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The map label for this gene is purK2 [H]

Identifier: 28377921

GI number: 28377921

Start: 1036947

End: 1038116

Strand: Direct

Name: purK2 [H]

Synonym: lp_1141

Alternate gene names: 28377921

Gene position: 1036947-1038116 (Clockwise)

Preceding gene: 28377920

Following gene: 28377922

Centisome position: 31.34

GC content: 47.18

Gene sequence:

>1170_bases
GTGGACAACTCGATTTTAAACCCAGGTGGTACGCTTGGCATTATTGGAAGTAGTACTAATGGTGTTGGATTAGTAATTGC
CGCGCGTAATGCTGGCATCAATGTCGGGGTATACGGCGATAACGAAAATACTGAGACCATGGAACTGGCAGATTTTCGCG
TTGTCGGGGCCCTCAATGATCAACAACAGTTACAGAACTTCGCAGAACGTTGTGATATGGTGACTTACGAGTCGGAAACC
ATTGATGCGTCGGTCATTAATTTTTTGGCGGCCCATACCCGGGTTCCACAAGGCGCCGATGCGCTTGAGATTACACAGGA
CCGCTCTTTGGAACGGGCGTTTTTCAGCCAACTTAACTTGAATGTAGCACCGTCAGCGACAATTGTCAGTTTGGATGATG
TCTATCAATCGATCGGGTCAATTGGATATCCTAGTATTTTGAAGCCGATTCAAAAGGGCTTGGGTCAAAATCGGCAACTG
GTCATTAAGACGCAGACGGATATTGTCAAGGCGGCTGATCTGTTGGATTGGGGCACATACTTGTTAGAGTCATTGATTCC
ATATGATAAGGAACTCTCCGTTTTTGGCGCTAAGAGTGGTGATACGACAGCTTTCTTCCCAACCGTTGAAAACCGGTATC
GTAATCATGAGCTAGTTGCCTCGATTGTGCCAGCTCAAATTGATTCAGCTGTGAATGACGAAATGCTGCGGATCACAAAG
GAAATTAGTGATAACTTGAGTTATACGGGTGTCTTTGAAGTTGCCTTCTTCTTAACGAAGAGTGGTAATTTATACGTGAA
GCGGATTGTCCCTGCGATGCATCAGGCGGGTTATGTCTTCGAACGTGCTACGAATATTAGTATGGCAGAACAACACTTGC
GAGCAATTGCGGGACTGCCGTTAATGCCAGTCAAGCAGTTGATGCCCGTGGTTGCTGCTTACTTTACACCGGCTCAGGAG
AGTGGCATTCGCACGCAGTGGCAGATTAAGACGAACTGGTTCTTTAATTTCTATCACCGAACGTTGTTACAAGCGCACCG
GGCACCAGTCGCTGGCCACGTGCTAGTCGAAGCACCAAGTGTTAAAGAAGCACTCGAACAGATCGAAGACACAAGTATTT
GGCAGTTTGGGCAAACGACGCCGGCTGATAATGAACCTGAAGCTGAGTAG

Upstream 100 bases:

>100_bases
TGTGCGTGTCAGCAAGTATGATTGTTACAGGATAATGAGCGTTAAAATAATTCAATAATCAGATTATAATTATTTAAAGC
ATAGGAAAGTAGGGACTGAA

Downstream 100 bases:

>100_bases
TGTATGATCAGAACCTAGTTTTACGGATTATTACGGTTAAACTAATCGTACTGTTCGCACCGGGTGACCAGCTATTATGG
GTAACCGTCGTGAATAAATA

Product: phosphoribosylaminoimidazole carboxylase, ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MDNSILNPGGTLGIIGSSTNGVGLVIAARNAGINVGVYGDNENTETMELADFRVVGALNDQQQLQNFAERCDMVTYESET
IDASVINFLAAHTRVPQGADALEITQDRSLERAFFSQLNLNVAPSATIVSLDDVYQSIGSIGYPSILKPIQKGLGQNRQL
VIKTQTDIVKAADLLDWGTYLLESLIPYDKELSVFGAKSGDTTAFFPTVENRYRNHELVASIVPAQIDSAVNDEMLRITK
EISDNLSYTGVFEVAFFLTKSGNLYVKRIVPAMHQAGYVFERATNISMAEQHLRAIAGLPLMPVKQLMPVVAAYFTPAQE
SGIRTQWQIKTNWFFNFYHRTLLQAHRAPVAGHVLVEAPSVKEALEQIEDTSIWQFGQTTPADNEPEAE

Sequences:

>Translated_389_residues
MDNSILNPGGTLGIIGSSTNGVGLVIAARNAGINVGVYGDNENTETMELADFRVVGALNDQQQLQNFAERCDMVTYESET
IDASVINFLAAHTRVPQGADALEITQDRSLERAFFSQLNLNVAPSATIVSLDDVYQSIGSIGYPSILKPIQKGLGQNRQL
VIKTQTDIVKAADLLDWGTYLLESLIPYDKELSVFGAKSGDTTAFFPTVENRYRNHELVASIVPAQIDSAVNDEMLRITK
EISDNLSYTGVFEVAFFLTKSGNLYVKRIVPAMHQAGYVFERATNISMAEQHLRAIAGLPLMPVKQLMPVVAAYFTPAQE
SGIRTQWQIKTNWFFNFYHRTLLQAHRAPVAGHVLVEAPSVKEALEQIEDTSIWQFGQTTPADNEPEAE
>Mature_389_residues
MDNSILNPGGTLGIIGSSTNGVGLVIAARNAGINVGVYGDNENTETMELADFRVVGALNDQQQLQNFAERCDMVTYESET
IDASVINFLAAHTRVPQGADALEITQDRSLERAFFSQLNLNVAPSATIVSLDDVYQSIGSIGYPSILKPIQKGLGQNRQL
VIKTQTDIVKAADLLDWGTYLLESLIPYDKELSVFGAKSGDTTAFFPTVENRYRNHELVASIVPAQIDSAVNDEMLRITK
EISDNLSYTGVFEVAFFLTKSGNLYVKRIVPAMHQAGYVFERATNISMAEQHLRAIAGLPLMPVKQLMPVVAAYFTPAQE
SGIRTQWQIKTNWFFNFYHRTLLQAHRAPVAGHVLVEAPSVKEALEQIEDTSIWQFGQTTPADNEPEAE

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=204, Percent_Identity=26.4705882352941, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788155, Length=329, Percent_Identity=22.1884498480243, Blast_Score=62, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324702, Length=302, Percent_Identity=24.8344370860927, Blast_Score=88, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006115
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp; PF03446 NAD_binding_2 [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 42833; Mature: 42833

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNSILNPGGTLGIIGSSTNGVGLVIAARNAGINVGVYGDNENTETMELADFRVVGALND
CCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEEEEEEECCCC
QQQLQNFAERCDMVTYESETIDASVINFLAAHTRVPQGADALEITQDRSLERAFFSQLNL
HHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHCCC
NVAPSATIVSLDDVYQSIGSIGYPSILKPIQKGLGQNRQLVIKTQTDIVKAADLLDWGTY
CCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHH
LLESLIPYDKELSVFGAKSGDTTAFFPTVENRYRNHELVASIVPAQIDSAVNDEMLRITK
HHHHHCCCCCCEEEEECCCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EISDNLSYTGVFEVAFFLTKSGNLYVKRIVPAMHQAGYVFERATNISMAEQHLRAIAGLP
HHHCCCCCCHHHHEEEEEECCCCEEEEHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHCCC
LMPVKQLMPVVAAYFTPAQESGIRTQWQIKTNWFFNFYHRTLLQAHRAPVAGHVLVEAPS
CCCHHHHHHHHHHHCCCCHHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCC
VKEALEQIEDTSIWQFGQTTPADNEPEAE
HHHHHHHHCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MDNSILNPGGTLGIIGSSTNGVGLVIAARNAGINVGVYGDNENTETMELADFRVVGALND
CCCCCCCCCCCEEEEECCCCCEEEEEEECCCCEEEEEECCCCCCCEEEEEEEEEEECCCC
QQQLQNFAERCDMVTYESETIDASVINFLAAHTRVPQGADALEITQDRSLERAFFSQLNL
HHHHHHHHHHCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHCCC
NVAPSATIVSLDDVYQSIGSIGYPSILKPIQKGLGQNRQLVIKTQTDIVKAADLLDWGTY
CCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHH
LLESLIPYDKELSVFGAKSGDTTAFFPTVENRYRNHELVASIVPAQIDSAVNDEMLRITK
HHHHHCCCCCCEEEEECCCCCCEEEECCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
EISDNLSYTGVFEVAFFLTKSGNLYVKRIVPAMHQAGYVFERATNISMAEQHLRAIAGLP
HHHCCCCCCHHHHEEEEEECCCCEEEEHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHCCC
LMPVKQLMPVVAAYFTPAQESGIRTQWQIKTNWFFNFYHRTLLQAHRAPVAGHVLVEAPS
CCCHHHHHHHHHHHCCCCHHCCCCEEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEECCC
VKEALEQIEDTSIWQFGQTTPADNEPEAE
HHHHHHHHCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377 [H]