Click here to switch to the map view.

The map label for this gene is narU [H]

Identifier: 29141932

GI number: 29141932

Start: 1535593

End: 1536981

Strand: Direct

Name: narU [H]

Synonym: t1487

Alternate gene names: 29141932

Gene position: 1535593-1536981 (Clockwise)

Preceding gene: 29141931

Following gene: 29141933

Centisome position: 32.05

GC content: 52.12

Gene sequence:

>1389_bases
ATGACACGACAAAACGAGAATTATAACCGTTATCTCTTGAGTGACTGGCGACCCGAAAATCCAGCCTTCTGGGAGAATAA
AGGGAAAGGCATCGCCAGAAGAAACTTATGGATTTCAGTAAGTTGTTTGCTCCTTGCATTTTGCGTCTGGATGTTGTTTA
GCGCTGTTGCCGTCAACCTCAATAAAATTGGATTTGATTTTACGACCGACCAACTCTTTTTATTAACCGCATTACCTTCT
CTTTCCGGCGCAATATTACGCGTTCCCTACTCCTTTATGGTACCACTGTTTGGTGGTCGTAAATGGACCGTATTAAGTAC
CGTAATATTGATTATTCCTTGCGCCTGGCTGGGATTCGCCGTGCAAAATCCTGCCACCCCTTTCGGGGTATTTATACTCA
TCGCTCTGTTGTGCGGTTTTGCGGGGGCAAATTTCGCGTCCAGTATGGGCAACATCAGTTTTTTCTTTCCAAAAGCCAGA
CAAGGTAGCGCGTTGGGTATTAACGACGGACTGGGAAATCTTGGCGTCAGTGTGATGCAGTTGATTGCGCCGCTGGTTAT
TTTTTTGCCGATATTTACTTTTTTAGGCGTCCGGGGCGTGCCGCAACCTGATGGCTCCCTGCTCGCCCTCACTAACGCCG
CCTGGATCTGGGTGCCGTTACTGGCTGTTGCGACGCTTGCCGCCTGGTTTGGTATGAACGATATCGGCAGTTCAAAAGCG
TCTGTCGCCTCGCAATTGCCGGTGCTAAAACGTCTGCACCTGTGGCTGCTTAGCCTGCTGTACCTGGCGACATTCGGGTC
ATTCATCGGCTTTTCCGCAGGTTTCGCCATGCTCGCCAAAACGCAATTTCCCGACGTAAACATTCTGCAACTGGCGTTCT
TTGGCCCCTTTATCGGCGCGCTGGCGCGATCGGCAGGCGGCGTGATCTCCGATAAATTCGGCGGCGTTCGCGTCACGCTC
ATTAATTTTATCTTTATGGCGCTGTTTACCGCCCTGCTATTCCTCACGCTGCCAGGCTCCGGCGCAGGCAGCTTTAGCGC
GTTCTATCTTGTTTTTATGGGCCTGTTTTTGACAGCGGGTCTCGGCAGCGGTTCTACCTTCCAGATGATTGCGGTGATCT
TCCGGCAGATCACGCTCTACAACGTCAAACTGCGCGGCGGGAGCGATGAGCAGGCGCAGCGCGAAGCGGTGACGGATACC
GCCGCCGCGCTCGGTTTTATCTCCGCTATTGGCGCTGTGGGCGGCTTCTTTATTCCAAAAGCGTTTGGCACCTCTTTGGC
CTTGACCGGCTCGCCGGTGGGCGCCATGAAAATCTTTTTGCTGTTTTACATCGCTTGCGTCCTGCTGACCTGGCTGGTCT
ACGGGCGTCTTAAGCCTAAACAACAATAA

Upstream 100 bases:

>100_bases
AATATTCTAAAAAAACTTCAATAAGTTCTCAAAAGAGCGCGTGACGCTCTGTCTCTTTTTTGTGTTTTTTTTCAAATGAT
ATGCGCATGTGAGGGGTAAA

Downstream 100 bases:

>100_bases
CAACATACGCACTTGTAGGACGGCCATCCGGTAATGGTTTAACGCCAAATCGACAGGATGGCGGAAAATTTATCGAAGCA
GGAGAAATGTCATGAGTAAA

Product: nitrite extrusion protein

Products: Proton [Cytoplasm]; nitrite [Periplasm] [C]

Alternate protein names: Nitrite facilitator 2 [H]

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNLNKIGFDFTTDQLFLLTALPS
LSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFAVQNPATPFGVFILIALLCGFAGANFASSMGNISFFFPKAR
QGSALGINDGLGNLGVSVMQLIAPLVIFLPIFTFLGVRGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIGSSKA
SVASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGALARSAGGVISDKFGGVRVTL
INFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDT
AAALGFISAIGAVGGFFIPKAFGTSLALTGSPVGAMKIFLLFYIACVLLTWLVYGRLKPKQQ

Sequences:

>Translated_462_residues
MTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNLNKIGFDFTTDQLFLLTALPS
LSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFAVQNPATPFGVFILIALLCGFAGANFASSMGNISFFFPKAR
QGSALGINDGLGNLGVSVMQLIAPLVIFLPIFTFLGVRGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIGSSKA
SVASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGALARSAGGVISDKFGGVRVTL
INFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDT
AAALGFISAIGAVGGFFIPKAFGTSLALTGSPVGAMKIFLLFYIACVLLTWLVYGRLKPKQQ
>Mature_461_residues
TRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNLNKIGFDFTTDQLFLLTALPSL
SGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFAVQNPATPFGVFILIALLCGFAGANFASSMGNISFFFPKARQ
GSALGINDGLGNLGVSVMQLIAPLVIFLPIFTFLGVRGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIGSSKAS
VASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGALARSAGGVISDKFGGVRVTLI
NFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAGLGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDTA
AALGFISAIGAVGGFFIPKAFGTSLALTGSPVGAMKIFLLFYIACVLLTWLVYGRLKPKQQ

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787743, Length=462, Percent_Identity=84.8484848484848, Blast_Score=749, Evalue=0.0,
Organism=Escherichia coli, GI1787475, Length=456, Percent_Identity=75.219298245614, Blast_Score=676, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49778; Mature: 49646

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNL
CCCCCCCCCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKIGFDFTTDQLFLLTALPSLSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFA
HHCCCCCCCCHHHHHHHHCCCCCHHEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VQNPATPFGVFILIALLCGFAGANFASSMGNISFFFPKARQGSALGINDGLGNLGVSVMQ
HCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
LIAPLVIFLPIFTFLGVRGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIGSSKA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
SVASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LARSAGGVISDKFGGVRVTLINFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAG
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
LGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDTAAALGFISAIGAVGGFFIPK
CCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGTSLALTGSPVGAMKIFLLFYIACVLLTWLVYGRLKPKQQ
HHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TRQNENYNRYLLSDWRPENPAFWENKGKGIARRNLWISVSCLLLAFCVWMLFSAVAVNL
CCCCCCCCCEEECCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
NKIGFDFTTDQLFLLTALPSLSGAILRVPYSFMVPLFGGRKWTVLSTVILIIPCAWLGFA
HHCCCCCCCCHHHHHHHHCCCCCHHEECCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
VQNPATPFGVFILIALLCGFAGANFASSMGNISFFFPKARQGSALGINDGLGNLGVSVMQ
HCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHH
LIAPLVIFLPIFTFLGVRGVPQPDGSLLALTNAAWIWVPLLAVATLAAWFGMNDIGSSKA
HHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
SVASQLPVLKRLHLWLLSLLYLATFGSFIGFSAGFAMLAKTQFPDVNILQLAFFGPFIGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LARSAGGVISDKFGGVRVTLINFIFMALFTALLFLTLPGSGAGSFSAFYLVFMGLFLTAG
HHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCC
LGSGSTFQMIAVIFRQITLYNVKLRGGSDEQAQREAVTDTAAALGFISAIGAVGGFFIPK
CCCCCHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AFGTSLALTGSPVGAMKIFLLFYIACVLLTWLVYGRLKPKQQ
HHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: nitrite [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: nitrite [Cytoplasm] + Proton [Periplasm] = Proton [Cytoplasm] + nitrite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677609; 7926834 [H]