Click here to switch to the map view.

The map label for this gene is narI [H]

Identifier: 28378216

GI number: 28378216

Start: 1373935

End: 1374624

Strand: Direct

Name: narI [H]

Synonym: lp_1500

Alternate gene names: 28378216

Gene position: 1373935-1374624 (Clockwise)

Preceding gene: 28378215

Following gene: 28378217

Centisome position: 41.53

GC content: 46.81

Gene sequence:

>690_bases
ATGAAAGATTTCTTTTCCGTAATACTGTGGGTGATCTACCCGTATGCCATGTTATTATCGTTCTTCGTGGGGACGTTTGT
GCGGCTGAAATTTTATCCGGCTAGCGTCACGGCAGTTTCCAGTGAAATGCTGGAGAAGAAAAAACTGATGATTGGGGCCA
TCACTTTCCACGTTGGGATCATTTTAGCCTTTTTTGGGCATATCTTAGGGATTCTGATTCCGAAAGCTTTTACGGACTTT
TTGGGAATTTCAGATGAGATGTACCATATGTTCGGTTCCTTAATGATGGGGACTGGGGCCGGAGTTTTGGCCCTTGCCGG
GATGATTATCCTGACGTACCGGCGGTTTACCAATGTACGGGTGTTTGTCACCAGTTCTTGGAGCGATTTAATGGTCAACG
TGGCATTGTTGATTACGATCATTTTAGGATTGGCTTCAACACTATCCGGTCCTTTACATCCGGCGTTTGACTACCGGACA
ACACTCTCAGTGTGGGCGCGCTCATTGTTCTATCTCCAACCAAAGTGGTGGTTGATGGCTACAGTGCCGTGGATTTACAA
GACGCATGTTATTTGCGGCTTAGCAATTTTCGGCTTTTTCCCATACACGCGGCTGATTCACGCGATTGCAATTCCTTACC
AGTACTTCTATCGGCGTTACATCGTTTATCGGCGGCGGCCACGAGTTTAG

Upstream 100 bases:

>100_bases
TACAAAATGCAGCGGCCGAATTGGATGATCCATACTTCCGGTTGTTACAAGTCGTACGGGCGGAATTACGGACGTGTGTG
GAAACGGGGGTCGCGGCATC

Downstream 100 bases:

>100_bases
TGAATCGTTGCGAACTTTAAGCAAAAATGGTTTTTACGGCACGGATGGGTGCGGTAAAGGCCATTTTTTAGTTGCGCCGA
TTTGTCCTGGTCAAGCTGCG

Product: nitrate reductase, gamma chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKDFFSVILWVIYPYAMLLSFFVGTFVRLKFYPASVTAVSSEMLEKKKLMIGAITFHVGIILAFFGHILGILIPKAFTDF
LGISDEMYHMFGSLMMGTGAGVLALAGMIILTYRRFTNVRVFVTSSWSDLMVNVALLITIILGLASTLSGPLHPAFDYRT
TLSVWARSLFYLQPKWWLMATVPWIYKTHVICGLAIFGFFPYTRLIHAIAIPYQYFYRRYIVYRRRPRV

Sequences:

>Translated_229_residues
MKDFFSVILWVIYPYAMLLSFFVGTFVRLKFYPASVTAVSSEMLEKKKLMIGAITFHVGIILAFFGHILGILIPKAFTDF
LGISDEMYHMFGSLMMGTGAGVLALAGMIILTYRRFTNVRVFVTSSWSDLMVNVALLITIILGLASTLSGPLHPAFDYRT
TLSVWARSLFYLQPKWWLMATVPWIYKTHVICGLAIFGFFPYTRLIHAIAIPYQYFYRRYIVYRRRPRV
>Mature_229_residues
MKDFFSVILWVIYPYAMLLSFFVGTFVRLKFYPASVTAVSSEMLEKKKLMIGAITFHVGIILAFFGHILGILIPKAFTDF
LGISDEMYHMFGSLMMGTGAGVLALAGMIILTYRRFTNVRVFVTSSWSDLMVNVALLITIILGLASTLSGPLHPAFDYRT
TLSVWARSLFYLQPKWWLMATVPWIYKTHVICGLAIFGFFPYTRLIHAIAIPYQYFYRRYIVYRRRPRV

Specific function: This Is A Second Nitrate Reductase Enzyme Which Can Substitute For The Nra Enzyme And Allows E.Coli To Use Nitrate As An Electron Acceptor During Anaerobic Growth. The Gamma Chain Is A Membrane-Embedded Heme-Iron Unit Resembling Cytochrome B, Which Trans

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=228, Percent_Identity=33.3333333333333, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1787480, Length=227, Percent_Identity=29.9559471365639, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 26336; Mature: 26336

Theoretical pI: Translated: 10.34; Mature: 10.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDFFSVILWVIYPYAMLLSFFVGTFVRLKFYPASVTAVSSEMLEKKKLMIGAITFHVGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILAFFGHILGILIPKAFTDFLGISDEMYHMFGSLMMGTGAGVLALAGMIILTYRRFTNVR
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEE
VFVTSSWSDLMVNVALLITIILGLASTLSGPLHPAFDYRTTLSVWARSLFYLQPKWWLMA
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
TVPWIYKTHVICGLAIFGFFPYTRLIHAIAIPYQYFYRRYIVYRRRPRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKDFFSVILWVIYPYAMLLSFFVGTFVRLKFYPASVTAVSSEMLEKKKLMIGAITFHVGI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
ILAFFGHILGILIPKAFTDFLGISDEMYHMFGSLMMGTGAGVLALAGMIILTYRRFTNVR
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEE
VFVTSSWSDLMVNVALLITIILGLASTLSGPLHPAFDYRTTLSVWARSLFYLQPKWWLMA
EEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHH
TVPWIYKTHVICGLAIFGFFPYTRLIHAIAIPYQYFYRRYIVYRRRPRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8846791; 9353933; 9384377; 7557333 [H]