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The map label for this gene is trpE [H]

Identifier: 29345942

GI number: 29345942

Start: 654951

End: 656357

Strand: Reverse

Name: trpE [H]

Synonym: BT_0532

Alternate gene names: 29345942

Gene position: 656357-654951 (Counterclockwise)

Preceding gene: 29345943

Following gene: 29345941

Centisome position: 10.48

GC content: 45.42

Gene sequence:

>1407_bases
ATGAAAACATTCAGTTATACAGCCCATAGCAAACAGGTACTCGGAGATATGCACACTCCTGTCAGTATCTATCTGAAAGT
ACGTGATATGTACCCACAATCTGCATTGATGGAGAGTTCCGATTATCATGCAGGAGAAAATTCCCTCTCGTTCATCGCTC
TTTGTCCGCTGGCAAGTATCGGTGTCAATAGCGGAATAGTCACTGCTAGTTATCCTGACAAAAGTCGGAAGGAAGAGCCG
CTCACCCAATCATTCACGGTAGAAAAAGCGATGAATCAATTCATCAGTCAGTTTCAAGTGACCGGAGAAAACAAGAATGT
GTGCGGACTTTACGGATACACCACATTCAACGCAGTGAAATACTTCGAACATATCCCGGTGAAAGAAAGTCATGATGAGC
AGAATGATGCTCCGGACTTGCTATACATATTATATAAGTATATCATCGTTTTCAATCATTTCAAGAATGAATTGACATTG
GTAGAAATGCTGGGCGAAGGTGAAGAAAGCGGTTTGCCGGAACTGGAGGCCGCTATCGAAAACAGAAATTATGCTTCTTA
TAATTTCTCGGTGACAGGTCCTGTCAGCAGCCCGATCTCCGATGAGGAGCATAAAGCGAACGTTCGCAAGGGTATTGCCC
ATTGTATGCGTGGTGATGTTTTTCAGATTGTCCTTTCCAGAAGATTCATCCAGCCTTATGCCGGAGATGATTTCAAAGTC
TATCGTGCTCTTCGCAGCATCAACCCTTCTCCTTATCTTTTCTATTTCGATTTCGGAGGATACCGCATTTTCGGTTCTTC
TCCGGAGACTCACTGTAAAATTGAAGACGGACGTGCCTACATCGACCCGATTGCCGGAACAACCCGCCGTACCGGAGATA
CGGTAAAAGACCGCGAATTGGCGGAAGCACTGCTTGCCGATCCGAAAGAGAACGCGGAGCATGTGATGCTGGTTGATCTG
GCAAGAAATGATCTTTCACGTAATTGCCATGATGTACGGGTATTGTTTTATAAGGAACCACAGTATTACAGTCATGTGAT
TCATCTGGTCAGCCGCGTGAGCGGTCAGCTGAATGAAGGGGCGGATAAGATTAAAACTTTCATCGATACTTTCCCTGCGG
GAACGTTGAGCGGAGCACCGAAAGTACGCGCCATGCAGCTGATCAGTGAAATCGAACCTCATAACCGCGGTGCTTATGGC
GGCTGTATCGGCTTTATCGGACTTAACGGAGAACTGAACCAGGCAATCACCATCCGTACTTTTGTCAGCCGTAATAACGA
GTTATGGTTCCAGGCCGGAGGCGGTATCGTTGCACGCAGTCAGGATGAATACGAGTTGCAAGAGGTAAACAATAAATTAG
GAGCTCTTAAAAAGGCTATCGATTTAGCTGTTAATTTAAAAAACTAA

Upstream 100 bases:

>100_bases
TATCGGGACGTGGCGACAAAGACATTGAAACTTATTTGTCTTTCAATGAAAAGTGATTATCAACATCTAGTTTTGTAGTT
TGAATTTAGAAATTATAAAA

Downstream 100 bases:

>100_bases
CTGTTCAGAGCGATCTGCCTGATAAGTCAGAAACAGTATTTGAACAACAATTTTACAATAACAATAAAATGAAAATATTA
CTTTTAGATAACTATGATTC

Product: anthranilate synthase component I

Products: NA

Alternate protein names: Anthranilate synthase component I [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKTFSYTAHSKQVLGDMHTPVSIYLKVRDMYPQSALMESSDYHAGENSLSFIALCPLASIGVNSGIVTASYPDKSRKEEP
LTQSFTVEKAMNQFISQFQVTGENKNVCGLYGYTTFNAVKYFEHIPVKESHDEQNDAPDLLYILYKYIIVFNHFKNELTL
VEMLGEGEESGLPELEAAIENRNYASYNFSVTGPVSSPISDEEHKANVRKGIAHCMRGDVFQIVLSRRFIQPYAGDDFKV
YRALRSINPSPYLFYFDFGGYRIFGSSPETHCKIEDGRAYIDPIAGTTRRTGDTVKDRELAEALLADPKENAEHVMLVDL
ARNDLSRNCHDVRVLFYKEPQYYSHVIHLVSRVSGQLNEGADKIKTFIDTFPAGTLSGAPKVRAMQLISEIEPHNRGAYG
GCIGFIGLNGELNQAITIRTFVSRNNELWFQAGGGIVARSQDEYELQEVNNKLGALKKAIDLAVNLKN

Sequences:

>Translated_468_residues
MKTFSYTAHSKQVLGDMHTPVSIYLKVRDMYPQSALMESSDYHAGENSLSFIALCPLASIGVNSGIVTASYPDKSRKEEP
LTQSFTVEKAMNQFISQFQVTGENKNVCGLYGYTTFNAVKYFEHIPVKESHDEQNDAPDLLYILYKYIIVFNHFKNELTL
VEMLGEGEESGLPELEAAIENRNYASYNFSVTGPVSSPISDEEHKANVRKGIAHCMRGDVFQIVLSRRFIQPYAGDDFKV
YRALRSINPSPYLFYFDFGGYRIFGSSPETHCKIEDGRAYIDPIAGTTRRTGDTVKDRELAEALLADPKENAEHVMLVDL
ARNDLSRNCHDVRVLFYKEPQYYSHVIHLVSRVSGQLNEGADKIKTFIDTFPAGTLSGAPKVRAMQLISEIEPHNRGAYG
GCIGFIGLNGELNQAITIRTFVSRNNELWFQAGGGIVARSQDEYELQEVNNKLGALKKAIDLAVNLKN
>Mature_468_residues
MKTFSYTAHSKQVLGDMHTPVSIYLKVRDMYPQSALMESSDYHAGENSLSFIALCPLASIGVNSGIVTASYPDKSRKEEP
LTQSFTVEKAMNQFISQFQVTGENKNVCGLYGYTTFNAVKYFEHIPVKESHDEQNDAPDLLYILYKYIIVFNHFKNELTL
VEMLGEGEESGLPELEAAIENRNYASYNFSVTGPVSSPISDEEHKANVRKGIAHCMRGDVFQIVLSRRFIQPYAGDDFKV
YRALRSINPSPYLFYFDFGGYRIFGSSPETHCKIEDGRAYIDPIAGTTRRTGDTVKDRELAEALLADPKENAEHVMLVDL
ARNDLSRNCHDVRVLFYKEPQYYSHVIHLVSRVSGQLNEGADKIKTFIDTFPAGTLSGAPKVRAMQLISEIEPHNRGAYG
GCIGFIGLNGELNQAITIRTFVSRNNELWFQAGGGIVARSQDEYELQEVNNKLGALKKAIDLAVNLKN

Specific function: Tryptophan biosynthesis; first step. [C]

COG id: COG0147

COG function: function code EH; Anthranilate/para-aminobenzoate synthases component I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anthranilate synthase component I family [H]

Homologues:

Organism=Escherichia coli, GI1787518, Length=398, Percent_Identity=34.9246231155779, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788114, Length=358, Percent_Identity=31.0055865921788, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1786809, Length=252, Percent_Identity=28.968253968254, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI87082077, Length=221, Percent_Identity=29.8642533936652, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320935, Length=462, Percent_Identity=34.8484848484849, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6324361, Length=376, Percent_Identity=26.3297872340426, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005801
- InterPro:   IPR019999
- InterPro:   IPR006805
- InterPro:   IPR005256
- InterPro:   IPR015890 [H]

Pfam domain/function: PF04715 Anth_synt_I_N; PF00425 Chorismate_bind [H]

EC number: =4.1.3.27 [H]

Molecular weight: Translated: 52456; Mature: 52456

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTFSYTAHSKQVLGDMHTPVSIYLKVRDMYPQSALMESSDYHAGENSLSFIALCPLASI
CCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCCCEEEEECCHHHC
GVNSGIVTASYPDKSRKEEPLTQSFTVEKAMNQFISQFQVTGENKNVCGLYGYTTFNAVK
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCHHHHHHH
YFEHIPVKESHDEQNDAPDLLYILYKYIIVFNHFKNELTLVEMLGEGEESGLPELEAAIE
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHCCCCCCCCCHHHHHHC
NRNYASYNFSVTGPVSSPISDEEHKANVRKGIAHCMRGDVFQIVLSRRFIQPYAGDDFKV
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHH
YRALRSINPSPYLFYFDFGGYRIFGSSPETHCKIEDGRAYIDPIAGTTRRTGDTVKDREL
HHHHHHCCCCCEEEEEECCCEEEECCCCCCEEEEECCCEEECCCCCCCCCCCCCCHHHHH
AEALLADPKENAEHVMLVDLARNDLSRNCHDVRVLFYKEPQYYSHVIHLVSRVSGQLNEG
HHHHHCCCCCCCCEEEEEEEHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCC
ADKIKTFIDTFPAGTLSGAPKVRAMQLISEIEPHNRGAYGGCIGFIGLNGELNQAITIRT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEE
FVSRNNELWFQAGGGIVARSQDEYELQEVNNKLGALKKAIDLAVNLKN
EECCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEECCC
>Mature Secondary Structure
MKTFSYTAHSKQVLGDMHTPVSIYLKVRDMYPQSALMESSDYHAGENSLSFIALCPLASI
CCCCCCCCHHHHHHHCCCCCEEEEEEEECCCCHHHHHCCCCCCCCCCCCEEEEECCHHHC
GVNSGIVTASYPDKSRKEEPLTQSFTVEKAMNQFISQFQVTGENKNVCGLYGYTTFNAVK
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCHHHHHHH
YFEHIPVKESHDEQNDAPDLLYILYKYIIVFNHFKNELTLVEMLGEGEESGLPELEAAIE
HHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEHHHHHCCCCCCCCCHHHHHHC
NRNYASYNFSVTGPVSSPISDEEHKANVRKGIAHCMRGDVFQIVLSRRFIQPYAGDDFKV
CCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHH
YRALRSINPSPYLFYFDFGGYRIFGSSPETHCKIEDGRAYIDPIAGTTRRTGDTVKDREL
HHHHHHCCCCCEEEEEECCCEEEECCCCCCEEEEECCCEEECCCCCCCCCCCCCCHHHHH
AEALLADPKENAEHVMLVDLARNDLSRNCHDVRVLFYKEPQYYSHVIHLVSRVSGQLNEG
HHHHHCCCCCCCCEEEEEEEHHHHHCCCCCEEEEEEEECCHHHHHHHHHHHHHCCCCCCC
ADKIKTFIDTFPAGTLSGAPKVRAMQLISEIEPHNRGAYGGCIGFIGLNGELNQAITIRT
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEEE
FVSRNNELWFQAGGGIVARSQDEYELQEVNNKLGALKKAIDLAVNLKN
EECCCCEEEEEECCCEEECCCCHHHHHHHHHHHHHHHHHHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7685830; 10360571 [H]