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The map label for this gene is def2 [H]

Identifier: 28379384

GI number: 28379384

Start: 2614918

End: 2615328

Strand: Direct

Name: def2 [H]

Synonym: lp_2935

Alternate gene names: 28379384

Gene position: 2614918-2615328 (Clockwise)

Preceding gene: 28379383

Following gene: 28379385

Centisome position: 79.04

GC content: 45.74

Gene sequence:

>411_bases
ATGATTCGACCAATTGTGCATGATCCCGCCGCGCTTAGTGTCCCTGCTGACTTAGCAACCCCTGCAGACACTCAAGTCTT
GACTGATTTACTGGATACCCTCGCCGCACACACCGATAATTGCGTTGGAATGGCCGCGAATATGATTGGGGTCAACAAAC
AAATTATTGTCGTGCAACTCGGTCCCTTTGCAATTGCAATGATCAATCCGAAGATCATTGATCATCACGGCACTTACGAG
ACTAAGGAAGGCTGCCTGTCCTTACCTGGTGAACGTCCGACTAGCCGCTATCACCAAATCACGGTGAAATATAAAGATCA
ACACTTTAAACCGCAACAACAACGATTCAACGACTTCACAGCACAAATTATTCAACACGAATTAGATCACTGTGCAGGGA
AACTGATTTAA

Upstream 100 bases:

>100_bases
GGTGTGTTTTTTAGGGCCTGACAACTAGCGAACGGCTTGCTTCTGTCAACCGTGCTCTCTATAATTAAATTAATATGACT
AATCAATGGAGGAACATCAA

Downstream 100 bases:

>100_bases
TTGTGCCTACTTCTAACTGAGCGGGAATAAATATTCAGTTTGCTGAACTTGTTGACATTTATGAAAGATAGGACTGGTTT
TATGACAACTTTAGCTTATA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 136; Mature: 136

Protein sequence:

>136_residues
MIRPIVHDPAALSVPADLATPADTQVLTDLLDTLAAHTDNCVGMAANMIGVNKQIIVVQLGPFAIAMINPKIIDHHGTYE
TKEGCLSLPGERPTSRYHQITVKYKDQHFKPQQQRFNDFTAQIIQHELDHCAGKLI

Sequences:

>Translated_136_residues
MIRPIVHDPAALSVPADLATPADTQVLTDLLDTLAAHTDNCVGMAANMIGVNKQIIVVQLGPFAIAMINPKIIDHHGTYE
TKEGCLSLPGERPTSRYHQITVKYKDQHFKPQQQRFNDFTAQIIQHELDHCAGKLI
>Mature_136_residues
MIRPIVHDPAALSVPADLATPADTQVLTDLLDTLAAHTDNCVGMAANMIGVNKQIIVVQLGPFAIAMINPKIIDHHGTYE
TKEGCLSLPGERPTSRYHQITVKYKDQHFKPQQQRFNDFTAQIIQHELDHCAGKLI

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=125, Percent_Identity=27.2, Blast_Score=66, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 15048; Mature: 15048

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRPIVHDPAALSVPADLATPADTQVLTDLLDTLAAHTDNCVGMAANMIGVNKQIIVVQL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEE
GPFAIAMINPKIIDHHGTYETKEGCLSLPGERPTSRYHQITVKYKDQHFKPQQQRFNDFT
CCEEEEEECCEEECCCCCCCCCCCCEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
AQIIQHELDHCAGKLI
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIRPIVHDPAALSVPADLATPADTQVLTDLLDTLAAHTDNCVGMAANMIGVNKQIIVVQL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEEE
GPFAIAMINPKIIDHHGTYETKEGCLSLPGERPTSRYHQITVKYKDQHFKPQQQRFNDFT
CCEEEEEECCEEECCCCCCCCCCCCEECCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHH
AQIIQHELDHCAGKLI
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9572951 [H]