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The map label for this gene is apbE2 [H]

Identifier: 28379385

GI number: 28379385

Start: 2615368

End: 2616411

Strand: Direct

Name: apbE2 [H]

Synonym: lp_2936

Alternate gene names: 28379385

Gene position: 2615368-2616411 (Clockwise)

Preceding gene: 28379384

Following gene: 28379389

Centisome position: 79.06

GC content: 50.38

Gene sequence:

>1044_bases
TTGCTGAACTTGTTGACATTTATGAAAGATAGGACTGGTTTTATGACAACTTTAGCTTATAATGGCGAGACAGCAATTCC
TGCACTCGCTCAGCAGCGTCGACACCAAGCTCATCACGCGCTCGGTACCGACATTGATTTGACCCTTTTTGGTAGTGATA
GTGACCAGTACCTACAAGCTGCTTGCGATCTTATCGACCAATATGAAGACCGCTTGACTGTCAACCGGACTGTTTCTGAG
GTCATGGCAATCAACCAGGCGGCGGGCCAACACCCCGTTCAAGTGAGCGCCGCCACCTATACACTCGTTCGGCAAGCTGT
CGAACTCAGTCGACAACATTTTGGTTTCAATGCGTTGATTGGTCCGCTCGTGAAACTCTGGCACATTGGTTTTACTAACG
CGCACGTCCCTGAACAGTCTGCAATTAACGAACGATTGGCCTTGATCGACCCCGAAGCCGTCATTTGTAATGACGCTGAT
TTCAGTGTGCTTCTCAAGGTCACGGGAATGGAGCTGGATCTAGGCGCAATTGCCAAGGGCTACATTGCCGACCGCATTCA
AGATTACTGGGAAGCATTTGGACAGCGGGCCGGAATGATCAACCTAGGTGGTAATCTGTTAATGGTGGGGACGTCCCCAC
TTCATCCGGATGGGCAATGGCGCGTTGGTATCCAAGATCCCACCACTGACCGCGGTAACGCGATTGCCAGCGTTACGATT
GGCCCCTGCTCGGCCGTGACCAGCGGTATTTACGAGCGGCACCTTGAATTTAACGGCCAGTCCTACCACCACATCTTAGA
TTCGCAAACGGGTTATCCCCGACAAAACGACCTTGAAAGTGTCACCGTTTTCTCGAAAGCTTCCATTGATGGTGAAATCG
AAACGACGCGGCTCTTCTTTGCGGGCCAACCGATTCCCGATTGGGCAACTCAGCGACCCGATATTTACGGCGCCATTTTT
GTGACTAAACAACGTGAGCTTCAAATCGTTGGCTTATCACCTGAACAAGTTACCTTACTCGATGACCGGTTTACAATAAT
TTAG

Upstream 100 bases:

>100_bases
CGACTTCACAGCACAAATTATTCAACACGAATTAGATCACTGTGCAGGGAAACTGATTTAATTGTGCCTACTTCTAACTG
AGCGGGAATAAATATTCAGT

Downstream 100 bases:

>100_bases
ACCACAAAATAAGCTAGGTCCTCCAAGAAATTAACTTCTTGAAGGACCTAGCTTATTTTATGGTCCACTTACAGGCTAGC
ATTTAATTTGCAGTTGACTT

Product: thiamin biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MLNLLTFMKDRTGFMTTLAYNGETAIPALAQQRRHQAHHALGTDIDLTLFGSDSDQYLQAACDLIDQYEDRLTVNRTVSE
VMAINQAAGQHPVQVSAATYTLVRQAVELSRQHFGFNALIGPLVKLWHIGFTNAHVPEQSAINERLALIDPEAVICNDAD
FSVLLKVTGMELDLGAIAKGYIADRIQDYWEAFGQRAGMINLGGNLLMVGTSPLHPDGQWRVGIQDPTTDRGNAIASVTI
GPCSAVTSGIYERHLEFNGQSYHHILDSQTGYPRQNDLESVTVFSKASIDGEIETTRLFFAGQPIPDWATQRPDIYGAIF
VTKQRELQIVGLSPEQVTLLDDRFTII

Sequences:

>Translated_347_residues
MLNLLTFMKDRTGFMTTLAYNGETAIPALAQQRRHQAHHALGTDIDLTLFGSDSDQYLQAACDLIDQYEDRLTVNRTVSE
VMAINQAAGQHPVQVSAATYTLVRQAVELSRQHFGFNALIGPLVKLWHIGFTNAHVPEQSAINERLALIDPEAVICNDAD
FSVLLKVTGMELDLGAIAKGYIADRIQDYWEAFGQRAGMINLGGNLLMVGTSPLHPDGQWRVGIQDPTTDRGNAIASVTI
GPCSAVTSGIYERHLEFNGQSYHHILDSQTGYPRQNDLESVTVFSKASIDGEIETTRLFFAGQPIPDWATQRPDIYGAIF
VTKQRELQIVGLSPEQVTLLDDRFTII
>Mature_347_residues
MLNLLTFMKDRTGFMTTLAYNGETAIPALAQQRRHQAHHALGTDIDLTLFGSDSDQYLQAACDLIDQYEDRLTVNRTVSE
VMAINQAAGQHPVQVSAATYTLVRQAVELSRQHFGFNALIGPLVKLWHIGFTNAHVPEQSAINERLALIDPEAVICNDAD
FSVLLKVTGMELDLGAIAKGYIADRIQDYWEAFGQRAGMINLGGNLLMVGTSPLHPDGQWRVGIQDPTTDRGNAIASVTI
GPCSAVTSGIYERHLEFNGQSYHHILDSQTGYPRQNDLESVTVFSKASIDGEIETTRLFFAGQPIPDWATQRPDIYGAIF
VTKQRELQIVGLSPEQVTLLDDRFTII

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=294, Percent_Identity=27.891156462585, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 38314; Mature: 38314

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNLLTFMKDRTGFMTTLAYNGETAIPALAQQRRHQAHHALGTDIDLTLFGSDSDQYLQA
CCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
ACDLIDQYEDRLTVNRTVSEVMAINQAAGQHPVQVSAATYTLVRQAVELSRQHFGFNALI
HHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHH
GPLVKLWHIGFTNAHVPEQSAINERLALIDPEAVICNDADFSVLLKVTGMELDLGAIAKG
HHHHHHHHCCCCCCCCCCHHHHHCCEEEECCCEEEECCCCEEEEEEEECCEECHHHHHHH
YIADRIQDYWEAFGQRAGMINLGGNLLMVGTSPLHPDGQWRVGIQDPTTDRGNAIASVTI
HHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCEEEECCCCCCCCCCEEEEEEE
GPCSAVTSGIYERHLEFNGQSYHHILDSQTGYPRQNDLESVTVFSKASIDGEIETTRLFF
CCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEE
AGQPIPDWATQRPDIYGAIFVTKQRELQIVGLSPEQVTLLDDRFTII
ECCCCCCHHCCCCCEEEEEEEEECCEEEEEECCCCEEEEECCCEEEC
>Mature Secondary Structure
MLNLLTFMKDRTGFMTTLAYNGETAIPALAQQRRHQAHHALGTDIDLTLFGSDSDQYLQA
CCCHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
ACDLIDQYEDRLTVNRTVSEVMAINQAAGQHPVQVSAATYTLVRQAVELSRQHFGFNALI
HHHHHHHHHHHEEHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHH
GPLVKLWHIGFTNAHVPEQSAINERLALIDPEAVICNDADFSVLLKVTGMELDLGAIAKG
HHHHHHHHCCCCCCCCCCHHHHHCCEEEECCCEEEECCCCEEEEEEEECCEECHHHHHHH
YIADRIQDYWEAFGQRAGMINLGGNLLMVGTSPLHPDGQWRVGIQDPTTDRGNAIASVTI
HHHHHHHHHHHHHHCCCCEEEECCCEEEEECCCCCCCCCEEEECCCCCCCCCCEEEEEEE
GPCSAVTSGIYERHLEFNGQSYHHILDSQTGYPRQNDLESVTVFSKASIDGEIETTRLFF
CCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEEE
AGQPIPDWATQRPDIYGAIFVTKQRELQIVGLSPEQVTLLDDRFTII
ECCCCCCHHCCCCCEEEEEEEEECCEEEEEECCCCEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]