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The map label for this gene is narY [H]

Identifier: 29141934

GI number: 29141934

Start: 1540798

End: 1542342

Strand: Direct

Name: narY [H]

Synonym: t1489

Alternate gene names: 29141934

Gene position: 1540798-1542342 (Clockwise)

Preceding gene: 29141933

Following gene: 29141935

Centisome position: 32.15

GC content: 54.11

Gene sequence:

>1545_bases
ATGAAAATACGCTCACAGGTTGGGATGGTACTGAATCTCGATAAATGTATTGGCTGTCACACCTGCTCCGTCACCTGTAA
AAACGTCTGGACCGGACGTGAAGGGATGGAATACGCATGGTTCAATAACGTCGAAACTAAACCGGGTATTGGCTATCCCA
AAAATTGGGAAGACCAGCAGGAGTGGCAAGGCGGTTGGGTTCGTGATGTTAATGGCAAGATAAGGCCCCGTCTGGGCGGC
AAAATGGGGGTAATTAGCAAAATTTTCGCCAACCCGGTGATTCCGCAAATCGATGATTACTATGAACCCTTTACCTTCGA
TTATCAGCATTTACATACAGCGCCGGAGAGTAAGCATCAGCCCACTGCCCGTCCGCGTTCACTGATCGACGGAAAACGGA
TGGACAAGGTGATCTGGGGGCCAAACTGGGAAGAGCTGCTCGGCGGCGAGTTTGAAAAACGCGCCCGCGACCGCAACTTC
GACAACATACAAAAGGAGATGTACGGTCAGTTCGAAAACACCTTCATGATGTACCTGCCGCGCCTGTGCGAACACTGCCT
GAACCCCAGTTGTGTTGCCACCTGCCCAAGTGGCGCTATTTATAAGCGTGAAGAAGACGGCATCGTGCTAATCGACCAGG
ATAAATGCCGGGGCTGGCGTTTGTGTATCAGCGGCTGCCCATACAAAAAAATCTACTTTAACTGGAAGAGCGGCAAGTCT
GAGAAATGCATATTTTGCTATCCGCGTATTGAATCCGGCCAGCCGACCGTTTGCTCGGAAACCTGCGTCGGGCGCATCCG
TTATCTTGGCGTTCTGCTCTACGACGCCGACAGGATTGAAGAAGCGGCCAGTACCGAACACGAGACAGATTTGTATGAAC
GTCAGTGCGATGTGTTTCTCAATCCGCACGATCCGGCGGTCATCGAAGAGGCGCTTAAACAGGGCATCCCGCAAAACGTC
ATCGACGCCGCCCAACGGTCGCCGGTGTACAAAATGGCGATGGACTGGAAGCTGGCGCTTCCGCTGCACCCGGAATACCG
TACCCTGCCGATGGTCTGGTACGTACCGCCGCTATCGCCCATCCAGTCATACGCCGACGCGGGCGGGTTGCCGCACAATG
GCAATATTCTGCCGGCGGTGGAGACGCTGCGTATTCCGGTCCAGTACCTGGCCAATATGCTCAGCGCGGGCGATACCGGC
CCGGTCATTCGGGCGCTAAAGCGGATGATGGCGATGCGCCACTACATGCGCTCGCAGACGGTTGAAGGCGTGACGGATAC
CCGCGCCATTGACGAAGTCGGTCTGAGCGTACAACAGGTTGAAGAGATGTATCGCTACCTGGCGATTGCCAATTATGAAG
ATCGTTTTGTCATTCCCACCAGCCACCGGGAAATGGCGCGCGATGCCTTTCCGGAGCGCAACGGCTGCGGCTTTACTTTT
GGCGACGGTTGTCACGGCTCGGATACCAAATTCAACCTGTTCAACAGTAGCCGTATTGATGCCATCAATATCACCGAAGT
ACGTGATAAAGCGGAGGGGGAATAA

Upstream 100 bases:

>100_bases
CCGTGGGTTCGAATCGCGATGAATTCATCATGATCAGGAAGATGAAAAACATTAACTGGCTGGATGATGAGGGCCGGGAT
CAGGTACAGGAGGCGAAAAA

Downstream 100 bases:

>100_bases
TGCAAATCCTCAAAGCGATTGGCCTGTTGATGGAGTATCCGGACGACGAGTTGTGGGAATGTCGGGATGAAGCGCTTGCC
CTAATCCAACACGATGCGCC

Product: respiratory nitrate reductase 2 subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 514; Mature: 514

Protein sequence:

>514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQEWQGGWVRDVNGKIRPRLGG
KMGVISKIFANPVIPQIDDYYEPFTFDYQHLHTAPESKHQPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
DNIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFLNPHDPAVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTG
PVIRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE

Sequences:

>Translated_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQEWQGGWVRDVNGKIRPRLGG
KMGVISKIFANPVIPQIDDYYEPFTFDYQHLHTAPESKHQPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
DNIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFLNPHDPAVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTG
PVIRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE
>Mature_514_residues
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQEWQGGWVRDVNGKIRPRLGG
KMGVISKIFANPVIPQIDDYYEPFTFDYQHLHTAPESKHQPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
DNIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFLNPHDPAVIEEALKQGIPQNV
IDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTG
PVIRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in t

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787740, Length=514, Percent_Identity=94.5525291828794, Blast_Score=1031, Evalue=0.0,
Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=80.2371541501976, Blast_Score=878, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=99, Percent_Identity=42.4242424242424, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787122, Length=99, Percent_Identity=42.4242424242424, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI2367345, Length=111, Percent_Identity=30.6306306306306, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789370, Length=99, Percent_Identity=37.3737373737374, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=36.046511627907, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=34.4827586206897, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58753; Mature: 58753

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQ
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGGKMGVISKIFANPVIPQIDDYYEPFTFDYQHLHTAPESKHQ
HHCCCCEEECCCEECCCCCCCHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFDNIQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHCCCEEE
NPHDPAVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTGPVIRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIDEVGLSVQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCCC
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCEEEEECCCCCCEEEHHHHHHHCCCC
>Mature Secondary Structure
MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNVETKPGIGYPKNWEDQQ
CCCHHHCCCEEEHHHHHCCCEEEEEHHHCCCCCCCCCEEECCCCCCCCCCCCCCCCCCHH
EWQGGWVRDVNGKIRPRLGGKMGVISKIFANPVIPQIDDYYEPFTFDYQHLHTAPESKHQ
HHCCCCEEECCCEECCCCCCCHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHCCCCCCCC
PTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNFDNIQKEMYGQFENTFMMYLP
CCCCCHHHHCCHHHCCEEECCCHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRGWRLCISGCPYKKIYFNWKSGKS
HHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCCCCEEEEECCCCCEEEEEEECCCCC
EKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDADRIEEAASTEHETDLYERQCDVFL
CCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHEECHHHHHHHHCCCHHHHHHHHCCCEEE
NPHDPAVIEEALKQGIPQNVIDAAQRSPVYKMAMDWKLALPLHPEYRTLPMVWYVPPLSP
CCCCHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECEEEECCCCCCCCCCCEEEECCCCHH
IQSYADAGGLPHNGNILPAVETLRIPVQYLANMLSAGDTGPVIRALKRMMAMRHYMRSQT
HHHHHHCCCCCCCCCCCCCHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
VEGVTDTRAIDEVGLSVQQVEEMYRYLAIANYEDRFVIPTSHREMARDAFPERNGCGFTF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECCCHHHHHHHCCCCCCCCCCCC
GDGCHGSDTKFNLFNSSRIDAINITEVRDKAEGE
CCCCCCCCCEEEEECCCCCCEEEHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2233673; 9097039; 9278503 [H]