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The map label for this gene is trmH [H]

Identifier: 29144056

GI number: 29144056

Start: 3896922

End: 3897611

Strand: Reverse

Name: trmH [H]

Synonym: t3775

Alternate gene names: 29144056

Gene position: 3897611-3896922 (Counterclockwise)

Preceding gene: 29144057

Following gene: 29144055

Centisome position: 81.34

GC content: 54.78

Gene sequence:

>690_bases
ATGAATCCAAAACGTTATGCGCGTATATGCGAAATGCTCGCCAGGCGTCAGCCTGATCTCACGGTCTGCATGGAGCAAGT
CCACAAACCTCATAACGTTTCTGCAATCATTCGTACCGCAGATGCCGTTGGCGTCCATGAAGTCCATGCCATCTGGCCTG
GTAGCCGGATGCGCACCATGGCTTCCGCGGCGGCGGGCAGCAACAGTTGGGTACAGGTGAAAACTCACCGTACCATTGAC
GATGCGGTAGCCCATTTGAAAGGACGGGGAATGCAGATTCTGGCCACCCATCTTTCTGATAAGGCCGTAGACTTTCGCGA
GATTGATTACACGCGCCCGACCTGTATTCTGATGGGTCAGGAGAAAACCGGTATTACTCAGGAAGCATTAGACCTGGCGG
ATCGGGACATCATCATTCCGATGATCGGCATGGTACAGTCGCTGAACGTTTCCGTCGCCTCCGCGCTCATTCTTTATGAA
GCCCAGCGCCAGCGACAGAACGCGGGCATGTATCTGCGTGAAAATAGCATGTTGCCGGAAGATGAACAGCAGCGCCTGCT
GTTTGAGGGTGGTTATCCGGTGCTGGCGAAAGTCGCTAAACGTAAAGGTCTGCCTTATCCTCGCGTCAATCAGCAGGGCG
AAATCGACGCCGATGCTGACTGGTGGGCGACGATGCAGGCGGCAGGGTAA

Upstream 100 bases:

>100_bases
CGCCTTACCGCACGCGATCGCGTACATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTTATTAAAGTCAC
CCGTAACCGAAACTAGCGCT

Downstream 100 bases:

>100_bases
TCACCATGTCAGGCCGCTTGTTAGACGCTGTTCCGCTCAATTCCCTGACAGGCGTCGGCGCAGCGCAAAGCAGTAAACTG
GCGAAAATTGGCCTGCATAC

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MNPKRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAIWPGSRMRTMASAAAGSNSWVQVKTHRTID
DAVAHLKGRGMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGITQEALDLADRDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEDEQQRLLFEGGYPVLAKVAKRKGLPYPRVNQQGEIDADADWWATMQAAG

Sequences:

>Translated_229_residues
MNPKRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAIWPGSRMRTMASAAAGSNSWVQVKTHRTID
DAVAHLKGRGMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGITQEALDLADRDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEDEQQRLLFEGGYPVLAKVAKRKGLPYPRVNQQGEIDADADWWATMQAAG
>Mature_229_residues
MNPKRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAIWPGSRMRTMASAAAGSNSWVQVKTHRTID
DAVAHLKGRGMQILATHLSDKAVDFREIDYTRPTCILMGQEKTGITQEALDLADRDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGMYLRENSMLPEDEQQRLLFEGGYPVLAKVAKRKGLPYPRVNQQGEIDADADWWATMQAAG

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=229, Percent_Identity=95.6331877729258, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI1790623, Length=167, Percent_Identity=32.9341317365269, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25606; Mature: 25606

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPKRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAIWPGSRMRTM
CCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEHHHHCCHHHEEEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIDDAVAHLKGRGMQILATHLSDKAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEHHCCCCCCHHHCCCCCCEEEEEEC
EKTGITQEALDLADRDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPE
CCCCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
DEQQRLLFEGGYPVLAKVAKRKGLPYPRVNQQGEIDADADWWATMQAAG
CHHHEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHEEEECCC
>Mature Secondary Structure
MNPKRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAIWPGSRMRTM
CCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHEEEHHHHCCHHHEEEECCCHHHHHH
ASAAAGSNSWVQVKTHRTIDDAVAHLKGRGMQILATHLSDKAVDFREIDYTRPTCILMGQ
HHHHCCCCCEEEEEECCCHHHHHHHHCCCCCEEEEEHHCCCCCCHHHCCCCCCEEEEEEC
EKTGITQEALDLADRDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPE
CCCCCHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
DEQQRLLFEGGYPVLAKVAKRKGLPYPRVNQQGEIDADADWWATMQAAG
CHHHEEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]