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The map label for this gene is rutR [H]

Identifier: 29142230

GI number: 29142230

Start: 1863577

End: 1864215

Strand: Reverse

Name: rutR [H]

Synonym: t1799

Alternate gene names: 29142230

Gene position: 1864215-1863577 (Counterclockwise)

Preceding gene: 29142231

Following gene: 29142227

Centisome position: 38.9

GC content: 54.3

Gene sequence:

>639_bases
ATGTCGCAACGCACAGAGAAAAAAATCGGGAAACGTTCGCAGGCCACCGGTGCAAAACGGCAGCTTATCTTAACCGCCGC
GCTTGCCGTTTTTTCCCAGTATGGCATTCATGGCGCGCGTCTTGAACAGGTCGCCGAGCGGGCAGGCGTCTCCAAAACCA
ATCTGCTTTATTATTATCCCTCGAAAGAGGCGCTGTATGTCGCGGTAATGCGACAGATTCTGGATGTCTGGTTGGCGCCG
CTCAAGGCGTTTCGCGCAGAATTTTCCCCTCTGGAGGCCATCAAAGAGTATATCCGTCTCAAGCTGGAGGTTTCGCGTGA
TTATCCGCAGGCGTCGCGGCTCTTCTGCATGGAGATGCTGGCGGGCGCGCCGCTCTTAATGGATGAACTGACCGGCGATC
TAAAAGCGTTGATAGATGAAAAATCCGCGCTGATTGCCGGATGGGTGCACAGCGGGAAACTCGCGCCCGTTTCTCCGCAT
CATTTGATCTTCATGATTTGGGCCGCCACGCAACATTACGCCGATTTCGCCCCTCAGGTTGAAGCGGTAACCGGCGCGAC
GCTTCGCGATGAAGCCTTTTTCAACCAAACGGTCGAAAGCGTTCAGCGCATTATTATTGAAGGGATTCGCGTGCGTTAA

Upstream 100 bases:

>100_bases
CTAAAAAGCGTCATGCGCCACCGCCGCCGGATTTCCGGCGGTTTTTTTGTTTATAAGCCGGATTTGGTATGCTTGCGTCC
TGACGAAAAGGAGAGGGCGC

Downstream 100 bases:

>100_bases
CCGGCTGGCGGCAGTGGGCAGCTCAAATCACCCTCTTCACATTGCAGCAGCGAGGCTAAAAACGCTTCGCGTTCGTTCGT
TTTATCGGTCAGGCACTGGC

Product: transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 212; Mature: 211

Protein sequence:

>212_residues
MSQRTEKKIGKRSQATGAKRQLILTAALAVFSQYGIHGARLEQVAERAGVSKTNLLYYYPSKEALYVAVMRQILDVWLAP
LKAFRAEFSPLEAIKEYIRLKLEVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVHSGKLAPVSPH
HLIFMIWAATQHYADFAPQVEAVTGATLRDEAFFNQTVESVQRIIIEGIRVR

Sequences:

>Translated_212_residues
MSQRTEKKIGKRSQATGAKRQLILTAALAVFSQYGIHGARLEQVAERAGVSKTNLLYYYPSKEALYVAVMRQILDVWLAP
LKAFRAEFSPLEAIKEYIRLKLEVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVHSGKLAPVSPH
HLIFMIWAATQHYADFAPQVEAVTGATLRDEAFFNQTVESVQRIIIEGIRVR
>Mature_211_residues
SQRTEKKIGKRSQATGAKRQLILTAALAVFSQYGIHGARLEQVAERAGVSKTNLLYYYPSKEALYVAVMRQILDVWLAPL
KAFRAEFSPLEAIKEYIRLKLEVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVHSGKLAPVSPHH
LIFMIWAATQHYADFAPQVEAVTGATLRDEAFFNQTVESVQRIIIEGIRVR

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=212, Percent_Identity=83.0188679245283, Blast_Score=371, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 23778; Mature: 23647

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRTEKKIGKRSQATGAKRQLILTAALAVFSQYGIHGARLEQVAERAGVSKTNLLYYYP
CCCHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEC
SKEALYVAVMRQILDVWLAPLKAFRAEFSPLEAIKEYIRLKLEVSRDYPQASRLFCMEML
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AGAPLLMDELTGDLKALIDEKSALIAGWVHSGKLAPVSPHHLIFMIWAATQHYADFAPQV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCH
EAVTGATLRDEAFFNQTVESVQRIIIEGIRVR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SQRTEKKIGKRSQATGAKRQLILTAALAVFSQYGIHGARLEQVAERAGVSKTNLLYYYP
CCHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEEEC
SKEALYVAVMRQILDVWLAPLKAFRAEFSPLEAIKEYIRLKLEVSRDYPQASRLFCMEML
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
AGAPLLMDELTGDLKALIDEKSALIAGWVHSGKLAPVSPHHLIFMIWAATQHYADFAPQV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHHHHHHHCCCH
EAVTGATLRDEAFFNQTVESVQRIIIEGIRVR
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]