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The map label for this gene is rpoA

Identifier: 28377858

GI number: 28377858

Start: 965520

End: 966464

Strand: Direct

Name: rpoA

Synonym: lp_1062

Alternate gene names: 28377858

Gene position: 965520-966464 (Clockwise)

Preceding gene: 28377857

Following gene: 28377859

Centisome position: 29.19

GC content: 41.27

Gene sequence:

>945_bases
ATGATTGAATTTGAAAAACCTAACATTCATAAAATTGATGAAAACGACAACTATGGTAAGTTTGTTGTAGAACCGCTTGA
ACGCGGTTATGGTACAACTTTAGGGAATTCACTTCGTCGGATTCTTCTTTCTTCTTTACCTGGCGCTGCTGTTACTAGTA
TTCAAATTGATGGTGTTCTTCATGAATTTTCAACGATTGAGGGCGTAACGGAAGACGTTACGGCAATTATCTTGAATGTT
AAGAAGATTGCACTTAAGTTGGAATCAGACGAAACCAAGACGTTGGAAATCGACGTTAAGGGTCCTGCTAACGTTACTGC
CGGTGATATCATTGGCGATGCGGACGTAGAAGTCTTGAATCCAGACTTACCAATTTGTACCGTAGCAGACGGGGCACACT
TCCATATGCGTATGACCGCAAATACTGGTCGTGGTTATGTTTCCGCTGAGGATAACAAACATCGTGAAGATGACATGCCA
ATTGGCGTTTTAGCTGTTGATTCATTGTATTCTCCAATCGAACGTGTCAACTACCAAGTTGAAAACACGCGGGTTGGTCA
ACGTGATGATTTCGATAAGTTAACCTTAGACGTTTGGACAAATGGTTCAATCACTCCAAGTGAAGCCATTAGTCTATCAG
CCAAAATCCTGACTGATCACCTTTCAATCTTCGTAAATCTCACTGATGAAGCTAAAAACACTGATGTGATGGTCGAGAAG
GAAGAAACGCATAAGGAAAAGATGTTAGAAATGACGATTGAAGAGTTAGATCTCTCCGTCCGTTCATACAATTGCTTGAA
GCGTGCTGGCATCAACACGGTCCAAGAATTAACTAACAAAACTGAAGCTGATATGATGAAGGTTCGCAACCTTGGACGCA
AGTCACTCGAGGAAGTTAAGGCAAAATTAGCTGACCTTGGGTTATCATTACGCAAAGAAGACTAA

Upstream 100 bases:

>100_bases
GTCGTCGTGTTTAATCTGTGACGTTCATTTTGAGGGCAAACTTCATTTTGTACGCACAACTACTAAACACCACGCGTTTT
GAAAGGGGTAAAAGATAAGA

Downstream 100 bases:

>100_bases
TACAAGGGAGGATATCCGTTCATGAGTTACCGTAAATTACAACGTACAAGTTCACAACGTCGTGCCTTATTACGCGATTT
GACAACGTCATTGATCGTTA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MIEFEKPNIHKIDENDNYGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVTSIQIDGVLHEFSTIEGVTEDVTAIILNV
KKIALKLESDETKTLEIDVKGPANVTAGDIIGDADVEVLNPDLPICTVADGAHFHMRMTANTGRGYVSAEDNKHREDDMP
IGVLAVDSLYSPIERVNYQVENTRVGQRDDFDKLTLDVWTNGSITPSEAISLSAKILTDHLSIFVNLTDEAKNTDVMVEK
EETHKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELTNKTEADMMKVRNLGRKSLEEVKAKLADLGLSLRKED

Sequences:

>Translated_314_residues
MIEFEKPNIHKIDENDNYGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVTSIQIDGVLHEFSTIEGVTEDVTAIILNV
KKIALKLESDETKTLEIDVKGPANVTAGDIIGDADVEVLNPDLPICTVADGAHFHMRMTANTGRGYVSAEDNKHREDDMP
IGVLAVDSLYSPIERVNYQVENTRVGQRDDFDKLTLDVWTNGSITPSEAISLSAKILTDHLSIFVNLTDEAKNTDVMVEK
EETHKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELTNKTEADMMKVRNLGRKSLEEVKAKLADLGLSLRKED
>Mature_314_residues
MIEFEKPNIHKIDENDNYGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVTSIQIDGVLHEFSTIEGVTEDVTAIILNV
KKIALKLESDETKTLEIDVKGPANVTAGDIIGDADVEVLNPDLPICTVADGAHFHMRMTANTGRGYVSAEDNKHREDDMP
IGVLAVDSLYSPIERVNYQVENTRVGQRDDFDKLTLDVWTNGSITPSEAISLSAKILTDHLSIFVNLTDEAKNTDVMVEK
EETHKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELTNKTEADMMKVRNLGRKSLEEVKAKLADLGLSLRKED

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=311, Percent_Identity=47.2668810289389, Blast_Score=270, Evalue=6e-74,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_LACPL (Q88XW0)

Other databases:

- EMBL:   AL935254
- RefSeq:   NP_784750.1
- ProteinModelPortal:   Q88XW0
- GeneID:   1063463
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_1062
- NMPDR:   fig|220668.1.peg.884
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   LPLA220668:LP_1062-MONOMER
- BRENDA:   2.7.7.6
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 34843; Mature: 34843

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFEKPNIHKIDENDNYGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVTSIQIDGVL
CCCCCCCCCEECCCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHH
HEFSTIEGVTEDVTAIILNVKKIALKLESDETKTLEIDVKGPANVTAGDIIGDADVEVLN
HHHHHHCCCHHHHHHHHHEEEHEEEEEECCCCEEEEEEECCCCCCCCCCEECCCCCEEEC
PDLPICTVADGAHFHMRMTANTGRGYVSAEDNKHREDDMPIGVLAVDSLYSPIERVNYQV
CCCCEEEEECCCEEEEEEEECCCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHCCEEE
ENTRVGQRDDFDKLTLDVWTNGSITPSEAISLSAKILTDHLSIFVNLTDEAKNTDVMVEK
ECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEEEC
EETHKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELTNKTEADMMKVRNLGRKSLEEVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
AKLADLGLSLRKED
HHHHHCCCEEECCC
>Mature Secondary Structure
MIEFEKPNIHKIDENDNYGKFVVEPLERGYGTTLGNSLRRILLSSLPGAAVTSIQIDGVL
CCCCCCCCCEECCCCCCCCCEEEEHHHCCCCCHHHHHHHHHHHHHCCCCEEEEEEECHHH
HEFSTIEGVTEDVTAIILNVKKIALKLESDETKTLEIDVKGPANVTAGDIIGDADVEVLN
HHHHHHCCCHHHHHHHHHEEEHEEEEEECCCCEEEEEEECCCCCCCCCCEECCCCCEEEC
PDLPICTVADGAHFHMRMTANTGRGYVSAEDNKHREDDMPIGVLAVDSLYSPIERVNYQV
CCCCEEEEECCCEEEEEEEECCCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHCCEEE
ENTRVGQRDDFDKLTLDVWTNGSITPSEAISLSAKILTDHLSIFVNLTDEAKNTDVMVEK
ECCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHEEEEEEEEECCCCCCCCEEEEC
EETHKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELTNKTEADMMKVRNLGRKSLEEVK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHHCCHHHHHHHH
AKLADLGLSLRKED
HHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566