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The map label for this gene is rsuA [H]

Identifier: 28378068

GI number: 28378068

Start: 1214810

End: 1215550

Strand: Direct

Name: rsuA [H]

Synonym: lp_1319

Alternate gene names: 28378068

Gene position: 1214810-1215550 (Clockwise)

Preceding gene: 28378067

Following gene: 28378070

Centisome position: 36.72

GC content: 47.64

Gene sequence:

>741_bases
ATGCGAGTAGATAAATTTTTACATGCGATGCGAATTGGCACGCGGACTCAAGTACGTCGTCTGATTAAAGATGGTCATAT
CACTGCCAATGGTGAGCCAGTAATCTCTGGCAAACAACAGATTAACCCTGGCAGTGATACTGTCTTTTTAGATGACGTGC
AGGTCCGTTACCAACAATACTTCTATTATGTGATGAATAAGCCAGTCGGGGTCATCACGGCAACGACGGATTCCGCTGCG
AAAACGGTGATGGACCTGTTCAATACGACGGATTTTCGTGACGATCTATTTCCCGTGGGTCGTCTCGATAAAGATACTGA
GGGAATCTTGGTCATTACGAACGATGGGGCCCTATCGCACGACCTATTGTCGCCGAACCACCATGTTACGAAGGTTTATG
AGGCTGAAGTAACGGGGGAAATCACTGCAGCTCAATTGGCTAATTTTAATGATGGGATCACGTTAAAGGACGGGACTGAA
TTGCAACCGGCTCAGGCTGAGATTGTTCAATTCCATGAAATCGAGAACTTTACCACGATTCGAATCATGATCCATGAAGG
CAAGTATCACCAAGTTAAGCGGATGGTCGGAACGTTAGGTGAACGGGTCGTCGGGTTACGGCGAATCAAGTTTGGTTCGT
TAACGCTACCCGTTGGCTTATTAGCGGGGAACTATCGTGCGCTGACTGATGACGAACTAACAGCGTTGAAGGCGGATGCG
AACCAACCCGTAGGCGAATAG

Upstream 100 bases:

>100_bases
GTTTACGTTTACTTAGCACTCCGTTCACGGATGGCTGATTTGATGATTGGAACGAAAGCTGCTGGGTTACGACGGAAATT
ACGGATTAAATAGGTGAATT

Downstream 100 bases:

>100_bases
TGCGTGTTACGAATTCGGCCACTCTGGTTTAAACTAAATCATTAAGCCCCAACTAACTATGAATTCAAGCATGGTTAGTT
GAGGCTTTTTTAGCATAATG

Product: pseudouridylate synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRVDKFLHAMRIGTRTQVRRLIKDGHITANGEPVISGKQQINPGSDTVFLDDVQVRYQQYFYYVMNKPVGVITATTDSAA
KTVMDLFNTTDFRDDLFPVGRLDKDTEGILVITNDGALSHDLLSPNHHVTKVYEAEVTGEITAAQLANFNDGITLKDGTE
LQPAQAEIVQFHEIENFTTIRIMIHEGKYHQVKRMVGTLGERVVGLRRIKFGSLTLPVGLLAGNYRALTDDELTALKADA
NQPVGE

Sequences:

>Translated_246_residues
MRVDKFLHAMRIGTRTQVRRLIKDGHITANGEPVISGKQQINPGSDTVFLDDVQVRYQQYFYYVMNKPVGVITATTDSAA
KTVMDLFNTTDFRDDLFPVGRLDKDTEGILVITNDGALSHDLLSPNHHVTKVYEAEVTGEITAAQLANFNDGITLKDGTE
LQPAQAEIVQFHEIENFTTIRIMIHEGKYHQVKRMVGTLGERVVGLRRIKFGSLTLPVGLLAGNYRALTDDELTALKADA
NQPVGE
>Mature_246_residues
MRVDKFLHAMRIGTRTQVRRLIKDGHITANGEPVISGKQQINPGSDTVFLDDVQVRYQQYFYYVMNKPVGVITATTDSAA
KTVMDLFNTTDFRDDLFPVGRLDKDTEGILVITNDGALSHDLLSPNHHVTKVYEAEVTGEITAAQLANFNDGITLKDGTE
LQPAQAEIVQFHEIENFTTIRIMIHEGKYHQVKRMVGTLGERVVGLRRIKFGSLTLPVGLLAGNYRALTDDELTALKADA
NQPVGE

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=238, Percent_Identity=33.1932773109244, Blast_Score=127, Evalue=6e-31,
Organism=Escherichia coli, GI1790453, Length=239, Percent_Identity=28.0334728033473, Blast_Score=90, Evalue=1e-19,
Organism=Escherichia coli, GI87081838, Length=180, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787524, Length=238, Percent_Identity=27.7310924369748, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 27354; Mature: 27354

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVDKFLHAMRIGTRTQVRRLIKDGHITANGEPVISGKQQINPGSDTVFLDDVQVRYQQY
CCHHHHHHHHHCCCHHHHHHHHHCCCEECCCCEEECCCCCCCCCCCEEEECHHHHHHHHH
FYYVMNKPVGVITATTDSAAKTVMDLFNTTDFRDDLFPVGRLDKDTEGILVITNDGALSH
EEEEECCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
DLLSPNHHVTKVYEAEVTGEITAAQLANFNDGITLKDGTELQPAQAEIVQFHEIENFTTI
CCCCCCCCEEEEEEEECCCCCHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEE
RIMIHEGKYHQVKRMVGTLGERVVGLRRIKFGSLTLPVGLLAGNYRALTDDELTALKADA
EEEEECCCHHHHHHHHHHHHHHHHHHHEEEECCEECCHHEEECCCEEECCCCCEEEECCC
NQPVGE
CCCCCC
>Mature Secondary Structure
MRVDKFLHAMRIGTRTQVRRLIKDGHITANGEPVISGKQQINPGSDTVFLDDVQVRYQQY
CCHHHHHHHHHCCCHHHHHHHHHCCCEECCCCEEECCCCCCCCCCCEEEECHHHHHHHHH
FYYVMNKPVGVITATTDSAAKTVMDLFNTTDFRDDLFPVGRLDKDTEGILVITNDGALSH
EEEEECCCEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCC
DLLSPNHHVTKVYEAEVTGEITAAQLANFNDGITLKDGTELQPAQAEIVQFHEIENFTTI
CCCCCCCCEEEEEEEECCCCCHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHCCCEEEE
RIMIHEGKYHQVKRMVGTLGERVVGLRRIKFGSLTLPVGLLAGNYRALTDDELTALKADA
EEEEECCCHHHHHHHHHHHHHHHHHHHEEEECCEECCHHEEECCCEEECCCCCEEEECCC
NQPVGE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]