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The map label for this gene is ligA

Identifier: 28377923

GI number: 28377923

Start: 1040841

End: 1042880

Strand: Direct

Name: ligA

Synonym: lp_1145

Alternate gene names: 28377923

Gene position: 1040841-1042880 (Clockwise)

Preceding gene: 28377922

Following gene: 28377924

Centisome position: 31.46

GC content: 49.07

Gene sequence:

>2040_bases
ATGGCGGACAAACCCAACACAATGACGGTTGAGCAGGCGGCTGATGAAGCGGCTGCTTTACGGCAAACGTTAAATGAATG
GCGTCGGCAGTACTATGACGAAGATGCGCCGAACGTTGAAGATAGTGTGTATGATCAAAAGTATGAACGGTTGGTCGAAC
TCGAACAAACTTTTCCACAGCTCGTTACACCGGATTCACCGACGCAATTAGTTGGTGGAACGGTTAAAGCAGGCTTTGAT
AAAGTGACCCATGAGATTCCGATGTTGTCATTGGGCGACGTCTTTTCACAAGCTGAGTTACAAGAGTTTGTTGATCGCTT
GGAACAAAACGTTGGTCATCCAGTCGATTATAATTGTGAGTTGAAAATTGATGGTTTAGCGCTGTCGTTACGTTACGAAG
ATGGGGTGCTCGTTCAAGGGTCCACGCGGGGTAACGGTACGGTTGGCGAAGATATTACGGCTAATATTAAGACAATCAAG
TCGATTCCACAACGGTTGACCCGACCACTGACGATCGATGTACGTGGGGAATGTTATATGCCTAAGGCAGCGTTTGCTGC
CTTGAATGAACGCCGAGAAGCAGCGGGAGAACCAGTCTTTGCGAATCCACGCAATGCTGCGGCCGGTACGTTACGGCAGC
TAGATTCACGAGTCGTGGCGGAACGACAGCTCAGCACGTTTATGTATAACATTGCCGACTATGAACCGTTAACCGCCCGT
ACGCAGAGTGACATGTTAACGGAGTTCGCAGATCTTGGTTTTGCCATCAATCCTGACTTTAAGGTGGCCCATTCGATGGC
CGATGTCTTTAGTTACATTGACCATTATCAAAATGAACGGCCAGAATTAGCATATGGCATTGATGGCATCGTGATCAAAG
CTAACCCGTTACCGCTGCAACGCTCACTAGGAGCGACAGTCAAGGTGCCACGATGGGCGATTGCCTTTAAGTTTCCACCT
GATGAGCAGCCGACGCTTTTAAAGGATGTTGAATGGACCGTTGGCCGTACAGGAGTCGTTACGCCAACTGCGGTTATGGA
ACCCGTTCAACTAGCTGGGACGACGGTTGCGCGGGCTTCGTTACACAATCCCGATTACGTGGCGGCCAAAGATGTCCGCA
TCGGTGATACGGTCTTGCTACACAAGGCTGGCGATATTATTCCGGAAATTTCATCGGTTGATTTAGCCAAACGACCCAAG
GATGCGCAACCATTAGTTATTCCAACCACGTGTCCGTCTTGTGGGGCGCCGCTGGTTCATTTGGAAGACGAAGTGGCTTT
ACGGTGTATTAACCCGAAATGTCCAGCACAAGTCCAAGAAGGGCTGGTTCATTTTGCCTCTCGCAATGCCATGAATATCG
ATGGCTTGGGACCACGAATCATTGCGCAACTCTATGCAAATCGGCTGGTTAGTGACGTTGCTGGTTTGTACCGATTAACT
AAGGCACAACTGTTAACATTAGATAAAATAAAAGATAAATCTGCTGAAAAGCTCTTGACAGCAATTGACCGTAGCCGTGA
TAATTCACTTGAACGGTTATTGTTCGGTCTTGGTATTCGGCACGTTGGTGCTAAAGTGGCGCGGTTAATCGCGCAGCATT
TTGGGACGATTGAGGCGTTGATGGCAGCTAGCCAAGAAGAGATTGCGGCGATCGATTCGATGGGCGATGTGATTGCGAAC
GCAGTGGTTCAATATTTTGAGAGCGACGAAGTTCATACACTCATCCGCGAATTGCAAGCAGTCAACGTGAATACCACTTA
TCAAGGCCCTAGTGCCACCGTGGCAGAAGATAGTAACAGTTGGTTTGCAGGCAAGCGGGTCGTGTTAACTGGTAAGCTGG
AAAGCTTTACAAGGCCAGATGCGACTCAATGGCTACAAGCTCACGGCGCCACGGTTATGGGAAGCGTCTCTAAAAAGACG
GATCTCGTGATTGCAGGTAGCGATGCCGGAAGCAAGTTGCAAAAAGCACAGCAGCTGGACATTACTGTATGGGATGAAGC
CCGGTTCAGCGAAACAATGAGGGAGGATGCACAAGCGTGA

Upstream 100 bases:

>100_bases
GCTGAACTAGACATTGCTTTCAAATCTGAAGGAATCAAGCGGTTATTAGCCGCATTTGCACCGATCCAAAAAGTACAGGA
CTAGTTAGGAGGACTGGCTG

Downstream 100 bases:

>100_bases
ATGTTAAACGCATACGCACGATTGGCTTAGTGGCAGTAGCTGCCATTGTTCTAGCCGCCTGCGGTAATTTAAAGAATTCG
TCCTTTGGGTCGACTAGTAG

Product: DNA ligase (NAD+)

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 679; Mature: 678

Protein sequence:

>679_residues
MADKPNTMTVEQAADEAAALRQTLNEWRRQYYDEDAPNVEDSVYDQKYERLVELEQTFPQLVTPDSPTQLVGGTVKAGFD
KVTHEIPMLSLGDVFSQAELQEFVDRLEQNVGHPVDYNCELKIDGLALSLRYEDGVLVQGSTRGNGTVGEDITANIKTIK
SIPQRLTRPLTIDVRGECYMPKAAFAALNERREAAGEPVFANPRNAAAGTLRQLDSRVVAERQLSTFMYNIADYEPLTAR
TQSDMLTEFADLGFAINPDFKVAHSMADVFSYIDHYQNERPELAYGIDGIVIKANPLPLQRSLGATVKVPRWAIAFKFPP
DEQPTLLKDVEWTVGRTGVVTPTAVMEPVQLAGTTVARASLHNPDYVAAKDVRIGDTVLLHKAGDIIPEISSVDLAKRPK
DAQPLVIPTTCPSCGAPLVHLEDEVALRCINPKCPAQVQEGLVHFASRNAMNIDGLGPRIIAQLYANRLVSDVAGLYRLT
KAQLLTLDKIKDKSAEKLLTAIDRSRDNSLERLLFGLGIRHVGAKVARLIAQHFGTIEALMAASQEEIAAIDSMGDVIAN
AVVQYFESDEVHTLIRELQAVNVNTTYQGPSATVAEDSNSWFAGKRVVLTGKLESFTRPDATQWLQAHGATVMGSVSKKT
DLVIAGSDAGSKLQKAQQLDITVWDEARFSETMREDAQA

Sequences:

>Translated_679_residues
MADKPNTMTVEQAADEAAALRQTLNEWRRQYYDEDAPNVEDSVYDQKYERLVELEQTFPQLVTPDSPTQLVGGTVKAGFD
KVTHEIPMLSLGDVFSQAELQEFVDRLEQNVGHPVDYNCELKIDGLALSLRYEDGVLVQGSTRGNGTVGEDITANIKTIK
SIPQRLTRPLTIDVRGECYMPKAAFAALNERREAAGEPVFANPRNAAAGTLRQLDSRVVAERQLSTFMYNIADYEPLTAR
TQSDMLTEFADLGFAINPDFKVAHSMADVFSYIDHYQNERPELAYGIDGIVIKANPLPLQRSLGATVKVPRWAIAFKFPP
DEQPTLLKDVEWTVGRTGVVTPTAVMEPVQLAGTTVARASLHNPDYVAAKDVRIGDTVLLHKAGDIIPEISSVDLAKRPK
DAQPLVIPTTCPSCGAPLVHLEDEVALRCINPKCPAQVQEGLVHFASRNAMNIDGLGPRIIAQLYANRLVSDVAGLYRLT
KAQLLTLDKIKDKSAEKLLTAIDRSRDNSLERLLFGLGIRHVGAKVARLIAQHFGTIEALMAASQEEIAAIDSMGDVIAN
AVVQYFESDEVHTLIRELQAVNVNTTYQGPSATVAEDSNSWFAGKRVVLTGKLESFTRPDATQWLQAHGATVMGSVSKKT
DLVIAGSDAGSKLQKAQQLDITVWDEARFSETMREDAQA
>Mature_678_residues
ADKPNTMTVEQAADEAAALRQTLNEWRRQYYDEDAPNVEDSVYDQKYERLVELEQTFPQLVTPDSPTQLVGGTVKAGFDK
VTHEIPMLSLGDVFSQAELQEFVDRLEQNVGHPVDYNCELKIDGLALSLRYEDGVLVQGSTRGNGTVGEDITANIKTIKS
IPQRLTRPLTIDVRGECYMPKAAFAALNERREAAGEPVFANPRNAAAGTLRQLDSRVVAERQLSTFMYNIADYEPLTART
QSDMLTEFADLGFAINPDFKVAHSMADVFSYIDHYQNERPELAYGIDGIVIKANPLPLQRSLGATVKVPRWAIAFKFPPD
EQPTLLKDVEWTVGRTGVVTPTAVMEPVQLAGTTVARASLHNPDYVAAKDVRIGDTVLLHKAGDIIPEISSVDLAKRPKD
AQPLVIPTTCPSCGAPLVHLEDEVALRCINPKCPAQVQEGLVHFASRNAMNIDGLGPRIIAQLYANRLVSDVAGLYRLTK
AQLLTLDKIKDKSAEKLLTAIDRSRDNSLERLLFGLGIRHVGAKVARLIAQHFGTIEALMAASQEEIAAIDSMGDVIANA
VVQYFESDEVHTLIRELQAVNVNTTYQGPSATVAEDSNSWFAGKRVVLTGKLESFTRPDATQWLQAHGATVMGSVSKKTD
LVIAGSDAGSKLQKAQQLDITVWDEARFSETMREDAQA

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=668, Percent_Identity=44.7604790419162, Blast_Score=554, Evalue=1e-159,
Organism=Escherichia coli, GI87082305, Length=583, Percent_Identity=24.1852487135506, Blast_Score=135, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_LACPL (Q88XQ0)

Other databases:

- EMBL:   AL935255
- RefSeq:   NP_784815.1
- HSSP:   O87703
- ProteinModelPortal:   Q88XQ0
- GeneID:   1063237
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1145
- NMPDR:   fig|220668.1.peg.949
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK2520148
- BioCyc:   LPLA220668:LP_1145-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74522; Mature: 74391

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 122-122 BINDING 120-120 BINDING 143-143 BINDING 177-177 BINDING 293-293 BINDING 317-317

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADKPNTMTVEQAADEAAALRQTLNEWRRQYYDEDAPNVEDSVYDQKYERLVELEQTFPQ
CCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LVTPDSPTQLVGGTVKAGFDKVTHEIPMLSLGDVFSQAELQEFVDRLEQNVGHPVDYNCE
HCCCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LKIDGLALSLRYEDGVLVQGSTRGNGTVGEDITANIKTIKSIPQRLTRPLTIDVRGECYM
EEECCEEEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEC
PKAAFAALNERREAAGEPVFANPRNAAAGTLRQLDSRVVAERQLSTFMYNIADYEPLTAR
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TQSDMLTEFADLGFAINPDFKVAHSMADVFSYIDHYQNERPELAYGIDGIVIKANPLPLQ
HHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCHH
RSLGATVKVPRWAIAFKFPPDEQPTLLKDVEWTVGRTGVVTPTAVMEPVQLAGTTVARAS
HHCCCEEECCCEEEEEECCCCCCCCHHHCCCHHCCCCCCCCHHHHHCCHHHHCCHHHHHH
LHNPDYVAAKDVRIGDTVLLHKAGDIIPEISSVDLAKRPKDAQPLVIPTTCPSCGAPLVH
CCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCHHCCCCCCCCEEEECCCCCCCCCEEE
LEDEVALRCINPKCPAQVQEGLVHFASRNAMNIDGLGPRIIAQLYANRLVSDVAGLYRLT
ECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KAQLLTLDKIKDKSAEKLLTAIDRSRDNSLERLLFGLGIRHVGAKVARLIAQHFGTIEAL
HHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAASQEEIAAIDSMGDVIANAVVQYFESDEVHTLIRELQAVNVNTTYQGPSATVAEDSNS
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCC
WFAGKRVVLTGKLESFTRPDATQWLQAHGATVMGSVSKKTDLVIAGSDAGSKLQKAQQLD
EECCCEEEEEECHHHCCCCCHHHHHHHCCCEEEECCCCCCEEEEECCCCCHHHHHHHHCC
ITVWDEARFSETMREDAQA
EEEECHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADKPNTMTVEQAADEAAALRQTLNEWRRQYYDEDAPNVEDSVYDQKYERLVELEQTFPQ
CCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LVTPDSPTQLVGGTVKAGFDKVTHEIPMLSLGDVFSQAELQEFVDRLEQNVGHPVDYNCE
HCCCCCCHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEE
LKIDGLALSLRYEDGVLVQGSTRGNGTVGEDITANIKTIKSIPQRLTRPLTIDVRGECYM
EEECCEEEEEEECCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCEEC
PKAAFAALNERREAAGEPVFANPRNAAAGTLRQLDSRVVAERQLSTFMYNIADYEPLTAR
CHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
TQSDMLTEFADLGFAINPDFKVAHSMADVFSYIDHYQNERPELAYGIDGIVIKANPLPLQ
HHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCHH
RSLGATVKVPRWAIAFKFPPDEQPTLLKDVEWTVGRTGVVTPTAVMEPVQLAGTTVARAS
HHCCCEEECCCEEEEEECCCCCCCCHHHCCCHHCCCCCCCCHHHHHCCHHHHCCHHHHHH
LHNPDYVAAKDVRIGDTVLLHKAGDIIPEISSVDLAKRPKDAQPLVIPTTCPSCGAPLVH
CCCCCCEEECCCCCCCEEEEECCCCCCCCCCCCCHHCCCCCCCCEEEECCCCCCCCCEEE
LEDEVALRCINPKCPAQVQEGLVHFASRNAMNIDGLGPRIIAQLYANRLVSDVAGLYRLT
ECCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KAQLLTLDKIKDKSAEKLLTAIDRSRDNSLERLLFGLGIRHVGAKVARLIAQHFGTIEAL
HHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAASQEEIAAIDSMGDVIANAVVQYFESDEVHTLIRELQAVNVNTTYQGPSATVAEDSNS
HHCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCEECCCCC
WFAGKRVVLTGKLESFTRPDATQWLQAHGATVMGSVSKKTDLVIAGSDAGSKLQKAQQLD
EECCCEEEEEECHHHCCCCCHHHHHHHCCCEEEECCCCCCEEEEECCCCCHHHHHHHHCC
ITVWDEARFSETMREDAQA
EEEECHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566