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The map label for this gene is glgX [H]

Identifier: 29141918

GI number: 29141918

Start: 1516861

End: 1518672

Strand: Reverse

Name: glgX [H]

Synonym: t1471

Alternate gene names: 29141918

Gene position: 1518672-1516861 (Counterclockwise)

Preceding gene: 29141919

Following gene: 29141916

Centisome position: 31.69

GC content: 51.43

Gene sequence:

>1812_bases
ATGACAACAAACAGGGCTTTTGAAATCAGATCAGGATACAGCCATACGCTTGGCGCGAATTATGACGGCGAAGGCGTGAA
TTTCGCTATTTTTTCCGCCCATGCGGAACGCGTCGAGCTTTGTCTTTACGATCCGAGTGGGGAGCATGAAATCGCTCGTC
TGGAGTTACCGGAATATACCGACGAAATATGGCACGGCTATGTCACGAAGTTGCAGCCCGGCGCGCTGTATGGGTACCGC
GTTTACGGTCCTTATGACCCGGAAAATGGTCATCGTTTTAACCCCAATAAGCTGCTTATTGACCCTTATGCCCGTGAGCT
GGTGGGCGATATTCAGTGGAACGATGCGCATTTCGCGTATCAATTACTTCATGACGATAAAGATTTGACCTTTGATGACC
AGGATAGCGCGCCATTTACGCCTAAATGTCGGGTGATTGATCCGGCTGAGGCTAACTGGGAGGATAGACAACGGCCTTCC
ATACCGTGGTCGAACGCCATTATTTATGAAACGCATGTAAAAGGCTTTACCCAGCTTAATTCGGCGATCCCTGAGCCTCT
ACGCGGTACGTTTGAGGGCATGGGGCATAAAGCCAGCGTGGATTACATTAAAAGTCTGGGGATTACCTCCGTCGAGCTTT
TACCCGTTCACTGGTTCCCGGACGATCAGCATCTGCTCGATAAAGGACTGAAAAATTTCTGGGGATATAACTCGCTGGGC
TTTTTTGCGCCGGCGACGCGCTACTATGGTCCAAAAGGTATTCAGGGATTCCGCGATATGGTGCGCGCTTTCCATGATGC
CGGCGTCGAAGTGATACTGGATGTGGTCTATAACCATACGGCAGAAGGGAATGAACTTGGCCCCACGCTGTCATTTAAAG
GTATCGATAACTTCTCTTACTATCGCACCATGCCCGATCAGCATCGCTATTATATTAACGATACAGGGACAGGTAATACC
GTCAATACCTCTCATCCACGGGTACTGCAAATGGTGATGGATTCGCTTCGCTATTGGGCGGAGTCAATGCATGTCGACGG
CTTTCGTTTTGATTTGGGCACCATTCTTGGTCGTGAGCCGGAAGGCTTTGATCAGAGAGGCGGCTTTTTTGATGCGGTAA
CGCAAGACCCGGTGCTGGCAAAACTGAAACTGATTGGCGAACCCTGGGATATCGGACCGGGCGGCTATCAGGTTGGCGGT
TTTCCACCAGGGTGGGGCGAATGGAATGATAAATATCGCGATACGGTTCGCGAATACTGGAAAGGTGACAACGTCACAAA
TGATTTCGCCGCACGCCTGCTGGGTTCCGGCGATCTCTACGATTTGCGCGGCCGTCGCTCCTGGTCAAGCGTTAACTTTA
TTACTGCCCATGACGGCTTCACGCTGAACGATCTGGTGTCTTATAACGACAAACATAATGAAGCCAATGGCGAAGATAAT
AATGACGGGCATAATGATAACCGCTCCTGTAACTATGGCGCAGAAGGGCCAACCGACGACGAGGGCATCAATGCGATACG
CGAGCGACAGAAACGTAATTTCCTGACCACCTTACTTTTCTCACACGGCACGCCGATGTTATTAGCGGGCGATGAGTTTG
GCCGCAGTCAGATGGGCAATAACAATGGCTACTGTCAGGACAGTGAAATATCGTGGGTCCACTGGGATAATTTGCCGGAA
ACCGCCAATGCGCTACGGGAGTTTACCCGTCATCTGATTCAACTGCGCGCCACGCAACCGTTGCTGCGCCGGGAGAGCTG
GCGTGACGGTCTGGAGATCCGCTGGTTTAACGCCGGCGGAGGGGCGCAGTAA

Upstream 100 bases:

>100_bases
TGGCCTCACATCAGGGCGGCGTACTGGTTTTAATGAGCGACTGAGTTTATGTGTTGATGTGCCGTGACAGGACAACGTTT
ACCGTGGGTGGAGAAAGAGA

Downstream 100 bases:

>100_bases
TCCGAACAATGGGATGAGGGATCGACGATAGGCGTATGTATTAGTCGACCCGATCTTCAGCCGGAAGCGGGGATCTGGCA
TGACGCCTTACTGCTTTTCA

Product: glycogen debranching protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 603; Mature: 602

Protein sequence:

>603_residues
MTTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVELCLYDPSGEHEIARLELPEYTDEIWHGYVTKLQPGALYGYR
VYGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHDDKDLTFDDQDSAPFTPKCRVIDPAEANWEDRQRPS
IPWSNAIIYETHVKGFTQLNSAIPEPLRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLG
FFAPATRYYGPKGIQGFRDMVRAFHDAGVEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMPDQHRYYINDTGTGNT
VNTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGG
FPPGWGEWNDKYRDTVREYWKGDNVTNDFAARLLGSGDLYDLRGRRSWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDN
NDGHNDNRSCNYGAEGPTDDEGINAIRERQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYCQDSEISWVHWDNLPE
TANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQ

Sequences:

>Translated_603_residues
MTTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVELCLYDPSGEHEIARLELPEYTDEIWHGYVTKLQPGALYGYR
VYGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHDDKDLTFDDQDSAPFTPKCRVIDPAEANWEDRQRPS
IPWSNAIIYETHVKGFTQLNSAIPEPLRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLG
FFAPATRYYGPKGIQGFRDMVRAFHDAGVEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMPDQHRYYINDTGTGNT
VNTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGG
FPPGWGEWNDKYRDTVREYWKGDNVTNDFAARLLGSGDLYDLRGRRSWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDN
NDGHNDNRSCNYGAEGPTDDEGINAIRERQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYCQDSEISWVHWDNLPE
TANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQ
>Mature_602_residues
TTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVELCLYDPSGEHEIARLELPEYTDEIWHGYVTKLQPGALYGYRV
YGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAYQLLHDDKDLTFDDQDSAPFTPKCRVIDPAEANWEDRQRPSI
PWSNAIIYETHVKGFTQLNSAIPEPLRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLGF
FAPATRYYGPKGIQGFRDMVRAFHDAGVEVILDVVYNHTAEGNELGPTLSFKGIDNFSYYRTMPDQHRYYINDTGTGNTV
NTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREPEGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGF
PPGWGEWNDKYRDTVREYWKGDNVTNDFAARLLGSGDLYDLRGRRSWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDNN
DGHNDNRSCNYGAEGPTDDEGINAIRERQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGNNNGYCQDSEISWVHWDNLPET
ANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGGGAQ

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG1523

COG function: function code G; Type II secretory pathway, pullulanase PulA and related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI2367229, Length=596, Percent_Identity=48.489932885906, Blast_Score=544, Evalue=1e-156,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR011837
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 68589; Mature: 68458

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVELCLYDPSGEHEIARLELPEYT
CCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCEEEEEECCHHH
DEIWHGYVTKLQPGALYGYRVYGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAY
HHHHHHHHHEECCCCEEEEEEECCCCCCCCCEECCCEEEECHHHHHHHHCCCCCCHHEEE
QLLHDDKDLTFDDQDSAPFTPKCRVIDPAEANWEDRQRPSIPWSNAIIYETHVKGFTQLN
EEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHH
SAIPEPLRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLG
HHCCHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCC
FFAPATRYYGPKGIQGFRDMVRAFHDAGVEVILDVVYNHTAEGNELGPTLSFKGIDNFSY
CCCCHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHH
YRTMPDQHRYYINDTGTGNTVNTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREP
HCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCC
EGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGFPPGWGEWNDKYRDTVREYW
CCCCCCCCEECCCCCCCHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHH
KGDNVTNDFAARLLGSGDLYDLRGRRSWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDN
CCCCCCHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCC
NDGHNDNRSCNYGAEGPTDDEGINAIRERQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCHHHCCC
NNGYCQDSEISWVHWDNLPETANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGG
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCC
GAQ
CCC
>Mature Secondary Structure 
TTNRAFEIRSGYSHTLGANYDGEGVNFAIFSAHAERVELCLYDPSGEHEIARLELPEYT
CCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCEEEEEECCHHH
DEIWHGYVTKLQPGALYGYRVYGPYDPENGHRFNPNKLLIDPYARELVGDIQWNDAHFAY
HHHHHHHHHEECCCCEEEEEEECCCCCCCCCEECCCEEEECHHHHHHHHCCCCCCHHEEE
QLLHDDKDLTFDDQDSAPFTPKCRVIDPAEANWEDRQRPSIPWSNAIIYETHVKGFTQLN
EEEECCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCEEEEEECHHHHHHHH
SAIPEPLRGTFEGMGHKASVDYIKSLGITSVELLPVHWFPDDQHLLDKGLKNFWGYNSLG
HHCCHHHHHHHHCCCCCCHHHHHHHCCCCEEEEEEEEECCCHHHHHHHHHHHHCCCCCCC
FFAPATRYYGPKGIQGFRDMVRAFHDAGVEVILDVVYNHTAEGNELGPTLSFKGIDNFSY
CCCCHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCEEEECCCCCCHH
YRTMPDQHRYYINDTGTGNTVNTSHPRVLQMVMDSLRYWAESMHVDGFRFDLGTILGREP
HCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCCC
EGFDQRGGFFDAVTQDPVLAKLKLIGEPWDIGPGGYQVGGFPPGWGEWNDKYRDTVREYW
CCCCCCCCEECCCCCCCHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHH
KGDNVTNDFAARLLGSGDLYDLRGRRSWSSVNFITAHDGFTLNDLVSYNDKHNEANGEDN
CCCCCCHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCC
NDGHNDNRSCNYGAEGPTDDEGINAIRERQKRNFLTTLLFSHGTPMLLAGDEFGRSQMGN
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHCHHHCCC
NNGYCQDSEISWVHWDNLPETANALREFTRHLIQLRATQPLLRRESWRDGLEIRWFNAGG
CCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEECCC
GAQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Glycosidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]