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The map label for this gene is treZ [H]

Identifier: 29141920

GI number: 29141920

Start: 1521254

End: 1523038

Strand: Reverse

Name: treZ [H]

Synonym: t1473

Alternate gene names: 29141920

Gene position: 1523038-1521254 (Counterclockwise)

Preceding gene: 29141921

Following gene: 29141919

Centisome position: 31.78

GC content: 54.17

Gene sequence:

>1785_bases
ATGAGTTCAAAAATTTTTTGCAAAAGCTGGGGGGCTGAATATATCGCCGCTGATGTCGTCCGCTTTCGTCTTTGGGCCAC
CGGTCAGCAAAAGGTTATGCTCAGGCTTGCGGGTAAAGACCAGGAAATGCAGGCGAGCGGTGACGGCTGGTTTACGCTGG
ATGTATCCGGGGTGACGCCAGGTACGGAGTATAACTTTGTACTCAGCGATGGCATGGTGCTCCCCGATCCGGCTTCCCGC
GCCCAAAAAACTGACGTCAATGGTCCGTCATATGTGATTGATCCAGGCAGCTACGCGTGGCGCAACACCGGGTGGAAAGG
CAGCCGTTGGGAGCAGGCCGTGGTGTACGAGATGCATACAGGCACGTTCACTCCGGAAGGCACCTTCCACACCGCAATAG
CGAAGCTGCCTTATCTCGCTGAACTCGGCGTTACCGTTATTGAAGTGATGCCCGTTGCGCAATTTGGCGGCGAGCGCGGC
TGGGGCTATGACGGCGTACTGCTTTACGCGCCGCATTCTGCCTATGGGACGCCGGATGATTTCAAAGCGTTTATTGACAC
CGCGCATGGGTATGGTCTTTCCGTCATCCTGGATATTGTGCTGAACCATTTCGGCCCGGAGGGAAATTATTTACCGCTAT
TGGCGCCGGCGTTTTTCCACAAAGAGCGCATGACGCCGTGGGGAAATGGTATCGCCTATGATGTCGACGCCGTGCGGCGC
TATATCATCGAGGCGCCGTTATACTGGCTGACAGAATACCATCTCGACGGCTTACGCTTTGACGCTATCGATCAGATTGA
GGACAGTAGCGCCAGGCATGTGCTGGTTGAAATCGCACAACGTATTCGGGAAGACATTACCGACAGACCCATTCATCTGA
CTACCGAAGATAGCCGCAATATTATTTCTCTGCATCCCCGTGATCAGGATGGCAATGCGCCGCTGTTTACCGCCGAATGG
AATGACGATTTTCATAATGCCGTCCACGTTTTTGCGACCGGAGAGACCCAGGCCTACTACAACGATTTTGCTGATGCCCC
GGAAAAACACCTCGCAAGAGCGCTGGCCGAAGGATTCGCTTATCAGGGGGAAATCTCACCGCAAACCGGCGAACCTCGCG
GCGTAAAAAGTACCGGACAACCCCCGGTCGCCTTTGTGGATTTTATTCAGAACCACGATCAGGTCGGTAACCGCGCCCAG
GGCGACAGACTGATAACCCTGGCGGGTGCTGAACGAACAAAAGTATTGCTCGCCACGTTGCTGCTTTCACCGCATATTCC
TCTGCTTTTTATGGGCGAAGAGTATGGCGAAAGCCGTCCTTTTCTTTTTTTTACCGATTTCCATGGGGATTTAGCCCGCG
CCGTTCGTGAAGGTCGCGCAAAAGAGTTTGCCGATCATGCAGGGGAAAATGTTCCGGACCCGAATGCGCCAGAGACCTTT
CAACGCTCAAAACTTAACTGGAAGCAACAGCACAGTGAAGAGGGTAAAGCGTGGCTGGCATTTACCCGCGAACTACTGCT
TTTGCGCCAGAAGCATATCGTGCCGCTGTTGTCCGCTGCCCGTGAGAGCTCAGGAACGGTATTGCAAACCGCGCCCGGGT
TTATTGCCGTTAGCTGGCGTTTTCCGGGAGGAACACTGTCACTGGCGCTGAATATTAGCGCCACGACGGTATTGCTGCCC
GATTTACCGGGTAAGACTCTCTTCGCCTGGCCGAATGAATCCACCGGGTCGCTTTCCCAACATTCTCTTATTGTCCGCTT
AGCCCAGGGAGAGTCTGCATCATGA

Upstream 100 bases:

>100_bases
AAGAATGCCTGTTAAGTGACAGTGTCACGTAAAAATTAGCCCCATAGTCTATGCTTGTTTTTCGGATGCTGTAGAGGTTA
TGAGTTAAACGGAGCGACAA

Downstream 100 bases:

>100_bases
TCCCAACTGCAACGTATCGTCTTCAGTTTCGCAATAGCATGACCTTCGATCGCGCTGCGGCGCTGGTGCCTTACCTTAAA
AATCTGGGTATCAGCCATCT

Product: hydrolase

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 594; Mature: 593

Protein sequence:

>594_residues
MSSKIFCKSWGAEYIAADVVRFRLWATGQQKVMLRLAGKDQEMQASGDGWFTLDVSGVTPGTEYNFVLSDGMVLPDPASR
AQKTDVNGPSYVIDPGSYAWRNTGWKGSRWEQAVVYEMHTGTFTPEGTFHTAIAKLPYLAELGVTVIEVMPVAQFGGERG
WGYDGVLLYAPHSAYGTPDDFKAFIDTAHGYGLSVILDIVLNHFGPEGNYLPLLAPAFFHKERMTPWGNGIAYDVDAVRR
YIIEAPLYWLTEYHLDGLRFDAIDQIEDSSARHVLVEIAQRIREDITDRPIHLTTEDSRNIISLHPRDQDGNAPLFTAEW
NDDFHNAVHVFATGETQAYYNDFADAPEKHLARALAEGFAYQGEISPQTGEPRGVKSTGQPPVAFVDFIQNHDQVGNRAQ
GDRLITLAGAERTKVLLATLLLSPHIPLLFMGEEYGESRPFLFFTDFHGDLARAVREGRAKEFADHAGENVPDPNAPETF
QRSKLNWKQQHSEEGKAWLAFTRELLLLRQKHIVPLLSAARESSGTVLQTAPGFIAVSWRFPGGTLSLALNISATTVLLP
DLPGKTLFAWPNESTGSLSQHSLIVRLAQGESAS

Sequences:

>Translated_594_residues
MSSKIFCKSWGAEYIAADVVRFRLWATGQQKVMLRLAGKDQEMQASGDGWFTLDVSGVTPGTEYNFVLSDGMVLPDPASR
AQKTDVNGPSYVIDPGSYAWRNTGWKGSRWEQAVVYEMHTGTFTPEGTFHTAIAKLPYLAELGVTVIEVMPVAQFGGERG
WGYDGVLLYAPHSAYGTPDDFKAFIDTAHGYGLSVILDIVLNHFGPEGNYLPLLAPAFFHKERMTPWGNGIAYDVDAVRR
YIIEAPLYWLTEYHLDGLRFDAIDQIEDSSARHVLVEIAQRIREDITDRPIHLTTEDSRNIISLHPRDQDGNAPLFTAEW
NDDFHNAVHVFATGETQAYYNDFADAPEKHLARALAEGFAYQGEISPQTGEPRGVKSTGQPPVAFVDFIQNHDQVGNRAQ
GDRLITLAGAERTKVLLATLLLSPHIPLLFMGEEYGESRPFLFFTDFHGDLARAVREGRAKEFADHAGENVPDPNAPETF
QRSKLNWKQQHSEEGKAWLAFTRELLLLRQKHIVPLLSAARESSGTVLQTAPGFIAVSWRFPGGTLSLALNISATTVLLP
DLPGKTLFAWPNESTGSLSQHSLIVRLAQGESAS
>Mature_593_residues
SSKIFCKSWGAEYIAADVVRFRLWATGQQKVMLRLAGKDQEMQASGDGWFTLDVSGVTPGTEYNFVLSDGMVLPDPASRA
QKTDVNGPSYVIDPGSYAWRNTGWKGSRWEQAVVYEMHTGTFTPEGTFHTAIAKLPYLAELGVTVIEVMPVAQFGGERGW
GYDGVLLYAPHSAYGTPDDFKAFIDTAHGYGLSVILDIVLNHFGPEGNYLPLLAPAFFHKERMTPWGNGIAYDVDAVRRY
IIEAPLYWLTEYHLDGLRFDAIDQIEDSSARHVLVEIAQRIREDITDRPIHLTTEDSRNIISLHPRDQDGNAPLFTAEWN
DDFHNAVHVFATGETQAYYNDFADAPEKHLARALAEGFAYQGEISPQTGEPRGVKSTGQPPVAFVDFIQNHDQVGNRAQG
DRLITLAGAERTKVLLATLLLSPHIPLLFMGEEYGESRPFLFFTDFHGDLARAVREGRAKEFADHAGENVPDPNAPETFQ
RSKLNWKQQHSEEGKAWLAFTRELLLLRQKHIVPLLSAARESSGTVLQTAPGFIAVSWRFPGGTLSLALNISATTVLLPD
LPGKTLFAWPNESTGSLSQHSLIVRLAQGESAS

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=164, Percent_Identity=29.2682926829268, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI2367229, Length=435, Percent_Identity=26.6666666666667, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1789839, Length=163, Percent_Identity=31.2883435582822, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320826, Length=166, Percent_Identity=27.710843373494, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28573410, Length=207, Percent_Identity=28.9855072463768, Blast_Score=74, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 65817; Mature: 65685

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKIFCKSWGAEYIAADVVRFRLWATGQQKVMLRLAGKDQEMQASGDGWFTLDVSGVTP
CCCCEEEHHCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCEECCCCCEEEEEECCCCC
GTEYNFVLSDGMVLPDPASRAQKTDVNGPSYVIDPGSYAWRNTGWKGSRWEQAVVYEMHT
CCCEEEEEECCEECCCCHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEEEC
GTFTPEGTFHTAIAKLPYLAELGVTVIEVMPVAQFGGERGWGYDGVLLYAPHSAYGTPDD
CCCCCCCCHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHH
FKAFIDTAHGYGLSVILDIVLNHFGPEGNYLPLLAPAFFHKERMTPWGNGIAYDVDAVRR
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCCCCEEEEHHHHHH
YIIEAPLYWLTEYHLDGLRFDAIDQIEDSSARHVLVEIAQRIREDITDRPIHLTTEDSRN
HHHHCCHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
IISLHPRDQDGNAPLFTAEWNDDFHNAVHVFATGETQAYYNDFADAPEKHLARALAEGFA
EEEECCCCCCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHCCC
YQGEISPQTGEPRGVKSTGQPPVAFVDFIQNHDQVGNRAQGDRLITLAGAERTKVLLATL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHH
LLSPHIPLLFMGEEYGESRPFLFFTDFHGDLARAVREGRAKEFADHAGENVPDPNAPETF
HHCCCCCEEEECHHHCCCCCEEEEECCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHH
QRSKLNWKQQHSEEGKAWLAFTRELLLLRQKHIVPLLSAARESSGTVLQTAPGFIAVSWR
HHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEE
FPGGTLSLALNISATTVLLPDLPGKTLFAWPNESTGSLSQHSLIVRLAQGESAS
CCCCEEEEEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure 
SSKIFCKSWGAEYIAADVVRFRLWATGQQKVMLRLAGKDQEMQASGDGWFTLDVSGVTP
CCCEEEHHCCCHHHHHHHHHHHEECCCCCEEEEEECCCCCCEECCCCCEEEEEECCCCC
GTEYNFVLSDGMVLPDPASRAQKTDVNGPSYVIDPGSYAWRNTGWKGSRWEQAVVYEMHT
CCCEEEEEECCEECCCCHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEEEEEEC
GTFTPEGTFHTAIAKLPYLAELGVTVIEVMPVAQFGGERGWGYDGVLLYAPHSAYGTPDD
CCCCCCCCHHHHHHHCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHH
FKAFIDTAHGYGLSVILDIVLNHFGPEGNYLPLLAPAFFHKERMTPWGNGIAYDVDAVRR
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHCCCCCCCCEEEEHHHHHH
YIIEAPLYWLTEYHLDGLRFDAIDQIEDSSARHVLVEIAQRIREDITDRPIHLTTEDSRN
HHHHCCHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCC
IISLHPRDQDGNAPLFTAEWNDDFHNAVHVFATGETQAYYNDFADAPEKHLARALAEGFA
EEEECCCCCCCCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHCCC
YQGEISPQTGEPRGVKSTGQPPVAFVDFIQNHDQVGNRAQGDRLITLAGAERTKVLLATL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHHH
LLSPHIPLLFMGEEYGESRPFLFFTDFHGDLARAVREGRAKEFADHAGENVPDPNAPETF
HHCCCCCEEEECHHHCCCCCEEEEECCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCHHH
QRSKLNWKQQHSEEGKAWLAFTRELLLLRQKHIVPLLSAARESSGTVLQTAPGFIAVSWR
HHHCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEEE
FPGGTLSLALNISATTVLLPDLPGKTLFAWPNESTGSLSQHSLIVRLAQGESAS
CCCCEEEEEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]