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The map label for this gene is moaA

Identifier: 28378196

GI number: 28378196

Start: 1354841

End: 1355839

Strand: Direct

Name: moaA

Synonym: lp_1480

Alternate gene names: 28378196

Gene position: 1354841-1355839 (Clockwise)

Preceding gene: 28378195

Following gene: 28378197

Centisome position: 40.95

GC content: 43.04

Gene sequence:

>999_bases
ATGGAAAAATTATATGACAGTTATGATCGCTTGCATGATTACGTGCGGTTATCGATTACGGATCGTTGTAATTTGCGGTG
TGTATATTGTATGCCGAAAGAGGGGCTACCGTTTTTCCCAACGGATCGGGTCCTGTCACAGGATGAAATTGTCCAATTGA
TCGAGAATTTTGCGGCCATGGGAGTGTCTAAGGTCCGAATCACTGGTGGCGAACCACTGCTACGGACCGATGTGGTCGAA
ATTGTTCGGCGTATCAAGGCGGTCGACGGGATTAACGATGTTTCAATCACAACTAATGGGCTATTTTTGGCGAAATTAGC
TAAGCCACTGAAAGAAGCCGGATTGGACCGGTTGAATATTAGTCTGGATACGTTTAAAGCGGATCGGTACAAAAAAATTA
CGCGGGGCGGTAATATTCAACAGGTTTTAGATGGAATCGCCGTGGCTAGTAAGTTACATTTCAAAAAGATCAAGTTGAAC
ATTGTTTTGATCAAGGGGCAAAATGATGACGAAGTCCTAGATTTCTTACATTACACCAAGGACCACGATGTTAATGCGCG
CTTCATTGAGTTTATGCCGATTGGGAATTCATTAAAGACCTGGCAAAAAGAATACGTGGGTTTAAAAAACGTCTTTGATA
CTTGTAAAGACAACGGCTTAGCCTACCATCCAATCGTTTTGCGTGGTAACGGACCGTCTGATAATTATCAAATTGAAGGT
TATGAGGGAAGTTTCGGTCTTATTCATCCAATCAGTTCTAAGTTTTGTGAAAACTGTAATCGATTACGAATTACTGCTGA
CGGGTACGTCAAAGCATGCTTGTATTGGAACGAAGAAATCGATATCCGGTCGGCCATTGGTGATCCGGTGGCATTCCGCA
AACTTATTCAAAAGGCGTTAGATAATAAGCCGCTCAATCATGAAATGGCCATGTCCGAAACTGACCGCATCATTGATAAA
GCCCCAACGTGGCGGCATATGAGTCAGATTGGAGGATAA

Upstream 100 bases:

>100_bases
CAAGTTTTACAATCGACTGATGAGATTGCACTAATTCCGCCGGTCAGTGGGGGTTAAATAGCGGCGTTAGATCTAATGAT
GAAGGAGGAGCGCGAATAGT

Downstream 100 bases:

>100_bases
GAAAATGACTGTTGAAGATGCTCAAACAAGTACGCGTTGGCGGGCCTATTTAGCGTTAGGCTTGGCAACGGTGGCGATGA
TCGTTTGCTTTATGTCATGG

Product: molybdenum cofactor biosynthesis protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MEKLYDSYDRLHDYVRLSITDRCNLRCVYCMPKEGLPFFPTDRVLSQDEIVQLIENFAAMGVSKVRITGGEPLLRTDVVE
IVRRIKAVDGINDVSITTNGLFLAKLAKPLKEAGLDRLNISLDTFKADRYKKITRGGNIQQVLDGIAVASKLHFKKIKLN
IVLIKGQNDDEVLDFLHYTKDHDVNARFIEFMPIGNSLKTWQKEYVGLKNVFDTCKDNGLAYHPIVLRGNGPSDNYQIEG
YEGSFGLIHPISSKFCENCNRLRITADGYVKACLYWNEEIDIRSAIGDPVAFRKLIQKALDNKPLNHEMAMSETDRIIDK
APTWRHMSQIGG

Sequences:

>Translated_332_residues
MEKLYDSYDRLHDYVRLSITDRCNLRCVYCMPKEGLPFFPTDRVLSQDEIVQLIENFAAMGVSKVRITGGEPLLRTDVVE
IVRRIKAVDGINDVSITTNGLFLAKLAKPLKEAGLDRLNISLDTFKADRYKKITRGGNIQQVLDGIAVASKLHFKKIKLN
IVLIKGQNDDEVLDFLHYTKDHDVNARFIEFMPIGNSLKTWQKEYVGLKNVFDTCKDNGLAYHPIVLRGNGPSDNYQIEG
YEGSFGLIHPISSKFCENCNRLRITADGYVKACLYWNEEIDIRSAIGDPVAFRKLIQKALDNKPLNHEMAMSETDRIIDK
APTWRHMSQIGG
>Mature_332_residues
MEKLYDSYDRLHDYVRLSITDRCNLRCVYCMPKEGLPFFPTDRVLSQDEIVQLIENFAAMGVSKVRITGGEPLLRTDVVE
IVRRIKAVDGINDVSITTNGLFLAKLAKPLKEAGLDRLNISLDTFKADRYKKITRGGNIQQVLDGIAVASKLHFKKIKLN
IVLIKGQNDDEVLDFLHYTKDHDVNARFIEFMPIGNSLKTWQKEYVGLKNVFDTCKDNGLAYHPIVLRGNGPSDNYQIEG
YEGSFGLIHPISSKFCENCNRLRITADGYVKACLYWNEEIDIRSAIGDPVAFRKLIQKALDNKPLNHEMAMSETDRIIDK
APTWRHMSQIGG

Specific function: Together with moaC, is involved in the conversion of a guanosine derivative (5'-GTP) into molybdopterin precursor Z

COG id: COG2896

COG function: function code H; Molybdenum cofactor biosynthesis enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. MoaA family

Homologues:

Organism=Homo sapiens, GI116734668, Length=282, Percent_Identity=40.7801418439716, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI22027536, Length=282, Percent_Identity=40.7801418439716, Blast_Score=239, Evalue=2e-63,
Organism=Escherichia coli, GI1786999, Length=316, Percent_Identity=33.5443037974684, Blast_Score=190, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI32566331, Length=314, Percent_Identity=42.9936305732484, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI32566329, Length=312, Percent_Identity=43.2692307692308, Blast_Score=264, Evalue=6e-71,
Organism=Caenorhabditis elegans, GI17561210, Length=312, Percent_Identity=42.9487179487179, Blast_Score=261, Evalue=3e-70,
Organism=Drosophila melanogaster, GI28574490, Length=331, Percent_Identity=40.785498489426, Blast_Score=245, Evalue=3e-65,
Organism=Drosophila melanogaster, GI28574488, Length=289, Percent_Identity=43.598615916955, Blast_Score=244, Evalue=6e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MOAA_LACPL (Q88WY1)

Other databases:

- EMBL:   AL935256
- RefSeq:   NP_785088.1
- ProteinModelPortal:   Q88WY1
- SMR:   Q88WY1
- GeneID:   1062330
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1480
- NMPDR:   fig|220668.1.peg.1222
- HOGENOM:   HBG529885
- OMA:   IGEASGW
- ProtClustDB:   PRK00164
- BioCyc:   LPLA220668:LP_1480-MONOMER
- HAMAP:   MF_01225_B
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR013483
- InterPro:   IPR000385
- InterPro:   IPR010505
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00729
- TIGRFAMs:   TIGR02666

Pfam domain/function: PF06463 Mob_synth_C; PF04055 Radical_SAM

EC number: NA

Molecular weight: Translated: 37729; Mature: 37729

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: BINDING 16-16 BINDING 29-29 BINDING 66-66 BINDING 70-70 BINDING 97-97 BINDING 121-121 BINDING 158-158 BINDING 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLYDSYDRLHDYVRLSITDRCNLRCVYCMPKEGLPFFPTDRVLSQDEIVQLIENFAAM
CCHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCCCCCCCCHHCCHHHHHHHHHHHHHC
GVSKVRITGGEPLLRTDVVEIVRRIKAVDGINDVSITTNGLFLAKLAKPLKEAGLDRLNI
CCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEE
SLDTFKADRYKKITRGGNIQQVLDGIAVASKLHFKKIKLNIVLIKGQNDDEVLDFLHYTK
EEEHHCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCHHHHHHHHHCC
DHDVNARFIEFMPIGNSLKTWQKEYVGLKNVFDTCKDNGLAYHPIVLRGNGPSDNYQIEG
CCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHCCCCEEECCEEEECCCCCCCEEEEE
YEGSFGLIHPISSKFCENCNRLRITADGYVKACLYWNEEIDIRSAIGDPVAFRKLIQKAL
ECCCCCEECCHHHHHHCCCCEEEEEECCEEEEEEEECCCCCHHHHCCCHHHHHHHHHHHH
DNKPLNHEMAMSETDRIIDKAPTWRHMSQIGG
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure
MEKLYDSYDRLHDYVRLSITDRCNLRCVYCMPKEGLPFFPTDRVLSQDEIVQLIENFAAM
CCHHHHHHHHHHHEEEEEEECCCCEEEEEEECCCCCCCCCCCHHCCHHHHHHHHHHHHHC
GVSKVRITGGEPLLRTDVVEIVRRIKAVDGINDVSITTNGLFLAKLAKPLKEAGLDRLNI
CCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCEEEE
SLDTFKADRYKKITRGGNIQQVLDGIAVASKLHFKKIKLNIVLIKGQNDDEVLDFLHYTK
EEEHHCCHHHHHHHCCCCHHHHHHHHHHHHHHEEEEEEEEEEEEECCCCHHHHHHHHHCC
DHDVNARFIEFMPIGNSLKTWQKEYVGLKNVFDTCKDNGLAYHPIVLRGNGPSDNYQIEG
CCCCCCEEEEEEECCCHHHHHHHHHCCHHHHHHHHCCCCEEECCEEEECCCCCCCEEEEE
YEGSFGLIHPISSKFCENCNRLRITADGYVKACLYWNEEIDIRSAIGDPVAFRKLIQKAL
ECCCCCEECCHHHHHHCCCCEEEEEECCEEEEEEEECCCCCHHHHCCCHHHHHHHHHHHH
DNKPLNHEMAMSETDRIIDKAPTWRHMSQIGG
CCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566