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The map label for this gene is patB [H]

Identifier: 29141917

GI number: 29141917

Start: 1515303

End: 1516484

Strand: Direct

Name: patB [H]

Synonym: t1470

Alternate gene names: 29141917

Gene position: 1515303-1516484 (Clockwise)

Preceding gene: 29141915

Following gene: 29141923

Centisome position: 31.62

GC content: 51.78

Gene sequence:

>1182_bases
GTGTGCAACAATACTGGTTCGGTAAAGTGGGATTTTATTGAGCGTCACTTTGGCGACGGCGCTGGAAAACTGCTGCCGAT
GTGGGTTTCTGATTTCGATTTTGCCTGCCCGCCAGAGGTTCAGGCGGCATTGCATCAGCGAATCGAGCACGGCGTATTTG
GCTATAGCGAACGTGATGAGGCCTATTTTAACGCCCTGCTTCACTGGTTTTCGTCACGCCACCAGCTCACGTTAAAACAG
GAATGGGTATGCAGCGTGGAAGGCGTCGTACCAGGGTTGGCGCTCTTAGTGCAAATGTTGACGCATCCAGGCGACGGCGT
AGTGGTTCAGGGGCCATACTATGGCTCCTTCGCCAAAATAATCACGCTTAATGGTAGAAAACTGCTGGAGAATCCCCTTA
GCGAGTCGCCGGATGAAGGCTACCAGATGGATTTTTGCCAACTGGAAAGGCTGTTTCGCCATGAGCGCCCTCCGCTAATG
ATTTTGTGTAATCCACATAATCCAACCGGGCGTTGCTGGTCGGCCAACGAGCTGGAGCAACTGCTTTTATTATGTGAAGC
TTATGATGTCACTCTCATCTCGGATGAAATATGGGCCGATCTATTGCTGCCCTGCGAAACCTTTACCTCGGTACTGCATT
TAGGGGAGCGCTGGCATAAACGGGTCATCTCCGCCACTGCGGCCAGCAAAACGTTTGGCTTATCGTCGTTGCGAATTTCT
AATTTTCTCATTCCCGATCCTACGCTTCGCCAGCGTTTCCTGTCTCGTCTGGACGCTCATGGGCTGGATGTCTTTAACGC
CCTCTCCGTTCAGGCGGCAACTACCGCATGGAATGAAAGCAGACAGTGGCTGGATTCGCTACTGGATTATCTGGCGGAAA
ACCGCCGCTGGTTTGTTGAACAGGCGACAGAGCACCTTCCCTGGGCAAGAATTGTCCCGGCGCAGGGAACCTATTTATTA
TGGATGGATTGTAAAAAACTGGGACTGGATGACGAACAGCTAAAATGGGTAATGGCGGATGTCGCGGGTATTGCTCCTTC
AATGGGCAGCGGCTTTGGCCCGGCGGGCAGCGGATTTATCCGTTTAAATCTGGGATGTCCACGTACCTACCTTGAAATGG
CAATAGACGGCCTGAAGCGAATATCGGTGAAGACAATCAAAGGAATACCAACAGGCGGATGA

Upstream 100 bases:

>100_bases
TTGTTCCTCGGCGTCATCATGCAGGGACTGAGCATCAAACAGGGACTGGACGCTTTCATTGATGGATTTGATATCGCCAT
GTTCCCACAAGGCGCAGAAG

Downstream 100 bases:

>100_bases
ATTTTTCGGGCGGTAAACTGACAACGGTGTTATTTCTGCGGCATGATATCGCCTGAGCGCCAGTAAAAAACCGGCCATTA
AGGCCGGTTGGGTCCGGAGC

Product: aminotransferase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MCNNTGSVKWDFIERHFGDGAGKLLPMWVSDFDFACPPEVQAALHQRIEHGVFGYSERDEAYFNALLHWFSSRHQLTLKQ
EWVCSVEGVVPGLALLVQMLTHPGDGVVVQGPYYGSFAKIITLNGRKLLENPLSESPDEGYQMDFCQLERLFRHERPPLM
ILCNPHNPTGRCWSANELEQLLLLCEAYDVTLISDEIWADLLLPCETFTSVLHLGERWHKRVISATAASKTFGLSSLRIS
NFLIPDPTLRQRFLSRLDAHGLDVFNALSVQAATTAWNESRQWLDSLLDYLAENRRWFVEQATEHLPWARIVPAQGTYLL
WMDCKKLGLDDEQLKWVMADVAGIAPSMGSGFGPAGSGFIRLNLGCPRTYLEMAIDGLKRISVKTIKGIPTGG

Sequences:

>Translated_393_residues
MCNNTGSVKWDFIERHFGDGAGKLLPMWVSDFDFACPPEVQAALHQRIEHGVFGYSERDEAYFNALLHWFSSRHQLTLKQ
EWVCSVEGVVPGLALLVQMLTHPGDGVVVQGPYYGSFAKIITLNGRKLLENPLSESPDEGYQMDFCQLERLFRHERPPLM
ILCNPHNPTGRCWSANELEQLLLLCEAYDVTLISDEIWADLLLPCETFTSVLHLGERWHKRVISATAASKTFGLSSLRIS
NFLIPDPTLRQRFLSRLDAHGLDVFNALSVQAATTAWNESRQWLDSLLDYLAENRRWFVEQATEHLPWARIVPAQGTYLL
WMDCKKLGLDDEQLKWVMADVAGIAPSMGSGFGPAGSGFIRLNLGCPRTYLEMAIDGLKRISVKTIKGIPTGG
>Mature_393_residues
MCNNTGSVKWDFIERHFGDGAGKLLPMWVSDFDFACPPEVQAALHQRIEHGVFGYSERDEAYFNALLHWFSSRHQLTLKQ
EWVCSVEGVVPGLALLVQMLTHPGDGVVVQGPYYGSFAKIITLNGRKLLENPLSESPDEGYQMDFCQLERLFRHERPPLM
ILCNPHNPTGRCWSANELEQLLLLCEAYDVTLISDEIWADLLLPCETFTSVLHLGERWHKRVISATAASKTFGLSSLRIS
NFLIPDPTLRQRFLSRLDAHGLDVFNALSVQAATTAWNESRQWLDSLLDYLAENRRWFVEQATEHLPWARIVPAQGTYLL
WMDCKKLGLDDEQLKWVMADVAGIAPSMGSGFGPAGSGFIRLNLGCPRTYLEMAIDGLKRISVKTIKGIPTGG

Specific function: Catalyzes the transformation of cystathionine to homocysteine. Also exhibits cysteine desulfhydrase activity in vitro, producing sulfide from cysteine [H]

COG id: COG1168

COG function: function code E; Bifunctional PLP-dependent enzyme with beta-cystathionase and maltose regulon repressor activities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. MalY/PatB cystathionine beta-lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787909, Length=386, Percent_Identity=32.9015544041451, Blast_Score=211, Evalue=4e-56,
Organism=Escherichia coli, GI1788722, Length=357, Percent_Identity=25.4901960784314, Blast_Score=82, Evalue=9e-17,
Organism=Escherichia coli, GI1786816, Length=307, Percent_Identity=23.4527687296417, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322401, Length=184, Percent_Identity=26.6304347826087, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004839
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 44222; Mature: 44222

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCNNTGSVKWDFIERHFGDGAGKLLPMWVSDFDFACPPEVQAALHQRIEHGVFGYSERDE
CCCCCCCEEHHHHHHHCCCCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
AYFNALLHWFSSRHQLTLKQEWVCSVEGVVPGLALLVQMLTHPGDGVVVQGPYYGSFAKI
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEE
ITLNGRKLLENPLSESPDEGYQMDFCQLERLFRHERPPLMILCNPHNPTGRCWSANELEQ
EEECCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHH
LLLLCEAYDVTLISDEIWADLLLPCETFTSVLHLGERWHKRVISATAASKTFGLSSLRIS
HHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHC
NFLIPDPTLRQRFLSRLDAHGLDVFNALSVQAATTAWNESRQWLDSLLDYLAENRRWFVE
CEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHH
QATEHLPWARIVPAQGTYLLWMDCKKLGLDDEQLKWVMADVAGIAPSMGSGFGPAGSGFI
HHHHHCCCEEEECCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
RLNLGCPRTYLEMAIDGLKRISVKTIKGIPTGG
EEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MCNNTGSVKWDFIERHFGDGAGKLLPMWVSDFDFACPPEVQAALHQRIEHGVFGYSERDE
CCCCCCCEEHHHHHHHCCCCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHH
AYFNALLHWFSSRHQLTLKQEWVCSVEGVVPGLALLVQMLTHPGDGVVVQGPYYGSFAKI
HHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCEEEE
ITLNGRKLLENPLSESPDEGYQMDFCQLERLFRHERPPLMILCNPHNPTGRCWSANELEQ
EEECCHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHH
LLLLCEAYDVTLISDEIWADLLLPCETFTSVLHLGERWHKRVISATAASKTFGLSSLRIS
HHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHC
NFLIPDPTLRQRFLSRLDAHGLDVFNALSVQAATTAWNESRQWLDSLLDYLAENRRWFVE
CEECCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHH
QATEHLPWARIVPAQGTYLLWMDCKKLGLDDEQLKWVMADVAGIAPSMGSGFGPAGSGFI
HHHHHCCCEEEECCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEE
RLNLGCPRTYLEMAIDGLKRISVKTIKGIPTGG
EEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497199; 9274030; 9384377 [H]