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The map label for this gene is gshR4 [H]

Identifier: 28379636

GI number: 28379636

Start: 2906700

End: 2908034

Strand: Direct

Name: gshR4 [H]

Synonym: lp_3267

Alternate gene names: 28379636

Gene position: 2906700-2908034 (Clockwise)

Preceding gene: 28379634

Following gene: 28379637

Centisome position: 87.86

GC content: 48.39

Gene sequence:

>1335_bases
ATGACAAACAAATACGATTACGATGTGTTATACATTGGTGCGGGTCACGCCACGTTTGATGGCGCCGCACCACTCGCCAA
GACTGGTGTTCGCGTCGGTGTGATTGAGAGTGGTCTGATTGGGGGCACCTGCCCTAATCGTGGTTGTAACGCCAAAATCA
CTCTTGATGAACCTGTCAAATTAACGCGGGAAACAGCACGCCTCAATGATATTTTGAGCAGTGCACCAACCATTAATTGG
ACGGCCAACGTGGCCCATAAACAAGAGATTATTGATCCCTTACCAGCAGGCCTGACAGCTCGTTTGGAAGATGGTGGCGC
AACGATCATTCATGGTCATGCCACGTTCAAGGACGCTCACACCGTTGTCGTTGATGATCAGCAAACCATTACCGCTGAAA
AAATCGTTATTGCGACTGGTTTAAAGCCCCATCGTTTAGATATTCCGGGGACCAAGCTCGCCCACGATAGTAGTGATTTT
ATGAACCTTAAACGGTTACCACAAAGTATCGTCATCATTGGCGCAGGCTATATTGGTATGGAATTTGCCACCATCGCTAA
CGCGGCCGGTGCCCAAGTGACAGTCATGTTACATGGCGACCAGGCCCTCCGCGATTTTTACCAACCATTCGTTGCACAAG
TGGTTGACGACTTAACCGAACGTGGGGTAACCTTCATCAAAAATGCCAACGTGCAAGCATTTACCAAACAGGATGACCAG
TTCCAAGTTAGTTATGGCGACCACCAGCAATTAACGACCGACTGGATCCTAGATGCGACTGGTCGAATTCCAAACTTAGA
TGGCTTAGGCTTAGACCGGATTGGCGTCAAATATGACCGCCACGGCGTTTATGTCAACGACCACTTACAAACCAACGTTC
CTAACATCTATGCAGCCGGTGATGTCCTTGCCAATGACTTGCCAAAGGTCACACCTGCAGCCTACTTTGAGTCCAAATAC
TTGATGCGTTTGTTCTCTGGTCAAACCAGTGCTCCCATTGACTATCCGGTAATTCCATCGGTTGTCTTTACTTCACCACG
AATTGCGCAAGCTGGCATGAAAATACCGGCCGCTGAAAAAGCCGGCTTAACCGTTAGTGATAATGATTTAGCAGATTACT
GGTATTATCAAGTCTCCAAGGAACCGATTGCCGCTAGTAAACAGGTCCATGACCAAGACGGCCATCTCGTTGGCGTGACT
GAAATCAGTGATCAAGCTGAAGATGCCGTCAACGCACTACTGCCAGCTATCGAATATCAATTAGACCGTGAACAAATCGA
CCGTTTGATCGGTATTTTCCCAACCATCGGCTACGCAGCTTGGCACCGGGCTTAA

Upstream 100 bases:

>100_bases
ATAATCATTACAGCGGGCGCGAGCAATTCATATCAGTTGTCAGGTTTCTTAAAACTCGTTAAGCTTAAGTTACCAAAATT
TTAATGGAGGCTGATAAATC

Downstream 100 bases:

>100_bases
AACAGAAAACACTGCTGCGACCGCCGCTTGATTAATCCCTTATTAATAACCAGAGCCGCCAATGACCTCCCACTCATTGG
CGGCTCTGGTTATGTTCGTT

Product: glutathione reductase

Products: NA

Alternate protein names: GR; GRase [H]

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MTNKYDYDVLYIGAGHATFDGAAPLAKTGVRVGVIESGLIGGTCPNRGCNAKITLDEPVKLTRETARLNDILSSAPTINW
TANVAHKQEIIDPLPAGLTARLEDGGATIIHGHATFKDAHTVVVDDQQTITAEKIVIATGLKPHRLDIPGTKLAHDSSDF
MNLKRLPQSIVIIGAGYIGMEFATIANAAGAQVTVMLHGDQALRDFYQPFVAQVVDDLTERGVTFIKNANVQAFTKQDDQ
FQVSYGDHQQLTTDWILDATGRIPNLDGLGLDRIGVKYDRHGVYVNDHLQTNVPNIYAAGDVLANDLPKVTPAAYFESKY
LMRLFSGQTSAPIDYPVIPSVVFTSPRIAQAGMKIPAAEKAGLTVSDNDLADYWYYQVSKEPIAASKQVHDQDGHLVGVT
EISDQAEDAVNALLPAIEYQLDREQIDRLIGIFPTIGYAAWHRA

Sequences:

>Translated_444_residues
MTNKYDYDVLYIGAGHATFDGAAPLAKTGVRVGVIESGLIGGTCPNRGCNAKITLDEPVKLTRETARLNDILSSAPTINW
TANVAHKQEIIDPLPAGLTARLEDGGATIIHGHATFKDAHTVVVDDQQTITAEKIVIATGLKPHRLDIPGTKLAHDSSDF
MNLKRLPQSIVIIGAGYIGMEFATIANAAGAQVTVMLHGDQALRDFYQPFVAQVVDDLTERGVTFIKNANVQAFTKQDDQ
FQVSYGDHQQLTTDWILDATGRIPNLDGLGLDRIGVKYDRHGVYVNDHLQTNVPNIYAAGDVLANDLPKVTPAAYFESKY
LMRLFSGQTSAPIDYPVIPSVVFTSPRIAQAGMKIPAAEKAGLTVSDNDLADYWYYQVSKEPIAASKQVHDQDGHLVGVT
EISDQAEDAVNALLPAIEYQLDREQIDRLIGIFPTIGYAAWHRA
>Mature_443_residues
TNKYDYDVLYIGAGHATFDGAAPLAKTGVRVGVIESGLIGGTCPNRGCNAKITLDEPVKLTRETARLNDILSSAPTINWT
ANVAHKQEIIDPLPAGLTARLEDGGATIIHGHATFKDAHTVVVDDQQTITAEKIVIATGLKPHRLDIPGTKLAHDSSDFM
NLKRLPQSIVIIGAGYIGMEFATIANAAGAQVTVMLHGDQALRDFYQPFVAQVVDDLTERGVTFIKNANVQAFTKQDDQF
QVSYGDHQQLTTDWILDATGRIPNLDGLGLDRIGVKYDRHGVYVNDHLQTNVPNIYAAGDVLANDLPKVTPAAYFESKYL
MRLFSGQTSAPIDYPVIPSVVFTSPRIAQAGMKIPAAEKAGLTVSDNDLADYWYYQVSKEPIAASKQVHDQDGHLVGVTE
ISDQAEDAVNALLPAIEYQLDREQIDRLIGIFPTIGYAAWHRA

Specific function: Maintains high levels of reduced glutathione in the cytosol [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI50301238, Length=459, Percent_Identity=29.6296296296296, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI291045266, Length=478, Percent_Identity=27.8242677824268, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI91199540, Length=470, Percent_Identity=28.2978723404255, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI22035672, Length=338, Percent_Identity=28.9940828402367, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI148277065, Length=434, Percent_Identity=26.2672811059908, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519430, Length=434, Percent_Identity=26.2672811059908, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519428, Length=434, Percent_Identity=26.2672811059908, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI33519426, Length=434, Percent_Identity=26.2672811059908, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI148277071, Length=434, Percent_Identity=26.2672811059908, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI291045268, Length=468, Percent_Identity=26.7094017094017, Blast_Score=109, Evalue=6e-24,
Organism=Escherichia coli, GI1789915, Length=448, Percent_Identity=29.6875, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1786307, Length=467, Percent_Identity=25.2676659528908, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI87082354, Length=376, Percent_Identity=29.5212765957447, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081717, Length=370, Percent_Identity=29.4594594594595, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789065, Length=193, Percent_Identity=29.0155440414508, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71983429, Length=422, Percent_Identity=29.6208530805687, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71983419, Length=422, Percent_Identity=29.6208530805687, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI32565766, Length=473, Percent_Identity=27.061310782241, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17557007, Length=378, Percent_Identity=27.2486772486773, Blast_Score=112, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=447, Percent_Identity=25.503355704698, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17559934, Length=201, Percent_Identity=27.363184079602, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325166, Length=461, Percent_Identity=27.114967462039, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6321091, Length=489, Percent_Identity=23.3128834355828, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6325240, Length=321, Percent_Identity=25.5451713395639, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24640553, Length=379, Percent_Identity=30.6068601583113, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24640549, Length=379, Percent_Identity=30.6068601583113, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24640551, Length=471, Percent_Identity=28.0254777070064, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17737741, Length=477, Percent_Identity=27.6729559748428, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI21358499, Length=477, Percent_Identity=27.0440251572327, Blast_Score=127, Evalue=1e-29,

Paralogues:

None

Copy number: 650 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.7 [H]

Molecular weight: Translated: 48259; Mature: 48128

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNKYDYDVLYIGAGHATFDGAAPLAKTGVRVGVIESGLIGGTCPNRGCNAKITLDEPVK
CCCCEEEEEEEEECCCCCCCCCCCHHHCCEEEEEEECCCCCCCCCCCCCCEEEEECCCHH
LTRETARLNDILSSAPTINWTANVAHKQEIIDPLPAGLTARLEDGGATIIHGHATFKDAH
HHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCEEEEECCEEECCCE
TVVVDDQQTITAEKIVIATGLKPHRLDIPGTKLAHDSSDFMNLKRLPQSIVIIGAGYIGM
EEEECCCCEEEEEEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCHHHH
EFATIANAAGAQVTVMLHGDQALRDFYQPFVAQVVDDLTERGVTFIKNANVQAFTKQDDQ
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCE
FQVSYGDHQQLTTDWILDATGRIPNLDGLGLDRIGVKYDRHGVYVNDHLQTNVPNIYAAG
EEEECCCCCCCCHHHEEECCCCCCCCCCCCCHHHCEEECCCCEEECCCCCCCCCCEEECC
DVLANDLPKVTPAAYFESKYLMRLFSGQTSAPIDYPVIPSVVFTSPRIAQAGMKIPAAEK
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHHCCCCCCCCCC
AGLTVSDNDLADYWYYQVSKEPIAASKQVHDQDGHLVGVTEISDQAEDAVNALLPAIEYQ
CCCEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
LDREQIDRLIGIFPTIGYAAWHRA
CCHHHHHHHHHHHHHHCHHHHCCC
>Mature Secondary Structure 
TNKYDYDVLYIGAGHATFDGAAPLAKTGVRVGVIESGLIGGTCPNRGCNAKITLDEPVK
CCCEEEEEEEEECCCCCCCCCCCHHHCCEEEEEEECCCCCCCCCCCCCCEEEEECCCHH
LTRETARLNDILSSAPTINWTANVAHKQEIIDPLPAGLTARLEDGGATIIHGHATFKDAH
HHHHHHHHHHHHHCCCCCEEEECCHHHHHHHCCCCCCCEEEEECCCEEEEECCEEECCCE
TVVVDDQQTITAEKIVIATGLKPHRLDIPGTKLAHDSSDFMNLKRLPQSIVIIGAGYIGM
EEEECCCCEEEEEEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHCCCEEEEEECCHHHH
EFATIANAAGAQVTVMLHGDQALRDFYQPFVAQVVDDLTERGVTFIKNANVQAFTKQDDQ
HHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCCE
FQVSYGDHQQLTTDWILDATGRIPNLDGLGLDRIGVKYDRHGVYVNDHLQTNVPNIYAAG
EEEECCCCCCCCHHHEEECCCCCCCCCCCCCHHHCEEECCCCEEECCCCCCCCCCEEECC
DVLANDLPKVTPAAYFESKYLMRLFSGQTSAPIDYPVIPSVVFTSPRIAQAGMKIPAAEK
HHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCHHHCCCCCCCCCC
AGLTVSDNDLADYWYYQVSKEPIAASKQVHDQDGHLVGVTEISDQAEDAVNALLPAIEYQ
CCCEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
LDREQIDRLIGIFPTIGYAAWHRA
CCHHHHHHHHHHHHHHCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1849605; 10984043 [H]