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The map label for this gene is glmU

Identifier: 28377365

GI number: 28377365

Start: 418119

End: 419501

Strand: Direct

Name: glmU

Synonym: lp_0467

Alternate gene names: 28377365

Gene position: 418119-419501 (Clockwise)

Preceding gene: 28377364

Following gene: 28377366

Centisome position: 12.64

GC content: 47.22

Gene sequence:

>1383_bases
ATGACAACCAAAAATGCAATCATCATGGCCGCTGGTAAAGGGACCCGGATGAAATCCAAGCTAGTCAAAGTATTACATCA
GGTCTGTGGCAAATCGATGGTCGACCACGTCTTGACCCAAGTTGAAGCCACCCACATGGCAAATATCGTGACCATTGTAG
GCCACGGTGCCAAAGATGTGGAAGCAGCCTTAGGCGACCGAACTGAGTACGCGGTTCAAACGGAACAATTAGGAACCGGT
CACGCAGTATTACAAGCCGAATCGCTCTTAAAAGATGCGGATGGGATGACGCTGATCGTCAGTGGCGACACACCCTTATT
TAAAGCTGAAACTTTTGAAGAATTATTTGAATATCATCAAGCTAAGGGTGCTGCTGGCACGATTTTAACGTCCAAGGCAC
CTAATCCTCAAGGCTACGGCCGGGTTGTTCGCAATCGGTTAGGCATCGTTGAAAAGATTGTAGAACAAAAGGATGCGACT
AAGGAAGAACAAGAAATTCATGAAATTAACACGGGGGTCTACTGCTTCGATAATCAGAAGTTGTTTGCCGCCTTACATGA
AGTGACCAACGATAATGCACAGGGCGAATATTATTTGACTGACGTGATTCAGATCATGAAGAATCAGGGTGATGTGGTCG
CCGCCTATCAAATGGATGATTTTGATGAATCAATGGGGGTAAATACCCGTGCAGCTCTAGCCCAAGCAACCAAAGTGATG
CAACAACGGATCAACGCGCAGCATATGGAAAATGGAGTTTCAATTATCAACCCTGACGATACCTATATTGATGCGGGCGT
TAAGATTGGTGCTGATACGATTATTGAACCAGGCGTCTTGATCAAGGGCCATACGACAATTGGTGAAGATTGCTTCATCG
GCGCGCACTCCGAGATCCATGACATGGTGATTGAAGATCGTGTTCGGGTGACGGCTTCCTTCTTGGAAGATTCCATCATG
CACGCTGACAGCAACATTGGCCCGTATAGCCATTTGCGGCCGCAAGCTGAGATTGGTGAACACGTCCATCTTGGTAACTT
TGTCGAAGTCAAGAAGGCTAAGATCGGTAATCGGACCAAGGTCGGCCATTTGACTTATGTTGGTGACGCAACGTTAGGTC
AAGATATTAACGTTGGTTGTGGCGTGGTCTTCGTTAACTATGACGGAGTCAACAAGCACCACACGAATGTCGGCGATTCC
GCCTTTATTGGTAGTAACTCGAACATCATTGCGCCAGTTGAAGTGGCTGACCACTCGTTTATCGCGGCTGGTTCGACCAT
TACTGATGATGTTAACTTCCATGATATGGCAATTGCCCGGGCACGTCAGACGAACAAGCCCGACTTCTGGGGCCGTTTAC
CACATGAACCAGAAAACATGTAA

Upstream 100 bases:

>100_bases
ATTTGGAGAAGGTTTTCGGTGCGGAAGTCTAGCCTGGTGTGTTATCCAGACCAGTTTTTTGATATGATGGACTTATCTGA
GGACTAAGGAGTAATTTGTA

Downstream 100 bases:

>100_bases
TTATTATCATTGATAATTGAATTCAGAGACCATCCAAGTGCGTTGTTGCTGGGATGGTCTTTTTTTGTTAGCTCATCGGC
GTGACTTGTGGTGTAATAAG

Product: bifunctional N-acetylglucosamine-1-phosphate uridyltransferase/glucosamine-1-phosphate acetyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTTKNAIIMAAGKGTRMKSKLVKVLHQVCGKSMVDHVLTQVEATHMANIVTIVGHGAKDVEAALGDRTEYAVQTEQLGTG
HAVLQAESLLKDADGMTLIVSGDTPLFKAETFEELFEYHQAKGAAGTILTSKAPNPQGYGRVVRNRLGIVEKIVEQKDAT
KEEQEIHEINTGVYCFDNQKLFAALHEVTNDNAQGEYYLTDVIQIMKNQGDVVAAYQMDDFDESMGVNTRAALAQATKVM
QQRINAQHMENGVSIINPDDTYIDAGVKIGADTIIEPGVLIKGHTTIGEDCFIGAHSEIHDMVIEDRVRVTASFLEDSIM
HADSNIGPYSHLRPQAEIGEHVHLGNFVEVKKAKIGNRTKVGHLTYVGDATLGQDINVGCGVVFVNYDGVNKHHTNVGDS
AFIGSNSNIIAPVEVADHSFIAAGSTITDDVNFHDMAIARARQTNKPDFWGRLPHEPENM

Sequences:

>Translated_460_residues
MTTKNAIIMAAGKGTRMKSKLVKVLHQVCGKSMVDHVLTQVEATHMANIVTIVGHGAKDVEAALGDRTEYAVQTEQLGTG
HAVLQAESLLKDADGMTLIVSGDTPLFKAETFEELFEYHQAKGAAGTILTSKAPNPQGYGRVVRNRLGIVEKIVEQKDAT
KEEQEIHEINTGVYCFDNQKLFAALHEVTNDNAQGEYYLTDVIQIMKNQGDVVAAYQMDDFDESMGVNTRAALAQATKVM
QQRINAQHMENGVSIINPDDTYIDAGVKIGADTIIEPGVLIKGHTTIGEDCFIGAHSEIHDMVIEDRVRVTASFLEDSIM
HADSNIGPYSHLRPQAEIGEHVHLGNFVEVKKAKIGNRTKVGHLTYVGDATLGQDINVGCGVVFVNYDGVNKHHTNVGDS
AFIGSNSNIIAPVEVADHSFIAAGSTITDDVNFHDMAIARARQTNKPDFWGRLPHEPENM
>Mature_459_residues
TTKNAIIMAAGKGTRMKSKLVKVLHQVCGKSMVDHVLTQVEATHMANIVTIVGHGAKDVEAALGDRTEYAVQTEQLGTGH
AVLQAESLLKDADGMTLIVSGDTPLFKAETFEELFEYHQAKGAAGTILTSKAPNPQGYGRVVRNRLGIVEKIVEQKDATK
EEQEIHEINTGVYCFDNQKLFAALHEVTNDNAQGEYYLTDVIQIMKNQGDVVAAYQMDDFDESMGVNTRAALAQATKVMQ
QRINAQHMENGVSIINPDDTYIDAGVKIGADTIIEPGVLIKGHTTIGEDCFIGAHSEIHDMVIEDRVRVTASFLEDSIMH
ADSNIGPYSHLRPQAEIGEHVHLGNFVEVKKAKIGNRTKVGHLTYVGDATLGQDINVGCGVVFVNYDGVNKHHTNVGDSA
FIGSNSNIIAPVEVADHSFIAAGSTITDDVNFHDMAIARARQTNKPDFWGRLPHEPENM

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-GlcNAc. Responsible for the acetylation of Glc-N-1-P to give GlcNAc-1-P and for the uridyl transfer from UTP to GlcNAc-1-P which produces UDP-GlcNAc

COG id: COG1207

COG function: function code M; N-acetylglucosamine-1-phosphate uridyltransferase (contains nucleotidyltransferase and I-patch acetyltransferase domains)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family

Homologues:

Organism=Escherichia coli, GI1790168, Length=450, Percent_Identity=40.4444444444444, Blast_Score=319, Evalue=3e-88,
Organism=Saccharomyces cerevisiae, GI6320148, Length=363, Percent_Identity=21.763085399449, Blast_Score=70, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLMU_LACPL (Q88Z86)

Other databases:

- EMBL:   AL935253
- RefSeq:   NP_784257.1
- ProteinModelPortal:   Q88Z86
- SMR:   Q88Z86
- GeneID:   1064143
- GenomeReviews:   AL935263_GR
- KEGG:   lpl:lp_0467
- NMPDR:   fig|220668.1.peg.391
- HOGENOM:   HBG688195
- OMA:   GSKVNHL
- ProtClustDB:   PRK14354
- BioCyc:   LPLA220668:LP_0467-MONOMER
- BRENDA:   2.3.1.157
- BRENDA:   2.7.7.23
- GO:   GO:0005737
- HAMAP:   MF_01631
- InterPro:   IPR005882
- InterPro:   IPR018357
- InterPro:   IPR005835
- InterPro:   IPR011004
- TIGRFAMs:   TIGR01173

Pfam domain/function: PF00483 NTP_transferase; SSF51161 Trimer_LpxA_like

EC number: =2.7.7.23; =2.3.1.157

Molecular weight: Translated: 50081; Mature: 49950

Theoretical pI: Translated: 5.38; Mature: 5.38

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: ACT_SITE 363-363 BINDING 73-73 BINDING 140-140 BINDING 155-155 BINDING 170-170 BINDING 387-387 BINDING 405-405 BINDING 423-423 BINDING 440-440

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKNAIIMAAGKGTRMKSKLVKVLHQVCGKSMVDHVLTQVEATHMANIVTIVGHGAKDV
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHH
EAALGDRTEYAVQTEQLGTGHAVLQAESLLKDADGMTLIVSGDTPLFKAETFEELFEYHQ
HHHHCCCCHHEEEHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHH
AKGAAGTILTSKAPNPQGYGRVVRNRLGIVEKIVEQKDATKEEQEIHEINTGVYCFDNQK
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCH
LFAALHEVTNDNAQGEYYLTDVIQIMKNQGDVVAAYQMDDFDESMGVNTRAALAQATKVM
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCHHHHHHHHHHHH
QQRINAQHMENGVSIINPDDTYIDAGVKIGADTIIEPGVLIKGHTTIGEDCFIGAHSEIH
HHHHCHHHHHCCCEEECCCCCEEECCCEECCCCCCCCCEEEECCCCCCCCEEECCCHHHH
DMVIEDRVRVTASFLEDSIMHADSNIGPYSHLRPQAEIGEHVHLGNFVEVKKAKIGNRTK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCEEECCCEEEEHHHHCCCCCE
VGHLTYVGDATLGQDINVGCGVVFVNYDGVNKHHTNVGDSAFIGSNSNIIAPVEVADHSF
EEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCEEEEEEECCCCE
IAAGSTITDDVNFHDMAIARARQTNKPDFWGRLPHEPENM
EECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTKNAIIMAAGKGTRMKSKLVKVLHQVCGKSMVDHVLTQVEATHMANIVTIVGHGAKDV
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHH
EAALGDRTEYAVQTEQLGTGHAVLQAESLLKDADGMTLIVSGDTPLFKAETFEELFEYHQ
HHHHCCCCHHEEEHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHH
AKGAAGTILTSKAPNPQGYGRVVRNRLGIVEKIVEQKDATKEEQEIHEINTGVYCFDNQK
CCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEECCCH
LFAALHEVTNDNAQGEYYLTDVIQIMKNQGDVVAAYQMDDFDESMGVNTRAALAQATKVM
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCHHHHHHHHHHHH
QQRINAQHMENGVSIINPDDTYIDAGVKIGADTIIEPGVLIKGHTTIGEDCFIGAHSEIH
HHHHCHHHHHCCCEEECCCCCEEECCCEECCCCCCCCCEEEECCCCCCCCEEECCCHHHH
DMVIEDRVRVTASFLEDSIMHADSNIGPYSHLRPQAEIGEHVHLGNFVEVKKAKIGNRTK
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCHHHHCCEEECCCEEEEHHHHCCCCCE
VGHLTYVGDATLGQDINVGCGVVFVNYDGVNKHHTNVGDSAFIGSNSNIIAPVEVADHSF
EEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEEECCCCCEEEEEEECCCCE
IAAGSTITDDVNFHDMAIARARQTNKPDFWGRLPHEPENM
EECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12566566