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The map label for this gene is glpF4 [H]

Identifier: 28377949

GI number: 28377949

Start: 1072312

End: 1073028

Strand: Direct

Name: glpF4 [H]

Synonym: lp_1175

Alternate gene names: 28377949

Gene position: 1072312-1073028 (Clockwise)

Preceding gene: 28377948

Following gene: 28377950

Centisome position: 32.41

GC content: 48.68

Gene sequence:

>717_bases
GTGATTCATCAGCTTTTGGCAGAGTTCATGGGAACCGCCTTGATGATTATTTTTGGTGTCGGTGTACATTGTAGTGAGGT
TTTAAAGGGGACCAAGTATCGCGGTTCCGGCCATATCTTCGCAATTACAACATGGGGTTTTGGGATTACAATTGCCCTCT
TCATTTTCGGCAATGTCTGCATTAACCCGGCCATGGTATTAGCCCAATGTATTTTAGGGAACCTTTCATGGTCATTGTTT
ATCCCGTATTCAGTTGCAGAAGTTTTGGGTGGGGTCGTTGGTGCCGTGATCGTCTGGATCATGTACGCGGACCATTTTGC
CGCTTCTGCTGATGAGATTTCCCCAATTACGATTCGGAACTTATTCTCAACCGCACCCGCTGTGCGGAACTTACCACGGA
ACTTCTTCGTCGAATTCTTCGATACATTTATTTTTATTTCTGGGATTTTAGCTATTTCTGAAGTGAAGACGCCGGGAATC
GTGCCGATTGGTGTTGGGCTCTTAGTCTGGGCCATTGGGATGGGGCTAGGTGGCCCAACCGGATTCGCCATGAACCTTGC
ACGGGACATGGGCCCACGGATCGCCCACGCCATCTTACCAATTAAGAACAAGGCTGACAGCGACTGGCAATACGGGATCA
TTGTTCCTGGGATTGCACCTTTCGTTGGGGCTGCTTGTGCAGCGTTGTTTATGCATGGGTTCTTTGGGATTGGGTAG

Upstream 100 bases:

>100_bases
TAATGGTGTTCGTGTGAAAAAAGTGCCGGTGCTTTCTGAAAGTAAGTGGCGTCTATTTCAAAATAAACAGGCGTAACAAC
GCCACAGGAGGAGAAAGATA

Downstream 100 bases:

>100_bases
ATAATAAGGCATAAATTATAGGAAAAGCGATCATCTATGGAGGTGGTCGCTTTTTAACAGTGTTAAAACGATAATCTTAT
AATTAGCCTTTTACATTCAG

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MIHQLLAEFMGTALMIIFGVGVHCSEVLKGTKYRGSGHIFAITTWGFGITIALFIFGNVCINPAMVLAQCILGNLSWSLF
IPYSVAEVLGGVVGAVIVWIMYADHFAASADEISPITIRNLFSTAPAVRNLPRNFFVEFFDTFIFISGILAISEVKTPGI
VPIGVGLLVWAIGMGLGGPTGFAMNLARDMGPRIAHAILPIKNKADSDWQYGIIVPGIAPFVGAACAALFMHGFFGIG

Sequences:

>Translated_238_residues
MIHQLLAEFMGTALMIIFGVGVHCSEVLKGTKYRGSGHIFAITTWGFGITIALFIFGNVCINPAMVLAQCILGNLSWSLF
IPYSVAEVLGGVVGAVIVWIMYADHFAASADEISPITIRNLFSTAPAVRNLPRNFFVEFFDTFIFISGILAISEVKTPGI
VPIGVGLLVWAIGMGLGGPTGFAMNLARDMGPRIAHAILPIKNKADSDWQYGIIVPGIAPFVGAACAALFMHGFFGIG
>Mature_238_residues
MIHQLLAEFMGTALMIIFGVGVHCSEVLKGTKYRGSGHIFAITTWGFGITIALFIFGNVCINPAMVLAQCILGNLSWSLF
IPYSVAEVLGGVVGAVIVWIMYADHFAASADEISPITIRNLFSTAPAVRNLPRNFFVEFFDTFIFISGILAISEVKTPGI
VPIGVGLLVWAIGMGLGGPTGFAMNLARDMGPRIAHAILPIKNKADSDWQYGIIVPGIAPFVGAACAALFMHGFFGIG

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI4826645, Length=237, Percent_Identity=32.4894514767932, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI22538420, Length=240, Percent_Identity=29.5833333333333, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4502187, Length=236, Percent_Identity=30.5084745762712, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI157266307, Length=245, Percent_Identity=29.7959183673469, Blast_Score=81, Evalue=9e-16,
Organism=Escherichia coli, GI1790362, Length=251, Percent_Identity=31.0756972111554, Blast_Score=85, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71992966, Length=208, Percent_Identity=30.2884615384615, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994009, Length=244, Percent_Identity=27.0491803278689, Blast_Score=70, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI17531431, Length=240, Percent_Identity=30.4166666666667, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17531429, Length=240, Percent_Identity=30.4166666666667, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6321054, Length=205, Percent_Identity=30.2439024390244, Blast_Score=79, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 25367; Mature: 25367

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIHQLLAEFMGTALMIIFGVGVHCSEVLKGTKYRGSGHIFAITTWGFGITIALFIFGNVC
CHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHH
INPAMVLAQCILGNLSWSLFIPYSVAEVLGGVVGAVIVWIMYADHFAASADEISPITIRN
CCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LFSTAPAVRNLPRNFFVEFFDTFIFISGILAISEVKTPGIVPIGVGLLVWAIGMGLGGPT
HHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCH
GFAMNLARDMGPRIAHAILPIKNKADSDWQYGIIVPGIAPFVGAACAALFMHGFFGIG
HHHHHHHHHHCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIHQLLAEFMGTALMIIFGVGVHCSEVLKGTKYRGSGHIFAITTWGFGITIALFIFGNVC
CHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCEEEEEECHHHHHHHHHHHHHHH
INPAMVLAQCILGNLSWSLFIPYSVAEVLGGVVGAVIVWIMYADHFAASADEISPITIRN
CCHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
LFSTAPAVRNLPRNFFVEFFDTFIFISGILAISEVKTPGIVPIGVGLLVWAIGMGLGGPT
HHHHCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCH
GFAMNLARDMGPRIAHAILPIKNKADSDWQYGIIVPGIAPFVGAACAALFMHGFFGIG
HHHHHHHHHHCHHHHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]