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The map label for this gene is dgoD [H]

Identifier: 29141105

GI number: 29141105

Start: 669092

End: 670294

Strand: Direct

Name: dgoD [H]

Synonym: t0591

Alternate gene names: 29141105

Gene position: 669092-670294 (Clockwise)

Preceding gene: 29141099

Following gene: 29141106

Centisome position: 13.96

GC content: 43.06

Gene sequence:

>1203_bases
ATGAAAATTACGTCTATTGAAGTTTTTGACTGTGAATTAAAAAAAAGAGATCAAACGATGTCATCCTATAATCCAGTACT
TATCCGGGTGAATACCGACTCAGGATTAAGTGGTATTGGCGAAGTAGGACTGGCCTATGGCGCAGGCGCTAAAGCTGGCG
TAGGGATCATCAGAGATCTGGCGTCATTGATTGTGGGTGAAGATCCATTAAATATCGAAAAAATTTGGGAGTTCTTTTTC
AGAAAGACTTTTTGGGGAATGGGAGGCGGGAACGTTTTTTATGCCGGAATGAGCGCTATTGATATTGCTCTTTGGGATAT
CAAAGGTAAATATTTAGGTGTACCTGTTTATCAGCTCCTTGGCGGGAAAACTAATGAAAAATTAAGAACCTATGCTAGTC
AGTTACAATTTGGTTGGGGGGATAAACGCCAGATATTAGTGACACCTGAGGAATATGCTGAGGCGGCGCGTGCGGCTCTT
GACGATGGATATGATGCGATTAAAGTGGATCCACTCGAAATCGATCGTAATGGCGATGACTGTGTTTTTCAGAATAGAAA
TCGTAACTATTCAGGATTGTTACTGGCCGATCAATTAAAAATGGGGGAAGCGCGAATTGCCGCTATGCGTGAAGCAATGG
GGGATGATGCTGATATTATCGTAGAAATCCATTCTCTTCTCGGTACAAACTCAGCTATTCAATTTGCGAAGGCGATAGAA
AAGTATCGTATCTTTCTTTATGAAGAACCGAGCCATCCATTAAATTCTGATAATATGCAGAAAGTTTCCCGTTCAACAAC
GATTCCAATCGCCACTGGCGAGCGTTCCTACACAAGATGGGGATACAGGGAATTACTGGAGAAACAGTCTATTGCCGTAG
CGCAACCTGATTTGTGTCTCTGCGGCGGAATTACCGAAGGAAAGAAAATCTGTGACTATGCTAATATTTATGACACCACT
GTACAGGTGCATGTTTGCGGCGGTCCTGTTTCCACGGTAGCCGCGCTGCATATGGAAACGGCAATTCCCAACTTTATTAT
TCATGAACATCATACCAATGCGATGAAAGCATCTATTCGGGAACTTTGTACCCACGATTATCAGCCGGAAAATGGCTATT
ATGTCGCGCCGGAACAGCCCGGATTGGGTCAGGAATTAAACGATGAGGTAGTGAAAGAATACCTGGCCTATGTGATTAAA
TAG

Upstream 100 bases:

>100_bases
TCGCTGCGGTAGCCGTTTTGGCGTTAATGAAAATTGATGTGAAAAAAGAAAAAAGCCAACAAACAAATGTTGTATCACGA
ACCTGATAAATGGAGAAATT

Downstream 100 bases:

>100_bases
CATCTGGTGATACTGATTATGGTTTATGCCTCCCGGCATATCCCCTAAATAATTCGAGTTGCAGGATAAAATGGTTAACC
GTCTTGAACAGCGCTTGCGC

Product: MR-MLE-family protein

Products: NA

Alternate protein names: GalD [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MKITSIEVFDCELKKRDQTMSSYNPVLIRVNTDSGLSGIGEVGLAYGAGAKAGVGIIRDLASLIVGEDPLNIEKIWEFFF
RKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLGVPVYQLLGGKTNEKLRTYASQLQFGWGDKRQILVTPEEYAEAARAAL
DDGYDAIKVDPLEIDRNGDDCVFQNRNRNYSGLLLADQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFAKAIE
KYRIFLYEEPSHPLNSDNMQKVSRSTTIPIATGERSYTRWGYRELLEKQSIAVAQPDLCLCGGITEGKKICDYANIYDTT
VQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKASIRELCTHDYQPENGYYVAPEQPGLGQELNDEVVKEYLAYVIK

Sequences:

>Translated_400_residues
MKITSIEVFDCELKKRDQTMSSYNPVLIRVNTDSGLSGIGEVGLAYGAGAKAGVGIIRDLASLIVGEDPLNIEKIWEFFF
RKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLGVPVYQLLGGKTNEKLRTYASQLQFGWGDKRQILVTPEEYAEAARAAL
DDGYDAIKVDPLEIDRNGDDCVFQNRNRNYSGLLLADQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFAKAIE
KYRIFLYEEPSHPLNSDNMQKVSRSTTIPIATGERSYTRWGYRELLEKQSIAVAQPDLCLCGGITEGKKICDYANIYDTT
VQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKASIRELCTHDYQPENGYYVAPEQPGLGQELNDEVVKEYLAYVIK
>Mature_400_residues
MKITSIEVFDCELKKRDQTMSSYNPVLIRVNTDSGLSGIGEVGLAYGAGAKAGVGIIRDLASLIVGEDPLNIEKIWEFFF
RKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLGVPVYQLLGGKTNEKLRTYASQLQFGWGDKRQILVTPEEYAEAARAAL
DDGYDAIKVDPLEIDRNGDDCVFQNRNRNYSGLLLADQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFAKAIE
KYRIFLYEEPSHPLNSDNMQKVSRSTTIPIATGERSYTRWGYRELLEKQSIAVAQPDLCLCGGITEGKKICDYANIYDTT
VQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKASIRELCTHDYQPENGYYVAPEQPGLGQELNDEVVKEYLAYVIK

Specific function: Catalyzes the dehydration of D-galactonate to 2-keto-3- deoxy-D-galactonate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GalD subfamily [H]

Homologues:

Organism=Escherichia coli, GI48994953, Length=379, Percent_Identity=28.4960422163588, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI1787864, Length=414, Percent_Identity=28.2608695652174, Blast_Score=160, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.6 [H]

Molecular weight: Translated: 44402; Mature: 44402

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITSIEVFDCELKKRDQTMSSYNPVLIRVNTDSGLSGIGEVGLAYGAGAKAGVGIIRDL
CCEEEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHH
ASLIVGEDPLNIEKIWEFFFRKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLGVPVYQLL
HHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEECCCEECCHHHHHH
GGKTNEKLRTYASQLQFGWGDKRQILVTPEEYAEAARAALDDGYDAIKVDPLEIDRNGDD
CCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEECCEEECCCCCC
CVFQNRNRNYSGLLLADQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFAKAIE
CEEECCCCCCCEEEEEHHHHCCHHHHHHHHHHHCCCCHHHHEEHHHHCCCHHHHHHHHHH
KYRIFLYEEPSHPLNSDNMQKVSRSTTIPIATGERSYTRWGYRELLEKQSIAVAQPDLCL
HEEEEEEECCCCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEECCCCEEE
CGGITEGKKICDYANIYDTTVQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKASIR
ECCCCCCHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
ELCTHDYQPENGYYVAPEQPGLGQELNDEVVKEYLAYVIK
HHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKITSIEVFDCELKKRDQTMSSYNPVLIRVNTDSGLSGIGEVGLAYGAGAKAGVGIIRDL
CCEEEEEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCCCCCCHHHHHHHHH
ASLIVGEDPLNIEKIWEFFFRKTFWGMGGGNVFYAGMSAIDIALWDIKGKYLGVPVYQLL
HHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEEEEECCCEECCHHHHHH
GGKTNEKLRTYASQLQFGWGDKRQILVTPEEYAEAARAALDDGYDAIKVDPLEIDRNGDD
CCCCCHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHCCCCEEEECCEEECCCCCC
CVFQNRNRNYSGLLLADQLKMGEARIAAMREAMGDDADIIVEIHSLLGTNSAIQFAKAIE
CEEECCCCCCCEEEEEHHHHCCHHHHHHHHHHHCCCCHHHHEEHHHHCCCHHHHHHHHHH
KYRIFLYEEPSHPLNSDNMQKVSRSTTIPIATGERSYTRWGYRELLEKQSIAVAQPDLCL
HEEEEEEECCCCCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCEECCCCEEE
CGGITEGKKICDYANIYDTTVQVHVCGGPVSTVAALHMETAIPNFIIHEHHTNAMKASIR
ECCCCCCHHHHHHCCEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCHHHHHHHH
ELCTHDYQPENGYYVAPEQPGLGQELNDEVVKEYLAYVIK
HHHHCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA