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The map label for this gene is pspA [H]

Identifier: 29142035

GI number: 29142035

Start: 1653140

End: 1653808

Strand: Reverse

Name: pspA [H]

Synonym: t1595

Alternate gene names: 29142035

Gene position: 1653808-1653140 (Counterclockwise)

Preceding gene: 29142039

Following gene: 29142034

Centisome position: 34.51

GC content: 51.27

Gene sequence:

>669_bases
ATGGGTATTTTTTCTCGTTTTGCCGACATCGTGAACGCCAATATCAATGCGTTGTTGGAAAAAGCGGAAGATCCGCAGAA
GCTGGTGCGCCTGATGATTCAGGAGATGGAAGATACGCTAGTGGAGGTTCGCTCGAACTCCGCGCGAGCTTTAGCGGAAA
AGAAACAGTTATCCCGTCGTATTGAACAGGCTACGGCTCAGCAGACCGAGTGGCAGGAAAAAGCGGAACTGGCTCTGCGC
AAAGATAAAGACGATCTGGCGCGCGCCGCATTGATTGAAAAACAAAAGCTGACCGATTTAATTGCTACGCTTGAACAGGA
AGTCACGCTGGTGGATGACACGCTGGCGCGCATGAAGAAAGAGATTGGCGAGCTGGAAAACAAACTCAGCGAAACACGCG
CTCGCCAGCAGGCGCTGATGCTGCGTCATCAGGCGGCAAGTTCTTCCCGCGATGTCCGTCGTCAGCTTGACAGCGGCAAA
CTGGATGAAGCCATGGCGCGGTTTGAATCCTTTGAACGTCGTATCGATCAAATGGAAGCGGAAGCGGAAAGCCATCGTTT
TGGTAAGCAGCAATCGCTGGACCAACAGTTTGCCAACCTGAAAGCCGATGATGAAATCAGCGAGCAGCTGGCGCAGTTGA
AAGCCAAAATGAAGCAAGATAATCAATAA

Upstream 100 bases:

>100_bases
ATTCCTTAACTATCAATAATATAAAAAGTTGGCACGCAAATTGTATTAATCATGTAGCAGGGCATCGCCCGTTATCAGAA
CATTATGTGAGGATTGAATT

Downstream 100 bases:

>100_bases
TGACAATGGCGGCGCCGGTACGCGCCGTCGCTCATCAGCTTTAGGTGTAAGGAGAACGCATGAGCGCGCTATTTCTGGCC
ATCCCGTTAACCATTTTTGT

Product: phage shock protein PspA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSNSARALAEKKQLSRRIEQATAQQTEWQEKAELALR
KDKDDLARAALIEKQKLTDLIATLEQEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAASSSRDVRRQLDSGK
LDEAMARFESFERRIDQMEAEAESHRFGKQQSLDQQFANLKADDEISEQLAQLKAKMKQDNQ

Sequences:

>Translated_222_residues
MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSNSARALAEKKQLSRRIEQATAQQTEWQEKAELALR
KDKDDLARAALIEKQKLTDLIATLEQEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAASSSRDVRRQLDSGK
LDEAMARFESFERRIDQMEAEAESHRFGKQQSLDQQFANLKADDEISEQLAQLKAKMKQDNQ
>Mature_221_residues
GIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSNSARALAEKKQLSRRIEQATAQQTEWQEKAELALRK
DKDDLARAALIEKQKLTDLIATLEQEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAASSSRDVRRQLDSGKL
DEAMARFESFERRIDQMEAEAESHRFGKQQSLDQQFANLKADDEISEQLAQLKAKMKQDNQ

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspA negatively regulates expression of the pspABCDE promoter and of pspG through negative regulation

COG id: COG1842

COG function: function code KT; Phage shock protein A (IM30), suppresses sigma54-dependent transcription

Gene ontology:

Cell location: Cytoplasm. Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PspA/IM30 family [H]

Homologues:

Organism=Escherichia coli, GI2367118, Length=222, Percent_Identity=91.4414414414414, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014319
- InterPro:   IPR007157 [H]

Pfam domain/function: PF04012 PspA_IM30 [H]

EC number: NA

Molecular weight: Translated: 25512; Mature: 25381

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSNSARALAEKKQLSRR
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IEQATAQQTEWQEKAELALRKDKDDLARAALIEKQKLTDLIATLEQEVTLVDDTLARMKK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGELENKLSETRARQQALMLRHQAASSSRDVRRQLDSGKLDEAMARFESFERRIDQMEA
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EAESHRFGKQQSLDQQFANLKADDEISEQLAQLKAKMKQDNQ
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVRSNSARALAEKKQLSRR
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
IEQATAQQTEWQEKAELALRKDKDDLARAALIEKQKLTDLIATLEQEVTLVDDTLARMKK
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGELENKLSETRARQQALMLRHQAASSSRDVRRQLDSGKLDEAMARFESFERRIDQMEA
HHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
EAESHRFGKQQSLDQQFANLKADDEISEQLAQLKAKMKQDNQ
HHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]