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The map label for this gene is insQ [H]

Identifier: 29140644

GI number: 29140644

Start: 116022

End: 117230

Strand: Direct

Name: insQ [H]

Synonym: t0102

Alternate gene names: 29140644

Gene position: 116022-117230 (Clockwise)

Preceding gene: 29140639

Following gene: 29140645

Centisome position: 2.42

GC content: 48.97

Gene sequence:

>1209_bases
ATGATGCTAATCCTGAAAGCCTACAAATTCAGACTGGAACCAACGCCTGAACAGTCGCAGCGTTTGCGGCAGTTATGTGG
TTGCGCCCGTTTTGTCTGGAATTTAGGTCTTGCGGAGACAAAGCGCATCCTTGGTTCAGGCGAAAAGTTACCTTCGGCTT
TCGAGTTGAACAGGATGCTTACAGTGTGGAAAAAAATGCCGGGACACATCTTTTTACAGGATGCTTATACCGACAATCTG
CAACAAAAGTTGAAAGACCTGCATACTGCATGGAAACGTTGCTTTGATAAAAAGCTCGCAGCTAAGGCCCCGGTATGGAA
ACGAAAAAATGAGGGCAGAGACTCAATCCGTTTTGTGAACTTTGAGAAATATTGCCGCCTTGAAAATCGCAGAGTGAAGC
TACCGTCAGGTCTTGGGTGGGTAAAATTCCGGCAATCTCAACGTGTGAACGGTAAAATCAAAAATGCGACAATCAGTCAG
TTAGCGGGACAGTGGTATATCTCGTTTCAGGTTGAAGTTGAAACGGCAGAACCCAATCACACAAGCACAACGATAGTCGG
TCTGGACGCAGGAGTAACAAAACTCGCCACGCTTTCAGACGGTACGGTATACCAACCAGTCAACAGTTTTAAAGCAAGTC
AGCGCAAGCTGGCAACACTCCAGCGGCAGTTAAGCCGTAAAGTCAGATTCAGCAGTAACTGGCAGAAACAAAAGCGAAAA
GTCCAGCATCTGCACTCGCACATAGCCAATATCCGGCGCGACTACCTTCATAAAGTTACCAGTGAAATCAGCAAAAACCA
CGCGATGATAGTCATTGAGGATTTGAAGGTTAGTAACATGTCGAAATCGGCAAAAGGTACAGCAGAACAGCACGGACGAA
ACGTCAAGGCCAAATCAGGTCTGAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGAGTACAAA
CAGCTCTGGCGTGGCGGTCAGGTGCTTGCGGTTCCGCCAGCATACACAAGCCAGCGTTGCGCGTGCTGTGGTCATACCGC
GAAAGAAAATCGCCTGTCACAAAGTAAATTTGTGTGTCAGGCATGCGGATATACAGCGAACGCTGATGTAAACGGCGCTC
GTAACATTTTAGCGGCGGGGCACGCCGTTCTTGCCTGTGGAGGGATGGTGCAGTCAGGCCGCCCGTCTGAAACAGGAACC
AGACGCTGA

Upstream 100 bases:

>100_bases
AAGGAAGGCGGCATGTCTTGAACGATTAATTTTATGTTTTTGTATTGAAGTTGACCGTAAAAACAGTAACGTAGCAAACA
AATCTCCTACACACTGAGTG

Downstream 100 bases:

>100_bases
GCAATCAGTCGTCACCCACTAATGGGAATTCCCCACCTGAGTCACGAAGTGCGAAGGTGGGGGAGGATGTCAATGTTGTT
GTCGCAGTATCTGTTTTGCG

Product: IS element transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MMLILKAYKFRLEPTPEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRMLTVWKKMPGHIFLQDAYTDNL
QQKLKDLHTAWKRCFDKKLAAKAPVWKRKNEGRDSIRFVNFEKYCRLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQ
LAGQWYISFQVEVETAEPNHTSTTIVGLDAGVTKLATLSDGTVYQPVNSFKASQRKLATLQRQLSRKVRFSSNWQKQKRK
VQHLHSHIANIRRDYLHKVTSEISKNHAMIVIEDLKVSNMSKSAKGTAEQHGRNVKAKSGLNRSILDQGWYEMRRQLEYK
QLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFVCQACGYTANADVNGARNILAAGHAVLACGGMVQSGRPSETGT
RR

Sequences:

>Translated_402_residues
MMLILKAYKFRLEPTPEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRMLTVWKKMPGHIFLQDAYTDNL
QQKLKDLHTAWKRCFDKKLAAKAPVWKRKNEGRDSIRFVNFEKYCRLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQ
LAGQWYISFQVEVETAEPNHTSTTIVGLDAGVTKLATLSDGTVYQPVNSFKASQRKLATLQRQLSRKVRFSSNWQKQKRK
VQHLHSHIANIRRDYLHKVTSEISKNHAMIVIEDLKVSNMSKSAKGTAEQHGRNVKAKSGLNRSILDQGWYEMRRQLEYK
QLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFVCQACGYTANADVNGARNILAAGHAVLACGGMVQSGRPSETGT
RR
>Mature_402_residues
MMLILKAYKFRLEPTPEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRMLTVWKKMPGHIFLQDAYTDNL
QQKLKDLHTAWKRCFDKKLAAKAPVWKRKNEGRDSIRFVNFEKYCRLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQ
LAGQWYISFQVEVETAEPNHTSTTIVGLDAGVTKLATLSDGTVYQPVNSFKASQRKLATLQRQLSRKVRFSSNWQKQKRK
VQHLHSHIANIRRDYLHKVTSEISKNHAMIVIEDLKVSNMSKSAKGTAEQHGRNVKAKSGLNRSILDQGWYEMRRQLEYK
QLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFVCQACGYTANADVNGARNILAAGHAVLACGGMVQSGRPSETGT
RR

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG0675

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transposase 35 family [H]

Homologues:

Organism=Escherichia coli, GI288549314, Length=374, Percent_Identity=65.24064171123, Blast_Score=478, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001959
- InterPro:   IPR010095
- InterPro:   IPR021027 [H]

Pfam domain/function: PF12323 HTH_14; PF01385 Transposase_2; PF07282 Transposase_35 [H]

EC number: NA

Molecular weight: Translated: 45660; Mature: 45660

Theoretical pI: Translated: 10.88; Mature: 10.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLILKAYKFRLEPTPEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRML
CEEEEEEHHEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHH
TVWKKMPGHIFLQDAYTDNLQQKLKDLHTAWKRCFDKKLAAKAPVWKRKNEGRDSIRFVN
HHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEEC
FEKYCRLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQLAGQWYISFQVEVETAEPNH
HHHHHHHCCCEEECCCCCCHHHHHCCCCCCCEECCHHHHHHCCCEEEEEEEEEEECCCCC
TSTTIVGLDAGVTKLATLSDGTVYQPVNSFKASQRKLATLQRQLSRKVRFSSNWQKQKRK
CCEEEEEECCCCHHEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VQHLHSHIANIRRDYLHKVTSEISKNHAMIVIEDLKVSNMSKSAKGTAEQHGRNVKAKSG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHCC
LNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFVCQ
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHH
ACGYTANADVNGARNILAAGHAVLACGGMVQSGRPSETGTRR
HCCCCCCCCCCHHHHHHHHCHHHHHCCCHHCCCCCCCCCCCC
>Mature Secondary Structure
MMLILKAYKFRLEPTPEQSQRLRQLCGCARFVWNLGLAETKRILGSGEKLPSAFELNRML
CEEEEEEHHEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHHH
TVWKKMPGHIFLQDAYTDNLQQKLKDLHTAWKRCFDKKLAAKAPVWKRKNEGRDSIRFVN
HHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCEEEEC
FEKYCRLENRRVKLPSGLGWVKFRQSQRVNGKIKNATISQLAGQWYISFQVEVETAEPNH
HHHHHHHCCCEEECCCCCCHHHHHCCCCCCCEECCHHHHHHCCCEEEEEEEEEEECCCCC
TSTTIVGLDAGVTKLATLSDGTVYQPVNSFKASQRKLATLQRQLSRKVRFSSNWQKQKRK
CCEEEEEECCCCHHEEECCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VQHLHSHIANIRRDYLHKVTSEISKNHAMIVIEDLKVSNMSKSAKGTAEQHGRNVKAKSG
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCHHCC
LNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFVCQ
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHCCCCHHHHHHHHHHHHHH
ACGYTANADVNGARNILAAGHAVLACGGMVQSGRPSETGTRR
HCCCCCCCCCCHHHHHHHHCHHHHHCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]