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The map label for this gene is dagK [H]

Identifier: 28377928

GI number: 28377928

Start: 1047405

End: 1048427

Strand: Direct

Name: dagK [H]

Synonym: lp_1150

Alternate gene names: 28377928

Gene position: 1047405-1048427 (Clockwise)

Preceding gene: 28377927

Following gene: 28377929

Centisome position: 31.66

GC content: 48.29

Gene sequence:

>1023_bases
ATGCGTAAACGGGCACGACTTATTTATAACCCGACTTCTGGACGTGAGGCATTGGCCCACGATTTAGTTGAAATTCTTGG
TATCTTTGAACAAGCTGGTTATGAGACCAGTGCGTTCGCAACGACGCCCGCCCCTAATTCGGCGGCTGATGAAGCGCGAC
GTTGTGCCAAGGACGGGTTTGAACTGATTGTAGCAGCTGGTGGTGATGGGACCATCAATCAAGTAGTAAACGGGTTAGCC
GGGTTAAAGCGGCGACCTAAGATGGCTATTATTCCGGCGGGTACGACCAACGATTATGCCCGGGGACTGCGGATTCCGCG
CAATGATCCGGTTGCGGCGGCCAAGGTAGTCCTAAAGAACCAGACGGTTAAGATGGATATCGGTCAGGCTGGTGAACAGT
ATTTCATTAACATTGCGGCTGGGGGCATGTTAACGGAGCTGACCTATGACGTACCATCACAATTGAAGTCGATTTTTGGG
TACGCGGCATACTTAGCCAAGGGTGCTGAGTTATTACCACGAGTAAAACCAGTTGAGATGGATTTGAAATATGATGGTGG
TCATTATCAGGGCAAGGCTTCGATGTTTTTCTTAGCGCTGACTAATTCAGTTGGTGGCTTTGAGCAGTTAGTGCCGGACG
CGTCATTAGATGATGGTAAATTTACCATGATTATTGTGAAGACGAGCAACCTTGCCAAAATGCTTCACTTAATGACATTA
GTGCTCAATGGTGGCAAGCATATCAATGATCCAAGCATTATTTATGTGAAGACGAGCAAGGTCGTCGCCAAACCAGCTGA
CAAGGATCGCTTAATGATCAACTTGGATGGGGAGTACGGTGGTGATGCACCGATGACGTTCCGGAATTTGAAGCAACATA
TCGAAATGTTTGCCGATACGGATCGGATCCCCGATGAGGCAATCACGGATACAGATCCAGAGTTGGAAGACGCTGAGCGA
CACTTTGTCTCAGCCGTTGATCAGTTGCCAGCTGCAACTGAGGACGACGAAAAAACAGAATAA

Upstream 100 bases:

>100_bases
GTTGGTCGATAAGGTGTTGAGCCTCGATTGATGCATGTCCAGCGTTGGGGTAGAGATGAGACGCTGAACGCTTGACCGTG
TGAGCGGAGGAATAGGAAAC

Downstream 100 bases:

>100_bases
CTTGCTTAAATTACCGTTTACCGATACAATGGTGGACGGTAATTTTTTTAAAAGTGTTACCGTCACAGGGACCTGATTGA
GCATTGCCTAACGATTTAAT

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MRKRARLIYNPTSGREALAHDLVEILGIFEQAGYETSAFATTPAPNSAADEARRCAKDGFELIVAAGGDGTINQVVNGLA
GLKRRPKMAIIPAGTTNDYARGLRIPRNDPVAAAKVVLKNQTVKMDIGQAGEQYFINIAAGGMLTELTYDVPSQLKSIFG
YAAYLAKGAELLPRVKPVEMDLKYDGGHYQGKASMFFLALTNSVGGFEQLVPDASLDDGKFTMIIVKTSNLAKMLHLMTL
VLNGGKHINDPSIIYVKTSKVVAKPADKDRLMINLDGEYGGDAPMTFRNLKQHIEMFADTDRIPDEAITDTDPELEDAER
HFVSAVDQLPAATEDDEKTE

Sequences:

>Translated_340_residues
MRKRARLIYNPTSGREALAHDLVEILGIFEQAGYETSAFATTPAPNSAADEARRCAKDGFELIVAAGGDGTINQVVNGLA
GLKRRPKMAIIPAGTTNDYARGLRIPRNDPVAAAKVVLKNQTVKMDIGQAGEQYFINIAAGGMLTELTYDVPSQLKSIFG
YAAYLAKGAELLPRVKPVEMDLKYDGGHYQGKASMFFLALTNSVGGFEQLVPDASLDDGKFTMIIVKTSNLAKMLHLMTL
VLNGGKHINDPSIIYVKTSKVVAKPADKDRLMINLDGEYGGDAPMTFRNLKQHIEMFADTDRIPDEAITDTDPELEDAER
HFVSAVDQLPAATEDDEKTE
>Mature_340_residues
MRKRARLIYNPTSGREALAHDLVEILGIFEQAGYETSAFATTPAPNSAADEARRCAKDGFELIVAAGGDGTINQVVNGLA
GLKRRPKMAIIPAGTTNDYARGLRIPRNDPVAAAKVVLKNQTVKMDIGQAGEQYFINIAAGGMLTELTYDVPSQLKSIFG
YAAYLAKGAELLPRVKPVEMDLKYDGGHYQGKASMFFLALTNSVGGFEQLVPDASLDDGKFTMIIVKTSNLAKMLHLMTL
VLNGGKHINDPSIIYVKTSKVVAKPADKDRLMINLDGEYGGDAPMTFRNLKQHIEMFADTDRIPDEAITDTDPELEDAER
HFVSAVDQLPAATEDDEKTE

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=252, Percent_Identity=30.5555555555556, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 36981; Mature: 36981

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKRARLIYNPTSGREALAHDLVEILGIFEQAGYETSAFATTPAPNSAADEARRCAKDGF
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCE
ELIVAAGGDGTINQVVNGLAGLKRRPKMAIIPAGTTNDYARGLRIPRNDPVAAAKVVLKN
EEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHCC
QTVKMDIGQAGEQYFINIAAGGMLTELTYDVPSQLKSIFGYAAYLAKGAELLPRVKPVEM
CEEEEECCCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEE
DLKYDGGHYQGKASMFFLALTNSVGGFEQLVPDASLDDGKFTMIIVKTSNLAKMLHLMTL
EEEECCCCCCCCCEEEEEEEECCCCCHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHH
VLNGGKHINDPSIIYVKTSKVVAKPADKDRLMINLDGEYGGDAPMTFRNLKQHIEMFADT
HHCCCCCCCCCEEEEEECCCEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCC
DRIPDEAITDTDPELEDAERHFVSAVDQLPAATEDDEKTE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MRKRARLIYNPTSGREALAHDLVEILGIFEQAGYETSAFATTPAPNSAADEARRCAKDGF
CCCCCEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHCCCE
ELIVAAGGDGTINQVVNGLAGLKRRPKMAIIPAGTTNDYARGLRIPRNDPVAAAKVVLKN
EEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHCCCCCCCCCHHHHHHHHCC
QTVKMDIGQAGEQYFINIAAGGMLTELTYDVPSQLKSIFGYAAYLAKGAELLPRVKPVEM
CEEEEECCCCCCEEEEEEECCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCEEE
DLKYDGGHYQGKASMFFLALTNSVGGFEQLVPDASLDDGKFTMIIVKTSNLAKMLHLMTL
EEEECCCCCCCCCEEEEEEEECCCCCHHHHCCCCCCCCCCEEEEEEECCHHHHHHHHHHH
VLNGGKHINDPSIIYVKTSKVVAKPADKDRLMINLDGEYGGDAPMTFRNLKQHIEMFADT
HHCCCCCCCCCEEEEEECCCEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCC
DRIPDEAITDTDPELEDAERHFVSAVDQLPAATEDDEKTE
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]