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The map label for this gene is narK [H]

Identifier: 28378197

GI number: 28378197

Start: 1355844

End: 1357034

Strand: Direct

Name: narK [H]

Synonym: lp_1481

Alternate gene names: 28378197

Gene position: 1355844-1357034 (Clockwise)

Preceding gene: 28378196

Following gene: 28378198

Centisome position: 40.98

GC content: 47.86

Gene sequence:

>1191_bases
ATGACTGTTGAAGATGCTCAAACAAGTACGCGTTGGCGGGCCTATTTAGCGTTAGGCTTGGCAACGGTGGCGATGATCGT
TTGCTTTATGTCATGGTCAAACTTTGCACCATTAGCGGGAGAGCTCGCAATCAAGTTTCACTTGAGTGTGTCGCAACGGA
CGCTGCTACTAGCAACGCCAGTACTACTCGGGTCAATCATGCGAATTCCAGTCGGAATTTTGAGTGATCGGTATGGTGGT
AAAAAGGTTTATCTGATCCTGATGGCCTTCATTTTGATCCCACTGTTGATGATTACGAAGGTACACACGTACGGTATGTT
GTTAGTCGCAGCGTTACTCGTTGGGATGGCCGGAACGTCTTTTGCTGTCGGGGTTTCGTATGCCTCAGTCTGGTTTCCGC
CTGAAAAGCAAGGACTAGCATTAGGAATCGTCAGCATGGGGAACATGGGGAATGCGGTCGCTGCGTTGACGTTGCCATCC
ATATCGACCTCGTATGGGTTTAACATGGTTTACTATTTCCTGATGGTATTAACGGTCATTTTGGCTGTCCTGTTCGCCAT
CTTTTGTCGCGAAATGCCGGTTGATAAGACGAAAACTGTGACGGGCGCATTGGCTGTCGCAAAGGAAAGTAGTACTTGGT
ACTTGTCATTATTTTATTTTCTAACGTTTGGTCTGTTTGTCTCATTCACTAATTTGACACCATTGTTCTTGGAAGATATT
TTCCGTGTGCCACAAGTAACAGCAGGCTTATACGCTGCCTTATTTGCTGGCCTTTGTACCGTTGTTCGACCAATTGGGGG
AGCGGCGGCCGATAAGTGGCGACCGATGCAGATGTTACAGTGGATCTTTATCGGCATTACCGTCTTTGCCGTGATTATTA
CGCTGACCTTTAGCAACCAGTCGCTGTTTATCTTTGGTATTGTTGGTGCCGGATTGATTGCCGGCTTCGGTAACGGGGTC
ATCTTTAAGATGGTGCCGTATGTATTACAAGGAAATACTGGTGCGGTCACCGGTTTTGTCGGCGCTTTGGGTGGCCTGGG
CGGTTTCTTCCCACCACTATTAGTGGGGTGGATTCATACTTGGACGGGAAGTTATCGGCTTGGTATCGTGCTCTTAGCCC
TCACGGGATTAGTTTGTTGGTATGCCCTTTGGCGCCGGTTTATTCATGGTGACGTCCACATTGTTAAATAA

Upstream 100 bases:

>100_bases
AATAAGCCGCTCAATCATGAAATGGCCATGTCCGAAACTGACCGCATCATTGATAAAGCCCCAACGTGGCGGCATATGAG
TCAGATTGGAGGATAAGAAA

Downstream 100 bases:

>100_bases
TTGTAATCGATTACAATTTAACGTTAATTATTAGTGATTGGCATAAAATGCCTTATAAAAATGGTATAATTAAACGAATA
ATAGTTAGAAGGAGTGTGGC

Product: nitrite extrusion protein

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MTVEDAQTSTRWRAYLALGLATVAMIVCFMSWSNFAPLAGELAIKFHLSVSQRTLLLATPVLLGSIMRIPVGILSDRYGG
KKVYLILMAFILIPLLMITKVHTYGMLLVAALLVGMAGTSFAVGVSYASVWFPPEKQGLALGIVSMGNMGNAVAALTLPS
ISTSYGFNMVYYFLMVLTVILAVLFAIFCREMPVDKTKTVTGALAVAKESSTWYLSLFYFLTFGLFVSFTNLTPLFLEDI
FRVPQVTAGLYAALFAGLCTVVRPIGGAAADKWRPMQMLQWIFIGITVFAVIITLTFSNQSLFIFGIVGAGLIAGFGNGV
IFKMVPYVLQGNTGAVTGFVGALGGLGGFFPPLLVGWIHTWTGSYRLGIVLLALTGLVCWYALWRRFIHGDVHIVK

Sequences:

>Translated_396_residues
MTVEDAQTSTRWRAYLALGLATVAMIVCFMSWSNFAPLAGELAIKFHLSVSQRTLLLATPVLLGSIMRIPVGILSDRYGG
KKVYLILMAFILIPLLMITKVHTYGMLLVAALLVGMAGTSFAVGVSYASVWFPPEKQGLALGIVSMGNMGNAVAALTLPS
ISTSYGFNMVYYFLMVLTVILAVLFAIFCREMPVDKTKTVTGALAVAKESSTWYLSLFYFLTFGLFVSFTNLTPLFLEDI
FRVPQVTAGLYAALFAGLCTVVRPIGGAAADKWRPMQMLQWIFIGITVFAVIITLTFSNQSLFIFGIVGAGLIAGFGNGV
IFKMVPYVLQGNTGAVTGFVGALGGLGGFFPPLLVGWIHTWTGSYRLGIVLLALTGLVCWYALWRRFIHGDVHIVK
>Mature_395_residues
TVEDAQTSTRWRAYLALGLATVAMIVCFMSWSNFAPLAGELAIKFHLSVSQRTLLLATPVLLGSIMRIPVGILSDRYGGK
KVYLILMAFILIPLLMITKVHTYGMLLVAALLVGMAGTSFAVGVSYASVWFPPEKQGLALGIVSMGNMGNAVAALTLPSI
STSYGFNMVYYFLMVLTVILAVLFAIFCREMPVDKTKTVTGALAVAKESSTWYLSLFYFLTFGLFVSFTNLTPLFLEDIF
RVPQVTAGLYAALFAGLCTVVRPIGGAAADKWRPMQMLQWIFIGITVFAVIITLTFSNQSLFIFGIVGAGLIAGFGNGVI
FKMVPYVLQGNTGAVTGFVGALGGLGGFFPPLLVGWIHTWTGSYRLGIVLLALTGLVCWYALWRRFIHGDVHIVK

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=393, Percent_Identity=23.6641221374046, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 42933; Mature: 42802

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVEDAQTSTRWRAYLALGLATVAMIVCFMSWSNFAPLAGELAIKFHLSVSQRTLLLATP
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHH
VLLGSIMRIPVGILSDRYGGKKVYLILMAFILIPLLMITKVHTYGMLLVAALLVGMAGTS
HHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FAVGVSYASVWFPPEKQGLALGIVSMGNMGNAVAALTLPSISTSYGFNMVYYFLMVLTVI
HHHHHHHHHEECCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
LAVLFAIFCREMPVDKTKTVTGALAVAKESSTWYLSLFYFLTFGLFVSFTNLTPLFLEDI
HHHHHHHHHHCCCCCCHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FRVPQVTAGLYAALFAGLCTVVRPIGGAAADKWRPMQMLQWIFIGITVFAVIITLTFSNQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCC
SLFIFGIVGAGLIAGFGNGVIFKMVPYVLQGNTGAVTGFVGALGGLGGFFPPLLVGWIHT
CEEEEEEHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
WTGSYRLGIVLLALTGLVCWYALWRRFIHGDVHIVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC
>Mature Secondary Structure 
TVEDAQTSTRWRAYLALGLATVAMIVCFMSWSNFAPLAGELAIKFHLSVSQRTLLLATP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCHHHHHHH
VLLGSIMRIPVGILSDRYGGKKVYLILMAFILIPLLMITKVHTYGMLLVAALLVGMAGTS
HHHHHHHHCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FAVGVSYASVWFPPEKQGLALGIVSMGNMGNAVAALTLPSISTSYGFNMVYYFLMVLTVI
HHHHHHHHHEECCCCCCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
LAVLFAIFCREMPVDKTKTVTGALAVAKESSTWYLSLFYFLTFGLFVSFTNLTPLFLEDI
HHHHHHHHHHCCCCCCHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHH
FRVPQVTAGLYAALFAGLCTVVRPIGGAAADKWRPMQMLQWIFIGITVFAVIITLTFSNQ
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEECCC
SLFIFGIVGAGLIAGFGNGVIFKMVPYVLQGNTGAVTGFVGALGGLGGFFPPLLVGWIHT
CEEEEEEHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
WTGSYRLGIVLLALTGLVCWYALWRRFIHGDVHIVK
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]