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The map label for this gene is pcrA [H]

Identifier: 28377922

GI number: 28377922

Start: 1038557

End: 1040824

Strand: Direct

Name: pcrA [H]

Synonym: lp_1144

Alternate gene names: 28377922

Gene position: 1038557-1040824 (Clockwise)

Preceding gene: 28377921

Following gene: 28377923

Centisome position: 31.39

GC content: 45.9

Gene sequence:

>2268_bases
GTGTCGAAAGAAAGCCTATTGGCAGGAATGAATGACAAGCAGCAAGAAGCCGTTTTAGACACAGAAGGACCGCTACTAAT
TATGGCCGGTGCGGGCTCGGGGAAGACCCGTGTATTGACGCACCGGGTAGCTTATTTAATTGAAGAGCAAGGGGTCAACC
CCTGGAATGTTTTAGCGATTACGTTTACGAACAAGGCCGCACGCGAAATGCGTGAACGGGTTGGTAAGCTGTTAGGTGAA
TCGGCGCAGGAGGTCTGGGTCTCAACGTTCCATGCCCTGTGTGTCCGAATTTTACGACGTGACATTGAACAAATCGGGTA
TAACCGAGCGTTCACAATCGCCGGCACTAGCGAGCAACGGACCTTAGTCAAACGAATCTTGACGGAACAAAATATCGATT
CGAAAAAATTTGATCCGCGGTCAATCTTATCCGCAATTTCAAATGCGAAAAATGACTTACAAACGCCGGAGATGTATAAA
GAATCGGCTGCCAGTCCCTTTGAGAGTGTCGTGGCAGACGTTTATTTGCAATACCAACGTGAATTAGCGGCTGATCAAGC
CGTTGACTTTGATGATCTGATTATGCTGACGATTCAATTATTCAAGCAGAATCCTGAAACGCTGGCGTGGTACCAAAACA
AATTCCATTATATTCACGTGGATGAATATCAAGATACTAATAATGCACAGTATACGTTAGTCAACTTGTTAGCTGACAAA
TATAAAAATTTATGTGTAGTTGGGGATGCGGATCAATCTATCTATGGTTGGCGGGGGGCCAACATGGAAAATATTCTCGA
TTTCGAAAAGGATTATCCGCAAGCTGACACGGTGATGTTGGAGCAAAACTATCGTTCGACCAAGACAATTTTGTCGGCGG
CTAATGCAGTTATTAACAATAATTCAACGCGGCGGCCGAAGAAATTATGGACTGAGAATGAACCGGGTGACAAGATTTCT
TATTATCGTGGTCAGAGTGAAAGTGATGAAACTCACTTTGTCATTGCCAAGATTCAAGAAAGCATGCAGCAGGACAAAAT
GGACTACGGCGATTTTGCGATCCTTTATCGGACGAACGCACAATCCCGGGTAATGGAAGAGTCACTCGTTAAGGCGAACA
TCCCTTATACAATGGTCGGTGGTAATAAGTTCTATGACCGTAAAGAAATTCGGGACGTCTTATCCTACTTGACTTTGGCA
GTGAACGATCAAGATTCAATGAACTTTGAACGGGTCGTTAATGAACCTAAGCGCGGCATTGGTCAAACGAGTGTTGAAAA
ATTGCGGTTGTTCGCGGATGAAGCGGGTTATTCCATGTTGGAAGCAGCTAGCAATGTTGACATGGCTAATGGCATTTCAG
CACGAGCGAAGGGTGCTTTAGCGAAGTTTGCAGCGACGATGAATGAATTGCGCGCTATGCGGGAATACTTATCCGTGACG
GATTTAACACAAGAGATTCTTGACCGAACGGGTTACATGAAGGCGTTGAAAGATGCTAAGGCAACGAAATCGTTAGAGGC
ACAAGCCCGTATCGAAAACATTGAAGAATTCTTGTCTGTCACCAAACAATTCGATGATAGTTATCAACCTGAAGATGAAG
ATAGTGACAAATTCGTGGACTTCTTAGCGGATTTAGCACTGGTTTCGGATGTGGATAACGTTGAGGAAGAACCCCAAGCC
GTGACGCTGATGACGTTACATGCAGCGAAGGGGCTAGAATTTCCGGTTGTTTTCTTGATTGGGATGGAAGAAGGTATCTT
CCCACTGTCCCGGGCAATGATGGATGAAGACGAGCTGGAAGAAGAACGGCGGTTAGCATACGTTGGGATTACGCGGGCTG
AGAAGAAGCTGTACTTGACCAATGCATACTCACGGATGCTTTACGGTCGGCGGCAGAATAATCCGGCTTCCCGTTTCGTT
GAAGAAATCGAACCAGAATTACTCAAAAATGAAAATACGTTGGATGGTGCAAGTAGCACTGGTCCTAAAGTCCCATTTGG
TGCGGATCGTGAACGGCGTGCTTTTACACCTTCATATAAAAAGAATCCAGTGGGGACGCGGGCCACGGTCAAGAAGGCTA
GTGGCACGGGTGCTGACAAGAATAGTTGGTCGATCGGTGATAAAGTCAAGCATCGTAAGTGGGGAACCGGAACGGTCGTG
AAGGTCTCCGGTGCTGGTGAAGATGCTGAACTAGACATTGCTTTCAAATCTGAAGGAATCAAGCGGTTATTAGCCGCATT
TGCACCGATCCAAAAAGTACAGGACTAG

Upstream 100 bases:

>100_bases
CGCGATTTTTGTATGAGATGCTGACCTTAAAGTACAGCAATCACGGCCAAAATTTGCTATAATGTTTGGTGACTGATTTT
TAGAAAGGGTGGCGAACAGC

Downstream 100 bases:

>100_bases
TTAGGAGGACTGGCTGATGGCGGACAAACCCAACACAATGACGGTTGAGCAGGCGGCTGATGAAGCGGCTGCTTTACGGC
AAACGTTAAATGAATGGCGT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 755; Mature: 754

Protein sequence:

>755_residues
MSKESLLAGMNDKQQEAVLDTEGPLLIMAGAGSGKTRVLTHRVAYLIEEQGVNPWNVLAITFTNKAAREMRERVGKLLGE
SAQEVWVSTFHALCVRILRRDIEQIGYNRAFTIAGTSEQRTLVKRILTEQNIDSKKFDPRSILSAISNAKNDLQTPEMYK
ESAASPFESVVADVYLQYQRELAADQAVDFDDLIMLTIQLFKQNPETLAWYQNKFHYIHVDEYQDTNNAQYTLVNLLADK
YKNLCVVGDADQSIYGWRGANMENILDFEKDYPQADTVMLEQNYRSTKTILSAANAVINNNSTRRPKKLWTENEPGDKIS
YYRGQSESDETHFVIAKIQESMQQDKMDYGDFAILYRTNAQSRVMEESLVKANIPYTMVGGNKFYDRKEIRDVLSYLTLA
VNDQDSMNFERVVNEPKRGIGQTSVEKLRLFADEAGYSMLEAASNVDMANGISARAKGALAKFAATMNELRAMREYLSVT
DLTQEILDRTGYMKALKDAKATKSLEAQARIENIEEFLSVTKQFDDSYQPEDEDSDKFVDFLADLALVSDVDNVEEEPQA
VTLMTLHAAKGLEFPVVFLIGMEEGIFPLSRAMMDEDELEEERRLAYVGITRAEKKLYLTNAYSRMLYGRRQNNPASRFV
EEIEPELLKNENTLDGASSTGPKVPFGADRERRAFTPSYKKNPVGTRATVKKASGTGADKNSWSIGDKVKHRKWGTGTVV
KVSGAGEDAELDIAFKSEGIKRLLAAFAPIQKVQD

Sequences:

>Translated_755_residues
MSKESLLAGMNDKQQEAVLDTEGPLLIMAGAGSGKTRVLTHRVAYLIEEQGVNPWNVLAITFTNKAAREMRERVGKLLGE
SAQEVWVSTFHALCVRILRRDIEQIGYNRAFTIAGTSEQRTLVKRILTEQNIDSKKFDPRSILSAISNAKNDLQTPEMYK
ESAASPFESVVADVYLQYQRELAADQAVDFDDLIMLTIQLFKQNPETLAWYQNKFHYIHVDEYQDTNNAQYTLVNLLADK
YKNLCVVGDADQSIYGWRGANMENILDFEKDYPQADTVMLEQNYRSTKTILSAANAVINNNSTRRPKKLWTENEPGDKIS
YYRGQSESDETHFVIAKIQESMQQDKMDYGDFAILYRTNAQSRVMEESLVKANIPYTMVGGNKFYDRKEIRDVLSYLTLA
VNDQDSMNFERVVNEPKRGIGQTSVEKLRLFADEAGYSMLEAASNVDMANGISARAKGALAKFAATMNELRAMREYLSVT
DLTQEILDRTGYMKALKDAKATKSLEAQARIENIEEFLSVTKQFDDSYQPEDEDSDKFVDFLADLALVSDVDNVEEEPQA
VTLMTLHAAKGLEFPVVFLIGMEEGIFPLSRAMMDEDELEEERRLAYVGITRAEKKLYLTNAYSRMLYGRRQNNPASRFV
EEIEPELLKNENTLDGASSTGPKVPFGADRERRAFTPSYKKNPVGTRATVKKASGTGADKNSWSIGDKVKHRKWGTGTVV
KVSGAGEDAELDIAFKSEGIKRLLAAFAPIQKVQD
>Mature_754_residues
SKESLLAGMNDKQQEAVLDTEGPLLIMAGAGSGKTRVLTHRVAYLIEEQGVNPWNVLAITFTNKAAREMRERVGKLLGES
AQEVWVSTFHALCVRILRRDIEQIGYNRAFTIAGTSEQRTLVKRILTEQNIDSKKFDPRSILSAISNAKNDLQTPEMYKE
SAASPFESVVADVYLQYQRELAADQAVDFDDLIMLTIQLFKQNPETLAWYQNKFHYIHVDEYQDTNNAQYTLVNLLADKY
KNLCVVGDADQSIYGWRGANMENILDFEKDYPQADTVMLEQNYRSTKTILSAANAVINNNSTRRPKKLWTENEPGDKISY
YRGQSESDETHFVIAKIQESMQQDKMDYGDFAILYRTNAQSRVMEESLVKANIPYTMVGGNKFYDRKEIRDVLSYLTLAV
NDQDSMNFERVVNEPKRGIGQTSVEKLRLFADEAGYSMLEAASNVDMANGISARAKGALAKFAATMNELRAMREYLSVTD
LTQEILDRTGYMKALKDAKATKSLEAQARIENIEEFLSVTKQFDDSYQPEDEDSDKFVDFLADLALVSDVDNVEEEPQAV
TLMTLHAAKGLEFPVVFLIGMEEGIFPLSRAMMDEDELEEERRLAYVGITRAEKKLYLTNAYSRMLYGRRQNNPASRFVE
EIEPELLKNENTLDGASSTGPKVPFGADRERRAFTPSYKKNPVGTRATVKKASGTGADKNSWSIGDKVKHRKWGTGTVVK
VSGAGEDAELDIAFKSEGIKRLLAAFAPIQKVQD

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=755, Percent_Identity=41.5894039735099, Blast_Score=550, Evalue=1e-158,
Organism=Escherichia coli, GI48994965, Length=636, Percent_Identity=39.7798742138365, Blast_Score=420, Evalue=1e-118,
Organism=Escherichia coli, GI1787196, Length=339, Percent_Identity=26.5486725663717, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=737, Percent_Identity=27.8154681139756, Blast_Score=239, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6324477, Length=667, Percent_Identity=25.0374812593703, Blast_Score=138, Evalue=4e-33,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 85092; Mature: 84961

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKESLLAGMNDKQQEAVLDTEGPLLIMAGAGSGKTRVLTHRVAYLIEEQGVNPWNVLAI
CCCHHHHCCCCCCHHHHEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAREMRERVGKLLGESAQEVWVSTFHALCVRILRRDIEQIGYNRAFTIAGTSEQR
EECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
TLVKRILTEQNIDSKKFDPRSILSAISNAKNDLQTPEMYKESAASPFESVVADVYLQYQR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
ELAADQAVDFDDLIMLTIQLFKQNPETLAWYQNKFHYIHVDEYQDTNNAQYTLVNLLADK
HHHHHCCCCHHHHHHHHHHHHHCCCCCEEHCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
YKNLCVVGDADQSIYGWRGANMENILDFEKDYPQADTVMLEQNYRSTKTILSAANAVINN
HCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCC
NSTRRPKKLWTENEPGDKISYYRGQSESDETHFVIAKIQESMQQDKMDYGDFAILYRTNA
CCCCCCHHHCCCCCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCC
QSRVMEESLVKANIPYTMVGGNKFYDRKEIRDVLSYLTLAVNDQDSMNFERVVNEPKRGI
HHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCHHHHHCCHHHCC
GQTSVEKLRLFADEAGYSMLEAASNVDMANGISARAKGALAKFAATMNELRAMREYLSVT
CHHHHHHHHHHHHHCCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLTQEILDRTGYMKALKDAKATKSLEAQARIENIEEFLSVTKQFDDSYQPEDEDSDKFVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
FLADLALVSDVDNVEEEPQAVTLMTLHAAKGLEFPVVFLIGMEEGIFPLSRAMMDEDELE
HHHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCCCEEEEEECCCCCHHHHHHHCCHHHHH
EERRLAYVGITRAEKKLYLTNAYSRMLYGRRQNNPASRFVEEIEPELLKNENTLDGASST
HHHCEEEEEEECCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCC
GPKVPFGADRERRAFTPSYKKNPVGTRATVKKASGTGADKNSWSIGDKVKHRKWGTGTVV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCCCCCEE
KVSGAGEDAELDIAFKSEGIKRLLAAFAPIQKVQD
EEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKESLLAGMNDKQQEAVLDTEGPLLIMAGAGSGKTRVLTHRVAYLIEEQGVNPWNVLAI
CCHHHHCCCCCCHHHHEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAREMRERVGKLLGESAQEVWVSTFHALCVRILRRDIEQIGYNRAFTIAGTSEQR
EECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHH
TLVKRILTEQNIDSKKFDPRSILSAISNAKNDLQTPEMYKESAASPFESVVADVYLQYQR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHH
ELAADQAVDFDDLIMLTIQLFKQNPETLAWYQNKFHYIHVDEYQDTNNAQYTLVNLLADK
HHHHHCCCCHHHHHHHHHHHHHCCCCCEEHCCCCEEEEEEECCCCCCCCHHHHHHHHHHH
YKNLCVVGDADQSIYGWRGANMENILDFEKDYPQADTVMLEQNYRSTKTILSAANAVINN
HCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHCC
NSTRRPKKLWTENEPGDKISYYRGQSESDETHFVIAKIQESMQQDKMDYGDFAILYRTNA
CCCCCCHHHCCCCCCCCCEEEECCCCCCCCCCEEHHHHHHHHHHCCCCCCCEEEEEECCC
QSRVMEESLVKANIPYTMVGGNKFYDRKEIRDVLSYLTLAVNDQDSMNFERVVNEPKRGI
HHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHEEECCCCCCCHHHHHCCHHHCC
GQTSVEKLRLFADEAGYSMLEAASNVDMANGISARAKGALAKFAATMNELRAMREYLSVT
CHHHHHHHHHHHHHCCHHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
DLTQEILDRTGYMKALKDAKATKSLEAQARIENIEEFLSVTKQFDDSYQPEDEDSDKFVD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
FLADLALVSDVDNVEEEPQAVTLMTLHAAKGLEFPVVFLIGMEEGIFPLSRAMMDEDELE
HHHHHHHHHHHHHHCCCCCEEEEEEEEHHCCCCCCEEEEEECCCCCHHHHHHHCCHHHHH
EERRLAYVGITRAEKKLYLTNAYSRMLYGRRQNNPASRFVEEIEPELLKNENTLDGASST
HHHCEEEEEEECCCCEEEEHHHHHHHHHCCCCCCHHHHHHHHHCHHHHCCCCCCCCCCCC
GPKVPFGADRERRAFTPSYKKNPVGTRATVKKASGTGADKNSWSIGDKVKHRKWGTGTVV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHHCCCCCCCEE
KVSGAGEDAELDIAFKSEGIKRLLAAFAPIQKVQD
EEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA