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The map label for this gene is murF [H]

Identifier: 28377406

GI number: 28377406

Start: 463650

End: 465038

Strand: Direct

Name: murF [H]

Synonym: lp_0518

Alternate gene names: 28377406

Gene position: 463650-465038 (Clockwise)

Preceding gene: 28377405

Following gene: 28377407

Centisome position: 14.01

GC content: 48.38

Gene sequence:

>1389_bases
ATGAAGATGCAAGTTGCCGAAATTGCTAAGGCCGTTCAAGCGGTCAATGCCGAACAGGCGTGGCCCGATGTTAATGTAAC
GGGTGTGGCGTTTGATAGCCGAAAGCTCCAAGCAGGGGATCTATTTATTCCGCTGATGGGCGCTAATGATGGTCATCAAT
ATATTCAAAGTGCAATCGAGCACGGGGCCGTTGCTACCTTATGGGCCAGCGACCATGCCGACGACGTTCCAGAAGACTTA
CCTGTCATTATGGTTGCTGATACATTGACGGCACTACAGCAGCTGGGGCAATACTATTTACAAAAAATTAATCCGAAAGT
CGTGGCTGTTACGGGTAGTAATGGTAAAACAACGACGAAGGATATGATTGCGAGTGTTTTGAGCACTCAGTTTAACGTGA
CAAAGACGCACGCCAACTTTAATAATCAGATTGGTGTGCCAGTGACGTTACTCAGTATGGAACCGAATACAGAAGTGGTC
GTCGTTGAAATGGGGATGGACCATTTTGGCGAGTTGGATTTCTTGAGCCGTCTCGTTCAACCAGACGTTGCGGTGATTAC
CATGATTGGGGAAGCCCACATTGAGTTCTTTGGTACACGGGATCGAATTGCTGATGCCAAAATGGAAATTGTCCACGGCT
TAAAAGAAGATGGCACGTTCATTTTTAATGGTGATGAACCACTGCTACAAGAACGTGCTAAGGAAGTCAGTCAGCATCAA
CGGACGTTTGGGTTGGATGATACGAACACCTTAGCAGGCAGTGATATCACGACTAGTCGGGCTCAAACGCAATTTAAGAC
GAGTCTGTGGCCGGACAACACGTACACAATTCCGATGATGGGTGCGTACAATGTCAATAATGCATTGGCGGCACTCTTGG
TAGCTGATACTTTTCACATTCGACCAGCCGCTGCGCAACAAGCAATCGCCAGTTTTGTGCCGACGGAGAACCGGACGGAA
TGGTTAACTGGTTACCGTGGAGAAGCCATCTTAAGTGATGTTTATAACTCTAATCCAACGGCAGCTCGACGGGTCCTAGC
CGCTTTTGCGGCGGTACCTACTAAGGGTCAGCGAATCGCTGTACTCGGTGATATGCTTGAGTTGGGTGACCAGTCGGATG
CACTACACATGAGTTTAGCCAGTGAATTGGATCCAGCCACGATTGCCAGCGTCTATTTGAATGGTGAACATATGCGAGCG
TTGGCAGAAGTGCTACAAGAAAAGTACCCAGCCGGTGCGGTCCACTATTATCAAACGGATCAACAACCCCAATTGATTGC
CGACTTAACGGCGACGGTTAGTGCGGATGATCAGGTCTTATTGAAGGGGAGTCACGGGATTCACCTTGAAAAGGTTCTGA
CGGCGCTGGAAGCTGCTCCTGAAAAATAA

Upstream 100 bases:

>100_bases
TGCGCGTAGCTTGAATTGGGCTACGATTCGAGAAAGTGGAACCTTAGTCAGAACTTGGCTGACCGACAAGTGGTCGCCAT
GAATGGAGAGTTTAATCGAT

Downstream 100 bases:

>100_bases
AATTTAAGTGATTCGATTAAGCAGGCCCCTCGCAATGACTGGGTAATGGTGACGCTAACATTAGACTGATAATGTTGTTT
CCCTTCCGGTATTGCTAGGG

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diamino pimelate--D-alanyl-D-alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MKMQVAEIAKAVQAVNAEQAWPDVNVTGVAFDSRKLQAGDLFIPLMGANDGHQYIQSAIEHGAVATLWASDHADDVPEDL
PVIMVADTLTALQQLGQYYLQKINPKVVAVTGSNGKTTTKDMIASVLSTQFNVTKTHANFNNQIGVPVTLLSMEPNTEVV
VVEMGMDHFGELDFLSRLVQPDVAVITMIGEAHIEFFGTRDRIADAKMEIVHGLKEDGTFIFNGDEPLLQERAKEVSQHQ
RTFGLDDTNTLAGSDITTSRAQTQFKTSLWPDNTYTIPMMGAYNVNNALAALLVADTFHIRPAAAQQAIASFVPTENRTE
WLTGYRGEAILSDVYNSNPTAARRVLAAFAAVPTKGQRIAVLGDMLELGDQSDALHMSLASELDPATIASVYLNGEHMRA
LAEVLQEKYPAGAVHYYQTDQQPQLIADLTATVSADDQVLLKGSHGIHLEKVLTALEAAPEK

Sequences:

>Translated_462_residues
MKMQVAEIAKAVQAVNAEQAWPDVNVTGVAFDSRKLQAGDLFIPLMGANDGHQYIQSAIEHGAVATLWASDHADDVPEDL
PVIMVADTLTALQQLGQYYLQKINPKVVAVTGSNGKTTTKDMIASVLSTQFNVTKTHANFNNQIGVPVTLLSMEPNTEVV
VVEMGMDHFGELDFLSRLVQPDVAVITMIGEAHIEFFGTRDRIADAKMEIVHGLKEDGTFIFNGDEPLLQERAKEVSQHQ
RTFGLDDTNTLAGSDITTSRAQTQFKTSLWPDNTYTIPMMGAYNVNNALAALLVADTFHIRPAAAQQAIASFVPTENRTE
WLTGYRGEAILSDVYNSNPTAARRVLAAFAAVPTKGQRIAVLGDMLELGDQSDALHMSLASELDPATIASVYLNGEHMRA
LAEVLQEKYPAGAVHYYQTDQQPQLIADLTATVSADDQVLLKGSHGIHLEKVLTALEAAPEK
>Mature_462_residues
MKMQVAEIAKAVQAVNAEQAWPDVNVTGVAFDSRKLQAGDLFIPLMGANDGHQYIQSAIEHGAVATLWASDHADDVPEDL
PVIMVADTLTALQQLGQYYLQKINPKVVAVTGSNGKTTTKDMIASVLSTQFNVTKTHANFNNQIGVPVTLLSMEPNTEVV
VVEMGMDHFGELDFLSRLVQPDVAVITMIGEAHIEFFGTRDRIADAKMEIVHGLKEDGTFIFNGDEPLLQERAKEVSQHQ
RTFGLDDTNTLAGSDITTSRAQTQFKTSLWPDNTYTIPMMGAYNVNNALAALLVADTFHIRPAAAQQAIASFVPTENRTE
WLTGYRGEAILSDVYNSNPTAARRVLAAFAAVPTKGQRIAVLGDMLELGDQSDALHMSLASELDPATIASVYLNGEHMRA
LAEVLQEKYPAGAVHYYQTDQQPQLIADLTATVSADDQVLLKGSHGIHLEKVLTALEAAPEK

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=451, Percent_Identity=31.9290465631929, Blast_Score=164, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 50182; Mature: 50182

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMQVAEIAKAVQAVNAEQAWPDVNVTGVAFDSRKLQAGDLFIPLMGANDGHQYIQSAIE
CCCCHHHHHHHHHHHCHHHCCCCCCEEEEEECCCEEECCCEEEEEECCCCHHHHHHHHHH
HGAVATLWASDHADDVPEDLPVIMVADTLTALQQLGQYYLQKINPKVVAVTGSNGKTTTK
CCCEEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
DMIASVLSTQFNVTKTHANFNNQIGVPVTLLSMEPNTEVVVVEMGMDHFGELDFLSRLVQ
HHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHC
PDVAVITMIGEAHIEFFGTRDRIADAKMEIVHGLKEDGTFIFNGDEPLLQERAKEVSQHQ
CCEEEEEEECCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHH
RTFGLDDTNTLAGSDITTSRAQTQFKTSLWPDNTYTIPMMGAYNVNNALAALLVADTFHI
HHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCC
RPAAAQQAIASFVPTENRTEWLTGYRGEAILSDVYNSNPTAARRVLAAFAAVPTKGQRIA
CHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEE
VLGDMLELGDQSDALHMSLASELDPATIASVYLNGEHMRALAEVLQEKYPAGAVHYYQTD
EEHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECC
QQPQLIADLTATVSADDQVLLKGSHGIHLEKVLTALEAAPEK
CCCHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MKMQVAEIAKAVQAVNAEQAWPDVNVTGVAFDSRKLQAGDLFIPLMGANDGHQYIQSAIE
CCCCHHHHHHHHHHHCHHHCCCCCCEEEEEECCCEEECCCEEEEEECCCCHHHHHHHHHH
HGAVATLWASDHADDVPEDLPVIMVADTLTALQQLGQYYLQKINPKVVAVTGSNGKTTTK
CCCEEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHH
DMIASVLSTQFNVTKTHANFNNQIGVPVTLLSMEPNTEVVVVEMGMDHFGELDFLSRLVQ
HHHHHHHHCCCEEEEEECCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHC
PDVAVITMIGEAHIEFFGTRDRIADAKMEIVHGLKEDGTFIFNGDEPLLQERAKEVSQHQ
CCEEEEEEECCCEEEEECCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHH
RTFGLDDTNTLAGSDITTSRAQTQFKTSLWPDNTYTIPMMGAYNVNNALAALLVADTFHI
HHCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHCC
RPAAAQQAIASFVPTENRTEWLTGYRGEAILSDVYNSNPTAARRVLAAFAAVPTKGQRIA
CHHHHHHHHHHHCCCCCCCHHHCCCCCCHHHHHHHCCCCHHHHHHHHHHHHCCCCCCEEE
VLGDMLELGDQSDALHMSLASELDPATIASVYLNGEHMRALAEVLQEKYPAGAVHYYQTD
EEHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEECC
QQPQLIADLTATVSADDQVLLKGSHGIHLEKVLTALEAAPEK
CCCHHHHHHHHEECCCCEEEEECCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]