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The map label for this gene is rnfA [H]

Identifier: 29141786

GI number: 29141786

Start: 1388337

End: 1388918

Strand: Reverse

Name: rnfA [H]

Synonym: t1327

Alternate gene names: 29141786

Gene position: 1388918-1388337 (Counterclockwise)

Preceding gene: 29141787

Following gene: 29141785

Centisome position: 28.98

GC content: 50.17

Gene sequence:

>582_bases
ATGACTGATTACCTGCTGCTCTTTGTCGGAACTGTACTGGTCAATAACTTTGTACTGGTCAAATTCCTGGGCCTTTGTCC
CTTTATGGGCGTATCCAAAAAACTGGAAACCGCCATGGGGATGGGCCTCGCGACGACCTTTGTCATGACGCTGGCGTCGA
TATGCGCCTGGCTTATCGACACCTGGATACTTATTCCGCTGGATCTGATTTATCTGCGGACGCTGGCCTTTATCCTCGTC
ATTGCTGTGGTCGTGCAATTTACCGAAATGGTTGTGCGGAAAACCAGCCCGGCGTTATACCGCTTGTTAGGCATCTTCTT
ACCACTGATTACCACTAACTGTGCGGTTCTGGGCGTAGCGTTACTGAATATTAATCTCGGTCATCACTTTCTTCAGTCGG
CGCTGTACGGATTTTCCGCCGCCGTCGGTTTTTCATTAGTGATGGTTCTGTTTGCCGCTATCCGCGAACGCCTTGCCGTG
GCGGATGTGCCCGCCCCTTTTCGTGGTAACGCGATAGCATTGATTACCGCAGGATTAATGTCTTTGGCCTTTATGGGCTT
TAGTGGTTTGGTGAAGTTGTAA

Upstream 100 bases:

>100_bases
AAAACAACGTTTGTTAATCCGTAATAACCCTACAGTTAACCTGTTGTCGCCTGTGGTAGTTTGACAGATAATAGGCGGCT
TTTTTATTTCAGGCCGAAAC

Downstream 100 bases:

>100_bases
TGAATACTATCTGGATTGCCGTTGGCGCCCTGACCCTTCTGGGTCTGGTGTTCGGCGCCATTCTGGGTTATGCCTCCCGC
CGATTCGCGGTTGAAGATGA

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLATTFVMTLASICAWLIDTWILIPLDLIYLRTLAFILV
IAVVVQFTEMVVRKTSPALYRLLGIFLPLITTNCAVLGVALLNINLGHHFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL

Sequences:

>Translated_193_residues
MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLATTFVMTLASICAWLIDTWILIPLDLIYLRTLAFILV
IAVVVQFTEMVVRKTSPALYRLLGIFLPLITTNCAVLGVALLNINLGHHFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL
>Mature_192_residues
TDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLATTFVMTLASICAWLIDTWILIPLDLIYLRTLAFILVI
AVVVQFTEMVVRKTSPALYRLLGIFLPLITTNCAVLGVALLNINLGHHFLQSALYGFSAAVGFSLVMVLFAAIRERLAVA
DVPAPFRGNAIALITAGLMSLAFMGFSGLVKL

Specific function: May be part of a membrane complex involved in electron transport [H]

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family [H]

Homologues:

Organism=Escherichia coli, GI1787914, Length=193, Percent_Identity=98.4455958549223, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1787919, Length=156, Percent_Identity=35.8974358974359, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003667
- InterPro:   IPR011293 [H]

Pfam domain/function: PF02508 Rnf-Nqr [H]

EC number: NA

Molecular weight: Translated: 20894; Mature: 20763

Theoretical pI: Translated: 9.25; Mature: 9.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLATTFVMTLASICAWLID
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TWILIPLDLIYLRTLAFILVIAVVVQFTEMVVRKTSPALYRLLGIFLPLITTNCAVLGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNINLGHHFLQSALYGFSAAVGFSLVMVLFAAIRERLAVADVPAPFRGNAIALITAGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SLAFMGFSGLVKL
HHHHHHHHHHHCC
>Mature Secondary Structure 
TDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLATTFVMTLASICAWLID
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TWILIPLDLIYLRTLAFILVIAVVVQFTEMVVRKTSPALYRLLGIFLPLITTNCAVLGVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNINLGHHFLQSALYGFSAAVGFSLVMVLFAAIRERLAVADVPAPFRGNAIALITAGLM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SLAFMGFSGLVKL
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA