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The map label for this gene is fliY [C]

Identifier: 28379594

GI number: 28379594

Start: 2862968

End: 2863783

Strand: Direct

Name: fliY [C]

Synonym: lp_3214

Alternate gene names: 28379594

Gene position: 2862968-2863783 (Clockwise)

Preceding gene: 28379593

Following gene: 28379596

Centisome position: 86.54

GC content: 44.98

Gene sequence:

>816_bases
ATGAAAAAATGGCTCAAAGCAGTAAGTTTAACAATTTTAGGACTCGCACTAACAGTCGCCTTGACGGCATGCGGTAATTC
TAGCTCATCTAAAAGCAGCACTTCTAGCACAAGTAGTCAGCTCAAATTGCAAAAGAGCGACACGTTGACGATCGGGCTAG
AAGGCACCTTCCAGCCTTATAGCTATCGCAAAGACGGTAAGTTGACTGGTTTTGAAGTCGACCTTGGTAAAGCCGTCGCT
AAAAAGCTCGGTCTCAAGGCAAAGTTCGTCCCAACGAAATTCGACTCCTTGATTGCTGGCCTGGATGTCGACAAATACGA
CGTGGTCATCAACGATATCGCTGAAACTGCGCAACGTAAACAAAAATACCTCTTCTCTACGCCTTATATCTACTCTAAAT
CCGAATTAGCGGTCAAGAAGGGGTCCTCAATTAACAAGATCACGGATATCAAAGGCAAGAAAGTTGCCCAAACAACGACT
AGTAATAACGCGACCGATGCTAAACGTTTAGGTGCGACCGTCACACCGACCGATGGCTTTCAACAATCGATCGACCTCGT
AAACCAAGGTCGCGTCGCCGGAACGATCAACTCACGGGAAGCGTTCTATGCTTACCTCAAGCAGAATCCAAAGGCCAGCT
TGAAATTCATCAGTGGTGGTTCAGAAATCAAAACACAAAAGATTGGCGCAATTGTCACTAAAAAGCACGCCCCACTCCAA
AAACAACTGTCTAAAGCGATTCAAGAACTCCGTCAAGACGGCACCTTAAAGAAACTGTCTAATAAATACTTTGGTGGTAA
CGTCACAAACAAATAA

Upstream 100 bases:

>100_bases
CGCTTGGAAACTAAGGTGGTACCACGATCAGCAGTCGTCCTTTTTCGATAAAGGACGACTTTTTTTGTCAAAAAAATATA
GCTCTACGGGGGTAATCGAT

Downstream 100 bases:

>100_bases
TCATCGCAATCTATTTCATAGCGTCCAGTTAATAGTCACAAAAAGCCCGGTTTTCCGGGCTTTTTTGTGTATCGGCGTCT
TTCAGCGACCATCTTTTATC

Product: amino acid ABC transporter, substrate binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKKWLKAVSLTILGLALTVALTACGNSSSSKSSTSSTSSQLKLQKSDTLTIGLEGTFQPYSYRKDGKLTGFEVDLGKAVA
KKLGLKAKFVPTKFDSLIAGLDVDKYDVVINDIAETAQRKQKYLFSTPYIYSKSELAVKKGSSINKITDIKGKKVAQTTT
SNNATDAKRLGATVTPTDGFQQSIDLVNQGRVAGTINSREAFYAYLKQNPKASLKFISGGSEIKTQKIGAIVTKKHAPLQ
KQLSKAIQELRQDGTLKKLSNKYFGGNVTNK

Sequences:

>Translated_271_residues
MKKWLKAVSLTILGLALTVALTACGNSSSSKSSTSSTSSQLKLQKSDTLTIGLEGTFQPYSYRKDGKLTGFEVDLGKAVA
KKLGLKAKFVPTKFDSLIAGLDVDKYDVVINDIAETAQRKQKYLFSTPYIYSKSELAVKKGSSINKITDIKGKKVAQTTT
SNNATDAKRLGATVTPTDGFQQSIDLVNQGRVAGTINSREAFYAYLKQNPKASLKFISGGSEIKTQKIGAIVTKKHAPLQ
KQLSKAIQELRQDGTLKKLSNKYFGGNVTNK
>Mature_271_residues
MKKWLKAVSLTILGLALTVALTACGNSSSSKSSTSSTSSQLKLQKSDTLTIGLEGTFQPYSYRKDGKLTGFEVDLGKAVA
KKLGLKAKFVPTKFDSLIAGLDVDKYDVVINDIAETAQRKQKYLFSTPYIYSKSELAVKKGSSINKITDIKGKKVAQTTT
SNNATDAKRLGATVTPTDGFQQSIDLVNQGRVAGTINSREAFYAYLKQNPKASLKFISGGSEIKTQKIGAIVTKKHAPLQ
KQLSKAIQELRQDGTLKKLSNKYFGGNVTNK

Specific function: Probably part of a binding-protein-dependent transport system for an amino acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=233, Percent_Identity=37.3390557939914, Blast_Score=134, Evalue=7e-33,
Organism=Escherichia coli, GI1788649, Length=276, Percent_Identity=30.7971014492754, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787088, Length=228, Percent_Identity=28.9473684210526, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787085, Length=234, Percent_Identity=28.6324786324786, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788648, Length=234, Percent_Identity=29.9145299145299, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787031, Length=224, Percent_Identity=25, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 29330; Mature: 29330

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLKAVSLTILGLALTVALTACGNSSSSKSSTSSTSSQLKLQKSDTLTIGLEGTFQPY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEECCCEEEEEECCCCCCC
SYRKDGKLTGFEVDLGKAVAKKLGLKAKFVPTKFDSLIAGLDVDKYDVVINDIAETAQRK
CCCCCCCEEEEEEHHHHHHHHHHCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QKYLFSTPYIYSKSELAVKKGSSINKITDIKGKKVAQTTTSNNATDAKRLGATVTPTDGF
HHHEECCCCEECCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCH
QQSIDLVNQGRVAGTINSREAFYAYLKQNPKASLKFISGGSEIKTQKIGAIVTKKHAPLQ
HHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHEEECCCCCHH
KQLSKAIQELRQDGTLKKLSNKYFGGNVTNK
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKKWLKAVSLTILGLALTVALTACGNSSSSKSSTSSTSSQLKLQKSDTLTIGLEGTFQPY
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHEEEECCCEEEEEECCCCCCC
SYRKDGKLTGFEVDLGKAVAKKLGLKAKFVPTKFDSLIAGLDVDKYDVVINDIAETAQRK
CCCCCCCEEEEEEHHHHHHHHHHCCCEEECCCHHHHHHHCCCCCHHHHHHHHHHHHHHHH
QKYLFSTPYIYSKSELAVKKGSSINKITDIKGKKVAQTTTSNNATDAKRLGATVTPTDGF
HHHEECCCCEECCCHHHHHCCCCCCCCCCCCCCEEEEECCCCCCCHHHHCCCCCCCCCCH
QQSIDLVNQGRVAGTINSREAFYAYLKQNPKASLKFISGGSEIKTQKIGAIVTKKHAPLQ
HHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHEEECCCCCHH
KQLSKAIQELRQDGTLKKLSNKYFGGNVTNK
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]