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The map label for this gene is hpcB [H]

Identifier: 29142247

GI number: 29142247

Start: 1878899

End: 1879750

Strand: Reverse

Name: hpcB [H]

Synonym: t1816

Alternate gene names: 29142247

Gene position: 1879750-1878899 (Counterclockwise)

Preceding gene: 29142248

Following gene: 29142246

Centisome position: 39.23

GC content: 54.46

Gene sequence:

>852_bases
ATGGGCAAGTTAGCGTTAGCAGCAAAAATTACCCACGTGCCGTCGATGTATCTTTCTGAACTGCCAGGAAAAAATCACGG
TTGTCGTCAGGCAGCCATTGATGGGCATATTGAAATTGGCAAGCGTTGCCGCGAAATGGGCGTTGACACCATTATCGTAT
TCGACACCCACTGGCTGGTGAATAGCGCTTACCACATTAATTGTGCCGACCATTTCCAGGGCGTCTATACCAGCAACGAA
TTGCCGCACTTTATTCGCGACATGACCTATGACTATGACGGTAATCCGGCGCTCGGCCATCTGATCGCCGACGAGGCGGT
CAAACTGGGCGTGCGCGCCAAAGCGCACAACATCCCGAGCCTGAAGCTGGAGTATGGCACGCTGGTGCCGATGCGCTACA
TGAACAGCGACAAGCACTTCAAAGTGGTCTCCATCTCGGCGTTCTGCACTGTGCATGATTTTGCCGACAGCCGCAAACTG
GGCGAAGCCATTCTCAAGGCGATTGAGAAATATGACGGTACCGTAGCGGTATTCGCCAGTGGTTCTCTGTCGCACCGTTT
TATTGACGACCAACGGGCGGAAGAGGGGATGAACAGCTACACCCGCGAGTTCGATCATCAAATGGACGAGCGCGTGGTCA
AGCTGTGGCGCGAAGGCAAATTCAAGGAGTTTTGCACCATGTTGCCGGAGTACGCCGACTACTGCTACGGCGAAGGCAAC
ATGCACGACACGGTCATGCTACTGGGAATGCTGGGGTGGGACAAATACGACGGCAAGGTGGAGTTCATCACCGACCTGTT
CGCCAGCTCCGGTACCGGCCAGGTAAACGCTGTTTTCCCGCTGCCTGCGTAA

Upstream 100 bases:

>100_bases
GGGCGCGAAGGCGGCGAATATAGCTTCGAAGTGTTTGCGGAAATGAAAAACGTCTGCATCTCAATGGGCGACCATCCTAT
CCCAAAATGGGGAGTTTGAT

Downstream 100 bases:

>100_bases
GGGGGGTTTATGCCGCACTTTATTGCTGAATGTACTGAAAATATTCGCGAGCAGGCTGATTTACCAAGCCTGTTCAGCAA
GGTAAACGAGGCGCTGGCCG

Product: 3,4-dihydroxyphenylacetate 2,3-dioxygenase

Products: NA

Alternate protein names: Homoprotocatechuate 2,3-dioxygenase; HPC dioxygenase [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MGKLALAAKITHVPSMYLSELPGKNHGCRQAAIDGHIEIGKRCREMGVDTIIVFDTHWLVNSAYHINCADHFQGVYTSNE
LPHFIRDMTYDYDGNPALGHLIADEAVKLGVRAKAHNIPSLKLEYGTLVPMRYMNSDKHFKVVSISAFCTVHDFADSRKL
GEAILKAIEKYDGTVAVFASGSLSHRFIDDQRAEEGMNSYTREFDHQMDERVVKLWREGKFKEFCTMLPEYADYCYGEGN
MHDTVMLLGMLGWDKYDGKVEFITDLFASSGTGQVNAVFPLPA

Sequences:

>Translated_283_residues
MGKLALAAKITHVPSMYLSELPGKNHGCRQAAIDGHIEIGKRCREMGVDTIIVFDTHWLVNSAYHINCADHFQGVYTSNE
LPHFIRDMTYDYDGNPALGHLIADEAVKLGVRAKAHNIPSLKLEYGTLVPMRYMNSDKHFKVVSISAFCTVHDFADSRKL
GEAILKAIEKYDGTVAVFASGSLSHRFIDDQRAEEGMNSYTREFDHQMDERVVKLWREGKFKEFCTMLPEYADYCYGEGN
MHDTVMLLGMLGWDKYDGKVEFITDLFASSGTGQVNAVFPLPA
>Mature_282_residues
GKLALAAKITHVPSMYLSELPGKNHGCRQAAIDGHIEIGKRCREMGVDTIIVFDTHWLVNSAYHINCADHFQGVYTSNEL
PHFIRDMTYDYDGNPALGHLIADEAVKLGVRAKAHNIPSLKLEYGTLVPMRYMNSDKHFKVVSISAFCTVHDFADSRKLG
EAILKAIEKYDGTVAVFASGSLSHRFIDDQRAEEGMNSYTREFDHQMDERVVKLWREGKFKEFCTMLPEYADYCYGEGNM
HDTVMLLGMLGWDKYDGKVEFITDLFASSGTGQVNAVFPLPA

Specific function: Transforms homoprotocatechuic acid (HPC) into 5- carboxymethyl-2-hydroxy-muconic semialdehyde (CHMS) [H]

COG id: COG3384

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011984
- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.15 [H]

Molecular weight: Translated: 31831; Mature: 31699

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLALAAKITHVPSMYLSELPGKNHGCRQAAIDGHIEIGKRCREMGVDTIIVFDTHWLV
CCCHHHHHHHHHCHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHCCCCEEEEEECHHHH
NSAYHINCADHFQGVYTSNELPHFIRDMTYDYDGNPALGHLIADEAVKLGVRAKAHNIPS
CCEEEEEHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCC
LKLEYGTLVPMRYMNSDKHFKVVSISAFCTVHDFADSRKLGEAILKAIEKYDGTVAVFAS
EEEECCCCCCHHHCCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
GSLSHRFIDDQRAEEGMNSYTREFDHQMDERVVKLWREGKFKEFCTMLPEYADYCYGEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
MHDTVMLLGMLGWDKYDGKVEFITDLFASSGTGQVNAVFPLPA
CHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCC
>Mature Secondary Structure 
GKLALAAKITHVPSMYLSELPGKNHGCRQAAIDGHIEIGKRCREMGVDTIIVFDTHWLV
CCHHHHHHHHHCHHHHHHHCCCCCCCCCHHHCCCHHHHHHHHHHCCCCEEEEEECHHHH
NSAYHINCADHFQGVYTSNELPHFIRDMTYDYDGNPALGHLIADEAVKLGVRAKAHNIPS
CCEEEEEHHHHHCCCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCCC
LKLEYGTLVPMRYMNSDKHFKVVSISAFCTVHDFADSRKLGEAILKAIEKYDGTVAVFAS
EEEECCCCCCHHHCCCCCCEEEEEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEC
GSLSHRFIDDQRAEEGMNSYTREFDHQMDERVVKLWREGKFKEFCTMLPEYADYCYGEGN
CCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCC
MHDTVMLLGMLGWDKYDGKVEFITDLFASSGTGQVNAVFPLPA
CHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2261999 [H]