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The map label for this gene is ycjG [H]

Identifier: 29142025

GI number: 29142025

Start: 1645181

End: 1646146

Strand: Reverse

Name: ycjG [H]

Synonym: t1585

Alternate gene names: 29142025

Gene position: 1646146-1645181 (Counterclockwise)

Preceding gene: 29142028

Following gene: 29142023

Centisome position: 34.35

GC content: 57.45

Gene sequence:

>966_bases
ATGAGATCTGTTAAGGTATATGAGGAAACCTGGCCGTTACATACGCCTTTTGTTATTGCGCGTGGTAGCCGAAGCGAAGC
GCATGTTGTCGTTGTCGAACTGGAAGAAGAGGGCGTAAAAGGCATCGGGGAATGTACGCCATATCCACGTTATGGTGAAA
GTGATGCCTCGGTCATGGCGCAAATCATGAGTATTGTGCCTCAGTTGGAAAACGGGCTGACTCGCGAAGCATTGCAGAAA
CTGTTGCCGGCAGGCGCGGCGCGCAACGCGGTGGATAGCGCCTTATGGGATTTGCAGGCCCGCCAGCGGCAGCAAAGCCT
TGCCGGGCTGCTCGGCGTGACGCCGTCCCCGGTTTTTACTACCGCGCAAACGGTAGTGATAGGCACGCCGGAACAGATGG
CTGCCAGTGCGGCGGCACTATGGAAAAAAGGGGCGACGTTGCTGAAAATTAAACTCGATGCTCGTCTGATTAGCGAACGT
ATGGTGGCGATCCGCTCGGCGGTGCCGGAGGCGACGCTCATTGTGGACGCCAATGAATCGTGGCGCCCAGAAGGGCTGGC
GGCGCGTTGCCAGTTACTGGCGGATCTGAATGTCGCGATGCTGGAGCAACCGCTGCCCGCGCAGGATGACGCCGCCCTGG
AAAATTTTATTCATCCGTTGCCCATCTGTGCAGATGAGAGCTGCCATACTCGCAGTAGTCTGAAAGCCCTGACGGGGCGC
TACGATATGATCAACATTAAGCTGGATAAAACCGGCGGTCTGACTGAGGCGCTGGCGCTGGCAACGGAGGCGCGGGAGCA
AGGATTCGGCCTGATGCTGGGCTGTATGCTCTGTACATCAAGAGCGATTAGCGCCGCGCTGCCGCTTATGCCGCAGGTGA
GCTTCGCCGATCTTGATGGCCCAACATGGCTGGCGGTTGATGTTGAGCCTGCGCTACGCTTTACGACGGGTCAACTCCAT
CTTTAG

Upstream 100 bases:

>100_bases
TACTTATAGTTAACGTCGAAATCCAGTTTAAGTCATTATTCATTAACACGGCAAATACTATTTGTAGCAAGCCGCGTCCG
GCGCTATCAGAGGGGAAGAG

Downstream 100 bases:

>100_bases
GATGCCAGCGGAGCAGATTCGACATCGCCAGCAAGTATTTTTCGCTGGCTTCATCGGCGGAAACAGGGGGAAACTCGGCG
GTAATGCAGTGCAGGTTGAG

Product: mandelate racemase

Products: NA

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MRSVKVYEETWPLHTPFVIARGSRSEAHVVVVELEEEGVKGIGECTPYPRYGESDASVMAQIMSIVPQLENGLTREALQK
LLPAGAARNAVDSALWDLQARQRQQSLAGLLGVTPSPVFTTAQTVVIGTPEQMAASAAALWKKGATLLKIKLDARLISER
MVAIRSAVPEATLIVDANESWRPEGLAARCQLLADLNVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSSLKALTGR
YDMINIKLDKTGGLTEALALATEAREQGFGLMLGCMLCTSRAISAALPLMPQVSFADLDGPTWLAVDVEPALRFTTGQLH
L

Sequences:

>Translated_321_residues
MRSVKVYEETWPLHTPFVIARGSRSEAHVVVVELEEEGVKGIGECTPYPRYGESDASVMAQIMSIVPQLENGLTREALQK
LLPAGAARNAVDSALWDLQARQRQQSLAGLLGVTPSPVFTTAQTVVIGTPEQMAASAAALWKKGATLLKIKLDARLISER
MVAIRSAVPEATLIVDANESWRPEGLAARCQLLADLNVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSSLKALTGR
YDMINIKLDKTGGLTEALALATEAREQGFGLMLGCMLCTSRAISAALPLMPQVSFADLDGPTWLAVDVEPALRFTTGQLH
L
>Mature_321_residues
MRSVKVYEETWPLHTPFVIARGSRSEAHVVVVELEEEGVKGIGECTPYPRYGESDASVMAQIMSIVPQLENGLTREALQK
LLPAGAARNAVDSALWDLQARQRQQSLAGLLGVTPSPVFTTAQTVVIGTPEQMAASAAALWKKGATLLKIKLDARLISER
MVAIRSAVPEATLIVDANESWRPEGLAARCQLLADLNVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSSLKALTGR
YDMINIKLDKTGGLTEALALATEAREQGFGLMLGCMLCTSRAISAALPLMPQVSFADLDGPTWLAVDVEPALRFTTGQLH
L

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=321, Percent_Identity=85.3582554517134, Blast_Score=546, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 34619; Mature: 34619

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSVKVYEETWPLHTPFVIARGSRSEAHVVVVELEEEGVKGIGECTPYPRYGESDASVMA
CCCCEEEHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
QIMSIVPQLENGLTREALQKLLPAGAARNAVDSALWDLQARQRQQSLAGLLGVTPSPVFT
HHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TAQTVVIGTPEQMAASAAALWKKGATLLKIKLDARLISERMVAIRSAVPEATLIVDANES
CCCEEEEECHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCC
WRPEGLAARCQLLADLNVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSSLKALTGR
CCCCCHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
YDMINIKLDKTGGLTEALALATEAREQGFGLMLGCMLCTSRAISAALPLMPQVSFADLDG
EEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
PTWLAVDVEPALRFTTGQLHL
CEEEEEECCCCCEEECCCCCC
>Mature Secondary Structure
MRSVKVYEETWPLHTPFVIARGSRSEAHVVVVELEEEGVKGIGECTPYPRYGESDASVMA
CCCCEEEHHCCCCCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHH
QIMSIVPQLENGLTREALQKLLPAGAARNAVDSALWDLQARQRQQSLAGLLGVTPSPVFT
HHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
TAQTVVIGTPEQMAASAAALWKKGATLLKIKLDARLISERMVAIRSAVPEATLIVDANES
CCCEEEEECHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCC
WRPEGLAARCQLLADLNVAMLEQPLPAQDDAALENFIHPLPICADESCHTRSSLKALTGR
CCCCCHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCC
YDMINIKLDKTGGLTEALALATEAREQGFGLMLGCMLCTSRAISAALPLMPQVSFADLDG
EEEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCC
PTWLAVDVEPALRFTTGQLHL
CEEEEEECCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]