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The map label for this gene is smvA [H]

Identifier: 29141930

GI number: 29141930

Start: 1533195

End: 1534682

Strand: Reverse

Name: smvA [H]

Synonym: t1485

Alternate gene names: 29141930

Gene position: 1534682-1533195 (Counterclockwise)

Preceding gene: 29141936

Following gene: 29141925

Centisome position: 32.03

GC content: 57.93

Gene sequence:

>1488_bases
ATGTTTCGTCAGTGGTTAACGTTAGTCATTATTGTGCTGGTCTATATTCCTGTCGCCATTGATGCCACGGTCTTGCATGT
CGCCGCGCCGACACTGAGTATGACACTGGGGGCCAGCGGCAACGAGCTGCTGTGGATCATTGATATTTATTCTTTGGTCA
TGGCTGGCATGGTGTTGCCGATGGGCGCGCTTGGCGATCGTATCGGTTTTAAACGCCTGCTGATGCTGGGCGGGACGCTC
TTTGGCCTGGCATCACTGGCGGCGGCGTTTTCGCATACCGCCAGTTGGCTTATCGCCACCAGGGTATTACTGGCTATTGG
CGCGGCGATGATTGTATCGGCGACGCTGGCCGGGATACGCGCCACCTTTTGTGAAGAGAAGCATCGCAACATGGCGCTGG
GCGTCTGGGCTGCGGTAGGTTCGGGCGGAGCGGCGTTTGGGCCGCTCATCGGCGGCATATTATTAGAGCATTTTTACTGG
GGATCGGTTTTCCTGATCAACGTGCCGATTGTGCTGGTCGTCATGGGCTTAACCGCCCGCTATGTTCCTCGTCAGGCGGG
CCGTCGCGATCAACCGCTCAATCTTGGCCATGCGGTGATGCTGATTATTGCCATTTTGCTGTTGGTCTATAGCGCTAAAA
CCGCGCTGAAAGGGAATTTGTCGCTGTGGGTCATCTCGCTTACCCTGCTTACCGGCGCGTTGCTACTGGGACTCTTTATC
CGCACACAGCTTGCGACATCGCGTCCGATGATTGATATGCGACTATTTACCCATCGCATTATCCTGAGCGGCGTCGTGAT
GGCGATGACCGCGATGATCACTCTGGTGGGTTTTGAGCTGCTGATGGCGCAAGAGCTACAGTTTGTTCACGGGCTATCGC
CTTATGAGGCCGGGGTATTTATGCTGCCGGTGATGGTCGCCAGTGGATTCAGCGGGCCGATTGCGGGCGCGCTGGTCTCG
CGTCTGGGGTTACGGCTGGTCGCGACGGGCGGCATGGCGTTAAGCGCGCTGAGTTTTTATGGTCTGGCGATGACGGATTT
CAGCACCCAACAATGGCAGGCCTGGGGGCTGATGGCGCTGCTGGGATTTAGCACCGCCAGCGCATTGCTGGCTTCCACGT
CGGCAATTATGGCCGCTGTGCCGGCAGAAAAAGCGGCGGCGGCCGGCGCGATAGAAACGATGGCTTATGAACTGGGCGCG
GGACTGGGCATCGCCATTTTCGGCCTGTTGTTAAGCCGTAGCTTCTCCGCGTCTATCCGTCTGCCTGCCGGGCTTGAGGC
GCAAGAGATTGCCAGAGCGTCATCTTCAATGGGAGAAGCCGTGCAGTTGGCGAATAGCCTGCCGCCCACGCAGGGGCAGG
CAATACTGGACGCCGCCAGACACGCCTTTATCTGGTCGCATAGCGTGGCGTTAAGCAGCGCCGGGAGTATGCTTCTTTTG
CTGGCGGTAGGGATGTGGTTCAGCCTGGCAAAAGCCCAACGCCGATAA

Upstream 100 bases:

>100_bases
CAATGCGCGCAGTGTAACAAAAAGCTGGACATTCGTTCAACTTACCGTAGGATCCTGAAATTGGACAGGTGTCCAGGTTT
AAGTTTTTGAAGGGAGAGTT

Downstream 100 bases:

>100_bases
CATAACGCCTGATGAGATAAGCGCTTAATGCGCTAGCTGGCAGGGGGAGTCTATTTCCTCAACCTCTTTACCGCTGAGGC
GTGCGTATTGCCCCGTCAGT

Product: methyl viologen resistance protein SmvA

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MFRQWLTLVIIVLVYIPVAIDATVLHVAAPTLSMTLGASGNELLWIIDIYSLVMAGMVLPMGALGDRIGFKRLLMLGGTL
FGLASLAAAFSHTASWLIATRVLLAIGAAMIVSATLAGIRATFCEEKHRNMALGVWAAVGSGGAAFGPLIGGILLEHFYW
GSVFLINVPIVLVVMGLTARYVPRQAGRRDQPLNLGHAVMLIIAILLLVYSAKTALKGNLSLWVISLTLLTGALLLGLFI
RTQLATSRPMIDMRLFTHRIILSGVVMAMTAMITLVGFELLMAQELQFVHGLSPYEAGVFMLPVMVASGFSGPIAGALVS
RLGLRLVATGGMALSALSFYGLAMTDFSTQQWQAWGLMALLGFSTASALLASTSAIMAAVPAEKAAAAGAIETMAYELGA
GLGIAIFGLLLSRSFSASIRLPAGLEAQEIARASSSMGEAVQLANSLPPTQGQAILDAARHAFIWSHSVALSSAGSMLLL
LAVGMWFSLAKAQRR

Sequences:

>Translated_495_residues
MFRQWLTLVIIVLVYIPVAIDATVLHVAAPTLSMTLGASGNELLWIIDIYSLVMAGMVLPMGALGDRIGFKRLLMLGGTL
FGLASLAAAFSHTASWLIATRVLLAIGAAMIVSATLAGIRATFCEEKHRNMALGVWAAVGSGGAAFGPLIGGILLEHFYW
GSVFLINVPIVLVVMGLTARYVPRQAGRRDQPLNLGHAVMLIIAILLLVYSAKTALKGNLSLWVISLTLLTGALLLGLFI
RTQLATSRPMIDMRLFTHRIILSGVVMAMTAMITLVGFELLMAQELQFVHGLSPYEAGVFMLPVMVASGFSGPIAGALVS
RLGLRLVATGGMALSALSFYGLAMTDFSTQQWQAWGLMALLGFSTASALLASTSAIMAAVPAEKAAAAGAIETMAYELGA
GLGIAIFGLLLSRSFSASIRLPAGLEAQEIARASSSMGEAVQLANSLPPTQGQAILDAARHAFIWSHSVALSSAGSMLLL
LAVGMWFSLAKAQRR
>Mature_495_residues
MFRQWLTLVIIVLVYIPVAIDATVLHVAAPTLSMTLGASGNELLWIIDIYSLVMAGMVLPMGALGDRIGFKRLLMLGGTL
FGLASLAAAFSHTASWLIATRVLLAIGAAMIVSATLAGIRATFCEEKHRNMALGVWAAVGSGGAAFGPLIGGILLEHFYW
GSVFLINVPIVLVVMGLTARYVPRQAGRRDQPLNLGHAVMLIIAILLLVYSAKTALKGNLSLWVISLTLLTGALLLGLFI
RTQLATSRPMIDMRLFTHRIILSGVVMAMTAMITLVGFELLMAQELQFVHGLSPYEAGVFMLPVMVASGFSGPIAGALVS
RLGLRLVATGGMALSALSFYGLAMTDFSTQQWQAWGLMALLGFSTASALLASTSAIMAAVPAEKAAAAGAIETMAYELGA
GLGIAIFGLLLSRSFSASIRLPAGLEAQEIARASSSMGEAVQLANSLPPTQGQAILDAARHAFIWSHSVALSSAGSMLLL
LAVGMWFSLAKAQRR

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=395, Percent_Identity=26.5822784810127, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1788710, Length=391, Percent_Identity=27.3657289002558, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI87081983, Length=399, Percent_Identity=26.0651629072682, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1790195, Length=399, Percent_Identity=27.8195488721804, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788392, Length=409, Percent_Identity=24.9388753056235, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=518, Percent_Identity=22.972972972973, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52098; Mature: 52098

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRQWLTLVIIVLVYIPVAIDATVLHVAAPTLSMTLGASGNELLWIIDIYSLVMAGMVLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCEEEHHHHHHHHHHHHHHC
MGALGDRIGFKRLLMLGGTLFGLASLAAAFSHTASWLIATRVLLAIGAAMIVSATLAGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATFCEEKHRNMALGVWAAVGSGGAAFGPLIGGILLEHFYWGSVFLINVPIVLVVMGLTAR
HHHHHHHHCCEEEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YVPRQAGRRDQPLNLGHAVMLIIAILLLVYSAKTALKGNLSLWVISLTLLTGALLLGLFI
HCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RTQLATSRPMIDMRLFTHRIILSGVVMAMTAMITLVGFELLMAQELQFVHGLSPYEAGVF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCHHH
MLPVMVASGFSGPIAGALVSRLGLRLVATGGMALSALSFYGLAMTDFSTQQWQAWGLMAL
HHHHHHHCCCCCHHHHHHHHHHCHHEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LGFSTASALLASTSAIMAAVPAEKAAAAGAIETMAYELGAGLGIAIFGLLLSRSFSASIR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEE
LPAGLEAQEIARASSSMGEAVQLANSLPPTQGQAILDAARHAFIWSHSVALSSAGSMLLL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LAVGMWFSLAKAQRR
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MFRQWLTLVIIVLVYIPVAIDATVLHVAAPTLSMTLGASGNELLWIIDIYSLVMAGMVLP
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCEEEHHHHHHHHHHHHHHC
MGALGDRIGFKRLLMLGGTLFGLASLAAAFSHTASWLIATRVLLAIGAAMIVSATLAGIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ATFCEEKHRNMALGVWAAVGSGGAAFGPLIGGILLEHFYWGSVFLINVPIVLVVMGLTAR
HHHHHHHHCCEEEEHHHHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YVPRQAGRRDQPLNLGHAVMLIIAILLLVYSAKTALKGNLSLWVISLTLLTGALLLGLFI
HCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RTQLATSRPMIDMRLFTHRIILSGVVMAMTAMITLVGFELLMAQELQFVHGLSPYEAGVF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCHHH
MLPVMVASGFSGPIAGALVSRLGLRLVATGGMALSALSFYGLAMTDFSTQQWQAWGLMAL
HHHHHHHCCCCCHHHHHHHHHHCHHEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LGFSTASALLASTSAIMAAVPAEKAAAAGAIETMAYELGAGLGIAIFGLLLSRSFSASIR
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEE
LPAGLEAQEIARASSSMGEAVQLANSLPPTQGQAILDAARHAFIWSHSVALSSAGSMLLL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
LAVGMWFSLAKAQRR
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7926834; 11677609 [H]