CCEEECCCCCCCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKKLKFKPEDVVVLTVSGRGDKDIETYLSFNEK
HHHHCCCCCCCEEEEEECCCCCCCHHHHHCCCCC
>Mature Secondary Structure
MKSFLVDQDGYYGEFGGAYVPEILHKCVEELKNTYLGVLESEDFKKEFDQLLRDYVGRPS
CCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCC
PLYLARRLSEKYGCKMYLKREDLNHTGAHKINNTIGQILLARRMGKKRIIAETGAGQHGV
HHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCH
ATATVCALMDMECIVYMGKTDVERQHINVEKMKMLGATVIPVTSGNMTLKDATNEAIRDW
HHHHHHHHHCCEEEEEECCCCCCHHHCCHHHHHHHCCEEEEEECCCEEEECCCHHHHHHH
CCHPADTYYIIGSTVGPHPYPDMVARLQSVISEEIKKQLMEKEGRDHPDYLIACVGGGSN
CCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
AAGTIYHYINDGRVGIILAEAGGKGIETGMTAATIQLGKMGIIHGARTYVIQNEDGQIEE
CCEEEEEEECCCEEEEEEECCCCCCCCCCCEEEEEEECCEEEEECCEEEEEECCCCCCCC
PYSISAGLDYPGIGPIHANLAAQRRATVLAVNDDEAIEAAYELTKLEGIIPALESAHALG
CCEEECCCCCCCCCCHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ALKKLKFKPEDVVVLTVSGRGDKDIETYLSFNEK
HHHHCCCCCCCEEEEEECCCCCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12663928