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The map label for this gene is hyaF [H]

Identifier: 29141902

GI number: 29141902

Start: 1495814

End: 1496875

Strand: Reverse

Name: hyaF [H]

Synonym: t1452

Alternate gene names: 29141902

Gene position: 1496875-1495814 (Counterclockwise)

Preceding gene: 29141903

Following gene: 29141901

Centisome position: 31.24

GC content: 50.0

Gene sequence:

>1062_bases
GTGAATCATTCCAGAACTATTCCAGTCGTTAATATTGCCGGACCAGGATCGCAGCCAGAAGAGGAAGACTTTAACTTTCT
TCCCATCCCCGCCGGCATTAATCTCCCGCTGACGCCGGTTTTACCGGAACAGGCGCTGCCCGCTGAGCTCCGTGTTGCCA
GACACATCCTGACTACGCTTATTCGCGATATGGATAACCCGGTGGCAACGCTCCCCTTTCCCCTGAGCTATAAGCTGAAT
GCCACTGAGCAACAGAATAGCGGTTTATTGGATCAACTGCTCGGCGAAGGTGAAATCTCCGCCCGGGTACTATTATCCGA
TGGAAAAGAACAGCGTATTCAGGAGACGGTTTTTACGGGCGTCTGGCGTGTACGTGAATATAACGCTGACCAGCAACGGG
TTGCCGATGAAATTATCATTGGCCCGATCCCGGAGAGCATCTGGCAGACGCATCCGCAGCCGCCGATTACACCAGAATTG
CCGCCACAACTGGCGGGATTGATGAATGGCGCCTTTATCGCGCATGAAATAGCCGAGCGCGTAAAACAGCCGGTAAAAGA
GCCGCATATCATTAATTTAACGCTATTGCCAGTAAACGATGCCGATCGCGAGTATCTGGATCATTTTTTAGGCGAAGGCT
GTAGCGCTATTTTTTCACGCGGATATGGTAAATGCCGGATTGTAAGCACGCATTTTCCCGGCGTATGGCGGGTCAATTAT
TTCAATGATATGAACACATTACTGCAAGATATGATTGAGATAGCGGACATTCCTGATATCGCCGTTGCAGGCATCGACGA
TATCGAAGATGCCTACACGGGGCTAAAAAATACGTTGGAATGGTTGAAAGAATACCCGGTCACAGAAAATGAGCCAGTGG
TGCGCATGGAGTGCAAAGTATGTTGGTGGGTTTACGACCCTGCGCTGGGCGATGATGTATGGCAAATTCCACCCGGCGTG
CCCTTCAACCAGTTACCTGATTACTGGTGCTGTCCGGTTTGCGAAACCAGTAAGTCCGGGTTTATGGTGATCGATGAAGG
TAATAATTCGTGCAAAGATTGA

Upstream 100 bases:

>100_bases
GCTGGCAAGAATACTCTCAGCGGGTGGCCGCATTGCTGACTACACCGGGCTATCGTCAGGATATTCCCGTTATTACGCAA
TAGAATGAAAGGTAACACAC

Downstream 100 bases:

>100_bases
CCGTCTATTCTCACCCGCTATGGATTACCTGGCAGGAGGCGCCGATCGGTCGTTTGTTACAGGGCGCAACGCCGGTATAC
GCAAAAACGCTTATTTCACG

Product: hydrogenase-1 operon protein HyaF2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MNHSRTIPVVNIAGPGSQPEEEDFNFLPIPAGINLPLTPVLPEQALPAELRVARHILTTLIRDMDNPVATLPFPLSYKLN
ATEQQNSGLLDQLLGEGEISARVLLSDGKEQRIQETVFTGVWRVREYNADQQRVADEIIIGPIPESIWQTHPQPPITPEL
PPQLAGLMNGAFIAHEIAERVKQPVKEPHIINLTLLPVNDADREYLDHFLGEGCSAIFSRGYGKCRIVSTHFPGVWRVNY
FNDMNTLLQDMIEIADIPDIAVAGIDDIEDAYTGLKNTLEWLKEYPVTENEPVVRMECKVCWWVYDPALGDDVWQIPPGV
PFNQLPDYWCCPVCETSKSGFMVIDEGNNSCKD

Sequences:

>Translated_353_residues
MNHSRTIPVVNIAGPGSQPEEEDFNFLPIPAGINLPLTPVLPEQALPAELRVARHILTTLIRDMDNPVATLPFPLSYKLN
ATEQQNSGLLDQLLGEGEISARVLLSDGKEQRIQETVFTGVWRVREYNADQQRVADEIIIGPIPESIWQTHPQPPITPEL
PPQLAGLMNGAFIAHEIAERVKQPVKEPHIINLTLLPVNDADREYLDHFLGEGCSAIFSRGYGKCRIVSTHFPGVWRVNY
FNDMNTLLQDMIEIADIPDIAVAGIDDIEDAYTGLKNTLEWLKEYPVTENEPVVRMECKVCWWVYDPALGDDVWQIPPGV
PFNQLPDYWCCPVCETSKSGFMVIDEGNNSCKD
>Mature_353_residues
MNHSRTIPVVNIAGPGSQPEEEDFNFLPIPAGINLPLTPVLPEQALPAELRVARHILTTLIRDMDNPVATLPFPLSYKLN
ATEQQNSGLLDQLLGEGEISARVLLSDGKEQRIQETVFTGVWRVREYNADQQRVADEIIIGPIPESIWQTHPQPPITPEL
PPQLAGLMNGAFIAHEIAERVKQPVKEPHIINLTLLPVNDADREYLDHFLGEGCSAIFSRGYGKCRIVSTHFPGVWRVNY
FNDMNTLLQDMIEIADIPDIAVAGIDDIEDAYTGLKNTLEWLKEYPVTENEPVVRMECKVCWWVYDPALGDDVWQIPPGV
PFNQLPDYWCCPVCETSKSGFMVIDEGNNSCKD

Specific function: Not Known. Could Enhance The Incorporation Of Nickel To The Hydrogenase. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupH/hyaF family [H]

Homologues:

Organism=Escherichia coli, GI1787211, Length=276, Percent_Identity=31.8840579710145, Blast_Score=144, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006894 [H]

Pfam domain/function: PF04809 HupH_C [H]

EC number: NA

Molecular weight: Translated: 39574; Mature: 39574

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS50903 RUBREDOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSRTIPVVNIAGPGSQPEEEDFNFLPIPAGINLPLTPVLPEQALPAELRVARHILTTL
CCCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IRDMDNPVATLPFPLSYKLNATEQQNSGLLDQLLGEGEISARVLLSDGKEQRIQETVFTG
HHHCCCCCEECCCCCEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
VWRVREYNADQQRVADEIIIGPIPESIWQTHPQPPITPELPPQLAGLMNGAFIAHEIAER
HHHHHHCCCHHHHHHHHHEECCCCHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHH
VKQPVKEPHIINLTLLPVNDADREYLDHFLGEGCSAIFSRGYGKCRIVSTHFPGVWRVNY
HHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCEEEEEE
FNDMNTLLQDMIEIADIPDIAVAGIDDIEDAYTGLKNTLEWLKEYPVTENEPVVRMECKV
HHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
CWWVYDPALGDDVWQIPPGVPFNQLPDYWCCPVCETSKSGFMVIDEGNNSCKD
EEEEECCCCCCCCEECCCCCCHHHCCCCEECCCCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MNHSRTIPVVNIAGPGSQPEEEDFNFLPIPAGINLPLTPVLPEQALPAELRVARHILTTL
CCCCCCCEEEEECCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
IRDMDNPVATLPFPLSYKLNATEQQNSGLLDQLLGEGEISARVLLSDGKEQRIQETVFTG
HHHCCCCCEECCCCCEEEECCCCCCCCCHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHH
VWRVREYNADQQRVADEIIIGPIPESIWQTHPQPPITPELPPQLAGLMNGAFIAHEIAER
HHHHHHCCCHHHHHHHHHEECCCCHHHHCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHH
VKQPVKEPHIINLTLLPVNDADREYLDHFLGEGCSAIFSRGYGKCRIVSTHFPGVWRVNY
HHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHHCCCCEEEEEEECCCCEEEEEE
FNDMNTLLQDMIEIADIPDIAVAGIDDIEDAYTGLKNTLEWLKEYPVTENEPVVRMECKV
HHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEE
CWWVYDPALGDDVWQIPPGVPFNQLPDYWCCPVCETSKSGFMVIDEGNNSCKD
EEEEECCCCCCCCEECCCCCCHHHCCCCEECCCCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1383192; 12948488 [H]