Click here to switch to the map view.

The map label for this gene is hsdS [H]

Identifier: 29144809

GI number: 29144809

Start: 4724923

End: 4726311

Strand: Reverse

Name: hsdS [H]

Synonym: t4574

Alternate gene names: 29144809

Gene position: 4726311-4724923 (Counterclockwise)

Preceding gene: 29144810

Following gene: 29144808

Centisome position: 98.63

GC content: 42.55

Gene sequence:

>1389_bases
ATGAGTGGGGGGAAATTGCCGGAGGGGTGGGTGACAACCCATCTTTCAGAAATATGCAGTAAGCCTCAATATGGCTACAC
AACTAAGTCAAGCTCTATGGGGGATGTAAAATTTTTAAGAACAACCGATATTACAAAAGGAGCGGTTGACTGGTCCAGTG
TACCTTATTGCATGGATGCCCCGGAAGATGTTTCAAAATACCAATTACAGGACAGAGATATTGTGATATCTCGAGCCGGT
TCGGTTGGGTTTAGTTTTTTGGTACAAAACCCTCCAAGCCAAGTTGTTTTTGCTTCATACCTTATTCGATTTAAACCAGT
TAATTATTTTTCAGAATATTACTTAAAACGTTTTTTAGAGTCGAGTGACTATTGGAATCAGCTGTCTTTAATGTCAGCAG
GGAATGCTGTACAAAATGTTAATGCTCAAAAGTTATCAACACTTACAGTACCAATACCACCTATAGCCGAACAAAAAATC
ATCGCCGAAAAACTCGATACGCTGCTGGCACAGGTAGACAGCACCAAAGCACGTCTTGAGCAAATCCCACAAATCCTGAA
ACGTTTTCGTCAGGCGGTGCTAGCTGCTGCGGTAAGCGGGTTGTTAATTGGTTCAAATAAAAGAAACCACCATCCATTAT
GTAGTGAATGGCAATGGCCAGACCTACCATCAACTTGGAGTGTTCATAAATACTCTGAACTTGTTGATAGCAGACTAGGT
AAAATGCTTGATAAAGCTAAAAATTTTGGCTCTGCAACAAAATATCTTGGAAATATAAATGTTCGCTGGTTTAGTTTTGA
TTTAGAAAACCTTCAAGATATACTAATTTCAGATATAGAGCGCAGAGAATTATCACTCAAGCTTGGCGATGTTTTAATAT
GTGAAGGTGGTGAACCTGGTCGATGTGCTATATGGAGTGAACCTCAGGACATACCTGTTATTTTCCAAAAAGCATTACAT
CGAGCACGGGTAAAAGACAAAATCATCCCCGAATGGTTAGTTTATAATTTGAAAAATGATTCAAATAACATTAGCTTATC
TCAGCTCTTTACAGGAACTACAATTAAACACCTTACAGGAAAAGCACTAGCAAATTATCCCATCAGAGTTCCTCCATTAG
AAGAACAACACGAAATCGTCCGCCGCGTCGAACAACTCTTCGCCTGGGCCGACACCATTGAAAAGCAGGTTAACAACGCC
CTGAACCGCGTCAACAGCCTCACCCAGTCAATCCTGGCAAAGGCCTTCCGCGGCGAGCTTACCGCCCAGTGGCGTGCGGA
AAACCCAAGTCTTATCAGCGGTGAAAACAGCGCCGCCGCCCTGCTGGAAAAAATCAAGGCCGAACGCGCCGCCAGCGGTG
GTAAAAAAACCTCGCGTAAAAAAGCCTGA

Upstream 100 bases:

>100_bases
CGCTGGGCGAGCTGGATGCACTGATGCGAGAGCTGGGCGCGGGCGATGAGGCGGATGCGCAGCGCCAGTTGCTGGAAGAA
GCGTTTGGGGGGGAGAAGGA

Downstream 100 bases:

>100_bases
CGCTTATTTTCCTGCGCACCTTCCCGGTGCGCTTTTTATTATTCCCCCGCATTCATAACCATCAAAAATATATCCAGTTT
CTTCCTGAACATTCCCATTA

Product: EcoKI restriction-modification system protein HsdS

Products: NA

Alternate protein names: S.EcoKI; Type I restriction enzyme EcoKI specificity protein; S protein [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSGGKLPEGWVTTHLSEICSKPQYGYTTKSSSMGDVKFLRTTDITKGAVDWSSVPYCMDAPEDVSKYQLQDRDIVISRAG
SVGFSFLVQNPPSQVVFASYLIRFKPVNYFSEYYLKRFLESSDYWNQLSLMSAGNAVQNVNAQKLSTLTVPIPPIAEQKI
IAEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLAAAVSGLLIGSNKRNHHPLCSEWQWPDLPSTWSVHKYSELVDSRLG
KMLDKAKNFGSATKYLGNINVRWFSFDLENLQDILISDIERRELSLKLGDVLICEGGEPGRCAIWSEPQDIPVIFQKALH
RARVKDKIIPEWLVYNLKNDSNNISLSQLFTGTTIKHLTGKALANYPIRVPPLEEQHEIVRRVEQLFAWADTIEKQVNNA
LNRVNSLTQSILAKAFRGELTAQWRAENPSLISGENSAAALLEKIKAERAASGGKKTSRKKA

Sequences:

>Translated_462_residues
MSGGKLPEGWVTTHLSEICSKPQYGYTTKSSSMGDVKFLRTTDITKGAVDWSSVPYCMDAPEDVSKYQLQDRDIVISRAG
SVGFSFLVQNPPSQVVFASYLIRFKPVNYFSEYYLKRFLESSDYWNQLSLMSAGNAVQNVNAQKLSTLTVPIPPIAEQKI
IAEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLAAAVSGLLIGSNKRNHHPLCSEWQWPDLPSTWSVHKYSELVDSRLG
KMLDKAKNFGSATKYLGNINVRWFSFDLENLQDILISDIERRELSLKLGDVLICEGGEPGRCAIWSEPQDIPVIFQKALH
RARVKDKIIPEWLVYNLKNDSNNISLSQLFTGTTIKHLTGKALANYPIRVPPLEEQHEIVRRVEQLFAWADTIEKQVNNA
LNRVNSLTQSILAKAFRGELTAQWRAENPSLISGENSAAALLEKIKAERAASGGKKTSRKKA
>Mature_461_residues
SGGKLPEGWVTTHLSEICSKPQYGYTTKSSSMGDVKFLRTTDITKGAVDWSSVPYCMDAPEDVSKYQLQDRDIVISRAGS
VGFSFLVQNPPSQVVFASYLIRFKPVNYFSEYYLKRFLESSDYWNQLSLMSAGNAVQNVNAQKLSTLTVPIPPIAEQKII
AEKLDTLLAQVDSTKARLEQIPQILKRFRQAVLAAAVSGLLIGSNKRNHHPLCSEWQWPDLPSTWSVHKYSELVDSRLGK
MLDKAKNFGSATKYLGNINVRWFSFDLENLQDILISDIERRELSLKLGDVLICEGGEPGRCAIWSEPQDIPVIFQKALHR
ARVKDKIIPEWLVYNLKNDSNNISLSQLFTGTTIKHLTGKALANYPIRVPPLEEQHEIVRRVEQLFAWADTIEKQVNNAL
NRVNSLTQSILAKAFRGELTAQWRAENPSLISGENSAAALLEKIKAERAASGGKKTSRKKA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

Organism=Escherichia coli, GI1790807, Length=477, Percent_Identity=42.5576519916143, Blast_Score=313, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 51855; Mature: 51724

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGGKLPEGWVTTHLSEICSKPQYGYTTKSSSMGDVKFLRTTDITKGAVDWSSVPYCMDA
CCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
PEDVSKYQLQDRDIVISRAGSVGFSFLVQNPPSQVVFASYLIRFKPVNYFSEYYLKRFLE
CHHHHHHHCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SSDYWNQLSLMSAGNAVQNVNAQKLSTLTVPIPPIAEQKIIAEKLDTLLAQVDSTKARLE
CCCHHHHHHHHHCCCHHHCCCHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QIPQILKRFRQAVLAAAVSGLLIGSNKRNHHPLCSEWQWPDLPSTWSVHKYSELVDSRLG
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KMLDKAKNFGSATKYLGNINVRWFSFDLENLQDILISDIERRELSLKLGDVLICEGGEPG
HHHHHHHCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHEEEECCEEEECCCCCC
RCAIWSEPQDIPVIFQKALHRARVKDKIIPEWLVYNLKNDSNNISLSQLFTGTTIKHLTG
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCEEHHHHHCCCHHHHHHH
KALANYPIRVPPLEEQHEIVRRVEQLFAWADTIEKQVNNALNRVNSLTQSILAKAFRGEL
HHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAQWRAENPSLISGENSAAALLEKIKAERAASGGKKTSRKKA
EEEEECCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SGGKLPEGWVTTHLSEICSKPQYGYTTKSSSMGDVKFLRTTDITKGAVDWSSVPYCMDA
CCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCC
PEDVSKYQLQDRDIVISRAGSVGFSFLVQNPPSQVVFASYLIRFKPVNYFSEYYLKRFLE
CHHHHHHHCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
SSDYWNQLSLMSAGNAVQNVNAQKLSTLTVPIPPIAEQKIIAEKLDTLLAQVDSTKARLE
CCCHHHHHHHHHCCCHHHCCCHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
QIPQILKRFRQAVLAAAVSGLLIGSNKRNHHPLCSEWQWPDLPSTWSVHKYSELVDSRLG
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
KMLDKAKNFGSATKYLGNINVRWFSFDLENLQDILISDIERRELSLKLGDVLICEGGEPG
HHHHHHHCCCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHHEEEECCEEEECCCCCC
RCAIWSEPQDIPVIFQKALHRARVKDKIIPEWLVYNLKNDSNNISLSQLFTGTTIKHLTG
EEEECCCCCCCHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCCEEHHHHHCCCHHHHHHH
KALANYPIRVPPLEEQHEIVRRVEQLFAWADTIEKQVNNALNRVNSLTQSILAKAFRGEL
HHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAQWRAENPSLISGENSAAALLEKIKAERAASGGKKTSRKKA
EEEEECCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6304321; 7610040; 9278503; 7739896 [H]