Click here to switch to the map view.

The map label for this gene is gatB

Identifier: 28377927

GI number: 28377927

Start: 1045876

End: 1047300

Strand: Direct

Name: gatB

Synonym: lp_1149

Alternate gene names: 28377927

Gene position: 1045876-1047300 (Clockwise)

Preceding gene: 28377926

Following gene: 28377928

Centisome position: 31.61

GC content: 44.84

Gene sequence:

>1425_bases
ATGAATTTTGTAACCACGATTGGTTTGGAAGTCCACGTTGAATTAAAAACGAACTCAAAAATCTTTAGTCCTTCACCCGT
GCAATTTGGTTCCGAACCAAATGCCAACACGAACGTGATCGACTGGGGGTATCCGGGGGTATTGCCAACGCCTAACAAGG
GCGTGGTCGAAGCTGGGATTAAAGCAGCAACGGCTTTACATGCTGAAATCGAACATCACACGTACTTTGACCGGAAGAAC
TATTTTTACCCGGATAACCCGAAGGCCTACCAAATTACGCAACATGAAAAACCAATTGCGCATGATGGTTGGATTGAAAT
CGAAGTTGATGGTAAAAAGAAAAAAATCGGTATCGAAGAGATGCATATTGAAGAAGATGCCGGGAAAAACACCCATGAAA
ACGACTATTCCTATGTCGATTTGAACCGGCAAGGGACGCCGCTAATTGAAATTGTTTCTAAGCCTGACATTGCCTCCCCA
GAAGAAGCTTATGCTTACTGTGAAGCGTTGCGGCAACGGATCCAATTTACGGGCGTTTCGGACGTTAAGATGGAAGAAGG
TTCAATGCGGGTCGATGTTAACATTTCAATTCGGCCGGCTGGTTCCGACAAGTATGGTGTGAAGACCGAAATGAAGAACT
TGAACTCCTTCAACTACGTGCGCAAGTCGTTAGAATATGAAGAACAACGCCAACAACAAGTTCTCATGGCGGGTGGCAAG
ATCCAACAAGAAACGCGGCGTTTTGATGAAACAACGGGCCAAACGATCTTGATGCGGGTCAAGGAAGGTAGCGATGATTA
CCGTTACTTCCCAGAACCTGATATTCCAGCAGTCGATATCGATGATGACTGGATTGCATCCGTTAAGAAGACCATTCCAG
AAATGCCTGGTTCGCGGCGTGAACGTTACATCAATGAATTTGGTTTAACTGCTTATGATGCGGGTGTGTTGACCCAAACT
AAGGAAATGTCTGACTTCTTTGATGCAACCGTTGCTGAAGGTGCGGATCCTAAATTAGCTTCAAACTACTTGCAAGGGGA
CGTTAACGCGTACTTGAACGAGCATAAAGTTGATTTGTTAGCGACTGACTTAACGCCAACTAACTTAGCTGGGATGATCA
AGATGATTTCAGATGAAACCATCTCAAGTAAAATTGCTAAGAAGGTCTTTAAGGCCATTATGGCGGGCTCAGAGCCTGTC
CAATGGGTCAACGACAAGGGCTTAGTCCAATTGTCTGATCCGGCGAAGTTACAACCAATCGTTGACGACGTATTGGATAA
TAATCAACAATCAATTGATGACTTTAAGAACGGGAAAGACCGGGCCGTTGGGTTCCTCGTGGGTCAAATCATGAAGAAGA
CACGTGGTCAAGCTAACCCGAAAGTCGTCAACCAACTATTAATGACATCATTAAAAGCACGGTAG

Upstream 100 bases:

>100_bases
GTAAGGCGTTTGATGAAAGCACAATGTATCGAGCGGGCTATGCCTTTGAACAAGCAACGCAAGTTTACAAGCAAACCCCG
AAAGTCGGAGGTGCTAACTA

Downstream 100 bases:

>100_bases
AAGCGTTGGTCGATAAGGTGTTGAGCCTCGATTGATGCATGTCCAGCGTTGGGGTAGAGATGAGACGCTGAACGCTTGAC
CGTGTGAGCGGAGGAATAGG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 474; Mature: 474

Protein sequence:

>474_residues
MNFVTTIGLEVHVELKTNSKIFSPSPVQFGSEPNANTNVIDWGYPGVLPTPNKGVVEAGIKAATALHAEIEHHTYFDRKN
YFYPDNPKAYQITQHEKPIAHDGWIEIEVDGKKKKIGIEEMHIEEDAGKNTHENDYSYVDLNRQGTPLIEIVSKPDIASP
EEAYAYCEALRQRIQFTGVSDVKMEEGSMRVDVNISIRPAGSDKYGVKTEMKNLNSFNYVRKSLEYEEQRQQQVLMAGGK
IQQETRRFDETTGQTILMRVKEGSDDYRYFPEPDIPAVDIDDDWIASVKKTIPEMPGSRRERYINEFGLTAYDAGVLTQT
KEMSDFFDATVAEGADPKLASNYLQGDVNAYLNEHKVDLLATDLTPTNLAGMIKMISDETISSKIAKKVFKAIMAGSEPV
QWVNDKGLVQLSDPAKLQPIVDDVLDNNQQSIDDFKNGKDRAVGFLVGQIMKKTRGQANPKVVNQLLMTSLKAR

Sequences:

>Translated_474_residues
MNFVTTIGLEVHVELKTNSKIFSPSPVQFGSEPNANTNVIDWGYPGVLPTPNKGVVEAGIKAATALHAEIEHHTYFDRKN
YFYPDNPKAYQITQHEKPIAHDGWIEIEVDGKKKKIGIEEMHIEEDAGKNTHENDYSYVDLNRQGTPLIEIVSKPDIASP
EEAYAYCEALRQRIQFTGVSDVKMEEGSMRVDVNISIRPAGSDKYGVKTEMKNLNSFNYVRKSLEYEEQRQQQVLMAGGK
IQQETRRFDETTGQTILMRVKEGSDDYRYFPEPDIPAVDIDDDWIASVKKTIPEMPGSRRERYINEFGLTAYDAGVLTQT
KEMSDFFDATVAEGADPKLASNYLQGDVNAYLNEHKVDLLATDLTPTNLAGMIKMISDETISSKIAKKVFKAIMAGSEPV
QWVNDKGLVQLSDPAKLQPIVDDVLDNNQQSIDDFKNGKDRAVGFLVGQIMKKTRGQANPKVVNQLLMTSLKAR
>Mature_474_residues
MNFVTTIGLEVHVELKTNSKIFSPSPVQFGSEPNANTNVIDWGYPGVLPTPNKGVVEAGIKAATALHAEIEHHTYFDRKN
YFYPDNPKAYQITQHEKPIAHDGWIEIEVDGKKKKIGIEEMHIEEDAGKNTHENDYSYVDLNRQGTPLIEIVSKPDIASP
EEAYAYCEALRQRIQFTGVSDVKMEEGSMRVDVNISIRPAGSDKYGVKTEMKNLNSFNYVRKSLEYEEQRQQQVLMAGGK
IQQETRRFDETTGQTILMRVKEGSDDYRYFPEPDIPAVDIDDDWIASVKKTIPEMPGSRRERYINEFGLTAYDAGVLTQT
KEMSDFFDATVAEGADPKLASNYLQGDVNAYLNEHKVDLLATDLTPTNLAGMIKMISDETISSKIAKKVFKAIMAGSEPV
QWVNDKGLVQLSDPAKLQPIVDDVLDNNQQSIDDFKNGKDRAVGFLVGQIMKKTRGQANPKVVNQLLMTSLKAR

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=490, Percent_Identity=34.6938775510204, Blast_Score=301, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI115534094, Length=342, Percent_Identity=35.3801169590643, Blast_Score=209, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6319391, Length=506, Percent_Identity=28.8537549407115, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI45550808, Length=490, Percent_Identity=32.4489795918367, Blast_Score=266, Evalue=3e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_LACPL (Q88XP6)

Other databases:

- EMBL:   AL935255
- RefSeq:   NP_784819.1
- ProteinModelPortal:   Q88XP6
- SMR:   Q88XP6
- GeneID:   1063228
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1149
- NMPDR:   fig|220668.1.peg.953
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   LPLA220668:LP_1149-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53194; Mature: 53194

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVTTIGLEVHVELKTNSKIFSPSPVQFGSEPNANTNVIDWGYPGVLPTPNKGVVEAGI
CCCEEEEEEEEEEEEECCCEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
KAATALHAEIEHHTYFDRKNYFYPDNPKAYQITQHEKPIAHDGWIEIEVDGKKKKIGIEE
HHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCHHH
MHIEEDAGKNTHENDYSYVDLNRQGTPLIEIVSKPDIASPEEAYAYCEALRQRIQFTGVS
EEECCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
DVKMEEGSMRVDVNISIRPAGSDKYGVKTEMKNLNSFNYVRKSLEYEEQRQQQVLMAGGK
CEEECCCCEEEEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
IQQETRRFDETTGQTILMRVKEGSDDYRYFPEPDIPAVDIDDDWIASVKKTIPEMPGSRR
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCHHH
ERYINEFGLTAYDAGVLTQTKEMSDFFDATVAEGADPKLASNYLQGDVNAYLNEHKVDLL
HHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHCCCCEEEE
ATDLTPTNLAGMIKMISDETISSKIAKKVFKAIMAGSEPVQWVNDKGLVQLSDPAKLQPI
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCHHH
VDDVLDNNQQSIDDFKNGKDRAVGFLVGQIMKKTRGQANPKVVNQLLMTSLKAR
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFVTTIGLEVHVELKTNSKIFSPSPVQFGSEPNANTNVIDWGYPGVLPTPNKGVVEAGI
CCCEEEEEEEEEEEEECCCEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHH
KAATALHAEIEHHTYFDRKNYFYPDNPKAYQITQHEKPIAHDGWIEIEVDGKKKKIGIEE
HHHHHHHHHHHHHEEECCCCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCCCCHHH
MHIEEDAGKNTHENDYSYVDLNRQGTPLIEIVSKPDIASPEEAYAYCEALRQRIQFTGVS
EEECCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCC
DVKMEEGSMRVDVNISIRPAGSDKYGVKTEMKNLNSFNYVRKSLEYEEQRQQQVLMAGGK
CEEECCCCEEEEEEEEEEECCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCC
IQQETRRFDETTGQTILMRVKEGSDDYRYFPEPDIPAVDIDDDWIASVKKTIPEMPGSRR
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCCHHH
ERYINEFGLTAYDAGVLTQTKEMSDFFDATVAEGADPKLASNYLQGDVNAYLNEHKVDLL
HHHHHHHCCCEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHCCCCEEEE
ATDLTPTNLAGMIKMISDETISSKIAKKVFKAIMAGSEPVQWVNDKGLVQLSDPAKLQPI
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCEEECCCCCCHHH
VDDVLDNNQQSIDDFKNGKDRAVGFLVGQIMKKTRGQANPKVVNQLLMTSLKAR
HHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566