Click here to switch to the map view.

The map label for this gene is ubiE

Identifier: 28379760

GI number: 28379760

Start: 3043575

End: 3044288

Strand: Direct

Name: ubiE

Synonym: lp_3431

Alternate gene names: 28379760

Gene position: 3043575-3044288 (Clockwise)

Preceding gene: 28379759

Following gene: 28379761

Centisome position: 92.0

GC content: 48.04

Gene sequence:

>714_bases
ATGGCAAATCGTTATTTACATAATGTCCAAGGTTTGTTCGACACCATCGCGCCGAACTATGATCGGATGAATAACATCAT
CAGTCTCGGAACGCATCGTCATTGGCGCAAGCAAACGATGGCCCAAATCCATTTAGCGTCTAACGCCCATGTTCTTGACC
TCTGTTGTGGTACGGGTGATTGGACGATCGCGCTGGCTAAAGAATTACAGGCACCCGGTGAAGTCATCGGATTAGACTTT
TCAGCCCCAATGTTAAAACTGGCACAACAAAAAGTGACCCAGCAACAGGTTGCCGATCGGGTCTGGTTGCGCCGCGGTAA
TGCGATGCATCTTCCGTTTAAAGACAACACCTTTGACCTCGTCACGATTGGCTTCGGACTCCGCAACTTGCCTGATAAAG
CCCAAGCTTTAACTGAAATTTATCGCGTCCTAAAACCGGGGGCTCGCTTGGTCTGCTTAGAGACGTCCCAACCCGATCAG
CCATTGATTAAACCAGTCTGGCAGTGGTATTTCACAAAAGTAGTCCCACTATTCGGGCGCTTATTCGCCCATCAATATCA
GGAATATTCCTATTTACAGGAAACCACTCGTCACTTTGCAAGCTATCAACAATTAGCAACGATGTTTCAGCAAGCAGGCT
TTCAAAATGTTCACTTCCAACGGTTCAACTTTGGGGCCGCAGCGGCTCACTTTGGGACTAAGGAGGCCAAGTAA

Upstream 100 bases:

>100_bases
AATATCGTAGCTTTATATTGATTATCATTCCTTTTCACGGTAACTGGCAACTAACATCTGCTATAGTAGTAAGTAAAGCA
ACTATCAGGAGGTATCATTT

Downstream 100 bases:

>100_bases
TTGGACGAACTGACCGCTTTCAATCAATGTCTGGCTTGGTTGACACACACCACCGCCTTGCCTGAGCACCTCGATGGCCT
GGTCCTTTGTGGCAACAGCC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MANRYLHNVQGLFDTIAPNYDRMNNIISLGTHRHWRKQTMAQIHLASNAHVLDLCCGTGDWTIALAKELQAPGEVIGLDF
SAPMLKLAQQKVTQQQVADRVWLRRGNAMHLPFKDNTFDLVTIGFGLRNLPDKAQALTEIYRVLKPGARLVCLETSQPDQ
PLIKPVWQWYFTKVVPLFGRLFAHQYQEYSYLQETTRHFASYQQLATMFQQAGFQNVHFQRFNFGAAAAHFGTKEAK

Sequences:

>Translated_237_residues
MANRYLHNVQGLFDTIAPNYDRMNNIISLGTHRHWRKQTMAQIHLASNAHVLDLCCGTGDWTIALAKELQAPGEVIGLDF
SAPMLKLAQQKVTQQQVADRVWLRRGNAMHLPFKDNTFDLVTIGFGLRNLPDKAQALTEIYRVLKPGARLVCLETSQPDQ
PLIKPVWQWYFTKVVPLFGRLFAHQYQEYSYLQETTRHFASYQQLATMFQQAGFQNVHFQRFNFGAAAAHFGTKEAK
>Mature_236_residues
ANRYLHNVQGLFDTIAPNYDRMNNIISLGTHRHWRKQTMAQIHLASNAHVLDLCCGTGDWTIALAKELQAPGEVIGLDFS
APMLKLAQQKVTQQQVADRVWLRRGNAMHLPFKDNTFDLVTIGFGLRNLPDKAQALTEIYRVLKPGARLVCLETSQPDQP
LIKPVWQWYFTKVVPLFGRLFAHQYQEYSYLQETTRHFASYQQLATMFQQAGFQNVHFQRFNFGAAAAHFGTKEAK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=258, Percent_Identity=34.4961240310078, Blast_Score=155, Evalue=2e-38,
Organism=Escherichia coli, GI48994983, Length=226, Percent_Identity=38.9380530973451, Blast_Score=167, Evalue=6e-43,
Organism=Escherichia coli, GI1786404, Length=98, Percent_Identity=34.6938775510204, Blast_Score=66, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17557039, Length=232, Percent_Identity=32.7586206896552, Blast_Score=147, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6323526, Length=241, Percent_Identity=29.8755186721992, Blast_Score=124, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24641702, Length=239, Percent_Identity=33.8912133891213, Blast_Score=140, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_LACPL (Q88SI6)

Other databases:

- EMBL:   AL935262
- RefSeq:   NP_786652.1
- ProteinModelPortal:   Q88SI6
- SMR:   Q88SI6
- GeneID:   1063688
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_3431
- NMPDR:   fig|220668.1.peg.2786
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- ProtClustDB:   PRK00216
- BioCyc:   LPLA220668:LP_3431-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 27150; Mature: 27019

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRYLHNVQGLFDTIAPNYDRMNNIISLGTHRHWRKQTMAQIHLASNAHVLDLCCGTGD
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCC
WTIALAKELQAPGEVIGLDFSAPMLKLAQQKVTQQQVADRVWLRRGNAMHLPFKDNTFDL
HHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEE
VTIGFGLRNLPDKAQALTEIYRVLKPGARLVCLETSQPDQPLIKPVWQWYFTKVVPLFGR
EEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
LFAHQYQEYSYLQETTRHFASYQQLATMFQQAGFQNVHFQRFNFGAAAAHFGTKEAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCCC
>Mature Secondary Structure 
ANRYLHNVQGLFDTIAPNYDRMNNIISLGTHRHWRKQTMAQIHLASNAHVLDLCCGTGD
CCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCC
WTIALAKELQAPGEVIGLDFSAPMLKLAQQKVTQQQVADRVWLRRGNAMHLPFKDNTFDL
HHHHHHHHHCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCEEE
VTIGFGLRNLPDKAQALTEIYRVLKPGARLVCLETSQPDQPLIKPVWQWYFTKVVPLFGR
EEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHH
LFAHQYQEYSYLQETTRHFASYQQLATMFQQAGFQNVHFQRFNFGAAAAHFGTKEAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566