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The map label for this gene is hyaA [H]

Identifier: 29141907

GI number: 29141907

Start: 1500712

End: 1501815

Strand: Reverse

Name: hyaA [H]

Synonym: t1458

Alternate gene names: 29141907

Gene position: 1501815-1500712 (Counterclockwise)

Preceding gene: 29141910

Following gene: 29141906

Centisome position: 31.34

GC content: 51.54

Gene sequence:

>1104_bases
ATGCAAACACAAGATACATTTTATCAAGTTATGCGTAGACACGGCGTGACAAGGCGTAGCTTCCTCAAGTTTTGCAGCCT
TACCGCAACGTCTTTAGGCCTGAGCAGTTCGATGATTCCTCAGATAGCTTATGCCCTGGAGAATAAACCGCGTACGCCAG
TGATATGGCTGCATGGTCTTGAATGTACCTGCTGTACCGAATCTTTCATTCGCTCTGCGCACCCGTTGGCAAAAGATGCC
ATTTTGTCGCTCATTTCCCTGGATTATGACGACACCATTATGGCCGCCGCAGGGCAACAAGCCGAGCAGGCGTTGGCGGA
TGTGATGCGTGAGTACAAAGGAAATTATATCGTTGCCGTGGAAGGTAACGCGCCGCTGAATGAAGATGGAATGTTTTGCA
TACTGGCTGGCGAGCCGTTCCTGGAAAAGTTAAAACGCGTCAGCGCGGATGCTAAAGCGATTATCGCCTGGGGGTCCTGC
GCATCATGGGGCTGCGTGCAGGCAGCCCGTCCCAACCCAACTAAAGCCACGCCGGTTCACAAATTAATTACCGATAAAAC
CATTATTAAAGTGCCAGGCTGTCCGCCTATACCTGAAGTGATGTCCGCCGTTATCACCTATATGCTGGCATTTGATCGCA
TCCCTCCCCTTGATCGTCTTGGACGACCCAAAATGTTTTATGGCCAGCGAATTCATGACAAATGTTACCGACGCGCGCAT
TTCGATGCCGGTCAGTTTGTGGAAGCGTGGGATGACGAAGGCGCCAGAAAAGGGTATTGCCTGTACAAGATGGGCTGTAA
AGGCCCGACTACCTATAACGCCTGTTCAACCGTTCGCTGGAATGATGGCGTGTCTTTTCCTATCCAGTCGGGTCATGGAT
GCCTCGGATGTTCGGAAGATGGATTCTGGGATTACGGTTCTTTTTACAGCCGGGCAACGGGCATTCCGCAAACCGGCATT
GAGGCTACCGCCGATAAAATCGGACTGGGCGTTGCCGGCGTCGCGGGCGCTGCTGCCATCGCTCACGCCACGGTTAGCGC
CATAAAACACGCCCGCAATAAAAACAACACGTCATCAGAAAACGCTACGGAAAAGAAAAAATAA

Upstream 100 bases:

>100_bases
TTACGAATTAGAAACATAATTGCTACTAAAACACGTTGAGAAAGACAAGAAATGCAACATATGATGCCCACACTAACTAT
AGTGGTTTGGGATAAACGTT

Downstream 100 bases:

>100_bases
TTATGGCATATCCTTATCAGACTCAGGGTTTTACGCTGGACAATAGCGGACGGCGAATTGTGGTCGATCCGGTTACTCGC
ATCGAAGAGCATATGCGCTG

Product: uptake hydrogenase small subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase small subunit [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQTQDTFYQVMRRHGVTRRSFLKFCSLTATSLGLSSSMIPQIAYALENKPRTPVIWLHGLECTCCTESFIRSAHPLAKDA
ILSLISLDYDDTIMAAAGQQAEQALADVMREYKGNYIVAVEGNAPLNEDGMFCILAGEPFLEKLKRVSADAKAIIAWGSC
ASWGCVQAARPNPTKATPVHKLITDKTIIKVPGCPPIPEVMSAVITYMLAFDRIPPLDRLGRPKMFYGQRIHDKCYRRAH
FDAGQFVEAWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNDGVSFPIQSGHGCLGCSEDGFWDYGSFYSRATGIPQTGI
EATADKIGLGVAGVAGAAAIAHATVSAIKHARNKNNTSSENATEKKK

Sequences:

>Translated_367_residues
MQTQDTFYQVMRRHGVTRRSFLKFCSLTATSLGLSSSMIPQIAYALENKPRTPVIWLHGLECTCCTESFIRSAHPLAKDA
ILSLISLDYDDTIMAAAGQQAEQALADVMREYKGNYIVAVEGNAPLNEDGMFCILAGEPFLEKLKRVSADAKAIIAWGSC
ASWGCVQAARPNPTKATPVHKLITDKTIIKVPGCPPIPEVMSAVITYMLAFDRIPPLDRLGRPKMFYGQRIHDKCYRRAH
FDAGQFVEAWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNDGVSFPIQSGHGCLGCSEDGFWDYGSFYSRATGIPQTGI
EATADKIGLGVAGVAGAAAIAHATVSAIKHARNKNNTSSENATEKKK
>Mature_367_residues
MQTQDTFYQVMRRHGVTRRSFLKFCSLTATSLGLSSSMIPQIAYALENKPRTPVIWLHGLECTCCTESFIRSAHPLAKDA
ILSLISLDYDDTIMAAAGQQAEQALADVMREYKGNYIVAVEGNAPLNEDGMFCILAGEPFLEKLKRVSADAKAIIAWGSC
ASWGCVQAARPNPTKATPVHKLITDKTIIKVPGCPPIPEVMSAVITYMLAFDRIPPLDRLGRPKMFYGQRIHDKCYRRAH
FDAGQFVEAWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNDGVSFPIQSGHGCLGCSEDGFWDYGSFYSRATGIPQTGI
EATADKIGLGVAGVAGAAAIAHATVSAIKHARNKNNTSSENATEKKK

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG1740

COG function: function code C; Ni,Fe-hydrogenase I small subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase small subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787206, Length=360, Percent_Identity=73.3333333333333, Blast_Score=558, Evalue=1e-160,
Organism=Escherichia coli, GI1789371, Length=310, Percent_Identity=41.6129032258065, Blast_Score=278, Evalue=3e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006137
- InterPro:   IPR001821
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01058 Oxidored_q6 [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 40037; Mature: 40037

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTQDTFYQVMRRHGVTRRSFLKFCSLTATSLGLSSSMIPQIAYALENKPRTPVIWLHGL
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECC
ECTCCTESFIRSAHPLAKDAILSLISLDYDDTIMAAAGQQAEQALADVMREYKGNYIVAV
CHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEEE
EGNAPLNEDGMFCILAGEPFLEKLKRVSADAKAIIAWGSCASWGCVQAARPNPTKATPVH
CCCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHH
KLITDKTIIKVPGCPPIPEVMSAVITYMLAFDRIPPLDRLGRPKMFYGQRIHDKCYRRAH
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHC
FDAGQFVEAWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNDGVSFPIQSGHGCLGCSED
CCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
GFWDYGSFYSRATGIPQTGIEATADKIGLGVAGVAGAAAIAHATVSAIKHARNKNNTSSE
CCCCCHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NATEKKK
CCCCCCC
>Mature Secondary Structure
MQTQDTFYQVMRRHGVTRRSFLKFCSLTATSLGLSSSMIPQIAYALENKPRTPVIWLHGL
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEECC
ECTCCTESFIRSAHPLAKDAILSLISLDYDDTIMAAAGQQAEQALADVMREYKGNYIVAV
CHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHCCCEEEEE
EGNAPLNEDGMFCILAGEPFLEKLKRVSADAKAIIAWGSCASWGCVQAARPNPTKATPVH
CCCCCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHH
KLITDKTIIKVPGCPPIPEVMSAVITYMLAFDRIPPLDRLGRPKMFYGQRIHDKCYRRAH
HHHCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHCCCCCHHHHHHHHHHHHHHHC
FDAGQFVEAWDDEGARKGYCLYKMGCKGPTTYNACSTVRWNDGVSFPIQSGHGCLGCSED
CCCCHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCC
GFWDYGSFYSRATGIPQTGIEATADKIGLGVAGVAGAAAIAHATVSAIKHARNKNNTSSE
CCCCCHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NATEKKK
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1383192; 12948488; 2493816 [H]