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The map label for this gene is gadB [H]

Identifier: 28379751

GI number: 28379751

Start: 3035469

End: 3036878

Strand: Direct

Name: gadB [H]

Synonym: lp_3420

Alternate gene names: 28379751

Gene position: 3035469-3036878 (Clockwise)

Preceding gene: 28379750

Following gene: 28379754

Centisome position: 91.75

GC content: 47.38

Gene sequence:

>1410_bases
ATGGCAATGTTATACGGTAAACACAATCATGAAGCTGAAGAATACTTGGAACCAGTCTTTGGTGCGCCTTCTGAACAACA
TGATCTTCCTAAGTATCGGTTACCAAAGCATTCATTATCCCCTCGAGAAGCCGATCGCTTAGTTCGTGATGAATTATTAG
ATGAAGGCAATTCACGACTGAACCTGGCAACTTTTTGTCAGACCTATATGGAACCCGAAGCCGTTGAATTGATGAAGGAT
ACGCTGGCTAAGAATGCCATCGACAAATCTGAGTACCCCCGCACGGCCGAGATTGAAAATCGGTGTGTGAACATTATTGC
CAATCTGTGGCACGCACCTGATGACGAACACTTTACGGGTACCTCTACGATTGGCTCCTCTGAAGCTTGTATGTTAGGCG
GTTTAGCAATGAAATTCGCCTGGCGTAAACGCGCTCAAGCGGCAGGTTTAGATCTGAATGCCCATCGACCTAACCTCGTT
ATTTCGGCTGGCTATCAAGTTTGCTGGGAAAAGTTTTGTGTCTACTGGGACGTTGACATGCACGTGGTCCCAATGGATGA
GCAACACATGGCCCTTGACGTTAACCACGTCTTAGACTACGTGGACGAATACACAATTGGTATCGTCGGTATCATGGGCA
TCACTTATACCGGTCAATATGACGACCTAGCCGCACTCGATAAGGTCGTTACTCACTACAATCATCAGCATCCCAAATTA
CCAGTCTACATTCACGTCGACGCAGCGTCAGGTGGCTTCTATACCCCATTTATTGAGCCGCAACTCATCTGGGACTTCCG
GTTGGCTAACGTCGTTTCGATCAACGCCTCCGGGCACAAGTACGGTTTAGTTTATCCCGGGGTCGGCTGGGTCGTTTGGC
GTGATCGTCAGTTTTTACCGCCAGAATTAGTCTTCAAAGTTAGTTATTTAGGTGGGGAGTTGCCGACAATGGCGATCAAC
TTCTCACATAGTGCAGCCCAGCTCATTGGACAATACTATAATTTCATTCGCTTTGGTATGGACGGTTACCGCGAGATTCA
AACAAAGACTCACGATGTTGCCCGCTACCTGGCAGCCGCTCTGGATAAAGTTGGTGAGTTTAAGATGATCAATAACGGAC
ACCAACTCCCCCTGATTTGTTACCAACTAGCCTCGCGCGAAGATCGTGAATGGACCCTTTATGATTTATCGGATCGCCTA
TTAATGAACGGTTGGCAAGTACCAACGTATCCTTTACCTGCTAATCTGGAACAACAAGTCATCCAACGAATCGTCGTTCG
GGCTGACTTTGGCATGAATATGGCCCACGATTTCATGGATGACCTGACCAAGGCTGTCCATGACTTAAACCACGCCCACA
TTGTCTATCATCATGACGCGGCACCTAAGAAATACGGATTCACACACTGA

Upstream 100 bases:

>100_bases
CCAGTTTTTTTGTTACCTACCATCCAGATTGCGTTTTAATTTACCCTGTTATCACCTCGTGCTATACTCGGCACAACTTA
TAAAGATAAGGGTGACCAAC

Downstream 100 bases:

>100_bases
ATCATCACGTTATTTTGATAAAATAGTTAAACTTCAGAAAACAATTATTATCACTCGTGTATACAAAAAATAGCACCATC
CAGATTCTGACGAACCGGGA

Product: glutamate decarboxylase

Products: NA

Alternate protein names: GAD [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MAMLYGKHNHEAEEYLEPVFGAPSEQHDLPKYRLPKHSLSPREADRLVRDELLDEGNSRLNLATFCQTYMEPEAVELMKD
TLAKNAIDKSEYPRTAEIENRCVNIIANLWHAPDDEHFTGTSTIGSSEACMLGGLAMKFAWRKRAQAAGLDLNAHRPNLV
ISAGYQVCWEKFCVYWDVDMHVVPMDEQHMALDVNHVLDYVDEYTIGIVGIMGITYTGQYDDLAALDKVVTHYNHQHPKL
PVYIHVDAASGGFYTPFIEPQLIWDFRLANVVSINASGHKYGLVYPGVGWVVWRDRQFLPPELVFKVSYLGGELPTMAIN
FSHSAAQLIGQYYNFIRFGMDGYREIQTKTHDVARYLAAALDKVGEFKMINNGHQLPLICYQLASREDREWTLYDLSDRL
LMNGWQVPTYPLPANLEQQVIQRIVVRADFGMNMAHDFMDDLTKAVHDLNHAHIVYHHDAAPKKYGFTH

Sequences:

>Translated_469_residues
MAMLYGKHNHEAEEYLEPVFGAPSEQHDLPKYRLPKHSLSPREADRLVRDELLDEGNSRLNLATFCQTYMEPEAVELMKD
TLAKNAIDKSEYPRTAEIENRCVNIIANLWHAPDDEHFTGTSTIGSSEACMLGGLAMKFAWRKRAQAAGLDLNAHRPNLV
ISAGYQVCWEKFCVYWDVDMHVVPMDEQHMALDVNHVLDYVDEYTIGIVGIMGITYTGQYDDLAALDKVVTHYNHQHPKL
PVYIHVDAASGGFYTPFIEPQLIWDFRLANVVSINASGHKYGLVYPGVGWVVWRDRQFLPPELVFKVSYLGGELPTMAIN
FSHSAAQLIGQYYNFIRFGMDGYREIQTKTHDVARYLAAALDKVGEFKMINNGHQLPLICYQLASREDREWTLYDLSDRL
LMNGWQVPTYPLPANLEQQVIQRIVVRADFGMNMAHDFMDDLTKAVHDLNHAHIVYHHDAAPKKYGFTH
>Mature_468_residues
AMLYGKHNHEAEEYLEPVFGAPSEQHDLPKYRLPKHSLSPREADRLVRDELLDEGNSRLNLATFCQTYMEPEAVELMKDT
LAKNAIDKSEYPRTAEIENRCVNIIANLWHAPDDEHFTGTSTIGSSEACMLGGLAMKFAWRKRAQAAGLDLNAHRPNLVI
SAGYQVCWEKFCVYWDVDMHVVPMDEQHMALDVNHVLDYVDEYTIGIVGIMGITYTGQYDDLAALDKVVTHYNHQHPKLP
VYIHVDAASGGFYTPFIEPQLIWDFRLANVVSINASGHKYGLVYPGVGWVVWRDRQFLPPELVFKVSYLGGELPTMAINF
SHSAAQLIGQYYNFIRFGMDGYREIQTKTHDVARYLAAALDKVGEFKMINNGHQLPLICYQLASREDREWTLYDLSDRLL
MNGWQVPTYPLPANLEQQVIQRIVVRADFGMNMAHDFMDDLTKAVHDLNHAHIVYHHDAAPKKYGFTH

Specific function: Converts internalized glutamate to GABA and increases the internal pH. Involved in glutamate-dependent acid resistance [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=362, Percent_Identity=23.4806629834254, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1787769, Length=442, Percent_Identity=44.7963800904977, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1789934, Length=442, Percent_Identity=44.5701357466063, Blast_Score=392, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17543922, Length=329, Percent_Identity=22.4924012158055, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25148342, Length=371, Percent_Identity=22.6415094339623, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17557272, Length=402, Percent_Identity=22.8855721393035, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323905, Length=475, Percent_Identity=36.4210526315789, Blast_Score=293, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6320500, Length=339, Percent_Identity=22.1238938053097, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355963, Length=397, Percent_Identity=24.4332493702771, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24654344, Length=397, Percent_Identity=24.4332493702771, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 53575; Mature: 53443

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMLYGKHNHEAEEYLEPVFGAPSEQHDLPKYRLPKHSLSPREADRLVRDELLDEGNSRL
CCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
NLATFCQTYMEPEAVELMKDTLAKNAIDKSEYPRTAEIENRCVNIIANLWHAPDDEHFTG
HHHHHHHHHCCHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TSTIGSSEACMLGGLAMKFAWRKRAQAAGLDLNAHRPNLVISAGYQVCWEKFCVYWDVDM
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHEEEEEECCE
HVVPMDEQHMALDVNHVLDYVDEYTIGIVGIMGITYTGQYDDLAALDKVVTHYNHQHPKL
EEECCCCCCCEEEHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
PVYIHVDAASGGFYTPFIEPQLIWDFRLANVVSINASGHKYGLVYPGVGWVVWRDRQFLP
EEEEEEECCCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCCEEEECCCCCCC
PELVFKVSYLGGELPTMAINFSHSAAQLIGQYYNFIRFGMDGYREIQTKTHDVARYLAAA
HHHHEEEHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LDKVGEFKMINNGHQLPLICYQLASREDREWTLYDLSDRLLMNGWQVPTYPLPANLEQQV
HHHCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCCCCCCHHHHH
IQRIVVRADFGMNMAHDFMDDLTKAVHDLNHAHIVYHHDAAPKKYGFTH
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AMLYGKHNHEAEEYLEPVFGAPSEQHDLPKYRLPKHSLSPREADRLVRDELLDEGNSRL
CEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCC
NLATFCQTYMEPEAVELMKDTLAKNAIDKSEYPRTAEIENRCVNIIANLWHAPDDEHFTG
HHHHHHHHHCCHHHHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
TSTIGSSEACMLGGLAMKFAWRKRAQAAGLDLNAHRPNLVISAGYQVCWEKFCVYWDVDM
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHHHHHHEEEEEECCE
HVVPMDEQHMALDVNHVLDYVDEYTIGIVGIMGITYTGQYDDLAALDKVVTHYNHQHPKL
EEECCCCCCCEEEHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCC
PVYIHVDAASGGFYTPFIEPQLIWDFRLANVVSINASGHKYGLVYPGVGWVVWRDRQFLP
EEEEEEECCCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEEEEECCCCEEEECCCCCCC
PELVFKVSYLGGELPTMAINFSHSAAQLIGQYYNFIRFGMDGYREIQTKTHDVARYLAAA
HHHHEEEHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LDKVGEFKMINNGHQLPLICYQLASREDREWTLYDLSDRLLMNGWQVPTYPLPANLEQQV
HHHCCCEEEECCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCCCCCCHHHHH
IQRIVVRADFGMNMAHDFMDDLTKAVHDLNHAHIVYHHDAAPKKYGFTH
HHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10411264; 11337471 [H]