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The map label for this gene is rbsR [H]

Identifier: 29143928

GI number: 29143928

Start: 3737153

End: 3738151

Strand: Reverse

Name: rbsR [H]

Synonym: t3633

Alternate gene names: 29143928

Gene position: 3738151-3737153 (Counterclockwise)

Preceding gene: 29143929

Following gene: 29143924

Centisome position: 78.01

GC content: 52.75

Gene sequence:

>999_bases
TTGGCTACTATGAAAGATGTTGCCCGCCTGGCGGGAGTCTCTACATCGACGGTCTCTCACGTTATTAACAAAGATCGCTT
TGTGAGTGAGACGATTACTGAAAAAGTTGAAGCGGCGATTAAATCACTTAACTATGCGCCTTCCGCGCTGGCGCGAAGCC
TCAAGTTAAACCAGACCCGCACGATTGGTATGCTGATTACCGCCAGTACCAACCCTTTTTATTCCGAACTGGTGCGCGGC
GTTGAGCGAAGCTGTTTTGAGCGCGGTTATAGTCTGGTGCTGTGTAATACCGAAGGCGATGAGCAACGGATGAACCGCAA
CCTAGAGACGCTGATGCAAAAACGGGTAGACGGTTTACTGCTGCTGTGTACTGAAACGCATCAGCCTTCTCGTGAAATCA
TGGAACGTTATCCGTCTATTCCTACCGTCATGATGGACTGGTCGCCGTTTGATGGTGAGGGCGATAGCGATCTCATTCAG
GATAATTCGTTGTTAGGCGGCGATCTTGCGACCCAACACCTTATCGATAAAGGATTTACCCGAATAGCCTGTATTACCGG
CCCGCTGGACAAAACGCCTGCGCGTTTGCGGCTGGAAGGGTATCGGGCGGCGATGAAACGCGCAGGGCTGGCTATTCCGC
AAGGCTATGAGATCACCGGCGATTTTGAATTTGGCGGCGGCTTTGACGCCATGCAGGCGTTACTCGCCCATCAACAGCGA
CCGCAAGCGGTTTTTACCGGAAACGATGCGATGGCTGTCGGCGCCTATCAGGCGCTATATCAGGCGGGGCTTCGTATCCC
GCAAGATATGGCAGTCATTGGCTATGATGATATTGAGCTAGCGCGCTATATGACGCCGCCGCTGACGACTATCCATCAGC
CGAAAGATGAATTGGGCGAGCTTGCTATTGATGTGTTGATTCATCGGATGGCGCAGCCCGCCTTGCAACAACAGCGACTT
CAGCTTACTCCGGTTTTGACGGTGCGTGGGTCGGTTTAG

Upstream 100 bases:

>100_bases
CCATGCCGCCGCCGCGATTGCCGTAACGCGTAAAGGCGCGCAACCTTCCGTTCCGTGGCGCGAAGAGATAGAAGCGTTTT
TACATCAGCAGGGGTAACGC

Downstream 100 bases:

>100_bases
ATTTATGCCGTTCTTTAATCAGATTGTTGCCGTCTTTGGCTCTTAGCAGCATAAACATCAGCGCGGACACGATAGTGATC
GCCCCCATCGTGATAAAGGT

Product: transcriptional repressor RbsR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMERYPSIPTVMMDWSPFDGEGDSDLIQ
DNSLLGGDLATQHLIDKGFTRIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDAMQALLAHQQR
PQAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMAQPALQQQRL
QLTPVLTVRGSV

Sequences:

>Translated_332_residues
MATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRG
VERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMERYPSIPTVMMDWSPFDGEGDSDLIQ
DNSLLGGDLATQHLIDKGFTRIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDAMQALLAHQQR
PQAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMAQPALQQQRL
QLTPVLTVRGSV
>Mature_331_residues
ATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLNYAPSALARSLKLNQTRTIGMLITASTNPFYSELVRGV
ERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLLLLCTETHQPSREIMERYPSIPTVMMDWSPFDGEGDSDLIQD
NSLLGGDLATQHLIDKGFTRIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDAMQALLAHQQRP
QAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGELAIDVLIHRMAQPALQQQRLQ
LTPVLTVRGSV

Specific function: Transcriptional repressor for the ribose rbsDACBK operon. RbsR binds to a region of perfect dyad symmetry spanning the rbs operon transcriptional start site. The affinity for the rbs operator is reduced by addition of ribose, consistent with ribose being

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=89.7280966767372, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1787948, Length=335, Percent_Identity=46.2686567164179, Blast_Score=315, Evalue=2e-87,
Organism=Escherichia coli, GI1790369, Length=309, Percent_Identity=35.2750809061489, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=34.0136054421769, Blast_Score=161, Evalue=7e-41,
Organism=Escherichia coli, GI1786540, Length=333, Percent_Identity=32.4324324324324, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1788474, Length=332, Percent_Identity=29.8192771084337, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787580, Length=311, Percent_Identity=29.5819935691318, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1789068, Length=320, Percent_Identity=27.8125, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI48994940, Length=334, Percent_Identity=28.7425149700599, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI1786268, Length=320, Percent_Identity=26.25, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787906, Length=327, Percent_Identity=25.3822629969419, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1790715, Length=311, Percent_Identity=25.4019292604502, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789456, Length=342, Percent_Identity=26.3157894736842, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1790192, Length=250, Percent_Identity=28, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1790689, Length=318, Percent_Identity=25.1572327044025, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36694; Mature: 36562

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLNYAPSALARSLKLNQTR
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMERYPSIPTVMMDWSPFDGEGDSDLIQDNSLLGGDLATQHLIDKGFT
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHCCCCCCCCHHHHHHHHHCCCC
RIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDAMQALLAHQQR
EEEEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCC
PQAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGE
CCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCHHHHHH
LAIDVLIHRMAQPALQQQRLQLTPVLTVRGSV
HHHHHHHHHHHHHHHHHHHHEECEEEEECCCC
>Mature Secondary Structure 
ATMKDVARLAGVSTSTVSHVINKDRFVSETITEKVEAAIKSLNYAPSALARSLKLNQTR
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCE
TIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQRMNRNLETLMQKRVDGLL
EEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHCCHHHHHHHHCCCEE
LLCTETHQPSREIMERYPSIPTVMMDWSPFDGEGDSDLIQDNSLLGGDLATQHLIDKGFT
EEEECCCCCHHHHHHHCCCCCEEEEECCCCCCCCCCCHHCCCCCCCCHHHHHHHHHCCCC
RIACITGPLDKTPARLRLEGYRAAMKRAGLAIPQGYEITGDFEFGGGFDAMQALLAHQQR
EEEEEECCCCCCCHHEEHHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHHHHHHHCCC
PQAVFTGNDAMAVGAYQALYQAGLRIPQDMAVIGYDDIELARYMTPPLTTIHQPKDELGE
CCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCCCCCCCCHHHHHH
LAIDVLIHRMAQPALQQQRLQLTPVLTVRGSV
HHHHHHHHHHHHHHHHHHHHEECEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]